VSD负压及封闭引流技术在四肢电烧伤创面治疗中的应用

目的 V8D负压封闭引流技术在四肢电烧伤创面治疗中的应用及疗效.疗法2008年12月开始,临床收治四肢电烧伤30例,急诊手术清创后应用持续封闭负压引流敷料覆盖创面,吸引器持续负压吸引治疗7～10天后,拆除VSD后行二期植皮或皮瓣转移术.结果 30例患者20例患者一次使用VSD治疗后行二期植皮或皮瓣转移全部存活,10例患者行两次VSD治疗后植皮全部存活.结论 四肢电烧伤创面早期清创后VSD外敷行二期植皮或皮瓣转移获得满意疗效,是一种理想的治疗方法.     

持续封闭负压引流技术在急诊创伤中的应用

目的探讨持续封闭负压引流(vacuum sealing drainage,VSD)技术在急诊创伤的临床应用。方法选择我科2011年4—10月收治的急诊创伤皮肤软组织缺损患者61例,行急诊手术清创,应用持续封闭负压引流敷料覆盖创面,吸引器持续吸引治疗7～8d后,拆除敷料行二期植皮术。结果 61例患者中58例使用VSD治疗后行二期植皮全部成活,3例行2次VSD治疗后植皮全部成活。结论 VSD能彻底清除创面的分泌物和坏死组织,刺激肉芽组织生长,明显缩短创伤性皮肤软组织损伤的治疗时间,减轻换药痛苦,手术简单,疗效确切。     

VSD负压封闭引流治疗四肢软组织严重创伤的临床观察

目的：探讨持续封闭负压引流（vacuum sealing drainage,VSD）技术治疗四肢皮肤及软组织严重创伤的临床效果。方法：对我院2008年3月—2010年5月临床收治的28例四肢软组织严重创伤患者行手术清创,应用持续封闭负压引流敷料覆盖创面,吸引器持续吸引治疗后,拆除敷料行二期植皮或皮瓣转移术。结果：20例患者一次使用VSD治疗二期植皮或皮瓣转移一次性成活,8例患者行两次VSD治疗后植皮全部成活。结论：VSD技术能明显促进创面肉芽生长,帮助控制创面感染,有效解决深部组织的临时覆盖,为最终关闭创面创造良好条件,是治疗四肢严重软组织损伤创面安全有效的方法。     

负压封闭引流治疗患者骶尾部褥疮疗效分析

目的 观察负压封闭引流(VSD)治疗骶尾部褥疮的临床疗效.方法 选取26例骶尾部褥疮患者行一期清创手术后放置负压封闭引流装置7~10 d,联合药物及营养支持等治疗.结果 患者经负压封闭引流治疗2~3个周期后,5例患者创面愈合,12例患者创口肉芽生长良好,经中厚皮片植皮后愈合,剩余6例患者经臀部穿支皮瓣转移,创口一期愈合.结论 VSD是治疗骶尾部褥疮非常有效的方式,具有较好的临床应用前景.     

应用负压封闭引流结合游离植皮治疗巨大骶部褥疮

目的 探索负压封闭引流(Vacuum sealing drainage,VSD)技术结合游离植皮治疗骶部巨大褥疮的疗效.方法 对10例骶部巨大褥疮的患者经有效的清创、持续负压封闭引流,观察创面肉芽组织生长情况,待创面肉芽生长良好后行游离植皮修复创面.结果 10例患者经过1-2次VSD负压引流,治疗2-4周,创面坏死组织脱落,骶部创面肉芽组织新鲜,游离植皮皮片全部成活.外观、功能恢复良好.结论 VSD结合游离植皮治疗巨大骶部褥疮减少了褥疮患者的死亡率,是一种操作简单、损伤小、安全有效的方法.     

持续封闭负压引流技术治疗复杂创伤性皮肤软组织缺损的临床疗效观察

目的观察持续封闭负压引流（vacuum-sealing drainage,VSD）技术治疗复杂创伤性皮肤软组织缺损的临床效果。方法对2007年2月-2008年8月临床收治的23例复杂创伤性皮肤软组织缺损行手术清创,应用持续封闭负压引流敷料覆盖创面,吸引器持续吸引治疗6～8天后,拆除敷料行二期植皮术。结果23例患者中21例患者使用VSD治疗后行二期植皮全部成活;2例患者行两次VSD治疗后植皮全部成活。结论VSD能彻底清除创面的分泌物和坏死组织,刺激肉芽生长,明显缩短创伤性皮肤软组织缺损的治疗时间,减轻换药痛苦,效果显著。     

VSD负压密封引流技术在四肢软组织严重创伤治疗中的应用

目的探讨持续封闭负压引流（vacuum-sealing drainage,VSD）技术治疗四肢皮肤及软组织严重创伤治疗的临床效果。方法对2007年9月至2009年10月临床收治的13例复杂创伤性皮肤软组织缺损行手术清创,应用持续封闭负压引流敷料覆盖创面,吸引器持续吸引治疗7～10d后,拆除敷料行二期植皮或皮瓣转移术。结果13例患者中11例患者一次使用VSD治疗后行二期植皮或皮瓣转移术全部成活;2例患者行两次VSD治疗后植皮全部成活。结论VSD能彻底清除创面的分泌物和坏死组织,刺激肉芽生长,减少创面感染,明显缩短创伤性皮肤软组织缺损的治疗时间,疗效显著。     

负压封闭引流术(VSD)在骨科临床应用中的护理体会

目的:总结骨科患者负压封闭引流术(VSD)的术前术后护理方法。方法:对我科临床收治的14例复杂创伤性皮肤软组织缺损及开放性骨折术后感染行手术清创,应用持续封闭负压引流敷料覆盖创面,中心负压持续吸引治疗5~7 d后,拆除敷料(必要可更换VSD敷料),然后行游离植皮术。结果:14例患者经过精心护理,13例患者使用VSD治疗后一次性植皮或行Ⅱ期缝合成功,1例患者行了3次VSD治疗后,植皮成活,效果满意,无并发症发生。结论:采用VSD治疗损伤创面能彻底清除创面的分泌物和坏死组织,减少机体对坏死组织及毒素的重吸收,减轻局部水肿,改善局部血液循环,促进肉芽组织生长,缩短了创面愈合时间,减少了患者的综合医疗费用,是一种理想的引流方法,值得临床推广使用。     

创面封闭式负压引流(VSD)对四肢皮肤缺损组织外露创面疗效的影响

目的:观察创面持续封闭负压引流(vacuum sealing drainage,VSD)对四肢皮肤缺损软组织外露创面疗效的临床效果。方法:对2010年2月～2011年2月临床收治的28例复杂创伤性皮肤软组织缺损患者行手术清创,随机分为观察组与对照组,各19例,观察组患者应用持续封闭负压引流敷料覆盖创面,吸引器持续吸引治疗6～8天后,拆除敷料行二期植皮术。对照组患者给予常规清创包扎直到创面肉芽生长良好后植皮治疗。结果:观察组19例患者治疗后全部成活;对照组19例患者11例一次手术植皮成活,其余8例患者经二次手术植皮成活。结论:VSD能彻底清除创面的分泌物和坏死组织,刺激肉芽生长,明显缩短创伤性皮肤软组织缺损的治疗时间,减轻换药痛苦,效果显著。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.