慢性胃炎脾胃虚实证红细胞游离Ca^2＋及膜Ca^2＋—Mg^2＋— …

目的：从细胞和细胞膜酶分子水平探讨慢性胃炎中医脾胃虚实证病理生理特征。方法：测定１０２例慢性胃炎患者和３０例正常人红细胞［Ｃａ＾２＋］ｉ、ＡＴＰ含量、膜Ｃａ＾２＋－Ｍｇ＾２＋－ＡＴＰａｓｅ活性和２４ｈ尿１７－羟皮质类固醇排出量。结果：湿热证细胞内外Ｃａ＾２＋转换率加快,膜Ｃａ＾２＋－Ｍｇ＾２＋－ＡＴＰａｓｅ活性和细胞内ＡＴＰ合成量呈代偿性亢进;脾胃气虚Ｃａ＾２＋转换率有所下降,膜Ｃａ＾２＋－Ｍｇ＾     

血压持续偏高青少年红细胞内离子浓度及膜ATP酶活性的变化观察

我们测定了３８例血压持续偏高青少年红细胞膜ＡＴＰ酶活性和红细胞内阳离子浓度。结果表明：Ｎａ＾＋－Ｋ＾＋－ＡＴＰａｓｅ和Ｃａ＾２＋－Ｍｇ＾２＋－ＡＴＰａｓｅ活性明显低于血压正常青少年。红细胞钠、钙含量，红细胞内游离钙水平均明显高于血压正常者。提示：青少年期血压持续偏高个体已存在细胞内钙、钙离子水平的改变，Ｎａ＾＋０Ｋ＾＋－ＡＴＰａｓｅ和Ｃａ＾２＋－Ｍｇ＾２＋ＡＴＰａｓｅ活性降低是血压持续偏高的重要环     

急性心肌梗死患者红细胞的变形能力与其三磷酸腺苷酶活性关系的研究

本文观察了５４例急性心肌梗死（ＡＭＩ）患者发病３天内红细胞变形能力（ＥＤ）和红细胞三磷酸腺苷酶（ＡＴＰａｓｅ）活性及红细胞内离子浓度的变化，并分析其相互关系。结果显示，ＡＭＩ患者红细胞滤过指数（ＩＦ）校对照组明显增高（Ｐ＜０．００１）。红细胞Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－Ｍｇ２＋－ＡＴＰａｓｅ活性和红细胞Ｋ＋、Ｍｇ２＋浓度明显降低，而红细胞Ｎａ＋、Ｃａ２＋浓度明显增高，与对照组相比差异有显著性意义（Ｐ＜０．００１）。ＡＭＩ患者红细胞ＩＦ与红细胞Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－Ｍｇ２＋－ＡＴＰａｓｅ活性、红细胞Ｋ＋、Ｍｇ２＋浓度呈负相关（Ｐ＜０．００１），与红细胞Ｎａ＋、Ｃａ２＋浓度呈正相关（Ｐ＜０．００１）。这提示ＡＭＩ患者ＥＤ降低与红细胞ＡＴＰａｓｅ活性和Ｋ＋、Ｍｇ２＋浓度降低及Ｃａ２＋、Ｎａ＋浓度增高有关。     

糖尿病患者自由基与红细胞膜Na~ -K~ -ATPase、Ca~(2 )-ATPase 损伤的关系

糖尿病患者自由基与红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－ＡＴＰａｓｅ损伤的关系严孙杰卓孝福吴长珠王中心潘时中胡雯刘友尧赵淑好红细胞功能和结构异常是糖尿病慢性并发症产生的重要因素，其中红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－ＡＴＰａｓｅ活...     

钙,硒对膳食低钙大鼠红细胞钠／钾ATP酶及钙／镁ATP酶活性的影响

实验通过向模拟克山病偏食饲料中补充一定量的钙和硒／维生素Ｅ（Ｓｅ／ＶＥ）来观察大鼠红细胞钠／钾ＡＴＰ酶及钙／镁ＡＴＰ酶（Ｎａ－Ｋ－ＡＴＰａｓｅ,Ｃａ－Ｍｇ－ＡＴＰａｓｅ）活性的变化。探讨钙和硒对大鼠ＲＢＣ膜的变化规律。结果表明,病区粮组血钙水平最低,仅及常规食（ｓｔｏｃｋ）组一半,血清硒（Ｓｅ）,α－ＶＥ水平低于ｓｔｏｃｋ组,红细胞Ｎａ－Ｋ－ＡＴＰａｓｅ,Ｃａ－Ｍｇ－ＡＴＰａｓｅ,活性降低,血清过     

实验性动脉粥样硬化兔血小板胞浆游离钙和钙泵的变化

实验观察了食饵性兔动脉粥样硬化形成过程中血小板胞浆游离钙［Ｃａ＾２＋］ｉ、血清钙、红细胞膜钙泵即Ｃａ＾２－Ｍｇ＾２＋－ＡＴＰａｓｅ的活性，主动脉收缩性的变化，与以照组比较结果表明：（１）高脂兔血小板［Ｃａ＾２＋］ｉ水平增高，红细胞膜Ｃａ＾２＋－Ｍｇ＾２＋－ＡＴＰａｓｅ活性及主动脉收缩性降低；（２）主动脉组织光镜可见高脂兔主动脉中层有大量钙沉积；（３）扫描电镜可见血小板粘附于内皮细胞表面。     

原发性高血压患者红细胞内CA^2＋,Mg^2＋水平及细胞膜ATP酶活性

对正常人及原发性高血压患者细胞内、外Ｃａ２＋、Ｍｇ２＋浓度和细胞膜ＡＴＰ酶活性之间的关系进行评价。方法检测３２名正常人（ＮＴ），５５例原发性高血压病（ＨＴ）患者血浆和细胞内Ｃａ２＋、Ｍｇ２＋浓度（原子吸收法），细胞膜ＡＴＰ酶活性（比色法）。结果ＨＴ组血浆Ｃａ２＋浓度明显低于ＮＴ组，红细胞内Ｃａ２＋浓度明显高于ＮＴ组，红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶及Ｃａ２＋－ＡＴＰ酶活性明显低于ＮＴ组。血浆Ｍｇ２＋和红细胞内Ｍｇ２＋浓度与ＮＴ组无明显差别。结论原发性高血压患者细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性明显减低，血浆Ｃａ２＋水平明显减低，红细胞内Ｃａ２＋超载。反映膜缺陷和离子运输系统失常与原发性高血压发病有关。而细胞内、外Ｍｇ２＋水平变化与高血压之间的关系须进一步研究。     

COPD肺动脉高压患者红细胞膜Ca~(2 )-Mg~(2 )-ATP酶活性观察

ＣＯＰＤ肺动脉高压患者红细胞膜Ｃａ２＋－Ｍｇ２＋－ＡＴＰ酶活性观察李为民陈文彬吴兆锋左凤琼慢性阻塞性肺病（ＣＯＰＤ）继发肺动脉高压的发病机制，目前尚未完全阐明。现已明确，血管平滑肌细胞内游离Ｃａ２＋的增高，在低氧性肺血管收缩中起着重要的作用，而Ｃａ２...     

心肌缺血再灌注损伤肌浆网Ca^2＋—ATPase活性变化电镜酶细胞化学观察

心肌缺血再灌注损伤肌浆网Ｃａ２＋－ＡＴＰａｓｅ活性变化电镜酶细胞化学观察（摘要）谷天祥肖德绵张显清谷春久石玉秀李厚文姜桂娥王天骄应用电镜酶细胞化学技术观测大鼠离体缺血再灌注心肌细胞肌浆网、细胞膜原位Ｃａ２＋－ＡＴＰａｓｅ活性变化，旨在探讨心肌缺血再灌...     

钙调素与肺动脉高压

ＣａＭ是细胞内的主要钙结合蛋白质。Ｃａ＾２＋与ＣａＭ结合形成Ｃａ＾２＋－ＣａＭ复合物，调节腺苷酸环化酶、磷酸二酯酶、Ｃａ＾２＋－Ｍｇ＾２＋ＡＴＰ酶及肌球蛋白轻链激酶的活性，并影响细胞内游离Ｃａ＾２＋浓度，参与低氧性肺血管收缩。     

Hp相关性慢性胃炎中医证型分布及血清胃泌素-17水平的相关性分析

目的:探讨Hp相关性慢性胃炎中医证型分布及各证型间胃泌素-17(G-17)水平的相关性.方法:收集Hp相关性慢性胃炎患者400例,按慢性胃炎中医辨证分为脾胃湿热证、脾胃虚寒证、寒热错杂证、肝胃不和证、胃阴不足证、胃络瘀阻证6个证型,并测各证型内患者G-17水平.结果:Hp相关性慢性胃炎各证型例数由高到低分别为:脾胃湿热...     

隆起糜烂性胃炎证型与幽门螺杆菌感染及p53和增殖细胞核抗原表达的相关性

[目的]通过观察隆起糜烂性胃炎(Raised Erosive Gastritis,REG)中医证型与幽门螺杆菌(Hp)感染和p53、增殖细胞核抗原(PCNA)表达之间的关系,以期更好的协助指导中西医结合防治REG。[方法]选择REG中医辨证符合脾胃湿热证、脾胃气虚证、脾虚湿热证的患者63例,并设正常对照(正常)组18例,所有受试者均行胃镜检查,取胃窦部活检标本行快速尿素酶试验及组织染色法检测Hp及p53、PCNA。[结果]REG组各证型的p53、PCNA表达均较正常组增加(均P0.01);各证型的p53、PCNA阳性表达分别比较差异均无统计学意义(均P0.05);各证型Hp阳性与Hp阴性者p53、PCNA阳性表达比较差异均无统计学意义(均P0.05)。[结论]REG胃黏膜处于高增殖状态。REG中医各证型间与Hp感染及p53、PCNA表达无明显差异。     

非酒精性脂肪性肝病常见中医证候类型及证候要素的现代文献研究

目的:探讨非酒精性脂肪性肝病(NAFLD)常见中医证候类型与证候要素的分布特点.方法:通过检索文献,选取1979年-2011年有关NAFLD中医辨证的文献,建立数据库,进行统计分析.结果:从纳入分析的文献中共获取168个证候类型,规范为53个,出现频率＞5％的证候依次为:肝胆湿热、肝郁脾虚、肝气郁结、肝肾阴虚、脾胃虚弱、肝都痰阻.提取证候要素共计18个,其中病位类8个,频率＞5％的依次为肝、脾、胆、胃、肾;病性类10个,频率＞5％的依次为湿、热(或火)、气滞、痰、虚、血瘀.结论:目前NAFLD临床辨证分型复杂多样,而证候要素相对简约,从构成证候的要素入手对本病进行常见证候的研究,可起到执简驭繁的作用,并为临床治疗本病奠定理论基础.     

Platelet and erythrocyte Mg2+, Ca2+, Na+, K+ and cell membrane adenosine triphosphatase activity in essential hypertension in blacks.

OBJECTIVE: To assess the relationship between intracellular Mg2+, Ca2+, Na+ and K+ and cell membrane adenosine triphosphatase (ATPase) activity in normotensive and hypertensive blacks. DESIGN: Intracellular cations and cell membrane ATPase activity were studied in black patients with untreated essential hypertension and age-, weight- and height-matched normotensive controls. Platelet, erythrocyte and serum Mg2+, Ca2+, Na+ and K+ levels as well as platelet and erythrocyte membrane Na+,K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase activities were measured in all subjects. METHODS: Intracellular Na+ and K+ were measured by flame photometry and Mg+ and Ca+ by atomic absorption spectrophotometry. Cell membrane ATPase activity was determined by a colorimetric method. RESULTS: The hypertensive group consistently demonstrated depressed activity of each ATPase studied, with significantly lower serum Mg2+, serum K+, erythrocyte Mg2+ and platelet Mg2+ levels compared with the normotensive group. Platelet Na+ and Ca2+ and erythrocyte Ca2+ were significantly elevated in the hypertensive group. In the hypertensive group, mean arterial pressure (MAP) was inversely correlated with platelet and erythrocyte membrane Na+,K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase. Serum Mg2+, serum Ca2+ and platelet Mg2+ were negatively correlated with MAP in the hypertensive group whilst erythrocyte and platelet Ca2+ were positively correlated. In the normotensive group, platelet Mg2+ and MAP were negatively, and erythrocyte Ca2+ and MAP, positively correlated. CONCLUSIONS: Black patients with essential hypertension have widespread depression of cell membrane Na+,K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase activities with serum and intracellular Mg2+ depletion and cytosolic Na+ and Ca2+ overload, which may reflect an underlying membrane abnormality in essential hypertension. These cellular abnormalities may be related to the defective transport mechanisms that in turn may be aggravated by Mg2+ depletion.     

幽门螺杆菌相关性慢性胃炎中医辨证分型与胃泌素的关系

目的:研究幽门螺杆菌(Hp)相关性慢性胃炎中医辨证分型与空腹血清胃泌素(GAS)的关系.方法:将中医辨证为脾胃湿热型、脾胃虚弱型的42例慢性胃炎患者进行电子胃镜、病理组织学检查,并检测Hp及血清GAS.结果:Hp相关性慢性胃炎中脾胃湿热型占62.96%(P<0.05);空腹血清GAS(125.35 ng/L)高于脾胃虚弱型(96.59 ng/L,P<0.01).结论:高GAS血症可能是Hp相关性慢性胃炎脾胃湿热证型重要病理生理基础之一.     

594例轻度慢性乙型肝炎中医证候分布规律调查研究

目的:探讨轻度慢性乙型肝炎(CHB)患者的中医证候分布规律,为规范化治疗CHB提供参考依据.方法:运用中医证候量表对594例轻度CHB患者进行中医证候调查,采用聚类分析的方法对纳入的患者进行聚类,并结合变量频数分析对证型归属进行判定.结果:收集的轻度CHB病例均以肝郁脾虚为主要证型,在此基础上合并有6个兼证,分别为肝郁脾虚兼湿热阴虚血瘀证型(62.80％)、肝郁脾虚兼肝肾阴虚证型(24.24％)、肝郁脾虚兼肝郁化火证型(5.05％)、肝郁脾虚兼肝胃不和证型(3.70％)、肝郁脾虚兼中气亏虚证型(2.53％)和肝郁脾虚兼瘀血阻络证型(1.68％).肝郁脾虚兼湿热阴虚血瘀证型又进一步分为5个亚型,分别为脾虚为主型(45.30％)、普通型(22.52％)、气滞为主型(17.70％)、湿热为主型(10.46％)、肾虚为主型(4.02％).结论:肝都脾虚证型为轻度CHB的基本证型.     

脾虚证183例分级与木糖及胰功肽试验的相关性研究

目的研究脾虚证分级量化与木糖及胰功肽试验的相关性及科学依据。方法内镜及活检确诊的浅表性胃炎115例,萎缩性胃炎15例,消化性溃疡19例及慢性结肠炎34例,把慢性胃肠病分为6型。即脾胃虚弱证(包括虚寒)、肝胃不和证、脾胃(大肠)湿热证、脾胃阴虚证、瘀血阻络证和脾肾阳虚证。进行证候分级量化,用木糖、胰功肽试验等指标同步检测。结果研究发现慢性胃肠病患者木糖、胰功肽含量均明显低于正常组(P<0.05-0.01),中医证型除脾胃(大肠)湿热证的木糖排泄率接近正常人外,其它证型的木糖、胰功肽水平均明显低于正常人(P<0.05-0.01)。对脾虚证及肝胃不和证进行分级量化,首次发现脾胃虚弱(寒)证随着症状分级的加重,木糖排泄率逐渐下降(P<0.01),肝胃不和证随着症状分级的加重,胰功肽都逐渐下降(P<0.001)。这为探讨证型的病理生理提供了可信的科学依据。结论木糖的变化规律较好地反映脾虚证的特异性,胰功肽的变化规律较好地反映肝胃不和证的特异性。     

小儿慢性胃炎辨证分型与内镜象及幽门螺杆菌感染的相关性研究

[目的]探讨小儿慢性胃炎中医证型与胃黏膜改变及幽门螺杆菌感染之间的内在关系.[方法]将符合入选条件的404例慢性胃炎患儿中医辨证分型后进行胃镜检查及尿素13C-呼气试验(13 C-UBT)检查,并登记造表,进行统计学处理.[结果]肝胃不和型胃炎主要表现为胃黏膜充血和微小结节形成,脾胃湿热型胃炎表现以黏膜斑和黏膜糜烂为主,胃络瘀血型胃炎则以胃黏膜出现出血斑点为主,脾胃虚弱型胃炎以胃黏膜水肿为主,胃阴不足型胃炎以胃黏膜花斑为主.13 C-UBT阳性率实证高于虚证,与证型无相关性;13C-UBT值实证高于虚证,尤以肝胃不和型和脾胃湿热型为最高.[结论]小儿慢性胃炎中医辨证分型与胃黏膜改变及幽门螺杆菌感染之间存在实质性联系,为辨证施治提供了理论基础.     

慢性病毒性肝炎279例病理诊断与中医证型的关系

目的:探讨慢性病毒性肝炎病理组织学诊断与中医辨证分型之间的关系,为中医辨证提供客观依据。方法:279例均为住院患者,男227例,女52例。全部病例经肝活检证实,其中轻度142例,中度91例,重度46例。慢性乙型肝炎274例,慢性丙型肝炎5例。中医辨证:湿热中阻53例,肝郁脾虚64例,肝肾阴虚29例,脾肾阳虚7例,血瘀血热101例,气阴两虚25例。临床治疗以辨证施治为主。结果:(1)279例慢性肝炎在病理诊断分型中,轻度占50.9%、中度占32.6％、重度占16.4％。(2)在轻度慢性肝炎中,湿热中阻、肝郁脾虚及肝肾阴虚证明显高于血瘀血热证.P=0.01、P<0.05。(3)湿热中阻、肝郁脾虚证的病理诊断分布情况依次为轻度>中度>重度,P<0.01、P<0.05;血瘀血热证轻度与中度分别>重度,P<0.05。但该证在重度中的发生率高于其他证型。(4)脾肾阳虚与气阴两虚证在各病理分型中的分布均无显著差异,P>0.05。结论:(1)慢性肝炎患者以轻度居多,占半数以上。(2)慢性肝病的病变早期以湿热中阻、肝郁脾虚为主证特点,病变部位多在气分;随着肝脏病理损害加重,病变部位及主导证型渐由气分至血分,以血瘀血热为主证特点。(3)治疗上主张早期宜注重清解湿热,疏肝健脾;中、晚期宜重视活血化瘀,凉血解毒。     

Ca2+-transport ATPases of vascular smooth muscle

To characterize the Ca2+-transport properties of the plasma membrane and of the endoplasmic reticulum of bovine pulmonary artery, membrane vesicles are subfractionated by a procedure of density-gradient centrifugation that takes advantage of the selective effect of digitonin on the density of plasma-membrane vesicles. The obtained endoplasmic-reticulum fraction contains hardly any plasma-membrane vesicles, whereas the plasma-membrane fraction is still contaminated by a substantial amount of endoplasmic-reticulum vesicles. An adenosine 5'-triphosphate (ATP) energized Ca2+-transport system and a Ca2+-stimulated ATPase activity are present in both subcellular fractions. The Ca2+ transport by the plasma membrane is catalyzed by a (Ca2+,Mg2+)-ATPase of Mr 130,000. It binds calmodulin and it has a low steady-state phosphoprotein intermediate level. The endoplasmic-reticulum vesicles contain a Ca2+-transport ATPase of Mr 100,000 that is characterized by a high steady-state phosphointermediate level. It is antigenically related to the Ca2+-pump protein of cardiac sarcoplasmic reticulum. Phospholamban, the regulatory protein of the Ca2+-transport enzyme of cardiac sarcoplasmic reticulum, is also present in the endoplasmic reticulum of the pulmonary artery. A comparison of these fractions with the previously characterized fractions from porcine gastric smooth muscle reveals important differences in the basal Mg2-ATPase activity, in the ratio of the (Ca2+,Mg2+)-ATPase of the plasmalemma to that of the endoplasmic reticulum, and in the ratio of the (Na+,K+)-ATPase activity to the plasmalemmal (Ca2+,Mg2+)-ATPase activity. These differences can be ascribed in part to the species and in part to the tissue. These data suggest that in the bovine pulmonary artery the Ca2+ extrusion via the ATP-dependent Ca2+ pump may have a less predominant role, and that the Ca2+ uptake by the endoplasmic reticulum, and possibly also the Ca2+ extrusion via the Na+-Ca2+ exchanger could be more important in this tissue than in the porcine stomach.