The whitening effect of bleaching agents on tetracycline-stained rat teeth

This study compared the whitening effect of three bleaching agents on the teeth of rats and demonstrated differences in bleaching where dentin was exposed or enamel was thin. Thirty Albino rats were peritoneally injected with tetracycline solution daily for two weeks. Thirty-two disc-shaped specimens were cut from the crowns of incisors removed from sacrificed rats and were irradiated with UV light for 16 hours. Sections were stored in saline. Eight sections served as controls and were not bleached. Three bleaching agents (Opalescence, Rembrandt and Nite White) were applied to eight specimens each, five times a day for two weeks, and images of the sections were recorded at the following times: before bleaching (baseline), day 1, day 3, day 5, day 7, day 9, day 11 and day 14. Mean colors to demonstrate any change (deltaE) from baseline for each time period were as follows: control-9.78 (baseline), 9.17, 9.36, 9.65, 9.40, 9.99, 10.57, 11.36; Opalescence-10.08, (baseline) 7.63, 6.72, 6.04, 5.10, 4.87, 4.89, 4.27; Rembrandt-9.83 (baseline), 11.27, 9.55, 8.36, 7.75, 6.94, 7.11, 7.04; Nite White-10.44 (baseline), 9.92, 7.58, 6.80, 5.45, 5.05, 4.73, 4.01. All bleached teeth were lightened (p<.01). Another 56 tetracycline-stained rat incisors were UV irradiated for three days. Three different penetration depths were tested: penetration through lingual dentin and labial enamel (DN group), penetration through labial enamel only (RE group) and penetration through labial enamel covered with 1.0 mm human enamel (HE group). Specimens were bleached with Opalescence for one hour five times a day for one week or four weeks. A control group of unbleached teeth was also examined. Results (deltaE) were as follows: control--11.67; 1-week DN--13.55; 1-week RE--12.80; 1-week HE--12.07; 4-week DN--7.48; 4-week RE--7.50; 4-week HE--11.69. The color change in the 4-week DN and the 4-week RE groups showed the greatest reduction (p<.01).     

The effect of different bleaching agents on the surface texture of restorative materials

This blind in vitro study evaluated the effect of a home and an in-office bleaching agent on the surface texture of different tooth-colored restorative materials. Four composites (a hybrid, a flowable, a microhybrid and a nano-hybrid), an ormocer and a ceramic were used, and 2 bleaching agents were tested: 38% hydrogen peroxide and 15% carbamide peroxide. For 38% hydrogen peroxide, the surface morphology of the restorative materials was evaluated after the following time periods: before bleaching, after 15, 30 and 45 minutes of bleaching, 24 hours and 1 month after bleaching. For 15% carbamide peroxide, the time periods were: before bleaching, after 8 and 56 hours of bleaching and 24 hours and 1 month after bleaching. For the 4 composite materials and the ormocer, 2 samples groups were prepared; in 1 group, the specimens were polished and in the other, they stayed unpolished. For the ceramic group, polished samples were prepared. For every material, 3 samples per category and time period were prepared, respectively. Subsequently, the appropriate bleaching procedure was performed on samples of every group. Scanning electron micrographs were produced at 60x, 200x and 2000x magnifications of respective areas of the samples. The results showed that the effect of bleaching on the surface texture was material- and time-dependent. Within the limitations of this study, it was concluded that bleaching with 38% hydrogen peroxide and 15% carbamide peroxide did not cause major surface texture changes on the polished surfaces of the restorative materials.     

The effect of bleaching agents on the microhardness of dental aesthetic restorative materials

This study investigated the effects of three home bleaching agents on the microhardness of various dental aesthetic restorative materials. The restorative materials were: feldspatic porcelain, microfilled composite resin and light-cured modified glass-ionomer cement and the bleaching agents Nite-White (16% carbamide peroxide), Opalescence (10% carbamide peroxide and carbapol jel) and Rembrandt (10% carbamide peroxide jel). A total of 90 restorative material samples were prepared 1 cm diameter and 6 mm thick and kept in distilled water for 24 h before commencing bleaching which was carried out for 8 h day-1 for 4 weeks. Microhardness measurements were then made using a Tukon tester. Statistically significant differences with respect to unbleached controls were found only for the feldspatic porcelain and microfilled composite resins (P <0.05) for Nite-White and Opalescence. All the bleaching agents decreased the microhardness of the porcelain and increased that of the light cured modified glass-ionomer cement. For the composite resin, whereas Nite-White increased its microhardness, the other bleaching agents decreased it. There were no significant differences between the bleaching agents for any of the restorative materials.     

The effect of carbamide peroxide bleaching agents on the microhardness of dental ceramics

This study examined the effect of 10% and 16% carbamide peroxide bleaching agents on the surface microhardness of micro-particulate feldspathic ceramics (VM7 and VM13, Vita Zahnfabrik). Forty specimens (8-mm diameter, 2-mm thickness) were divided into four groups (n=10): GI-VM7 + 10% Whiteness, G2-VM7 + 16% Whiteness, G3-VM13 + 10% and G4-VM13 + 16% Whiteness. The home-use bleaching agents were applied for 8 hours on 15 days, and the specimens were stored in distilled water at 37 degrees C. The Vickers hardness number (HV) was determined for each specimen. Data were analyzed by the Wilcoxon and Mann-Whitney tests (p < 0.05). The microhardness values before exposure were: g1-433 (57); g2-486 (22); g3-509 (28); g4-518 (24), and after exposure: G1-349 (32); G2-496 (95); G3-519 (38); G4-502 (81). G2 exhibited a higher and significant difference than GI in VM7 groups, and the effect of bleaching concentration was shown to be significant by the Mann-Whitney test. And for VM13, both the Wilcoxon and Mann-Whitney tests showed no significant differences. When using 10% carbamide peroxide, the microhardness of VM7 ceramic was affected, and there were no effect on the microhardness between VM7 and VM13 ceramics when 16% carbamide peroxide was used.     

过氧化脲漂白剂对釉质显微硬度影响的实验研究

目的:评价不同载体和浓度的过氧化脲(carbam ide peroxide,CP)漂白剂对釉质表面显微硬度的影响。方法:在18个离体前磨牙的颊、舌面各制备2 mm×2 mm的平面,随机分为6组。分别用卡波姆(Car-bopol)、聚乙烯吡咯烷酮(PVP)或泊洛沙姆(poloxam er)为增稠载体的含11%、21%CP的漂白剂,在37℃100%湿度条件下每天漂白6~8 h,持续2周。漂白前、后测量釉质表面的显微硬度。21%CP治疗组在漂白治疗结束后继续浸入人工唾液2周,再次测量显微硬度。结果:11%CP漂白2周后,釉质的显微硬度与漂白前相比没有显著差异(P>0.05);21%CP漂白2周后,釉质的显微硬度明显低于漂白前(P<0.01),继续浸入人工唾液2周,显微硬度明显高于漂白后(P<0.05),但与漂白前相比仍有显著差异(P<0.05)。在相同CP浓度下,不同增稠载体组间的显微硬度没有显著差异(P>0.05)。结论:11%CP漂白剂对釉质的显微硬度没有影响,21%CP漂白剂可明显降低釉质的显微硬度,人工唾液可在一定程度上增加釉质的再矿化能力,不同的增稠载体对釉质的显微硬度没有明显影响。     

Cytotoxicity of intracanal bleaching agents on periodontal ligament cells in vitro

Intracoronal bleaching of nonvital teeth is a simple and conservative procedure for esthetic restoration of discolored teeth. However it is possible that damage to the periodontal ligament may occur if the bleaching agents contact this tissue. The purpose of this study was to examine the cytotoxicity of intracanal bleaching agents on human periodontal ligament (PDL) cells in vitro. Three bleaching agents, 30% hydrogen peroxide (H2O2), 2.0 g/ml sodium perborate (SP) solution, and 2.0 g/ml SP in H2O2, were diluted from 10(-3) to 10(-7) with Eagle's minimal essential medium and incubated with PDL cells isolated and cultured from extracted teeth. Cytotoxicity was assessed quantitatively by determining the amount of lactic dehydrogenase activity released from the cells after exposure to the agents for 24 or 72 h. Dose-response curves were plotted, and TD50 values (dilution causing the release of 50% of control lactate dehydrogenase activity) and 95% confidence limits determined. The rank order of the TD50 values after exposure for 24 h was SP in H2O2 (most toxic) > H2O2 > SP solution (least toxic). After 72 h SP in H2O2 still produced the greatest cytotoxic effect. However the SP solution was more cytotoxic than H2O2 at this time point. It is concluded that the mixture of SP with H2O2 was the most toxic to the PDL cells in vitro.     

The effect of bleaching agents on mercury release from spherical dental amalgam

To investigate the effect of carbamide peroxide, hydrogen peroxide, and over-the-counter home bleaching products on the release of mercury from spherical dental amalgam, 150 uniform amalgam specimens were prepared in clear acrylic blocks, aged for one week at 37 degrees C, and placed into individual polystyrene jars containing 20 mL of sterile saline. The specimens were divided into three groups of 50 and bleached in eight-hour cycles. All groups exposed to bleach showed increased mercury release over time.     

In vitro evaluation of the effect of a current bleaching agent on the electrochemical corrosion of dental alloys

The nightguard bleaching of discoloured teeth is currently performed by using % 10 carbamide peroxide. While the material is effective on the bleaching of natural teeth, it may deteriorate crowns, bridges and amalgam restorations placed in the mouth. The purpose of the present study was to investigate the effect of 10% carbamide peroxide on the electrochemical corrosion of various dental casting alloys used for fixed partial dentures and dental amalgam. Tafel extrapolation curves were obtained by the corrosion cell that facilitated simulation of the oral environment and comparison of the corrosion rates of alloys and amalgam was accomplished. The results revealed that unpolished amalgam and nickel-chromium alloy samples had the most and noble alloys had the least corrosion rate.     

In vitro penetration of bleaching agents into the pulp chamber

AIM: To investigate pulp chamber penetration of bleaching agents in teeth following restorative procedures. METHODOLOGY: Bovine lateral incisors were sectioned 3 mm apical to the cemento-enamel junction and the coronal pulpal tissue was removed. Teeth were divided into six groups (n = 10): G1, G2 and G3 were not submitted to any restorative procedure, while G4, G5 and G6 were submitted to Class V preparations and restored with composite resin. Acetate buffer was placed in the pulp chamber and treatment agents were applied for 60 min at 37 degrees C as follows: G1 and G4, immersion into distilled water; G2 and G5, 10% carbamide peroxide (CP) exposure; G3 and G6, 35% CP bleaching. The buffer solution was removed and transferred to a glass tube where leuco crystal violet and horseradish peroxidase were added, producing a blue solution. The optical density of the blue solution was determined spectrophotometrically at 596 nm. A standard curve made with known amounts of hydrogen peroxide was used to convert the optical density values of the coloured samples into microgram equivalents of hydrogen peroxide. Data were submitted to anova and Tukey's test (5%). RESULTS: Amounts of hydrogen peroxide found in the pulp chamber of G2 and G5 specimens (0.1833 +/- 0.2003 micro g) were significantly lower (P = 0.001) when compared to G3 and G6 specimens (0.4604 +/- 0.3981 micro g). Restored teeth held significantly higher (P = 0.001) hydrogen peroxide concentrations in the pulp chamber than intact teeth. CONCLUSION: Higher concentrations of the bleaching agent produced higher levels of hydrogen peroxide in the pulp chamber, especially in restored teeth.     

The effect of bleaching agents on the surface hardness of tooth colored restorative materials

AIM OF THE STUDY: The aim of this study was to evaluate the effect of at-home (Opalesence/Dr. kit 15%, Ultradent, Products, Inc. South Jordan, UT, USA) and in-office (Superoxol 35%, Sultan Chemists, Inc., Englewood, NJ, USA) bleaching on the surface hardness of the following tooth colored restorative materials: composite resin, Point-4 (P4), Kerr Corporation, Orange, CA, USA; ormocer, Admira (AD),VOCO, Germany; compomer Dyract AP (DY), Dentsply DeTrey GmbH, Germany; and resin modified glass ionomer cement, Fuji II LC (FL), GC Corporation, Japan. METHODOLOGY: Sixty specimens were prepared; 15 specimens of each material (each group n = 5, control after 15 days, at-home and in-office). All specimens were stored in distilled water at room temperature for 24 hrs before testing. A universal testing machine (Micromet 2100 series micro hardness testers) was used for testing Vicker's surface hardness for the three groups for every tested material. All results were statistically analyzed with one way analysis of variance (ANOVA), Post hoc Tukey HSD tests (P < 0.05), and percentage changes for Tukey. RESULTS: All the tested materials showed an increase in Vicker's surface hardness between base line (24 hrs) and the control group after 15 days storage in distilled water except DY which showed a decrease in surface hardness. All tested materials showed a decrease in surface hardness from control group after 15 days and both at-home and in-office bleaching agents except DY which showed increased values. SIGNIFICANCE: At-home as well as in-office bleaching agents have a softening effect on some tooth colored restorative material, and the patient must be aware before using them.     

In vitro tooth color change with repeated bleaching.

The interest of patients in vital tooth bleaching has created a demand for treatment. However, few objective data are available to help determine the number of bleaching appointments necessary and the lightening that can be expected. The purpose of this study was to measure color changes in bleached extracted teeth and compare these to control groups. Group A was etched and bleached with 35% stabilized hydrogen peroxide. Group B was treated the same as group A, but the bleaching solution was replaced with distilled water. Group C was not treated, but was stored in water. Mean color difference after one treatment was 3.33 for group A, 1.67 for group B, and 0.48 for group C. After six treatments, the overall color difference was 3.82, 2.41, and 1.38 for groups A, B, and C, respectively (P less than .01). Color changes beyond those found after the first treatment were small, suggesting that there was little benefit in repeated bleachings under the conditions of this study.     

An in vitro comparison of different bleaching agents in the discolored tooth

Extracted teeth with intact crowns were stained to compare and evaluate the effectiveness of four bleaching agent combinations. Forty-two permanent and 12 deciduous teeth were used. The bleaching agents were placed into coronal pulp chambers and then evaluated and rated at 1-, 3-, 7-, and 14-day and 6-month intervals. New sodium perborate combined with fresh Superoxol was the most effective bleaching agent with 93% success (the final shade was as light as the prestained shade). New sodium perborate and 1-yr-old Superoxol was 73% successful. New sodium perborate with distilled water and old sodium perborate with distilled water were both 53% successful. Deciduous teeth demonstrated a response to bleaching agents similar to that of permanent teeth. Color regression after 6 months was found in 4% of the cases. When it occurred, the degree of color regression was minimal.     

The effect of propolis on the bond strength of composite resin to enamel after intracoronal bleaching with different bleaching agents

This study evaluated the effect of propolis as an antioxidant agent on bond strength to enamel after intracoronal bleaching. A total of 160 incisors were endodontically treated. Sixteen teeth were served as control, and the remaining teeth were randomly divided into three main groups according to the bleaching agent used; group 1: Sodium perborate (SP); group 2: Carbamide peroxide (CP); group 3: Hydrogen peroxide (HP). After bleaching, the samples were divided into three subgroups; subgroup A: no antioxidant agent application, subgroup B: sodium ascorbate (SA), subgroup C: propolis (PP). After the antioxidant agents application, the sample's surfaces were washed and dried. After adhesive application, composite resin cylinders were applied to enamel surfaces using tygon tubes and a shear bond strength test was performed. The use of PP significantly decreased the bond strength of composite resin to the enamel (p < 0.05). Using propolis as an antioxidant agent adversely affects the bond strength to enamel after intracoronal bleaching.     

过氧化脲漂白剂的体外细胞毒性研究

目的:评价不同增稠载体过氧化脲漂白剂的细胞毒性,并与同类商品漂白剂进行比较.方法:采用琼脂覆盖法通过直径5咖的滤膜圆片,分别评价以Carbopol或PVP为增稠载体的100 g/L、200 g/L过氧化脲漂白剂及同种浓度Opalescence漂白凝胶对L929小鼠成纤维细胞的毒性作用.结果:以Carbopol或PVP为增稠载体的100g/L、200 g/L CP漂白剂和Opalescence 100 g/L、200 g/LCP漂白凝胶的细胞毒性均为2级,中度毒性.结论:不同增稠载体的过氧化脲漂白剂和Opalescence漂白凝胶的细胞毒性没有显著差别.     

The effect of home bleaching agents on the surface roughness of tooth-colored restoratives with time

This study evaluated the effects of home bleaching agents on the surface roughness of composite restoratives. Two home bleaching gels (10% and 15% carbamide peroxide, Opalescence) and five different tooth-colored restorative materials from the same manufacturer (3M-ESPE) were selected. They included microfill (Filtek A110 [FO]), flowable (Filtek Flow [FF]), polyacid-acid modified (F2000 [FT]) and minifill (Z100 [ZO]; Filtek Z250 [ZT]) composites. Thirty-six specimens of each material were fabricated, randomly divided into three groups (n=12) and treated as follows: Group 1-Stored in distilled water, Group 2-Bleached with 10% carbamide peroxide (CP) eight hours/day; Group 3-Bleached with 15% CP eight hours/day. All treatment was conducted at 37 degrees C and fresh gel applied and rinsed off daily for eight weeks. For the bleached groups, the specimens were stored in distilled water at 37 degrees C during the hiatus periods. All the specimens were subjected to roughness testing (Ra) at weeks 0, 1, 2, 4, 6 and 8 using a profilometer. The results were analyzed using general linear model with Scheffe's post-hoc tests at significance level 0.05. The results showed that the effect of bleaching on surface roughness was material and time dependent. ZT was not affected by bleaching treatment, while FT was significantly roughened after one week of bleaching with 15% CP compared to the control group. FO, FF and ZO were not significantly roughened until eight weeks of bleaching. Repolishing or replacement of tooth-colored restorations may be required after bleaching procedures.