高效液相色谱法测定当归中阿魏酸的含量

目的:探讨高效液相色谱法测定当归中阿魏酸含量的方法.方法:以1%冰醋酸溶液为阿魏酸对照品的溶剂,40%甲醇溶液为样品溶剂,固定相为 Batesil C18(250 mm×4.6 mm,5 μm);流动相为甲醇∶1%冰醋酸∶甲氢呋喃(30∶70∶1);检测波长320 nm.结果:阿魏酸在3.05～30.5 μg/ml 范围内线性良好(r=0.9999,n=5),平均加样回收率为98.8%,RDS 为1.07%(n=9).结论:高效液相色谱法可用于当归药材中阿魏酸含量的测定.本方法简单准确,灵敏度高.     

高效液相色谱法测定保肝丸中五味子醇甲含量研究

目的:建立高效液相色谱法,对保肝丸中五味子醇甲的含量进行测定,为今后的药物研究工作提供可靠的参考依据。方法:采取反相高效液相色谱法对保肝丸中五味子醇甲含量进行检测,色谱柱选用Hanbon Lichrospher C18,规格为250 mm×4.6 mm×5μm,流动相为甲醇∶水=63∶37,检测波长采取250 nm,进样量为10μl。结果:经上述条件测定五味子醇甲在0.105～2.101μg浓度范围内具有良好的线性关系,r=0.998,加样回收率为(98.35±13.20)%,RSD为1.01%。结论:采取高效液相色谱法对保肝丸中五味子醇甲进行检测时具有操作简单、准确以及重复性好等优势,值得关注。     

RP-HPLC法测定鼻炎片中五味子醇甲的含量

目的 建立高效液相色谱法测定鼻炎片中五味子醇甲含量的方法.方法 色谱柱为迪马DiamonsiLC18柱(250 mm×4.0 mm,5μm);流动相为甲醇-水(体积比55:45);流速为1.0 mL/min;检测波长为250 nm.结果 五味子醇甲在0.08-2.56 μg范围内呈良好的线性关系,r=0.999 9,平...     

宁心安神口服液中酸枣仁皂苷A和B的含量测定

目的:建立宁心安神口服液中酸枣仁皂苷A和B含量的测定方法,保证药品质量和临床疗效.方法:应用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD),钻石ODS(250 mm×4.6 mm,5μm)色谱柱,甲醇-水-冰醋酸(70:30:1)为流动相,流速是1.0 mL/min;柱温:35℃,漂移管温度85℃,气体流速1.1 L/min.结果:酸枣仁皂苷A线性范围是2.4675μg～12.3375μg,r=0.99997、酸枣仁皂苷B线性范围是1.34μg～6.70μg,r=0.99991,酸枣仁皂苷A及B的平均回收率分别为99.31%(RSD=1.87%)和99.27%(RSD=1.51%)(n=6).结论:方法快速简便,重现性良好,可用于测定宁心安神口服液中酸枣仁皂苷A和B的含量.     

高效液相色谱法测定香附丸中芍药苷的含量

目的用高效液相色谱法测定香附丸中芍药苷的含量。方法采用高效液相色谱法,ZORBAX SB-C18分析柱(250mm×4.6mm,5μm);流动相:甲醇-0.05mol.L-1磷酸二氢钾溶液-三乙胺(28:72:0.5);检测波长:230nm;流速:1.0m l.min-1,柱温:40℃。结果芍药苷在0.44~3.96μg范围内的线性关系良好(r=0.9999),平均加样回收率为98.93%(n=5),重现性试验RSD为0.70%(n=5)。结论方法准确,灵敏度高,重现性好。     

HPLC法测定沈阳红药胶囊中人参皂苷Rg_1的含量

目的建立沈阳红药胶囊中人参皂苷Rg1含量测定的高效液相色谱方法。方法色谱柱:Agilent C18(250mm×4.6 mm,5μm);流动相:A为乙腈,B为水;梯度洗脱,流速:1.0 ml·min~(-1);柱温:30℃;检测波长:203nm。结果人参皂苷Rg_1在质量浓度在80.16-400.8μg·m L~(-1)范围内呈良好的线性关系(r=0.9996),加样回收率为96.72%,RSD为0.61%(n=6)。结论该方法定量准确可靠,操作简便,灵敏度高,能够使用于沈阳红药胶囊的质量控制。关键词:高效液相色谱法;人参皂苷Rg;沈阳红药胶囊     

高效液相色谱法测定祛瘀散结胶囊中人参皂苷Rg1的含量

[目的]建立测定祛瘀散结胶囊中人参皂苷Rg 1含量的方法.[方法]采用高效液相色谱法,所用色谱柱为Thermo色谱柱(4.6 mm×250.0 mm,5μm),流动相为乙腈-水(19∶81),流速为1 mL/min,检测波长为203 nm.[结果]人参皂苷Rg 1的进样量在0.325~5.200μg范围内时与峰面积积分值呈良好的线性关系,Y=369.089X+15.813,r=0.999 8(n=5),加样平均回收率为99.77%,RSD为1.57%.[结论]采用高效液相色谱法测定祛瘀散结胶囊中人参皂苷Rg 1含量的方法具有简便、快速、灵敏及重现性好等优点,可作为祛瘀散结胶囊质量控制方法.     

HPLC法测定降酶胶囊中五味子甲素的含量

目的:研究降酶胶囊中五味子甲素含量的测定方法。方法:采用高效液相色谱法。色谱柱C18(4.6mm×250mm,5μm);流动相为甲醇;流速1.0ml.min-1;柱温40℃:检测波长250nm。结果:五味子甲素线性范围是0.043～0.431,r=0.9999,平均回收率99.5%,RSD=0.67%(n=6)。结论:方法可用于测定降酶胶囊中五味子甲素的含量。     

参血胶囊中人参皂苷Rb1、Re、Rg1含量的测定

[目的]建立高效液相色谱法测定参血胶囊中人参皂苷Rb1、Re、Rg1含量测定方法。[方法]采用HP1100高效液相色谱仪(包括G1314A可变波长紫外检测器,HP化学工作站,G1322A真空脱气机,HP1100四元泵),ZORBAX SB-C18柱(4.6mm×250mm,5μm)。人参皂苷Rb1流动相:乙腈-水(30∶70);人参皂苷Re、Rg1流动相:乙腈-水(20∶80)。紫外检测波长:203nm,流速:1.0ml/min,进样量10μl。[结果]人参皂苷Rb1在0.752μg～9.4μg范围内呈现良好的线性关系,平均回收率为101.2%(n=6)。人参皂苷Rg1在0.408μg～3.4μg范围内呈现良好的线性关系,平均回收率为99.2%(n=6)人参皂苷Re在0.576μg～3.6μg范围内呈现良好的线性关系,平均回收率为99.7%(n=6)。人参皂苷Rb1、Rg1和Re日内精密度(n=6)RSD分别为0.92%、0.60%及0.78%;人参皂苷Rb1、Rg1和Re日间精密度(n=5)RSD分别为1.20%、0.96%及1.27%。[结论]该法简便、准确、具有专属性,可用于测定参血胶囊中人参皂苷Rb1、Re、Rg1的含量。     

高效液相色谱法测定芪白平肺胶囊中人参皂苷Rg1与Rb1含量

目的采用高效液相色谱法测定芪白平肺胶囊中人参皂苷Rg1与Rb1的含量,为其质量控制提供依据。方法采用Welch Material Inc C18色谱柱(4.6mm×250mm,5μm)。其流动相:水-乙腈;梯度洗脱(0~10min,81∶19;11~35min,81∶19→71∶29;36~50min,71∶29→50∶50);流速:1.2ml/min;检测波长:203nm;柱温:30℃。结果人参皂苷Rg1、Rb1在0.16~2.40μg范围内与其峰面积呈线性关系,r=0.999 9(n=8);人参皂苷Rg1、Rb1的平均加样回收率分别为102.31%、98.55%(n=9);芪白平肺胶囊中人参皂苷Rg1、Rb1的3批样品测定结果,人参皂苷Rg1平均含量分别为0.332、0.326、0.336mg/g,人参皂苷Rb1平均含量分别为0.496、0.509、0.511mg/g。结论所建立的高效液相色谱方法简便、灵敏度高、重现性好,可用于芪白平肺胶囊中人参皂苷的含量测定。     

PAROXYSMAL HEMOGLOBINURIA WITH A REPORT OF A CASE IN A CHINESE

Paroxysmal hemoglobinuria is an uncommon disease: Up to date there have been hardly 300 cases reported in the literature. The disease has appeared to have been relatively more common in Japan than elsewhere in this part of the world. So far, there is no record of its occurrence among the.Chinese. S. Inamori(11 and N. Ito (2) report- ed two cases from Mukden Medical College Hospital in Manchuria, but the nationality of these two patients was not mentioned.     

A Recurrent Ectopic Pregnancy within A Previous Caesarean Scar: A Case Report

Objective To investigate the clinical diagnosis and treatment of caesarean scar pregnancy (CSP). Methods We reported here a case of recurrent ectopic pregnancy within a previous cesarean scar and reviewed the literature. Results Surgical evacuation of a CSP was a small side effect and effective treatment of CSP. Conclusion Early and accurate diagnosis, timeliness and effective treatment were extremely important in saving patients?life and retaining their fertility.     

CHOLECYSTECTOMY THROUGH A MINI-LAPAROTOMY A PRELIMINARY REPORT

Cholecystectomy through a small subcostal incision (mini-lap cholecystectomy), has recently been introduced as an alternative to conventional cholecystectomy in an effort to reduce its attendant morbidity. A trial was conducted to assess the morbidity of cholecystectomy performed through a small subcostal incision. Eighteen consecutive patients posted for elective cholecystectomy were operated through such an incision. In 2 [11%], the incision had to be extended. The records of these patients were retrospectively compared with an equal number of consecutive cholecystectomies previously performed by the same surgeon through a conventional incision. There was no significant difference in the average operating time, incidence of wound infection or the number of post-operative complications between the conventional and the mini-laparotomy group. However, the number of doses of post operative analgesic required, the duration of post-operative ileus, hospitalisation and convalescence needed was nearly halved. Thus mini-lap cholecystectomy has much lesser morbidity and is considered to be a safe and viable alternative to conventional cholecystectomy.KEY WORDS: Cholecystectomy, Mini-laparotomy, Post-operative morbidity     

大胆假设,小心求证——培养研究生的几点体会

研究生培养应该是全方位的,通过学习,使综合素质得到提高,为将来的可持续发展打下一坚实的基础,其中能力的培养尤为重要.     

维生素A中毒1例

患儿 ,女 ,3岁 ,因“发热、头痛、呕吐伴咳嗽 2ｄ”入院 ,近 1个月来患儿全身皮肤反复出现皮疹 ,瘙痒明显 ,纳差。查体 :生命体征平稳 ,精神较差 ,全身皮肤粗糙 ,可见散在红色丘疹及新旧抓痕和血迹 ,口唇红 ,略干 ,咽部充血 ,双侧扁桃体ＩＩ度肿大 ,未见脓性分泌物 ,心、肺、腹部及神经系统检查无异常 ,拟诊“上呼吸道感染”予抗感染及对症治疗 ,患儿上呼吸道症状消失 ,而头痛、呕吐、皮肤瘙痒未见好转 ,并出现全身非凹陷性肿胀 ,以颜面、四肢为主 ,触痛明显 ,且口唇干燥、皲裂和鼻粘膜出血 ,怀疑维生素Ａ中毒。经详细追问病史 ,发现患儿自 …