体外阻断GR功能对鸡脾淋巴细胞活性及Dex免疫抑制的影响

用地塞米松（Ｄｅｘ）和ＲＵ４８６分别或联合与鸡淋巴细胞，ＣｏｎＡ－一起培养，检测了淋巴细胞掺入＾３Ｈ－胸苷（＾Ｈ－ＴｄＲ）量的变化，１０＾－５和１０＾－６ｍｏｌ／ＬＲＵ４８６可抑制淋巴细胞掺入＾３Ｈ－ＴｄＲ（Ｐ〈０．０１），而１０＾－７ｍｏｌ／Ｌ则无明显影响（Ｐ〉０．０５）１０＾－５，１０＾－６和１０＾－７ｍｏｌ／ＬＤｅｘ地淋巴细胞掺入＾３Ｈ－ＴｄＲ有明显的抑制作用（Ｐ〈０．０１），１０＾－５ｍｏ     

降钙素基因相关肽抑制大鼠主动脉平滑肌细胞增殖

本工作用培养的大鼠主动脉平滑肌细胞，以细胞计数和＾３Ｈ－胸腺嘧啶掺入为指标，观察到降钙素基因相关肽呈剂量依赖地抑制ＶＳＭＣ增殖，１０＾－８和１０＾－７ｍｏｌ／ＬＣＧＲＰ可使＾３Ｈ－ＴｄＲ掺入量较对照组减少２６％（Ｐ＜０．０５）和３６％（Ｐ＜０．０１）并使细胞计数分别较对照组下降１７％（Ｐ＜０．０５）和３５％（Ｐ＜０．０１）。提示ＣＧＲＰ参与ＶＳＭＣ增殖的调节。     

全反式维甲酸对克隆化高转移人肺癌细胞浸润和转移的抑制作用

用５μｍｏｌ／Ｌ和１０μｍｏｌ／Ｌ全反式维甲酸（ＲＡ）处理克隆化高转移人肺癌细胞（ＰＧＣＬ３）５天后，细胞的体外生长速度、穿过Ｂｏｙｄｅｎ小室人工基底膜胶的浸润能力都受到一定的抑制，其中尤以１０μｍｏｌ／ＬＲＡ的作用明显。实验性转移显示，ＲＡ体外处理ＰＧＣＬ３细胞可在一定程度上降低其实验性转移的能力。此外通过ＤＮＡ－ＲＮＡ斑点杂交还发现ＰＧＣＬ３细胞经１０μｍｏｌ／ＬＲＡ处理５天后，人组织金属蛋白酶抑制剂基因（Ｔｉｍｐ－１和Ｔｉｍｐ－２）表达有一定水平的增高，这有助于进一步阐明ＲＡ抑制肿瘤细胞浸润和转移的机理。     

血管紧张素II对心肌梗塞大鼠成纤维细胞的核酸,胶原合成影响？…

目的和方法：本实验利用酶法分离、培养成年大鼠心肌成纤维细胞（Ｆｂｓ）,观察了血管紧张素Ⅱ（ＡｎｇⅡ）在不同处理因素下对Ｆｂｓ的＾３Ｈ－ＴｄＲ、＾１４Ｃ－ＵＲ、＾３Ｈ－Ｐｒｏ掺入率的影响。结果：在相同浓度（１０＾７ｍｏｌ／Ｌ）ＡｎｇⅡ作用下,ＭＩ组Ｆｂｓ对上述３种标记底物的掺入率均显著高于假手术（ＳＯ）组（Ｐ〈０．０５）。ＡｎｇⅡ的上述作用,可被特异性ＡｎｇⅡ拮抗剂＾［１．８］ＡｎｇⅡ和血管紧张素抗     

肽6A对球囊损伤后大鼠主动脉血管壁增殖的影响

本文在大鼠主动脉球囊内皮剥脱术后血管壁细胞过份增殖模型上，用合成的纤维蛋白（原）降解产物肽６Ａ（４０μｍｏｌ／ｋｇ／ｄ）治疗，可有效地抑制损伤血管壁的细胞计数增加和内膜增殖，显著降低其血管组织＾３Ｈ－ＴｄＲ和＾３Ｈ－Ｌｅｕ掺入增加程度，实验结果提示肽６Ａ作为血管成型术的辅佐剂，对于防治再狭窄可能具有潜在临床应用前景。     

生长抑素抑制内皮素刺激的家兔血管平滑肌细胞增殖

为探讨生长抑素（ＳＳＴ）对内皮素（ＥＴ）刺激细胞增殖的作用其机理，本工作在培养的兔主动脉血管平滑肌细胞（ＶＳＭＣ）上发现，１０＾－８ｍｏｌ／ＬＥＴ刺激细胞增殖（＾３Ｈ＝ＴｄＲ参入增多）和丝裂素活化蛋白激酶（ＭＡＰＫ）激活，１０＾－８ｍｏｌ／ＬＳＳＴ单独作用可抑制细胞增玩具增殖但不曩ＭＡＰＫ活性。ＳＳＴ不仅呈浓度依赖性地抑制ＥＴ刺激的ＶＳＭＣ增殖（Ｐ〈０．０１），而且亦抑制ＥＴ刺激的细胞ＭＡＰＫ激     

非胶原性间质成份对培养肝细胞DNA和胶原蛋白合成的影响

以透明质酸（ＨＡ）和层粘素（ＬＮ）为非胶原性间质成份的代表，观察其对大鼠培养肝实质细胞和人胎肝细胞＾３Ｈ－胸腺嘧啶核苷（＾３Ｈ－ＴｄＲ）和＾３Ｈ－脯氨酸（＾３Ｈ－Ｐｒｏ）掺入的影响。结果表明，正常血清浓度的ＨＡ和ＬＮ对肝细胞无明显的影响，高浓度则显著抑制＾３Ｈ－ＴｄＲ及＾３Ｈ－Ｐｒｏ的掺入，但抑制程度和剂量递增无显著相关性。     

反义Gαq／11亚基ODNs对平滑肌细胞DNA合成和PC蛋白表达的 …

目的：探讨Ｇｑ蛋白介导血管活性多肽对ＶＳＭＣＤＮＡ合成及增殖细胞核抗原（ＰＣＮＡ）蛋白表达的影响。方法：用硫代的Ｇαｑ／１１亚基反义寡聚脱氧核苷酸，以６μｍｏｌ／Ｌ的浓度加入含１０＾－７ｍｏｌ／Ｌ刺激无血清ＤＭＥＭ培养基中体外培养ＶＳＭＣ。用＾５Ｈ－ＴｄＲ掺入法测定ＶＳＭＣＤＮＡ合成，免疫细胞化学技术检测ＶＳＭＣＰＣＮＡ蛋白表达。结果：Ｇαｑ／１１ＡＳ－ＯＤＮｓ可明显抑制ＶＳＭＣＤＮＡＲ的合成，在     

人工细胞抗高血压因子对人脐静脉VSMC胞浆及核内Ca^2＋水平的影响

采实验用Ｆｌｕｏ－３／ＡＭ染色在激光扫描共聚焦显微镜下观察了红细胞抗高血压因子（ａｎｔｉｈｙｐｅｒｔｅｎｓｉｖｅ ｆａｃｔｏｒ,ＡＨＦ）对人脐静脉ＶＳＭＣ胞浆（〗Ｃａ＾２＋〖）及核内（〖Ｃａ＾２＋〗ｎ）游离钙离了瓣影响。结果表明：ＡＨＦ（１０＾－４ｇ／ｍＬ）明显抑制Ｂａｙ ｋ８６４４（１０＾－６ｍｏｌ／Ｌ）ＫＣｌ（６０ｍｍｏｌ／Ｌ）,ＡｎｇⅡ（１０＾－６ｍｏｌ／Ｌ）及ＩＰ３（１０＾－５ｍｏｌ／Ｌ）     

3H—TdR法分析小鼠增殖肝提取物活性

苯巴比妥钠诱导增殖的小鼠肝脏加热提取物５－２００ｍｇ／Ｌ，明显刺激人Ｌ０２肝细胞系＾３Ｈ－ＴｄＲ掺入，抑制５－Ｆｕ对人Ｌ０２肝细胞的杀伤作用；相同剂量的非加热提取物抑制人Ｌ０２肝细胞生长，苯巴比胺钠５－２０ｍｇ／Ｌ对人Ｌ０２细胞＾３Ｈ－ＴｄＲ掺入及５－Ｆｕ杀伤作用无影响，经胰蛋白酶预处理后，小鼠增殖肝加热提取物活性消失。这说明苯巴比妥钠本身对人Ｌ０２肝细胞不是一种丝裂原性药物，其诱导小鼠肝脏增殖作     

Photoperiodicity and the function of the hypophysis and the adrenal cortex

Summary The ACTH content of the hypophysis of rats rises from a minimum in the morning to a maximum in the evening hours. These fluctuations are associated with the diurnal rhythm of light and darkness, and are abolished under conditions of continual light or darkness for 30 days. Rhythmic fluctuations of the ascorbic acid content of the adrenals are also found, with maximum values in the evening, and these are likewise abolished by maintenance under conditions of constant illumination.Presented by Active Member AMN SSSR V. V. Parin     

Characteristics of the topographic interrelation of the head of the pancreas and the duodenum and pathology of the organs of the pancreatoduodenal area

Seventy pancreatoduodenal complexes of 55 patients with chronic pancreatitis and tumours of this zone and 15 patients died from other diseases are studies histotopographically . The pieces of the pancreatic head tissue in the medial wall of the duodenum were found in 12 cases of the first group and in 4 control cases. The pancreatic tissue consisted either of all elements of this organ or cystically dilated ducts and seemed to infiltrate different layers of the duodenum wall. Three variants of the pancreatic head structure are suggested on the basis of anatomo-topographical interrelationships of the pancreatic head and duodenum. In 12 out of 14 cases chronic pancreatitis and carcinoma of organs of this zone were combined with the variants of the pancreatic head structure, in 2 cases there was a true heterotopy . Pathogenetic significance of these variants for the development of chronic pancreatitis is discussed.     

指背腱膜滑动与近侧指间关节屈曲关系及临床意义

目的:探讨指背腱膜滑动距离与近侧指间关节(PIP)屈曲关系,为临床修复提供解剖学基础。方法:男性成人新鲜尸体标本10侧30指(示、中、环指各10指),切除手指皮肤,不破坏腱鞘、屈肌支持带、伸肌支持带、内在肌及外在肌,使肌腱保持正常的生理状态,分别测量各指中央束(CS)、侧束(LB)在PIP屈曲45°和90°时的滑动距离。结果:当PIP屈曲45°时,CS滑动距离为(2.7±0.4)mm,LB滑动距离为(2.8±0.6)mm;当PIP屈曲90°时,CS滑动距离为(4.3±0.7)mm,LB滑动距离为(4.8±0.6)mm。结论:指背腱膜滑动距离减少,严重影响手指的屈曲功能。对于指背腱膜的新鲜性损伤应予以精确修复;对于陈旧性损伤的修复应确保指背腱膜的正常滑动范围。     

产后妇女全血钙和骨源性碱性磷酸酶（ABAP）活性的检测与分析

目的结合我院特点,测定来我院进行产后检查妇女的骨源性碱性磷酸酶（ABAP）活性以及全血钙。方法采用金标法测定产妇3031人骨源性碱性磷酸酶（ABAP）的活性,并从中随机选择855人采用BH-5100型多通道原子吸收光谱仪检测全血钙。结果经测定,3031名产妇中679人骨源性碱性磷酸酶（ABAP）的活性异常,异常者检出率为22.4%,其中83.5%的产妇轻度缺钙,而16.5%的产妇严重缺钙;而全血钙异常检出率为17.0%。结论ABAP活性检测方法比全血钙检测方法测定体内钙营养水平更具敏感性,可作为检测评价产妇钙营养状况的一种简易可靠的方法,为产后骨质疏松提供了一种可靠、便捷、科学合理的指标。     

Cycloversion and cyclovergence: the effects of the area and position of the visual display

Rotation of a display in the frontal plane evokes a conjugate nystagmic rotation of the eyes (cycloversion) about the visual axes, with slow phases in the direction of stimulus motion — a response known as torsional optokinetic nystagmus (TOKN). Antiphase rotation of large dichoptic displays evokes a disconjugate rotation of the eyes about the visual axes, a response known as cyclovergence. Using the scleral-coil technique for monitoring eye movements we recorded TOKN evoked by black-and-white sectored displays rotating about the visual axis at an angular velocity of 30°/s. The display was confined to central areas with diameters ranging from 5° to full field or with the central 5° to 75° occluded. A 5° central display evoked TOKN with 40% of the gain for the full-field display and gain increased as a function of the size of the display. The gain of TOKN decreased with increasing size of a central occluder. These characteristics of TOKN are similar to those of horizontal OKN. Cyclovergence was virtually absent with a 5° display but was immune to occlusion of the central 40°. Cyclovergence therefore differs from cycloversion in showing no preference for centrally placed stimuli. These effects are free from the influence of stationary edges, since these were concentric with the stimulus motion. The effects are also free from the influence of voluntary pursuit, since humans do not normally have voluntary control over torsional eye movements.     

Organization and distribution of the upper and lower esophageal motoneurons in the medulla and the spinal cord of the rat

The upper cervical esophagus is exerted on swallowing and peristalsis by somatic and visceral motoneurons, whereas the lower esophagus is exerted on only peristalsis by visceral motoneurons. We examined the origin of the esophageal motoneurons and whether there were any differences between the distributions of the upper and the lower esophageal motoneurons in the medulla and the spinal cord using cholera toxin subunit b (CTb) as the retrograde tracer. Following injection of CTb into the cervical esophagus resulted in heavy labeling of the neurons in the nucleus ambiguus including the compact (AmC), semicompact (AmS) and loose (AmL) formations, and the medial column of lamina IX at the C1-C5 levels of the cervical spinal cord corresponding to the spinal accessory nucleus. A few labeled neurons were found in the inferior salivatory nucleus, the rostral division of the dorsal motor nucleus of the vagus (DMX), the accessory facial nucleus and the lateral column of lamina IX at the C2 and C3 levels. All these labeled neurons showed ChAT immunoreactivity. When CTb was injected into the cut end of the unilateral recurrent laryngeal nerve, many labeled neurons were found in the ipsilateral AmC, the AmL, and the bilateral medial column at the C1 and C2 levels. Following injection of CTb into the subdiaphragmatic esophagus resulted in heavy labeling of the neurons only in the AmC and the DMX. When CTb was injected into the sternomastoid muscle, many labeled neurons were found in the medullary reticular formation, the facial nucleus, the medial column at the C1-C3, C5 and C6 levels, and the lateral column at the C2, C3, C5 and C6 levels. Injections of a Fluoro-Gold into the cervical esophagus and a CTb into the sternomastoid muscle or the subdiaphragmatic esophagus in the same animal showed many double labeled neurons in the medial column of the accessory nucleus at the C1 and C2 levels, but no double labeled neurons in the AmC. These results indicated that the upper cervical esophagus is innervated by the visceral medullary vagal motoneurons as well as the somatic spinal accessory motoneurons. The lower esophagus is innervated only by the visceral medullary vagal motoneurons.