抗CD20 嵌合抗体突变型Fab′片断诱导凋亡过程中活性氧Caspase-3 的变化

 目的 研究抗CD20嵌合抗体突变型Fab’片断诱导Raji细胞凋亡过程中活性氧（ROS），Caspase-3的变化。方法 利用MTT法测定突变型Fab’片断抑制细胞生长，形态学方法观察凋亡细胞的变化，用流式细胞仪检测DCFH—DA荧光探针标记细胞内I的S的变化，以及用酶标仪和Westernblot检测细胞内Caspase-3的变化。结果 MTT法测定突变型Fab’片断对Raji细胞的生长具有抑制作用，其抑制作用呈明显的剂量依赖性，荧光显微镜下观察细胞出现凋亡，流式细胞仪，酶标仪以及Westernblot检测细胞内ROS，Caspase-3的表达增高，并与作用时间，剂量呈依赖关系。结论 ROS，Caspase-3表达的增高，在抗CD20嵌合抗体突变型Fab’片断诱导Raji细胞凋亡的过程中起到重要作用。     

抗CD20嵌合抗体突变型Fab’片断诱导凋亡过程中活性氧Caspase－3的变化

目的研究抗CD20嵌合抗体突变型Fab’片断诱导Raji细胞凋亡过程中活性氧（ROS），Caspase-3的变化。方法利用MTT法测定突变型Fab’片断抑制细胞生长，形态学方法观察凋亡细胞的变化，用流式细胞仪检测DCFH—DA荧光探针标记细胞内I的S的变化，以及用酶标仪和Westernblot检测细胞内Caspase-3的变化。结果MTT法测定突变型Fab’片断对Raji细胞的生长具有抑制作用，其抑制作用呈明显的剂量依赖性，荧光显微镜下观察细胞出现凋亡，流式细胞仪，酶标仪以及Westernblot检测细胞内ROS，Caspase-3的表达增高，并与作用时间，剂量呈依赖关系。结论ROS，Caspase-3表达的增高，在抗CD20嵌合抗体突变型Fab’片断诱导Raji细胞凋亡的过程中起到重要作用。     

嵌合抗CD20Fab′抗体片段诱导Raji细胞的凋亡机制

目的:探讨嵌合抗CD20抗体Fab′片段的抗肿瘤机制.方法:应用MTT法检测Fab′片段对Raji细胞生长的抑制作用,用Annexin V-FITC和PI测定Fab′片段对Raji细胞凋亡的诱导作用,用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化.结果:嵌合抗CD20Fab′片段对Raji细胞具有明显的抑制作用IC50值为24.2μg/ml,并呈剂量依赖性;嵌合抗CD20 Fab'片段能诱导Raji细胞的凋亡,并降低Raji细胞中bcl-2基因及其蛋白的表达水平.结论:嵌合抗CD20抗体片段Fab'能抑制Raji细胞生长和诱导细胞凋亡,其作用机制与bcl-2蛋白表达的下降有关.     

抗CD20嵌合抗体片段Fab′突变体对B淋巴细胞凋亡及胞内Ca~(2 )的影响

目的研究抗CD20嵌合抗体片段Fab′突变体诱导B淋巴细胞凋亡的发生、凋亡相关基因表达以及细胞内钙离子的浓度变化。方法以人B淋巴细胞(Raji细胞)为凋亡细胞模型,通过MTT法、Western blot和流式细胞仪等方法观察bcl-2/bax基因表达、细胞色素c及胞内Ca2 的变化。结果抗CD20嵌合抗体片段Fab′突变体对Raji细胞生长增殖具有明显的抑制作用,并呈剂量依赖性。细胞中bcl-2表达有微弱的下降,而bax表达明显升高,并出现细胞色素c的释放和胞内Ca2 浓度的升高。结论抗CD20嵌合抗体片段Fab′突变体诱导Raji细胞凋亡,与bcl-2/bax表达、细胞色素c的释放和胞内Ca2 浓度的升高有关。     

嵌合抗CD20Fab′抗体片段诱导Raji细胞的凋亡机制

目的: 探讨嵌合抗CD20抗体Fab′片段的抗肿瘤机制。方法: 应用MTT法检测Fab′片段对Raji细胞生长的抑制作用，用Annexin VFITC和PI测定Fab′片段对Raji细胞凋亡的诱导作用，用RTPCR和Western blot检测Raji细胞中bcl2表达的变化。结果: 嵌合抗CD20Fab′片段对Raji细胞具有明显的抑制作用IC50值为24.2 μg/ml，并呈剂量依赖性；嵌合抗CD20 Fab′片段能诱导Raji细胞的凋亡，并降低Raji细胞中bcl2基因及其蛋白的表达水平。 结论:嵌合抗CD20抗体片段Fab′能抑制Raji细胞生长和诱导细胞凋亡，其作用机制与bcl2蛋白表达的下降有关     

抗CD20嵌合抗体片段Fab''突变体对B淋巴细胞凋亡及胞内Ca2+的影响

目的：研究抗CD20嵌合抗体片段Fab’突变体诱导B淋巴细胞凋亡的发生、凋亡相关基因表达以及细胞内钙离子的浓度变化。方法：以人B淋巴细胞（Raji细胞）为凋亡细胞模型，通过MTT法、Westernblot和流式细胞仪等方法观察bcl-2／bax基因表达、细胞色素c及胞内Ca^2＋的变化。结果：抗CD20嵌合抗体片段Fab’突变体对Raji细胞生长增殖具有明显的抑制作用，并呈剂量依赖性。细胞中bcl-2表达有微弱的下降，而bax表达明显升高，并出现细胞色素c的释放和胞内Ca^2＋浓度的升高。结论：抗CD20嵌合抗体片段Fab’突变体诱导Raji细胞凋亡，与bcl2／bax表达、细胞色素c的释放和胞内Ca^2＋浓度的升高有关。     

嵌合抗CD20 Fab‘对B淋巴瘤细胞Raji细胞生长的影响

目的：研究嵌合抗CD20基因工程抗体Fab‘的抗肿瘤活性。方法：利用MTT法以及^3H掺入法测定嵌合抗CD20 Fab‘对Raji细胞生长的影响。结果：MTT法测定结果显示嵌合抗CD20 Fab‘对Raji细胞的生长具有抑制作用，抑制作用成剂量依赖性，其IC50为24ug/ml；嵌合抗CD20 Fab‘对^3H-TdR掺入Raji细胞无抑制作用，表明抗CD20 Fab‘不影响Raji细胞DNA的合成；但嵌合抗CD20 Fab‘抑制^3H-UdR掺入Raji细胞，表明嵌合抗CD20 Fab‘对Raji细胞RNA合成具有抑制作用，其抑制作用成剂量相关性。结论：嵌合抗CD20 Fab‘抑制Raji细胞生长，嵌合抗CD20 Fab‘具有较好的抗肿瘤活性。     

单价抗CD20抗体诱导人B细胞淋巴瘤Raji细胞的凋亡

背景与目的:抗CD20抗体和片段已应用于非霍奇金淋巴瘤的临床治疗,但仍需要开发新的抗CD20抗体和片段(未修饰的或放射性标记的),以治疗对美罗华(利妥昔单抗)无反应的患者。鼠源性抗CD20抗体HI47的嵌合抗体片段Fab和F(ab)'2已被构建。本研究目的是观察HI47(鼠抗-CD20抗体)和其嵌合抗CD20抗体片段抑制肿瘤细胞生长和诱导肿瘤细胞凋亡的作用。方法:用免疫荧光法测定抗CD20抗体与CD20+人B细胞淋巴瘤Raji细胞的结合能力;MTT法测定抗CD20抗体片段对Raji细胞生长的影响;用膜联蛋白Ⅴ染色和DNA琼脂糖凝胶电泳检测和验证抗CD20抗体片段诱导Raji细胞凋亡。结果:HI47和其嵌合的抗CD20抗体片段均可与CD20+Raji细胞结合,结合率可达90%以上;HI47不能与美罗华竞争结合Raji细胞;HI47和其嵌合的抗CD20抗体片段浓度为100μg/ml对Raji细胞的抑制率分别为:(57.0±1.5)%、(65.2±2.5)%、(77.2±3.2)%;单价的抗CD20抗体片段Fab(20μg/ml)能够诱导Raji细胞的凋亡,早期凋亡率为17%。结论:HI47的嵌合抗体片段对Raji细胞有抑制作用,能诱导Raji细胞的凋亡。     

尼美舒利对人食管癌Eca-109细胞凋亡及Survivin和Caspase-3表达的影响

目的:观察环氧化酶-2(COX-2)选择性抑制剂尼美舒利对人食管癌Eca-109细胞增殖及凋亡的影响,研究其对凋亡抑制蛋白Survivin和Caspase-3表达的影响,探讨尼美舒利诱导Eca-109细胞凋亡的作用机制.方法:尼美舒利作用Eca-109细胞后,MTT法测定尼美舒利对人食管癌Eca-109细胞增殖的抑制率;电子显微镜和流式细胞仪检测细胞凋亡:RT-PCR法检测Eca-109细胞Survivin mRNA表达变化,Western blot检测Survivin和Caspase-3蛋白表达变化.结果:尼美舒利(50～400μmol/L)对Eca-109细胞生长有抑制作用,随浓度升高、时间延长抑制作用增强,并诱导Eca-109细胞凋亡,呈剂量-时间效应关系;尼美舒利可降低Survivin mRNA和蛋白表达,增加Caspase-3蛋白表达.结论:尼美舒利可诱导人食管癌细胞株Eca-109凋亡,其机制可能与下调Survivin表达及激活Caspase-3表达有关.     

嵌合抗CD20 Fab′对B淋巴瘤细胞Raji细胞生长的影响

目的：研究嵌合抗CD_(20)基因工程抗体Fab′的抗肿瘤活性。方法：利用MTT法以及3H掺入法测定嵌合抗CD_(20) Fab′对Raji细胞生长的影响。结果：MTT法测定结果显示嵌合抗CD_(20) Fab′对Raji细胞的生长具有抑制作用，抑制作用成剂量依赖性，其IC50为24μg／ml；嵌合抗CD_(20) Fab′对3H－TdR掺入Raji细胞无抑制作用，表明抗CD_(20) Fab′不影响Raji细胞DNA的合成；但嵌合抗CD_(20) Fab′抑制3H－UdR掺入Raji细胞，表明嵌合抗CD_(20) Fab′对Raji细胞RNA合成具有抑制作用，其抑制作用成剂量相关性。结论：嵌合抗CD_(20) Fab′抑制Raji细胞生长，嵌合抗CD_(20) Fab′具有较好的抗肿瘤活性。     

Ringworm in animals in a farm in Assiut

Bericht über das Auftreten von Hautmykosen bei Tieren auf der Farm der Landwirtschaftlichen Fakultät der Universität Assiut. Von 70 Kälbern waren 42 pilzkrank, davon 22 durch T. mentagrophytes, 20 durch T. verrucosum. Von 180 Kühen waren 8 pilzbefalien, sämtlich durch T. mentagrophytes. Von 2 kranken Bullen wurde T. verrucosum isoliert. Auch die 3 Pferde und 2 Maultiere der Farm waren pilzinfiziert; Erreger war in diesen Fällen T. equinum. Auch ein Kalb war von T. equinum befallen. Die gleiche Pilzart wurde ferner von 3 Tierpflegern isoliert, die Pilzherde am Hals und an den Armen aufwiesen.     

Statistical challenges in preprocessing in microarray experiments in cancer

Many clinical studies incorporate genomic experiments to investigate the potential associations between high-dimensional molecular data and clinical outcome. A critical first step in the statistical analyses of these experiments is that the molecular data are preprocessed. This article provides an overview of preprocessing methods, including summary algorithms and quality control metrics for microarrays. Some of the ramifications and effects that preprocessing methods have on the statistical results are illustrated. The discussions are centered around a microarray experiment based on lung cancer tumor samples with survival as the clinical outcome of interest. The procedures that are presented focus on the array platform used in this study. However, many of these issues are more general and are applicable to other instruments for genome-wide investigation. The discussions here will provide insight into the statistical challenges in preprocessing microarrays used in clinical studies of cancer. These challenges should not be viewed as inconsequential nuisances but rather as important issues that need to be addressed so that informed conclusions can be drawn.     

Trends in zygomycosis in children

Zygomycosis, or mucormycosis, is associated with significant morbidity and mortality in both children and adults. Studies in adults have shown an increase in the incidence of zygomycosis, particularly among haemtopoietic stem cell transplant (HSCT) recipients and patients with haematologic malignancies. There is a paucity of data on the epidemiology of zygomycosis in children. We performed a retrospective analysis to describe trends in zygomycosis between 1 January 2003 and 31 December 2010. We used the Pediatric Health Information System (PHIS) database to identify paediatric patients who were diagnosed with zygomycosis during the study period. Administrative data on diagnoses, demographics, underlying conditions and clinical experiences were collected. Summary statistics were calculated and tests for trend were conducted. We identified 156 unique patients with zygomycosis. The prevalence of zygomycosis did not significantly increase over time (P=0.284). The most common underlying condition was malignancy (58%) and over half received intensive care. Voriconazole utilisation among all hospitalised children significantly increased during the period (P=0.010). Our study demonstrates that the incidence of zygomycosis is not significantly increasing. During the time period there was a significant increase in the use of voriconazole among children.     

Hemoglobin in lymphogranulomatosis in children

The peculiarities of hemoglobin content and properties in lymphogranulomatosis were studied in children by different methods. In most patients the content of fetal hemoglobin and methhemoglobin was found to increase and the rate of ferricyanide oxidation of hemoglobin also increases. Simultaneously, the rate of non-homogenicity of basic hemoglobin fractions was observed to increase on account of immunologically different components.     

Dermatomycosis in Goats in India

Summary: In an extensive survey involving 2176 goats 1.56% of goats manifested clinical lesions of ringworm infection. Animals below the age of 6 months were affected most (4.20%). The incidence of infection was higher during the winter months. T. verrucosum, T. mentagrophytes and M. gypseum were isolated from the skin scrapings of 12, 4, and 2 goats respectively. It is suggested that the infected animals could be a source of disease to human population in which the zoophilic dermatophyte invasion is characterized by severe inflammatory lesions of the skin. The zoonotic importance of different dermatophytes is stressed.
Zusammenfassung: In einer ausgedehnten Untersuchung an 2176 Ziegen wurden bei 1,56% klinische Zeichen einer Hautmykose festgestellt. Tiere, die jühger als 6 Monate alt waren, zeigten mit 4,20% am häfigsten Krankheitssymptome. In den Wintermonaten war die Erkrankungshäufigkeit am gröBten. T. vermcosum, T. mentagrophytes and M. gypseum wurden jeweils von 12,4 bzw. 2 Ziegen isoliert. Die inflzierten Tiere köinnen eine Infektionsquelle für die menschliche Bevölkerung darstellen und dort Mykosen mit stark entzündlichen Veränderungen auslösen. Die Bedeutung verschiedener Dermatophyten als Erreger von Zoonosen wird hervorgehoben.     

Sex hormones in women in rural China and in Britain

Plasma concentrations of certain hormones linked to breast cancer risk were measured in age-pooled samples from 3,250 rural Chinese women in 65 counties, and 300 British women, all aged 35-64. In age-groups 35-44, 45-54 and 55-64 respectively, mean oestradiol concentrations were 36% (P = 0.043), 90% (P less than 0.001) and 171% (P = 0.001) higher in the British than in the Chinese women, and mean testosterone concentrations were 48% (P less than 0.001), 68% (P less than 0.001) and 53% (P = 0.001) higher in the British than in the Chinese women. The difference in testosterone concentrations between the two countries appeared to be due largely to the lower average body weight in the Chinese women. Sex hormone binding globulin did not differ significantly between the two countries in age groups 35-44 and 45-54, but was 15% (P = 0.002) lower in the British than in the Chinese women at ages 55-64. Prolactin concentrations did not differ significantly between the two countries in any age group.