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1.
P. V. Sergeev A. S. Dukhanin F. R. Gubaeva 《Bulletin of experimental biology and medicine》1997,123(6):577-579
Changes in basal and stimulated levels of cAMP and calcium induced by hydrocortisone in a wide range of concentration (0.1–25
μM) are studies in a suspension of washed human platelets. The effects of hydrocortisone on the activity of preparations modulating
various stages of the adenylate cyclase system (forskolin, adenosine, adrenaline, and 3-isobutyl-1-methylxanthine) are compared.
Platelets are stimulated with collagen, platelet activating factor, and thapsigargin. Hydrocortisone in different concentrations
acts as both activator and inhibitor of calcium metabolism in platelets.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 6, pp. 663–665, June, 1997 相似文献
2.
S. B. Tkachenko V. N. Kokryakov I. P. Ashmarin S. V. Grachev A. A. Kubatiev 《Bulletin of experimental biology and medicine》1994,118(6):1291-1294
The effect of the total fraction of human defensins (HNP-1, HNP-2, and HNP-3) on the cytoplasmic Ca2+ content ([Ca2+]i) in the platelets of healthy donors was studied. At concentrations of 0.1–40 μg/ml and an incubation time of 10 min defensins
have no effect on [Ca2+]i in platelets labeled with Fura-2AM. However, at higher concentrations (100 μg/ml) they increased platelet [Ca2+]i. In addition, defensins (40 μg/ml) inhibited the Ca2+ increase in platelets induced by thrombin, adenosine diphosphate, and the lipopolysaccharide ofS. typhimurium endotoxin. The most pronounced inhibitory effect was observed in a suspension of thrombin-stimulated platelets. It is shown
that the effect of human defensins on the functional activity of platelets is due to the alterations in the intracellular
Ca2+.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
12, pp. 600–603, December, 1994 相似文献
3.
I. S. Severina 《Bulletin of experimental biology and medicine》1995,119(3):219-224
The articles is devoted to the role of heme in soluble guanylate cyclase functioning, the molecular mechanism of nitrogen
oxide activation of guanylate cyclase, the role of guanylate cyclase in the process of platelet aggregation, and the mechanism
of the antihypertensive and antiaggregative action of certain activators of the enzyme.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
3, pp. 230–235, March, 1995
Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences 相似文献
4.
T. V. Byzova T. N. Vlasik A. V. Mazurov 《Bulletin of experimental biology and medicine》1994,118(4):1102-1105
Monoclonal antibodies CRC64 are obtained against Ca2+-dependent glycoprotein IIb–IIIa complex of the platelet membrane which possess the ability to inhibit completely fibrinogen-dependent
platelet aggregation. CRC64 is directed against the epitope formed by the glycoprotein IIb–IIIa complex and does not interact
with proteins isolated after platelets are treated with ethylenediamine tetraacetate. Complete, reproducible blockade of platelet
aggregation caused by 5 μM adenosine diphosphate is noted in an MCA concentration of 3 μg/ml, while in the case of a stronger
inductor, namely 1 U/ml thrombin, platelet aggregation is inhibited in a concentration of 5 μg/ml. F(ab′)2 fragments are also able to inhibit platelet aggregation completely and are usually effective in concentrations lower than
native monoclonal antibodies.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
10, pp. 402–405, October, 1994
Presented by V. N. Smirnov, Member of the Russian Academy of Medical Sciences 相似文献
5.
Z. S. Smirnova Yu. V. Rodionova A. S. Khalanskii E. S. Gershtein G. K. Gerasimova 《Bulletin of experimental biology and medicine》1999,127(3):299-300
Potent therapeutic effect of hormonal cytostatic cortifen on intracranial brain tumors in rats was demonstrated. The effect
was seen after single cortifen course in glioblastoma expressing glucocorticoid receptors and after two courses in glucocorticoid
receptor-negative anaplastic astrocytoma. These findings confirmed the concept that selectivity of antitumor drugs can be
improved when hormones are used as the carries of cytotoxic groups.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 3, pp. 331–333, March, 1999 相似文献
6.
A. V. Zubkov T. L. Bushueva M. M. Minashkin Ya. G. Gurskii M. Yu. Men'shikov 《Bulletin of experimental biology and medicine》1999,128(3):952-955
Urokinase caused plasmin-dependent inhibition of platelet aggregation in platelet-rich plasma, while its proteolytically inactive
form had no such effect. Both forms potentiated the increase in platelet calcium concentration induced by aggregation inductors
and facilitated aggregation of washed platelets. In contrast to full-length urokinase molecule, its aminoterminal fragment
inhibited platelet aggregation and the corresponding elevation of intracellular calcium. These data suggest that urokinase
exerts a plasmin-independent effect on platelet activity. This effect depends on urokinase structure.
Translated formByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 9, pp. 339–343, September 1999 相似文献
7.
The effect of chlorophos (dipterex, trichlorphon) on high-threshold potassium and calcium currents is studied on isolated
snail neurons using the patch-clamp technique. Chlorophos (10–1000 μmol/liter) is found to reversibly lower the peak amplitude
of a high-threshold potassium current by 30% on average and exerts two independent effects on a high-threshold calcium current:
reversible lowering of the peak amplitude by 35% on average and, in 30% of cases, reversible inhibition of its activation,
inactivation, and deactivation. this effect is abolished by adding diltiazem (a calcium channel antagonist) in a concentration
of 100 μmol/liter to the medium.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
o
1, pp. 59–62, January, 1996
Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences 相似文献
8.
P. A. Galenko-Yaroshevskii A. I. Khankoeva A. S. Dukhanin D. S. Galygo 《Bulletin of experimental biology and medicine》1996,122(2):802-804
Isoproterenol and suphan, two cardioactive drugs with different mechanisms of action, are studiedin vitro for their effects on calcium homeostasis in myocardial cells. Isoproterenol lowers the basal Ca2+ level in resting cardiomyocytes and potentiates its rise in these cells after their induction. Suphan stimulates reversible
elevation of the diastolic Ca2+ concentration, causing increased calcium accumulation in the sarcoplasmic reticulum of cardiomyocytes. In anin vitro model of hypoxia, the Ca response to isoproterenol is significantly reduced, whereas that to dibutyryl cAMP is retained.
The effect of suphan on the Ca2+ content of cardiomyocytes exposed to “chemical” hypoxia is 30–50% higher than its effect on the Ca2+ content of intact cells.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 8 pp. 170–172, August, 1996 相似文献
9.
S. V. Shirshev Yu. I. Shilov N. N. Kevorkov 《Bulletin of experimental biology and medicine》1995,120(2):823-825
The ability of estradiol, progesterone, and chorionic gonadotropin to influence prostaglandin F2α production by intact splenocytes of CBA mice was studied. Estradiol and progesterone similarly activated the processes of
prostaglandin F2α production. No relationship was revealed between the effect and the concentration of the hormones. Chorionic gonadotropin
activated prostaglandin production by immunocompetent cells but only when used in a concentration reflecting the peak of its
physiological secretion. Combining gonadotropin with estradiol or progesterone did not lead to any appreciable differences
in the prostaglandin-stimulating action of each hormone alone.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
o
8, pp. 178–180, August, 1995
Presented by K. P. Kashkin, Member of the Russian Academy of Medical Sciences 相似文献
10.
Autooxidized high-density lipoproteins (HDL2) inhibit ADP-induced platelet aggregation in platelet-rich plasma. Platelet aggregation in the presence of native HDL2 and HDL3 and autooxidized HDL3 does not differ from the control (plasma with buffer). A conclusion is made on the important role of autooxidized HDL2 as a thrombogenesis-inhibiting factor in atherosclerosis.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 8, pp. 160–163, August, 1998 相似文献
11.
E. G. Fokina A. M. Polyakova O. S. Astrina V. V. Maleev 《Bulletin of experimental biology and medicine》1994,118(6):1315-1317
The effect of the toxic substances of diphtheria corynebacteria (diphtheria toxin, diphtheria anatoxin, and codivac) on the
aggregation of human plateletsin vitro was demonstrated using platelet-rich plasma prepared from citrated blood and the standard platelet activator ADP (2×10−5 M). These substances induce platelet aggregation in a dose-dependent manner. Incubation of diphtheria toxin and anatoxin
with platelets reduces ADP-induced and total platelet aggregation, the effect being dependent on the inducer dose and incubation
time. By contrast, codivac stimulates ADP-induced and total platelet aggregation in all experimental series.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
12, pp 623–625, December, 1994
Presented by V. I. Pokrovskii, Member of the Russian Academy of Medical Sciences 相似文献
12.
P. V. Sergeev P. A. Galenko-Yaroshevskii A. I. Khankoeva A. S. Dukhanin 《Bulletin of experimental biology and medicine》1996,121(3):265-267
The dynamics of the Ca-response of cardiomyocytes is studied and the efficiency of befol, verapamil, and amiodarone is compared
using various experimental models of stimulation of [Ca2+]i. Befol (1–5 μM) is shown to inhibit the caffeine-and strophanthin G-induced rise of [Ca2+]i. Unlike verapamil and amiodarone, befol exhibits no Ca-blocking activity in modeled K-depolarization. It is concluded that
the cardiotropic effect of befol is mediated through its primary action on Na+/Ca2+ exchange in cardiomyocytes, while the cardioplegic effect of verapamil and amiodarone is due to their ability to block the
slow Ca2+ inward current.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
o
3, pp. 288–291, March, 1996 相似文献
13.
P. V. Sergeev Yu. K. Napolov E. V. Markina E. N. Bolotova N. L. Shimanovskii 《Bulletin of experimental biology and medicine》1997,123(3):251-254
It is shown that Triombrast=Hexabrix=Omnipaque≤Melitrast<Ultravist in a dose-independent manner increase the level of prostaglandin
F2α and leukotrienes C4 and B4 in the blood of sensitive rats (50%) 15 min after intravenous injection of the preparation, the changes in prostaglandin
F2α being maximal, while those in leukotrienes C4 minimal. The effect of nonionic contrast agents on the blood level of prostaglandin F2α (0.1–2.0 g I/kg, except for Omnipaque) and leukotriene B4 (in a dose of 0.5 g I/kg) is more pronounced in comparison with the ionic preparations.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 3, pp. 289–293, March, 1997 相似文献
14.
Ya. Yu. Bagrov N. I. Dmitrieva N. B. Manusova 《Bulletin of experimental biology and medicine》1995,120(3):892-894
The effects of M1 and M2 cholinoceptors on stimulated water transport in the urinary bladder of the common frogRana temporaria L. are described. In the presence of pirenzepine, a selective M1 cholinoceptor antagonist, carbachol stimulated water transport. Activation of M2 cholinoceptors by oxotremorine in concentrations of 0.5–5.0 μM inhibited water transport, whereas their activation by this
compound in higher concentrations (10–100 μM) stimulated it. The use of the phospholipase C inhibitor neomycin (0.5 mM) and
the calmodulin inhibitor W-7 (1 mM) indicated that activation of M2 cholinoceptors switches on phospholipid-Ca2+-calmodulin-dependent mechanisms.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
o
9, pp. 252–254, September, 1995
(Presented by P. V. Sergeev, Member of the Russian Academy of Medical Sciences) 相似文献
15.
Antioxidant properties of thiamine 总被引:1,自引:0,他引:1
P. I. Lukienko N. G. Mel'nichenko I. V. Zverinskii S. V. Zabrodskaya 《Bulletin of experimental biology and medicine》2000,130(3):874-876
Thiamine (10−4–10−6 M) inhibits lipid peroxidation in rat liver microsome and free radical oxidation of oleic acidin vitro. Thiamine interacts with free radicals and hydroperoxides and is oxidized to thiochrome and thiamine disulfide. The antioxidant
effect of thiamine is probably related to sucessive transfer of 2H+ from the NH2 group of the pyrimidine ring and H+ from the thiazole ring (after its opening) to reactive substrates.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 303–305, September, 2000 相似文献
16.
Lukienko PI Mel'nichenko NG Zverinskii IV Zabrodskaya SV 《Bulletin of experimental biology and medicine》2000,130(9):874-876
Thiamine (10−4–10−6 M) inhibits lipid peroxidation in rat liver microsome and free radical oxidation of oleic acidin vitro. Thiamine interacts with free radicals and hydroperoxides and is oxidized to thiochrome and thiamine disulfide. The antioxidant
effect of thiamine is probably related to sucessive transfer of 2H+ from the NH2 group of the pyrimidine ring and H+ from the thiazole ring (after its opening) to reactive substrates.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 303–305, September, 2000 相似文献
17.
Electrophysiological properties of the inward rectification of neurons in the rat suprachiasmatic nucleus (SCN) were examined
by using the single-electrode voltage-clamp method, in vitro. Inward rectifier current (I
H) was produced by hyperpolarizing step command potentials to membrane potentials negative to approximately −60 mV in nominally
zero-Ca2+ Krebs solution containing tetrodotoxin (1 μM), tetraethylammonium (40 mM), Cd2+ (500 μM) and 4-aminopyridine (1 mM).I
H developed during the hyperpolarizing step command potential with a duration of up to 5 s showing no inactivation with time.I
H was selectively blocked by extracellular Cs+ (1 mM). The activation of the H-channel conductance (G
H) ranged between −55 and −120 mV. TheG
H was 80–150 pS (n=4) at the half-activation voltage of −84±7 mV (n=4). The reversal potential ofI
H obtained by instantaneous current voltage (I/V) relations was −41±6mV (n=4); it shifted to −51±8mV (n=3) in low-Na+ (20 mM) solution and to −24±4 mV (n=4) in high-K+ (20 mM) solution. Forskolin (1–10 μM) produced an inward current and increased the amplitude ofI
H. Forskolin did not change the half-activation voltage ofG
H. 8-Bromo-adenosine 3′,5′-cyclic monophosphate (8-Br-cAMP, 0.1–1 mM) and dibutyryl-cAMP (0.1–1 mM) enhancedI
H. 3-Isobutyl-1-methylxanthine (IBMX, 1 mM) also enhancedI
H. The results suggest that the inward rectifier cation current is regulated by the basal activity of adenylate cyclase in
neurons of the rat SCN. 相似文献
18.
Yu. V. Bukanova E. I. Solntseva V. G. Skrebitskii 《Bulletin of experimental biology and medicine》1998,126(4):1006-1009
A high-threshold (−20 mV) K+ current was recorded from isolated edible snail neurons by a two-microelectrode voltage clamp technique. This current consisted
of three components: fast-inactivating K+ currents (IA), noninactivating K+ current (IKD), and Ca2+-dependent K+ current (IK(Ca)). Different cells had one to three components of K+ current. Vinpocetine increased IA, moderately inhibited IKD (by 30–50%) and strongly suppressed IK(Ca) (by 60–90%). Inhibition of IK(Ca) was not related to the effect of vinpocetine on the inward Ca2+ current. When K+ current consisted of all three components, the effect of vinpocetine on the ionic current amplitude was opposite at different
potentials.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 10, pp. 408–411, October, 1998 相似文献
19.
A. I. Khankoeva P. A. Galenko-Yaroshevskii A. S. Dukhanin 《Bulletin of experimental biology and medicine》1996,122(1):689-691
Using benzofuran isophthalate, a fluorescent probe for sodium ions, intracellular (sarcoplasmic) Na+ concentrations ([Na+]i) were estimated in cardiomyocytes isolated from the left ventricle of rats. Lidocaine (1–100 μM) had little effect on [Na+]i in resting (unstimulated) cardiocytes, while befol lowered it by virtue of its inhibitory effect on Na/H exchange. In cardiomyocytes
exposed to “chemical” hypoxia (produced by 5 mM KCN+30 mM 2-deoxyglucoses). [Na+] were three times higher than in resting cells, and the Na-blocking effects of both lidocaine and befol were much stronger.
When these two drugs were used together, potentiation of these effects was observed, which may be accounted for by their action
on different Na-transporting systems.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 5, pp. 45–47, July, 1996 相似文献
20.
P. A. Galenko-Yaroshevskii A. I. Khankoeva A. S. Dukhanin 《Bulletin of experimental biology and medicine》1996,122(1):701-703
Effects of suphan, a new cardiotonic agent containing succinyl tryptophan, on the entry of Ca2+ into rat cardiomyocytes, its intracellular compartmentalization, and its exit from these cells were evaluatedin vitro. It was found that the recorded sulfan-induced rise of intracellular calcium was due to Ca2+ entering the cell via L-type calcium channels, and that a reversible reduction of its concentration in the sarcoplasm occurred
through its accumulation in the sarcoplasmic reticulum and was blocked by the specific Ca2+-ATPase inhibitor thapsigargin (10 μM). Suphan did not alter the activity of Na+/Ca2+ exchange in a concentration range of 5–150 μg/ml.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 7, pp. 57–59, July, 1996 相似文献