过敏原检测在儿科的临床应用

过敏原检测在儿科临床方面有着非常重要的应用,由于绝大多数儿童年幼无知并且好动、好奇心强,接触一些容易引起过敏的物体,时常发生过敏现象。本文主要介绍了过敏原检测方法在儿科临床应用时的方法选择以及具有的现实意义。     

B淋巴细胞亚群在儿童过敏原免疫治疗中的作用

目的 通过比较儿童过敏原免疫治疗前后B淋巴细胞亚群的变化,明确B细胞亚群在过敏原免疫治疗(AIT)治疗机制中的作用.方法 使用流式细胞计数法检测32例儿童过敏原免疫治疗前后1年B淋巴细胞亚群的数量,另选取32例年龄性别匹配的健康儿童作为对照组.结果 AIT治疗后活性B淋巴细胞的相对数和绝对数均明显低于治疗前水平(P =0.001),其余B淋巴细胞亚群治疗前后差异无统计学意义(P＞0.05).结论 过敏原免疫治疗直接影响B淋巴细胞细胞分化,导致活性B细胞计数减少.活性B细胞计数或可作为AIT治疗效果及是否需要继续治疗的评估指标,具有重要的临床应用价值.     

过敏原检测在儿科中的临床应用

目的探讨过敏原检测在儿科临床上的应用价值。方法选取我院在2009年7月至2012年3月间收治的145例儿科患者,对所有患儿的临床资料及实际情况进行分析,并对患儿采用过敏原进行检测,评估患儿的检测结果。结果所有患儿均明确了容易导致疾病的过敏原,有效减少了疾病治疗过程中的影响因素。结论对儿科患者实行过敏原检测可以有效明确患儿的过敏原,并给予其对症治疗,因此值得在临床上推广应用。     

过敏原检测在哮喘诊断及防治中的意义

目的探讨定量过敏原检测在哮喘诊断及防治中的意义。方法采用allergyscreen过敏源诊断试剂盒检测83例哮喘患儿血清过敏原特异性IgE抗体和总IgE抗体。结果本组患儿总IgE抗体阳性率61.5%,不同过敏原特异性IgE抗体以户尘螨粉尘螨阳性率(71.2%)最高。结论户尘螨粉尘螨是3岁以上小儿哮喘发作的主要诱因。支气管哮喘患儿总IgE水平增高。     

过敏原检测在儿童哮喘中的应用及临床意义

目的:探讨皮肤过敏以及血清原检测在儿童哮喘中的应用及临床诊断的意义。方法:选择2011年1月—2013年1月就诊的儿童哮喘患者80例,随机分为观察组和实验组,各40例。实验组患者应用粉尘螨以及屋尘螨进行皮肤点刺试验检测,观察组患者应用免疫条带印记法进行血清过敏原检测。另外选取40例健康儿童作为对照组,进行点刺试验。比较分析三组过敏原检测结果。结果:实验组粉尘螨和屋尘螨阳性率明显高于对照组,差异有统计学意义(P<0.05);观察组中血清过敏原呈阳性者32例(80.00%),其中狗毛29例(72.50%),猫毛30例(75.00%),海鲜组合25例(62.50%),粉尘螨30例(75.00%),屋尘螨31例(77.50%)。皮肤点刺试验与血清过敏原检测的尘螨过敏原检出率比较,差异无统计学意义(P>0.05)。结论:皮肤点刺试验用于儿童哮喘病过敏原检测能取得满意的效果,可以作为最佳的检测方法。     

小儿支气管哮喘特异性免疫治疗及亚高原地区常见吸入过敏原的调查研究

目的探讨特异性免疫治疗 (脱敏治疗 )小儿哮喘的疗效。了解亚高原地区常见吸入过敏原与小儿哮喘的关系。方法 116例小儿哮喘随机抽出 78例 ,作过敏原检测 ,以阳性的 6 2例为治疗组 ,剩余的 38例及阴性的 16例作对照组 ,治疗组采用常规哮喘治疗加脱敏治疗 ,对照组仅作常规治疗。测第一秒时间肺活量 (FEV1 0 )判断疗效 ,并对该地区常见吸入过敏原与小儿哮喘的关系进行总结。结果治疗组总有效率为 98 38% ,对照组为 85 19% ,X =5 36 ,P <0 0 5 ,差异有显著意义 ;测FEV1 0 ,经比较 ,治疗前t=1 5 45 ,差异无显著意义 ,治疗后比较t=4 6 3,P <0 0 1,差异有极显著意义 ;治疗组复发 2例 ,复发率 3 2 3% ,对照组复发 2 1例 ,复发率 38 88% ;18种过敏原检测阳性率为79 4% ,以尘螨最高为 76 9%。结论 (1)脱敏治疗小儿哮喘疗效肯定 ,复发少 ;(2 )亚高原地区小儿哮喘过敏原检测阳性率高 ,以尘螨最为常见。     

生物共振诊断治疗仪对常见过敏性疾病过敏原检测及疗效观察

目的 评价生物共振诊断治疗仪对慢性过敏性疾病过敏原检测的准确性和治疗效果.方法 采用生物共振诊断治疗仪对慢性过敏性疾病患者进行过敏原检测,并对其中部分同意治疗的患者进行治疗.结果 本组2400例,共检测出常见过敏原25种.1094例采用生物共振诊断治疗仪进行治疗,痊愈202例,显效252例,有效431例,总有效率80.9%.结论 生物共振诊断治疗仪在诊治慢性过敏性疾病方面优势明显,且检测、治疗过程安全、无痛苦,不良反应少.     

生物共振诊断治疗仪对常见过敏性疾病过敏原检测及疗效观察

目的 评价生物共振诊断治疗仪对慢性过敏性疾病过敏原检测的准确性和治疗效果.方法 采用生物共振诊断治疗仪对慢性过敏性疾病患者进行过敏原检测,并对其中部分同意治疗的患者进行治疗.结果 本组2400例,共检测出常见过敏原25种.1094例采用生物共振诊断治疗仪进行治疗,痊愈202例,显效252例,有效431例,总有效率80.9%.结论 生物共振诊断治疗仪在诊治慢性过敏性疾病方面优势明显,且检测、治疗过程安全、无痛苦,不良反应少.     

生物共振诊断治疗仪对常见过敏性疾病过敏原检测及疗效观察

目的 评价生物共振诊断治疗仪对慢性过敏性疾病过敏原检测的准确性和治疗效果.方法 采用生物共振诊断治疗仪对慢性过敏性疾病患者进行过敏原检测,并对其中部分同意治疗的患者进行治疗.结果 本组2400例,共检测出常见过敏原25种.1094例采用生物共振诊断治疗仪进行治疗,痊愈202例,显效252例,有效431例,总有效率80....     

尘螨过敏原与过敏性疾病

尘螨是一类主要存在于室内尘土中的螨类,分布广泛,由于它们体积很小,如果不用显微镜就无法观察到它们。尘螨是最主要的室内吸入性过敏原之一,在临床上可引起人体许多过敏反应如过敏性哮喘、过敏性鼻炎、过敏性皮炎等。因此,尘螨越来越受到人们的关注。     

RNA干扰在心血管疾病研究中的现状和展望

RNA干扰可特异性使目的基因沉默,具有高效、特异和低毒的特点,正逐渐成为研究心血管疾病发病机制和防治措施的重要工具。本文就RAN干扰的研究简史、作用机制、在心血管疾病细胞水平和整体动物水平的研究、RNA干扰的局限性及改善策略和在心血管疾病研究中的展望作一综述,为心血管疾病的防治提供新思路。     

Allergen immunotherapy: an effective immune-modifier

Allergen-specific immunotherapy (IT) consists in administering gradually increasing doses of an allergen extract to sensitive patients. This practice results in ameliorating symptoms associated with the subsequent exposure to the causative allergen. Presently, the lack of therapies which affect the pathogenesis of the disease make IT the only treatment that may improve the natural course of allergic diseases.     

Small-molecule CRTH2 antagonists for the treatment of allergic inflammation: an overview

The discovery of a second receptor for prostaglandin D₂, chemoattractant receptor-homologous molecule expressed on T helper type 2 (CRTH2), has revived significant efforts into the development of small-molecule antagonists for allergic diseases as the receptor is predominantly expressed on cells such as eosinophils, T_H2 cells and basophils, which are major pro-inflammatory cells in diseases such as asthma and allergic rhinitis. This brief review serves to illustrate current patent literature focusing on the similarities and differences of an ever-growing number of CRTH2 antagonists emerging from corporate laboratories.     

Small-molecule CRTH2 antagonists for the treatment of allergic inflammation: an overview

The discovery of a second receptor for prostaglandin D2, chemoattractant receptor-homologous molecule expressed on T helper type 2 (CRTH2), has revived significant efforts into the development of small-molecule antagonists for allergic diseases as the receptor is predominantly expressed on cells such as eosinophils, TH2 cells and basophils, which are major pro-inflammatory cells in diseases such as asthma and allergic rhinitis. This brief review serves to illustrate current patent literature focusing on the similarities and differences of an ever-growing number of CRTH2 antagonists emerging from corporate laboratories.     

Neurotuberculosis: an overview

Although pulmonary tuberculosis is the most common form of this disease, neurotuberculosis is more severe and presents higher morbidity and mortality. Its diagnosis continues to challenge physicians all over the world. Contributing to this fact is the nonspecificity of its clinical manifestations, the low density of bacilli in the cerebrospinal fluid (CSF), and the delayed recovery of Mycobacterium tuberculosis through culture techniques. Thus, the diagnosis is largely based on suspicious symptoms, and the prognosis is directly related to the stage of the disease at the beginning of treatment. Even thought there is no consensus regarding the best therapeutic regimen, the WHO recommends using the same regimen used for pulmonary tuberculosis with a longer treatment time. It is important to note that in most cases, the doctor will not have a definite diagnosis at the beginning of the treatment. However, this should not delay the initiation of therapy. A delay in initiating treatment, in most cases, is directly associated with a poor prognosis. This review gives an overview of the current state of the neurotuberculosis research. It covers the epidemiological aspects of the infection, pathogenesis, principal clinical presentations, diagnosis highlighting neuroimaging, where a series of imaging are presented, prognosis, prevention and therapeutic regimens.     

Cubosomes: an overview

Cubosomes are nanoparticles but instead of the solid particles, cubosomes are self-assembled liquid crystalline particles of certain surfactant with proper ratio of water with a microstructure that provides unique properties of practical interest. The discovery of cubosomes is a unique story and spans the field of food science, differential geometry, biological membranes and digestive processes. One of the most common surfactants used to make cubosomes is the monoglyceride glycerol monoolein. Bicontinuous cubic liquid crystalline phase is an optically clear, very viscous material that has a unique structure at the nanometer scale. The word bicontinuous refers to the division of the two continuous but non-intersecting aqueous regions by a lipid bilayer that is contorted into a space-filling structure. Hydrating a surfactant or polar lipid that forms cubic phase and then dispersing the solid-like phase into smaller particles usually form Cubosomes. There is a lot of excitement about the cubic phases because its unique microstructure is biologically compatible and capable of controlled release of solubilized active ingredients like drugs and proteins.