共查询到20条相似文献，搜索用时 31 毫秒
1.
Benoît Bataille Carine Chan-Shun Bastian Nucci Bernard Verdoux Michel Mora Pierre Cocquet Stein Silva 《中医杂志(英文版)》2017,37(4):554-557
Objective
To observe the effect of transcutaneous electroacupuncture (TEA) at Neiguan (PC 6) on refractory vomiting in critically ill patients in intensive care (ICU) setting.Methods
Ten patients admitted in ICU and presenting vomiting refractory to one or more antiemetic drugs were prospectively included in the study. TEA was applied at acupoint of Neiguan (PC 6) during 30 min with a neuromuscular transmission monitor (single-twitch stimulation with 1 Hz at a constant current of 10 mA). Nausea and Vomiting were evaluated at the following intervals: immediately after 30 min of TEA at Neiguan (PC 6), 30 min-6h and 6–24 h. The presence of nausea and/or vomiting throughout the observational period was defined as the primary end point.Results
The presence of nausea or vomiting throughout the observational period was 10% at the end of TEA, 40% between 30 min and 6 h, and 50% between 30 min and 24 h (P < 0.001, P = 0.01 and P = 0.03 vs pre-TEA, respectively). There were no complications or side effects related to TEA.Conclusion
TEA at Neiguan (PC 6) seems effective in reducing refractory vomiting in the patients in ICU setting, even if larger trials are needed to define optimal modalities. 相似文献2.
Lu Fengyan Gao Junhong Wang Yuanyuan Dai Qiufu Bao Yan Hong Yang Zhang Guolei Xin Juanjuan Zhao Yuxue Wu Shuyang Chen Yuanyuan Li Xiaojiao Fang Jiliang Yu Xiaochun 《中医杂志(英文版)》2017,37(3):298-307
Objective
To investigate the effects on the brain using three needling manipulations (twirling, lifting-thrusting, and twirling plus lifting-thrusting) when the right-side Zusanli (ST 36) acupoint was stimulated with needles.Methods
Seventeen healthy subjects accepted three needling manipulations stimulating the right Zusanli (ST 36) over separate days. Functional magnetic resonance imaging was used to detect changes in the brain during the manipulations, and then the needling sensations were recorded using the MGH acupuncture sensation scale (MASS) after each scan. fMRI data were processed using Statistical Parametric Mapping 8 to analyze the positive and negative activation in the brain induced by different acupuncture manipulations.Results
The individual needling sensations showed no statistically significant differences among the three manipulations. However, the MASS index showed that lifting-thrusting > twirling plus lifting-thrusting > twirling. Lifting-thrusting activated left premotor cortex, left postcentral, right middle frontal gyrus, left inferior frontal gyrus, right lingual gyrus, left insula, right putamen, bilateral cingulate gyrus and right cerebellum; and deactivated bilateral hippocampus and left caudate. Twirling activated bilateral orbital middle frontal gyrus, left opercular and triangular inferior frontal gyrus, and right middle occipital gyrus; and deactivated bilateral precuneus, right amygdala, left anterior cingulate gyrus, right inferior temporal gyrus, right middle frontal gyrus, right supplementary motor gyrus, and left postcentral. Twirling plus lifting-thrusting activated bilateral postcentral, left inferior occipital gyrus, left insula, left thalamus, left cingulate gyrus, and right putamen; and deactivated right superior frontal gyrus, right superior parietal gyrus, right temporal gyrus, right middle occipital gyrus, right insula, and left lingual gyrus. Pairwise comparisons of the three manipulations showed that signals induced by lifting-thrusting were the strongest, especially in the limbic system, followed by twirling plus lifting-thrusting; twirling alone was the weakest.Conclusion
Three methods of needling manipulations similarly activated areas associated with the somatosensory system, vision, cognition, and emotional regulation. This may have significant implications for acupuncture in clinical practice. 相似文献3.
Wang Jing Cheng Kai Qin Zhuo Wang Yanping Zhai Lijing You Min Wu Juanjiao 《中医杂志(英文版)》2017,37(1):96-100
OBJECTIVE
To investigate the effects of electroacupuncture (EA) at the Guanyuan (CV 4) or Sanyinjiao (SP 6) acupoints on the hypothalamus-pituitary-ovary (HPO) axis and spatial learning and memory in female mice.METHODS
Nine-month-old female mice with senescence-accelerated mouse prone 8 (SAMP8) were divided into three groups: the disease model, EA-Guanyuan and EA-Sanyinjiao groups. Concurrently, 9-month old female mice with senescence-accelerated mouse resistance 1 (SAMR1) were set as the control model group. The two treatment groups were given the same pattern of EA stimulation. Gonadotropin-releasing hormone, luteinizing hormone, follicle-stimulating hormone (FSH) and Serum estradiol levels in the Hypothalamus-pituitary-ovary axis were assessed by enzyme-linked immunosorbent assay to determine the HPO axis function level. Spatial learning and memory were assessed by the Morris Water Maze (MWM) test.RESULTS
(a) HPO axis: compared with the control model group, the disease model group displayed a decrease in E2 levels (P < 0.01), and an increase in GnRH, LH and FSH levels (P < 0.01). E2 levels were increased in EA treatment groups compared with the disease model group (P < 0.05). In contrast, GnRH and LH and FSH levels were reduced (P < 0.05). EA-Sanyinjiao group was superior than EA-Guanyuan group on increasing E2 and declining GnRH levels (P < 0.01). (b) The MWM test demonstrated that the response latency in the EA-Sanyinjiao treatment group declined from day 2 to day 5 compared with the disease model group (P < 0.05), whereas the EA-Guanyuan treatment group showed no significant difference.CONCLUSION
EA can regulate hormone (E2, FSH, LH, GnRH) levels in the HPO axis and the spatial learning and memory ability in female SAMP8 mice. Moreover, this effect may have been more pronounced in the EA-Sanyinjiao group than the EA-Guanyuan group. The underlying mechanism of the EA-induced changes may be related to gonadal hormone shifts in the HPO axis, followed by an improvement in spatial learning and memory. 相似文献4.
Fan Wentao Huang Yuwei Wang Lisheng Liao Weiguo Li Zhou Wu Yinai Liao Fengyun Yu Jianye Liu Qiang 《中医杂志(英文版)》2017,37(4):503-509
Objective
To investigate the effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.Methods
Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu (BL 13) acupoint group, the Dazhui (GV 14) acupoint group, and the nonacupoint group or control group. Using microdialysis technology, preprocessed skin probes were implanted into the rats at Feishu (BL 13), Dazhui (GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu (BL 13), Dazhui (GV 14) acupoints, and the nonacupoint for all groups. Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.Results
The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics (r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.Conclusion
Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood. 相似文献5.
She Yanfen Ma Liangxiao Zhu Jiang Qi Conghui Wang Yanxia Tang Ling Li Chunhua Yuan Hongwen Liu Yuqi Song Jiashan 《中医杂志(英文版)》2017,37(2):220-228
Objective
To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea (PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.Methods
Fifty-two PD patients and 49 healthy volunteers were recruited. Skin temperature measurements were performed with a skin temperature assessment device at 10 points. Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.Results
On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi (KI 3) compared with the healthy group (P < 0.01). A significant reduction in skin temperature difference was detected at Taixi (KI 3) in the first day of menstruation compared with those values in the third day after menstruation (P < 0.01) in the healthy group. On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu (LR 6) in PD group compared with the healthy group (P < 0.05). No significant differences of skin temperature were detected at other points (P > 0.05).Conclusion
The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD. Significant difference was only found in Taixi (KI 3), the Yuan-source point of Kidney meridian. 相似文献6.
Objective
To investigate the effect of electroacupuncture (EA) at acupoints on the pericardium meridian on the expression of phosphorylated Akt (p-Akt) protein in rat myocardium after ischemia and reperfusion.Methods
Seventy Wistar rats were evenly randomized into seven groups: the sham operation group (group A), ischemia-reperfusion model I group (group B), ischemia-reperfusion model II group (group C), EA at Neiguan (PC 6) group (group D), EA at Ximen (PC 4) group (group E), EA at Hegu (LI 4) group (group F), and LY294002 + EA at Neiguan (PC 6) group (group G). All processes were monitored by electrocardiography. In group A, the left anterior descending coronary artery was only threaded without ligation for 100 min. In group B, the left anterior descending coronary artery was ligated for 40 min and reperfused for 60 min. The left anterior descending coronary artery in group C was ligated for 40 min and reperfused for 100 min. Groups D, E, and F received EA for 20 min before undergoing ischemia for 40 min, and then received EA for 20 min before undergoing reperfusion for 60 min. Before modeling, group G was injected with LY294002 (0.3 mg/kg) into the tail vein, and then underwent the same intervention as the other EA groups. After reperfusion, myocardial tissue from the left cardiac ventricle was collected to enable Western blot analysis of the p-Akt level, and analysis of electrocardiographic changes.Results
In groups B and C, electrocardiography showed obvious elevation of the ST-segment II lead (ECG-STII), while the ECG-STII values were significantly lower in groups D, E, and G (P < 0.01). The p-Akt levels in groups D and E were significantly greater than those in groups B and C (P < 0.01). Compared with all other groups, group G showed a significantly different expression of p-Akt (P < 0.01).Conclusion
The expression of p-Akt protein in cardiomyocytes was significantly greater in rats that were injected with LY294002 and received EA at Ximen (PC 4) compared with all other groups. This suggests that EA at Ximen (PC 4) resulted in activation of the phosphoinositide 3-kinase/Akt signaling pathway and phosphorylation of Akt. 相似文献7.
Objective
To evaluate the clinical efficacy and safety of five phase music therapy in patients with depression after ischemic stroke.Methods
A total of 92 patients with post-stroke depression were randomly divided into the control group (32 cases), treatment group A (30 cases), and treatment group B (30 cases). All groups were given basic therapies for cerebral infarction. In addition, the control group was administerd 50 mg of oral sertraline hydrochloride daily, while treatment groups A and B received needling at Baihui (GV 20) plus acupoint injection at Yanglingquan (GB 34) daily; treatment group B also received music therapy derived from the five phases in Traditional Chinese Medicine theory twice daily. All treatments were administered for 5 days per treatment cycle for three cycles, with a 1 day interval between cycles. In all three groups, Hamilton's depression scale (HAMD-17) score and the activities of daily life (ADL) score were measured before and after treatment, and side effects were assessed with the Treatment Emergent Symptom Scale.Results
The HAMD-17 score significantly decreased after treatment in all three groups, and the post-treatment reduction in HAMD-17 score was markedly greater in treatment group B than in treatment group A (P < 0.01). The ADL score significantly increased after treatment in all three groups, and the post-treatment increase in ADL score was significantly greater in treatment group B than in treatment group A (P < 0.01). The Treatment Emergent Symptom Scale score was highest in the control group, and lowest in group B, and significantly differed between the three groups (P < 0.01).Conclusion
Five phase music therapy plus acupoint needling and acupoint injection can improve the symptoms in patients with post-stroke depression. 相似文献8.
Li Jing Bai Zonglu Du Yuanhao Li Yongfeng Zhang Xuezhu Pang Bo Zhang Jingjing 《中医杂志(英文版)》2017,37(5):659-666
Objective
The aim of this study is identify the intervention mechanism of the effects of electro-acupuncture on the expression of Ang/Tie-2 mRNA and protein in rats with acute cerebral infarction induced by middle cerebral artery occlusion (MCAO).Methods
Altogether 120 Wistar rats were subjected to MCAO by inserting a nylon filament, and then divided into 3 groups: control group, injured group and electro-acupuncture group. The injured and electro-acupuncture groups were further divided into the following 7 subgroups according to the time after MCAO: 3, 6, 12, 24 h, 3, 7 and 12 day, with 8 rats in each subgroup. The electro-acupuncture group was given electro-acupuncture treatment at Shuigou (GV 26) instantly after operation. The rats were killed at different time points according to their groups, and then the expression levels of Ang/Tie-2 mRNA and protein were detected using Real-Time PCR and immunohistochemical staining.Results
The mRNA and protein expression levels of Ang/Tie-2 in the electro-acupuncture group were significant higher than that in the injured group.Conclusion
The results suggested that electro-acupuncture could significantly regulate the expression of Ang/Tie-2 mRNA and protein in the rats with acute cerebral infarction induced by MCAO, and enhance angiogenesis after ischemic penumbra. 相似文献9.
Wang Dan Ji Bo Zhao Guozhen Yan Mingna Bai Hongxin Sun Xiaomin Dai Jian Lu Yawen Liu Yitian You Min Wu Jiaojuan Ge Yunpeng Su Hang Cheng Kai 《中医杂志(英文版)》2017,37(6):794-803
Objective
To investigate the effect of electroacupuncture (EA) of acupoints Neiguan (PC 6) and Tianquan (PC 2) on the skin temperature, blood perfusion, and alpha1- and beta2-adrenoreceptor (α1-AR and β2-AR) protein and mRNA level in rats with acute myocardial ischemia.Methods
Thirty male adult Wistar rats [(230 ± 20) g] were randomly assigned into five groups (n = 6 each): a control group, sham operation group, model group, model group treated with EA at low frequency (L-EA, 2 Hz, 1 mA) and model group treated with EA at high frequency (H-EA, 100 Hz, 1 mA). The rat model was prepared by ligating the left anterior descending coronary artery. Electroacupuncture was performed at the left Neiguan (PC 6) for 20min daily for 3 d. After the 3rd time of the treatment, measurements of skin blood perfusion and temperature in Neiguan (PC 6) and Tianquan (PC 2) in all groups were made by laser speckle contrast imaging and infrared thermal image instrument. Real-time PCR and ELISA were used to measure mRNA level and protein level of α1-AR and β2-AR in the skin tissues of Neiguan (PC 6) and Tianquan (PC 2), respectively. Serum levels of cTnT and electrocardiogram were used to identify the state of myocardium.Results
In the group receiving electroacupunture at Neiguan (PC 6), compared with control group, the skin temperature, blood perfusion, and β2-AR mRNA and protein level of model group significantly decreased (P < 0.05), α1-AR mRNA and protein level of model group significantly increased (P < 0.05); compared with model group, the skin temperature, blood perfusion, and β2-AR mRNA and protein level of L-EA significantly increased (P < 0.05), α1-AR mRNA and protein level of L-EA and H-EA significantly decreased (P < 0.05). The same trend has taken place in the former four groups of the Tianquan (PC 2).Conclusion
Low-or high-frequency electroacupuncture can improve the skin temperature and blood perfusion which may be induced by decreasing the level of α1-AR and increasing the level of β2-AR of the Neiguan (PC 6) and Tianquan (PC 2) in the rat with acute myocaidial ischemia. 相似文献10.
OBJECTIVE
To investigate the effect of brain functional recovery decoction (BFRD) on expression of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) protein in rats with cerebral ischemia reperfusion injury, and to explore the mechanism of action of BFRD.METHODS
Using the suture-occlusion method, a Wistar rat model of focal cerebral ischemia reperfusion was established. The rats were randomly divided into treatment group, model group, and sham operation group. The treatment group was administered BFRD. In situ hybridization was used to detect VEGF mRNA expression. Immunohistochemistry was used to observe expression of Ang-1 protein.RESULTS
VEGF mRNA expression was greater in the model group compared with the sham operation group (P < 0.05); Ang-1 protein expression was more obvious in the treatment group than the model group (P< 0.05).CONCLUSION
BFRD promoted VEGF mRNA and Ang-1 protein expression in the brains of rats with cerebral ischemia, suggesting increased angiogenesis. 相似文献11.
Akuodor Godwin Christian Ezeonu Chinonyelum Thecla Essien Augustine Dick Asika Ebere Chile Chilaka Kingsley Chimsorom Nwachukwu Daniel Ckukwu Nwobodo Ndubuisi Nwobodo 《中医杂志(英文版)》2017,37(4):431-435
Objective
To evaluate the in vivo antiplasmodial activity and the oral acute toxicity of the Bombax buonopozense root bark aqueous extract.Methods
The in vivo antiplasmodial activity of the root bark aqueous extract of Bombax buonopozense against early and established rodent malaria infections in chloroquine sensitive Plasmodium berghei strain in mice was investigated, and oral acute toxicity of the aqueous root bark extract of Bombax buonopozense was also evaluated in mice.Results
The findings of this study revealed significant (P < 0.05) and dose dependent decrease in parasitaemia in the parasitized groups treated with varying doses of the extract (50–200 mg/kg p.o.) in both suppressive and curative tests. There was also significant decrease in parasitaemia density in the chloroquine treated group. The aqueous extract was found no toxicity in mice and the oral LD50 was determined to be greater than 5000 mg/kg.Conclusion
Bombax buonopozense root bark aqueous extract possesses potent antiplasmodial activity and may therefore, serve as potential sources of new antimalarial agents. 相似文献12.
Chen Cen Wang Fengqin Xiao Wen Xia Zhining Hu Guang Wan Jianbo Yang Fengqing 《中医杂志(英文版)》2017,37(1):64-75
OBJECTIVE
To evaluate the anti-platelet aggregation effects of extracts from 31 Traditional Chinese Medicines (TCM) with the property of activating blood and resolving stasis in terms of TCM theory.METHODS
The 31 TCMs extracts were prepared using water, 90% ethanol and ethyl acetate., and the effects on anti-platelet aggregation were tested on a platelet aggregation analyzer in vitro with adenosine 5'-diphosphate, bovine thrombin and arachidonic acid (AA) as aggregation inducers, respectively. Aspirin was the positive control.RESULTS
Lots of the tested TCMs had inhibitory effects with concentration-dependent manner on platelet aggregations induced by various agonists. Especially, some of the TCMs such as Chuanxiong (Rhizoma Chuanxiong), Yanhusuo (Rhizoma Corydalis Yanhusuo) and Danshen (Radix Salviae Miltiorrhizae) showed good anti-platelet aggregation effect similar or higher than that in positive control group.CONCLUSION
The study provided scientific references that several TCMs such as Chuanxiong (Rhizoma Chuanxiong), Yanhusuo (Rhizoma Corydalis Yanhusuo) and Danshen (Radix Salviae Miltiorrhizae), possess the property of anti-platelet aggregation. 相似文献13.
OBJECTIVE
To assess the medication adherence reporting in clinical trials the field of Traditional Chinese Medicine (TCM) and the impact factors of medication adherence.METHODS
Reviewed and evaluated were all randomized clinical trials in the field of TCM in treatment of type 2 diabetes mellitus published in Chinese journals in 2012, in terms of their medication adherence, adherence measurement, and impacted factors of adherence.RESULTS
Finally 124 studies were included. None studies reported the medication adherence. The factors impacting medication adherence couldn't be analyzed due to none reporting adherence.CONCLUSION
Medication adherence reporting was poor in clinical trials in TCM research. Establishing standards for adherence assessment and reporting may be one of the important steps to improve the quality of clinical studies. 相似文献14.
Wang Hongwei Li Hailong Chen Feng qin Luo Jun Gu Jing Wang Huping Wu Hongyan Xu Yan 《中医杂志(英文版)》2017,37(2):229-235
Objective
To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.Methods
Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells. Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.Results
In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 µmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner. Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma (Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the mRNA and protein level.Conclusion
Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells. 相似文献15.
Yang Jiamin Wu Jihui Shen Xiaoyu Zhang Mei Liu Zhenzhen Qi Dandan Zhou Jin Cui Xiao Zhang Lufen Li Yuhang 《中医杂志(英文版)》2018,38(1):76-82
OBJECTIVE
To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong (CV 17) and Ganshu (BL 18) in rats with hyperplasia of mammary gland (HMG) which induced by estrogen and progestogen.METHODS
Thirty female Sprague-Dawley rats were randomly divided into saline control group, HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progestogen with moxibustion at the same time. The Changes of nipple diameter and height were measured. The rats’ skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas, Danzhong (CV 17) and Ganshu (BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.RESULTS
The diameter and height of the nipples in model group were prominently bigger and higher than that in control group (P < 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group (P < 0.01), and there was no significant difference between moxibustion group and control group. Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong (CV 17) and Ganshu (BL 18) decreased prominently in model group (P < 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently (P < 0.05).CONCLUSION
Treatment with moxibustion can effectively decrease the HMG rats’ nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas, Danzhong (CV 17) and Ganshu (BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia. 相似文献16.
Zhang Lihong Zhuang Zhihong Sun Yanan Ma Shuhua Yang Weifeng Lei Hongtao Zuo Junling Ouyang Jingfeng Wang Yi 《中医杂志(英文版)》2017,37(3):308-313
Objective
To investigate the neural differentiation capacity of water extraction of velvet antler.Methods
Velvet antler (Cervus Nippon Temminck) polypeptide (VAP) was used to differentiate neural stem cells (NSCs) towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immunofluorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.Results
The differentiating cells look like neuron, some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron -like cells can express the special neural molecules.Conclusion
Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide. 相似文献17.
Yin Yukun Feng Li Zhou Lei Li Jie Gao Yin Wang Ningjun Yu Jianhua Jiang Zhenglong He Shengqi Lu Dianrong Wang Fang Du Yanlin Zhang Weiping 《中医杂志(英文版)》2017,37(1):30-34
OBJECTIVE
To investigate the effect of Yishengukang decoction on the expression of the metabolic bone markers, bone-specific alkaline phosphatase (BAP), carboxyterminal propeptide of type I procollagen (PICP), and carboxyterminal cross-linked telepeptide of type I collagen (ICTP), in cancer patients with bone metastasis.METHODS
Patients (n = 180) were divided into three groups: (a) bone metastasis patients treated with Yishengukang and pamidronate disodium injection (treatment group, n = 60); (b) bone metastasis patients treated with pamidronate disodium injection alone (control group, n = 60); (c) cancer patients without metastatic bone lesion (non-bone metastasis group, n = 60). Serum levels of the metabolic markers BAP, PICP, and ICTP were detected by enzyme-linked immunosorbent assay pre- and post-therapy.RESULTS
A significant decrease in serum BAP level was observed in the treatment group compared with the control group. However there were no significant differences in serum levels of PICP and ICTP before or after treatment compared with the control group.CONCLUSION
Yishengukang decoction combined with pamidronate disodium injection reduced serum BAP level to a greater extent that pamidronate disodium injection alone. Furthermore, the combined therapy was more beneficial in regulating imbalanced bone metabolism after bone metastasis, and may represent the molecular mechanism underpinning the effects of Yishengukang decoction. 相似文献18.
Objective
To investigate the effect of Sophora flavescens alkaloid (SFA) in gel form on aerobic vaginitis (AV) and the possible mechanism underlying the effects.Methods
AV rat models were prepared by intravaginal inoculation of Escherichia coli and Staphylococcus aureus. SFA gel and placebo gel were intravaginally administered. In vivo antibacterial effects, vaginal microenvironment, vaginal smears, pathological tissues of vaginas, and retention of gel in the vaginal cavity were investigated.Results
SFA gel had much higher antibacterial effect than placebo gel. SFA gel protected the vaginal mucosa from erosion of bacteria. At the same time, they inhibited the inflammatory responses, exhibiting little leukocytes and parabasal cells. Furthermore, the number of vaginal Lactobacilli remarkably increased following administration of SFA gel. However, the vaginal pH did not recover to the healthy acidic levels after treatment due to the buffering effect of gel. The gel of a fluorescent agent, Cyanine 7, showed very long retention time in the vaginal cavity, up to more than 24 h, much longer than the solutions.Conclusion
The SFA gel is a promising medicine for local treatment of AV with the advantages of anti-bacteria, protection of vaginal mucosa, increase of Lactobacilli, and long retention time in the vaginal cavity. 相似文献19.
Zhang Dan Wei Kai Ma Xiaopeng Wu Huangan Hong Jue Zhang Cuihong Wu Lingxiang Yan Huang Liu Jie Zhu Yi Yang Ling 《中医杂志(英文版)》2018,38(2):218-226
OBJECTIVE
To observe the effect of stimulating Qihai (CV 6) and bilateral Tianshu (ST 25) with herb-partitioned moxibustion (HPM) in rats with Crohn's disease (CD), and to investigate the possible anti-inflammatory mechanism of HPM.METHODS
Forty rats were randomly divided into four groups (n = 10 rats per group): normal control (NC), model control (MC), mesalamine (MES), and HPM. The CD rat model was established in the MC, MES, and HPM groups by administering a mixture of trinitrobenzenesulfonic acid and alcohol via enema. The HPM group received HPM on Qihai (CV 6) and bilateral Tianshu (ST 25), while the MES group received intragastric mesalamine. Colonic histomorphological scores, and serum concentrations of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were assessed to evaluate the effects of HPM on colonic reparation and anti-inflammation. The expressions of Toll-like receptor 4 (TLR-4), nuclear factor κB inhibitor α (IkB-α), IkB kinase α/β (Iκκα/β), and NF-κB p65 were further analyzed to investigate the regulatory effects of the interventions on the TLR4/NF-κB pathway.RESULTS
CD rats showed inflammatory colonic damage and increased serum concentrations of TNF-α and IL-1β. The expressions of TLR4, IKKα/β, and NF-κB p65 in the colons of CD rats were significantly increased compared with the NC group, while the expression of IκBα (a key negative regulator of NF-κB p65) was decreased. HPM significantly mitigated colonic damage and reduced the serum concentrations of TNF-α and IL-1β. HPM downregulated the expressions of TLR4, IKKα/β, and NF-κB p65 in the colon, and upregulated the expression of IκBα. The effects of HPM in CD rats were similar to those of mesalamine.CONCLUSION
HPM alleviates colonic inflammation in CD rats. This may be achieved through regulation of TLR4, which induces NF-κB signal transduction. 相似文献20.
Wang Xingze Chen Peijie Huang Xingyu Wang Yingying Yang Jinsheng Wichai Eungpinichpong Yang Yuming Uraiwan Chatchawan 《中医杂志(英文版)》2017,37(1):49-56