氧氟沙星阴道泡腾片抗菌活性实验动物的研究

采用氧氟沙星（ＯＦＬＸ）阴道泡腾片和诺氟沙星（ＮＦＬＸ）阴道栓对临床常见的细菌性阴道炎进行了动物体内外抗菌活性测定。结果显示：体外测定组：ＯＦＬＸ对致病菌的ＭＩＣ在０．１￣３．１２μｇ／ｍｌ而ＮＦＬＸ ＭＩＣ与其相近。体内测定组ＯＦＬＸ的治愈率２ｍｇ／ｋｇ组为５０％，４ｍｇ／ｋｇ和８ｍｇ／ｋｇｘ组为１００％。而ＮＦＬＸ栓２ｍｇ／ｋｇ组为４０％，４ｍｇ／ｋｇ组为８０％，８ｍｇ／ｋｇ且为１００％，空白     

氧氟沙星阴道泡腾片抗菌活性实验动物的研究

采用氧氟沙星（ＯＦＬＸ）阴道泡腾片和诺氟沙星（ＮＦＬＸ）阴道栓对临床常见的细菌性阴道炎进行了动物体内外抗菌活性测定。结果显示：体外测定组：ＯＦＬＸ对致病菌的ＭＩＣ在０１～３１２μｇ／ｍｌ而ＮＦＬＸＭＩＣ与其相近。体内测定组：ＯＦＬＸ的治愈率２ｍｇ／ｋｇ组为５０％，４ｍｇ／ｋｇ和８ｍｇ／ｋｇ组为１００％。而ＮＦＬＸ栓２ｍｇ／ｋｇ组为４０％，４ｍｇ／ｋｇ组为８０％，８ｍｇ／ｋｇ组为１００％，空白组治愈率为０。说明ＯＦＬＸ阴道泡腾片对此类阴道炎具良好的疗效，低、中剂量组略优于ＮＦＬＸ栓。但两药无统计学差异     

地塞米松对内毒素损伤ET—1mRNA表达的影响

目的；观察地塞米松对内纱血症引起肺组织ＥＴ－１ｍＲＮＡ表达的影响，方法：选用ＳＤ大鼠２１只，等分为三组：生理盐水组（Ｃ组）；内毒素组（Ｌ组）ＬＰＳ５ｍｇ／ｋｇ静注；地塞米松组，静注ＬＰＳ５ｍｇ／ｋｇ前１ｈ先给地塞米松（Ｄ组）３ｍｇ／ｋｇ静注动物观察５ｈ处于进行研究。用ＲＴ－ＰＣＲ法测定肺组织ＥＴ－１ｍＲＮＡ的变化。结果：Ｌ组肺组织ＥＴ－１ｍＲＮＡ表达比Ｃ组但无统计学差异，Ｄ组肺组织病理损害明显减轻     

环磷酰胺对早期小鼠S180"治愈性"化疗评价

目的：探讨环磷酰胺（ＣＴＸ）对早期小鼠Ｓ１８０最佳治疗方案,为“治愈性”化疗科学依据。方法：六组Ｓ１８０荷瘤小鼠中,五组用ＣＴＸ腹腔内注射治疗,一组用生理盐水对照。治疗后８０天处死全部小鼠并经病理证明无瘤,评价为治愈。结果：在每天ＣＴＸ２０ｍｇ／ｋｇ和１００ｍｇ／ｋｇ１次组中,治愈率分别 烽５０％;肿瘤生长死亡率分别为１０％和２０％;毒性死亡率分别０％和３０％。在每天１次ＣＴＸ４０ｍｇ／ｋｇ胜利腔     

以环磷酰胺静脉冲击为主治疗小儿难治性肾病

采用环磷酰胺（ＣＴＸ）治疗难治性肾病５８例并随机分组观察。治疗组ＣＴＸ１０ｍｇ／（ｋｇ．ｄ）,静点冲击,６～８个疗程,对照组ＣＴＸ３ｍｇ／（ｋｇ．ｄ）口服,疗程３个月。结果示大剂量ＣＴＸＵ肝解率８７．５％。口服ＣＴＸ缓解率５５．６％（Ｐ〈０．０５）。且前者副作用发生率低,从而认为,大剂量ＣＴＸ冲击治疗难治性肾病是安全有效的。     

泼尼松加间断环磷酰胺冲击治疗小儿难治性肾病

目的 观察３１世纪小儿难治性肾病综合征（ＮＲＳ）患儿接受大剂量环磷酰胺（ＣＴＸ）间断静脉冲击的按期疗效。方法 ＣＴＸ每月一次,每次５００ｍｇ／ｍ＾２,静脉给药,连续≥６次,累积量≤１８５ｍｇ／ｋｇ。结果 ２７例完全缓解,总有效率８７．１％。出现胃肠道反应和肝功能异常各５例,分别占１６．１％。结论 大剂量ＣＴＸ 脉冲击治疗小儿ＲＮＳ近期疗效较好,副反应少。     

环磷酰胺冲击治疗儿童难治性肾病综合征30例疗效观察

儿童难治性肾病综合征３０例。本文用环磷酰胺冲击（ＣＴＸ一ＰＴ）治疗。结果：近期疗效９０％，２０／３０例在第一疗程有效，２６／３０例在第二疗程有效，２７／３０在第三疗程有效。近期副作用：轻度脱发３例，恶心、呕吐２例，１例外周血ＷＢＣ降为２．８Ｘ１０９／Ｌ结果表明：ＣＴＸ一ＰＴ不失为一种治疗ＲＮＳ有效、副作用少的方法。     

全胃切除术后肠外结合肠内营养支持

目的：对比全胃切除术后的肠外营养（ＰＮ）结合肠内营养（ＥＮ）与标准ＰＮ的支持效果。方法：１６例患者随机分为两组，两组患者从术后ｄ１到术后ｄ１１进行等氮、等热量营养支持。结果：（１）ＰＮ＋ＥＮ组体重下降（１．２±０．２２）ｋｇ，ＰＮ组体重下降（２．６１±０．１６）ｋｇ；（２）ＰＮ＋ＥＮ组１１ｄ累积氮平衡（２２３．３±３２．９）ｍｇ／ｋｇ，ＰＮ组１１ｄ累积氮平衡（１３４．６±１８．５）ｍｇ／ｋｇ；（３）ＰＮ＋ＥＮ组血清白蛋白下降（０．６±０．２）ｇ／Ｌ，ＰＮ组下降（６．０＋０．３）ｇ／Ｌ；（４）ＰＮ＋ＥＮ组德中谷氨酰胺下降（１８±６．４）μｍｏｌ／Ｌ，ＰＮ组下降（１０７±７．８）μｍｏｌ／Ｌ；（５）ＰＮ＋ＥＮ组血中Ｃ３补体下降（１１±３２）ｍｇ／Ｌ，ＰＮ组下降（１０２±６２）ｍｇ／Ｌ。结论：ＰＮ结合ＥＮ较标准ＰＮ更适合于全胃切除术后的肠功能恢复特点，有更好的代谢效果     

“QL”煎剂对全血细胞减少及骨髓抑制的实验研究

目的：观察“ＱＬ”剪剂对由环磷酰胺（ＣＴＸ）引起的大鼠全血细胞减少及骨髓抑制的疗效。方法：ＳＤ大鼠４０只,分为四组,对照组给生理盐水,三个实验组均皮下注射ＣＴＸ１．００ｍｇ／ｋｇ／只,实验Ⅱ,Ⅲ组用ＱＬ煎剂灌胃给药０．４ｍｇ／ｋｇ／只治疗,采血测试各项指标。     

环磷酰胺与甲基强的松龙冲击疗法治疗SLE合并肾脏受累的疗效比较

我们自１９９２年１０月以来,连续观察了２０例ＳＬＥ合并肾脏受累的患者,采用冲击疗法。其中１０例使用甲基强的松龙冲击,另１０例使用环磷酰胺冲击。现报告如下。１　病例和方法１１病例　均为已确诊的ＳＬＥ患者,２０例全为女性,年龄２０岁～４５岁,平均３４岁,病程平均３年,肾脏受累以急性肾炎综合征（血尿、蛋白尿、浮肿、尿少）起病。１２治疗方法　１０例ＳＬＥ患者用甲基强的松龙冲击,按１～２ｇ／ｄ的量静滴连用３天为一疗程,冲击前后都用强的松（１ｍｇ／ｋｇ）口服以维持症状。另１０例用环磷酰胺冲击,按１２ｍｇ…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.