Multiple genetic alterations and behavior of cellular biology in gastric cancer and other gastric mucosal lesions:H.pylori infection, histological types and staging

AIM:To investigate the expression of multiple genes and the behavior of cellular biology in gastric cancer (GC) and other gastric mucosal lesions and their relations to Helicobacter pylori (H. pylori) infection, tumor staging and histological subtypes.METHODS:Three hundred and twenty seven specimens of gastric mucosa obtained via endoscopy or surgical resection, and ABC immunohistochemical staining were used to detect the expression of p53, p16, Bcl-2 and COX-2 proteins.H. pylori was determined by rapid urea test combined with patholo-gical staining or 14 Curea breath test. Cellular image analysis was performed in 66 patients with intestinal metaplasia (IM) and/or dysplasia (Dys). In 30 of them, both cancer and the paracancerous tissues were obtained at the time of surgery. Histolo-gical pattern, tumor staging, lymph node metastasis, grading of differentiation and other clinical data were studied in the medical records.RESULTS:p16 expression of IM or Dys was significantly lower in positive H. pylori chronic atrophic gastritis (CAG) than those with negative H. pylori (CAG: 54.8% vs 88.0%, IM:34.4% vs 69.6%, Dys: 23.8% vs 53.6%, all P < 0.05), Bcl-2 or COX-2 expression of IM or Dys in positive H. pylori cases was signi-ficantly higher than that without H. pylori (Bcl-2: 68.8% vs 23.9%, 90.5% vs 60.7%; COX-2: 50.0% vs 10.8%, 61.8% vs 17.8%; all P <0.05). The mean number of most parame-ters of cellular image analysis in positive H. pylori group was significantly higher than that in negative H. pylori group (Ellipser: 53 plus minus 14, 40 plus minus 12&mgr;m, Area(1): 748 plus minus 572, 302 plus minus 202&mgr;m(2), Area(2): 3050 plus minus 1661, 1681 plus minus 1990&mgr;m(2), all P< 0.05; Ellipseb: 79 plus minus 23, 58 plus minus 15&mgr;m, Ratio-1: 22% plus minus5%,13% plus minus4%,Ratio-2:79% plus minus17%,53% plus minus20%,all P<0.01). There was significant correl-ation between Bcl-2 and histologic pattern of gastric carcinoma, and between COX-2 and tumor staging or lymph node metasta sis (Bcl-2: 75.0% vs16.7%; COX-2: 76.0% vs 20.0%, 79.2% vs 16.7%; all P< 0.05).CONCLUSION:p16, Bcl-2, and COX-2 but not p53 gene may play a role in the early genesis/progression of gastric carcinoma and are associated with H. pylori infection. p53 gene is relatively late event in gastric tumorigenesis and mainly relates to its progression. There is more cellular-biological behavior of malignant tumor in gastric mucosal lesions with H. pylori infec-tion. Aberrant Bcl-2 protein expression appears to be preferentially associated with the intestinal type cancer. COX-2 seems to be related to tumor staging and lymph node metastasis.     

Correlation between expression of cyclooxygenase-2 and angiogenesis in human gastric adenocarcinoma

AIM: To evaluate the expression of cyclooxygenase (COX2) and the relationship with tumor angiogenesis and advancement in gastric adenocarcinoma.METHODS: Immunohistochemical stain was used for detecting the expression of COX-2 in 45 resected specimens of gastric adenocarcinoma; the monoclonal antibody against CD34 was used for displaying vascular endothelial cells, and microvascular density (MVD) was detected by counting of CD34-positive vascular endothelial cells. Paracancerous tissues were examined as control.RESULTS: Immunohistological staining with COX-2-specific polyclonal antibody showed cytoplasmic staining in the cancer cells, some atypical hyperplasia and intestinal metaplasia,as well as angiogenic vasculature present within the tumors and prexisting vasculature adjacent to cancer lesions. The rate of expression of COX-2 and MVD index in gastric cancers were significantly increased, compared with those in the paracancerous tissues (77.78 vs 33.33 %, 58.13±19.99 vs 24.02±10.28, P＜0.01, P＜0.05, respectively). In 36 gastric carcinoma specimens with lymph node metastasis, the rate of COX-2 expression and MVD were higher than those in the specimens without metostasis (86.11 vs 44.44 %,58.60±18.24 vs 43.54±15.05, P＜0.05, P＜0.05, respectively).The rate of COX-2 expression and MVD in the specimens with invasive serosa were significantly higher than those in the specimens without invasion to serosa (87.88 vs 50.0 %,57.01±18.79 vs42.35±14.65, P＜0.05, P＜0.05). Moreover,MVD in COX-2-positive specimens was higher than that in COX-2-negative specimens (61.29±14.31 vs 45.38±12.42,P＜0.05). COX-2 expression was positively correlated with MVD (r=0.63, P＜0.05).CONCLUSION: COX-2 expression might correlate with the occurance and advancement of gastric carcinoma and is involved in tumor angiogenesis in gastric carcinoma. It is likely that COX-2 by inducing angiogenesis can be one of mechanisms which promotes invasion and metastasis of gastric carcinoma. It may become a new therapeutic target for anti-angiogenesis.     

ATDC在胃癌中的表达及其与幽门螺杆菌感染的关系

背景：ATDC又名TRIM29,属于TRIM蛋白家族,具有促进细胞增殖和抑制电离辐射敏感性的功能。研究显示其在某些恶性肿瘤中呈高表达,可作为胃癌淋巴结转移的标记,并参与了胰腺癌的生长和转移。目的：明确ATDC在胃癌中的作用,并初步探讨ATDC与幽门螺杆菌（H.pylori）相关胃癌的关系。方法：应用免疫组化方法检测72例胃癌患者癌组织和相应癌旁组织中的ATDC表达,分析其在胃癌组织中的表达与胃癌临床病理特征和患者H.pylori感染状态的关系。结果：胃癌组织ATDC阳性表达率显著高于相应癌旁组织（76.4%对2.8%,P〈0.05）。中低分化、伴淋巴结转移或远处转移、伴H.pylori感染的胃癌患者,胃癌组织ATDC阳性表达率和表达强度分别显著高于高分化、不伴转移、不伴H.pylori感染的胃癌患者（P〈0.05）,ATDC表达与胃癌患者的性别无关。结论：胃癌组织中的ATDC表达与胃癌组织学分级、转移和患者的H.pylori感染状态相关,提示其可能参与了胃癌的发生、发展,并可能与H.pylori相关胃癌有一定联系。     

Relationship between expression and distribution of cyclooxygenase-2 and bcl-2 in human gastric adenocarcinoma

AIM: To explore expression and distribution features of COX-2 and bcl-2 in human gastric adenocarcinoma tissues and to study its biological significance. METHODS: Totally 36 human gastric carcinoma samples were enrolled in this study (cardiac adenocarcinoma 16 cases, distal gastric adenocarcinoma 20 cases). The expressions of COX-2 and bcl-2 in cancerous tissues and corresponding para-cancerous tissues were investigated by immunohistochemistry using COX-2 polyclonal antibody and bcl-2 monoclonal antibody. The normal gastric mucosa tissues were used as control. RESULTS: The expressions of COX-2 and bcl-2 in gastric carcinoma were significantly higher than that in the para-cancerous tissues (77.8% vs 47.2%, P<0.01, 80.56% vs 58.33%, P<0.05). The expression of COX-2 in cardiac adenocarcinoma was remarkably higher than that in the distal gastric carcinoma (93.8% vs 65.0%, P<0.01). The expression of COX-2 was mainly localized in the cytoplasm of tumor cells and partly in the nucleus. There is a transition of the COX-2 cytoplasmic positivity to nucleic in tumor cells with the increase of gastric carcinoma pathological grade. Interstitial macrophages, fibroblasts and vascular endothelial cells also expressed COX-2. The tissues with higher expression of COX-2 also expressed high level of bcl-2 protein. CONCLUSION: Abnormal expression pattern of COX-2 within the tissues of human gastric cancer is correlated with tumor location and lymph node metastasis. COX-2 may regulate expression of apoptosis suppressor gene (bcl-2) through interaction of tumor cells and stromal cells and play an important role in the generation and development of tumors, which will be of great help in developing new methods for antitumor therapy.     

Detection and location of Helicobacter pylori in human gastric carcinomas

AIM: To define the infection status of Helicobacter pylori in 109 patients with gastric cancers and H pylori localization in gastric carcinoma tissues in South China. METHODS: The incidence of H pylori infection in gastric carcinomas was estimated by polymerase chain reaction (PCR), simultaneously; both morphological features and the localization of H pylori in gastric carcinomas were demonstrated by Warthin-Starry (WS) staining. The relationships between H pylori infection and the clinical-pathologic factors of gastric carcinomas were analyzed by software SPSS10.0. RESULTS: H pylori was found in 42 (39.03%) and 58 (53.21%) cases of 109 patients with gastric carcinomas by PCR and WS, respectively. H pylori infection rate detected in gastric carcinomas by WS was higher than that by PCR (X2=9.735, P<0.005<0.01). WS stain showed that H pylori existed in the gastric antrum mucus, mucosal gland of normal tissues adjacent to gastric carcinomas and the gland, mucus pool of cancer tissues. The positive rate of H pylori in normal tissues adjacent to carcinomas was higher than that in cancer tissues (X2=15.750, P<0.005 <0.01). No significant differences in age, sex, site, histological types and lymph node metastasis were found between H pylori-positive gastric carcinomas and H pylori-negative cases by both methods, but there were statistically significant differences of H pylori positive rate between early and advanced stage of gastric carcinomas (X2= 4.548 or 5.922, P= 0.033 or 0.015<0.05). CONCLUSION: These results suggested that H pylori infection might play a certain role in the early stage of carcinogenesis of human gastric mucosa epithelia.     

环氧合酶-2、基质金属蛋白酶-9和核增殖抗原在胃癌组织中的表达及临床意义

目的 探讨环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)及核增殖抗原(Ki67)在胃癌中的表达与胃癌发生、浸润和转移的关系.方法 选择2003年1月至2005年12月手术切除、病理证实的胃癌存档蜡块标本58例,其中男37例,女21例.年龄31～76岁,中位年龄58.2岁.另取上述胃癌根治术患者距肿瘤5～6 cm的癌旁组织作对照.采用免疫组化法检测胃癌组织中COX2、MMP-9、Ki67的表达.结果 COX-2、MMP-9在胃癌组织中的表达率分别为82.76%和68.9%,均高于对照组(37.93%和24.14%,P＜0.01).COX-2、MMP-9的表达与胃癌患者性别、年龄、部位及大小无相关性(P＞0.05),与胃癌浸润深度、淋巴结转移及TNM分期有关(P＜0.05),MMP-9还与胃癌分化程度相关(P＜0.05).COX-2与MMP-9在胃癌组织中的表达有相关性(P＜0.05,C=0.359).MMP-9、COX-2表达阳性者Ki67表达水平高于阴性者,两者间差异有统计学意义(P＜0.01).结论 COX-2、MMP-9、Ki67在胃癌浸润、转移过程中起重要作用,共同促进肿瘤的发生、发展.     

肿瘤相关基因NAG6、NAG-7、BRD7在胃癌中的表达

目的　NAG6、NAG 7、BRD7为最近克隆的肿瘤相关新基因 ,对它们在胃癌及癌旁组织中的表达进行检测 ,探讨这些基因在胃癌发生发展中的作用。方法　采用RT PCR、Northern杂交、点杂交方法检测 34例胃癌和癌旁组织中这些基因的表达。结果　胃癌组织中 ,有 61 .8% (2 1 / 34)NAG6基因表达缺失 ,NAG6在胃癌组织中的表达较癌旁组织显著下调 (P <0 .0 1 ) ,但NAG6表达下调与胃癌淋巴结或远处转移无明显关系 (P >0 .0 5)。NAG 7基因在胃癌和癌旁组织中表达率分别为 88.2 % (30 / 34)和 82 .3 % (2 8/ 34)。BRD7基因在胃癌及癌旁组织中均有表达。RT PCR、Northern杂交、点杂交结果均显示NAG 7、BRD7基因在胃癌与癌旁组织之间的表达差异无显著性。同时 ,点杂交也证实NAG6在胃癌组织中表达下调。结论　NAG6基因在胃癌组织中表达显著下调 ,这可能在胃癌的发生发展过程中起重要作用 ,但可能与淋巴结或远处转移无关。BRD7、NAG 7基因在胃癌中未发现表达水平改变 ,初步提示这两种基因可能在胃癌的发生发展中不起作用     

幽门螺杆菌感染患者胃癌及癌旁组织中p53,p16和bc1—2基因的表达

研究幽门螺杆菌（Ｈ．ｐｙｌｏｒｉ）感染胃癌得胃粘膜病变中抑癌基因ｐ５３、ｐ１６和关键性凋亡调节基因ｂｃ１－２蛋白的表达,进一步探讨Ｈ．ｐｙｌｏｒｉ在胃癌发生、发展过程中作用的分子机制。方法：胃镜检查及外科手术中取４０例胃癌患者的癌组织和癌旁２ ｃｍ处组织各２块,石蜡包埋,切片ＨＥ染色作病理诊断及免疫组化检查ｐ５３、ｐ１６及ｂｃ１－２蛋白的表达。Ｈ．ｐｙｌｏｒｉ阳性由快速尿素酶试验结合病理染色／＾１     

胰腺癌组织中COX-2、VEGF-C表达和淋巴管密度

目的 检测胰腺癌及癌旁组织中COX-2、VEGF-C的表达与淋巴管密度(LVD),探讨它们之间的关系.方法 应用组织芯片技术和免疫组化方法检测40例胰腺癌及相应癌旁组织和12例远处正常胰腺组织中COX-2、VEGF-C的表达及LVD,分析它们之间的关系,以及与临床病理指标的关系.结果 胰腺癌组织COX-2、VEGF-C表达阳性率分别为70.0%(28/40)和67.5%(27/40),显著高于癌旁组织的42.5%(17/40)和35.O%(14/40,P＜0.01),也显著高于正常胰腺的8.3%(1/12)和25.0%(3/12).癌、癌旁及正常胰腺的LVD分别为4.75±2.77、15.20±4.70和1.67±1.15,以癌旁组织最高(P＜0.01).癌组织COX-2表达与肿瘤分化程度、淋巴结转移相关;VEGF-C的表达与淋巴结转移相关.癌旁组织LVD与肿瘤分化程度、淋巴结转移有关,并与癌组织COX-2、VEGF-C表达存在一定相关性.结论 胰腺癌新生淋巴管主要存在于癌旁,淋巴管的形成可能有COX-2及VEGF-C的参与.     

microRNA-101和环氧合酶-2在胃癌中的表达及其临床意义

背景：microRNAs是一类对靶基因表达具有转录后调控作用的非编码小RNA。microRNA-101（miR-101）在多种恶性肿瘤中呈低表达,而过表达外源性miR-101可发挥肿瘤抑制作用。前期体内、外实验发现外源性miR-101可抑制胃癌细胞增殖和环氧合酶-2（COX-2）表达。目的：检测胃癌组织中的miR-101、COX-2表达并探讨其临床意义。方法：收集手术切除胃癌组织和配对癌旁非癌组织标本30例,以实时荧光定量RT—PCR检测miR-101、COX-2mRNA表达,分析两者间以及两者与胃癌主要临床病理特征的关系。结果：绝大多数胃癌组织中miR-101表达低下,COX-2mRNA则呈过表达。胃癌组织与癌旁非癌组织间miR-101、COX-2mRNA表达量差异显著（P〈0．01）,且两者表达在癌组织和癌旁组织中均呈负相关（癌组织：r=-0．767,P=0．000;癌旁组织：r=-0．718,P=0．000）。TNMⅢ、IV期胃癌和伴淋巴结转移的胃癌中,miR-101表达分别显著低于TNMI、Ⅱ期病例和无淋巴结转移病例（P〈0．05）,COX-2mRNA表达分别显著高于TNMI、Ⅱ期病例和无淋巴结转移病例（P〈0．05）。结论：miR-101与COX-2之间的负相关性可能有助于胃癌的临床诊断;miR-101表达低下伴COX-2过表达与胃癌临床进展和转移有关,对预后判断有一定参考价值。     

SOCS2和STAT3蛋白的表达及其与胃癌生物学行为的关系

目的:探讨SOCS2和STAT3蛋白在胃癌组织中的表达及其与胃癌生物学行为的关系.方法:应用免疫组织化学方法检测55例胃癌组织和55例正常胃组织中SOCS2和STAT3蛋白的表达情况,并分析二者与胃癌分化程度、淋巴结转移、浸润深度和临床分期及患者性别、年龄之间的关系,同时分析二者的相关性.结果:SOCS2在胃癌组织中的阳性表达率为25.5%,明显低于正常胃组织中的91.1%(P<0.05),且表达与分化程度、淋巴结转移、临床分期相关(P<0.05);STAT3在胃癌组织中的阳性表达率为72.7%,明显高于正常组织的18.2%(P<0.05),且表达与分化程度、淋巴结转移、浸润深度和临床分期相关(P<0.05),SOCS2与STAT3的表达呈负相关(r=-0.486,P<0.01).结论:SOCS2在胃癌组织中低表达,STAT3异常活化可促进细胞的过度增殖,促进胃癌的发展,二者的相互调节在胃癌发生发展中起重要作用.     

血小板增多、环氧化酶-2在老年胃癌中表达及预后

目的　研究血小板增多、环氧化酶 2 (COX 2 )表达与老年胃癌预后的关系。方法　回顾分析了 92例老年胃癌患者血小板增多、87例老年胃癌COX 2表达情况 ,并分析其与胃癌临床病理特征关系及预后影响。结果　胃癌患者血小板增多发生率为 2 8.2 6 % ,COX 2蛋白表达阳性率为 52 .5 % ,并且二者表达呈正相关 (P <0 .0 1 )。血小板增多、COX 2表达与肿瘤大小、浸润深度、淋巴结转移、TNM分期、血管癌栓密切相关 (P <0 .0 1 ) ,血小板增多、COX 2阳性表达与胃癌预后呈负相关。多因素分析表明 ,血小板增多是继TNM分期、淋巴结转移之后影响胃癌患者生存的独立预后因素。结论　血小板增多与COX 2表达密切相关 ,二者与胃癌生长、浸润关系密切 ,可作为估计老年胃癌预后的重要因素。     

结肠癌中骨桥蛋白与环氧化酶-2的表达及临床意义

目的研究骨桥蛋白(OPN)与环氧化酶-2(COX-2)在结肠癌组织中的表达水平,并探讨两者与结肠癌转移浸润的关系。方法应用免疫组化法检测60例结肠癌组织和16例癌旁正常组织中OPN与COX-2的表达,并用SPSS 16.0统计软件分析其表达水平与临床病理特征的关系及二者的表达相关性。结果 OPN在结肠癌组织中表达的阳性率(70.0%)高于癌旁正常结肠组织(18.8%),差异具有统计学意义(P<0.01);COX-2在结肠癌组织中表达的阳性率(75.0%)高于癌旁正常结肠组织(37.5%),差异具有统计学意义(P<0.01)。OPN和COX-2蛋白的表达与患者的性别、年龄、肿瘤大小无关,与肿瘤分期、淋巴结转移有关(P<0.05),OPN与COX-2蛋白在结肠癌组织中表达呈正相关。结论 OPN和COX-2在结肠癌的发生发展中起重要作用,联合检测可作为判断结肠癌恶性程度和预后的指标。     

Survivin、COX-2和VEGF在胃癌中的表达及其与预后的意义

目的研究生存素(Survivin)、环氧合酶-2(COX-2)和血管内皮生长因子(VEGF)在胃癌组织中的表达,探讨它们表达的关系及其与胃癌预后的关系。方法选取淮南东方医院集团总医院1992~2002年10年间行根治性手术治疗,临床、病理和随访资料齐全的胃癌患者65例,应用免疫组化S-P技术,检测Survivin、COX-2和VEGF在胃癌组织中表达。结果早期胃癌的5年生存率为95.2%(20/21),中期胃癌的5年生存率为63.6%(7/11)。中期胃癌组Survivin阳性表达高于早期胃癌组(73.6%vs 66.7%,P<0.05),其VEGF阳性表达和微血管密度(MVD)平均值高于早期胃癌组(P<0.05)。Survivin和COX-2在慢性萎缩性胃炎中的表达明显低于不典型增生者(P<0.05),在癌组织中的表达明显高于非癌组织(P<0.05)。胃癌组中的VEGF阳性表达率和MVD平均值均明显高于非癌组,且与胃癌浸润深度有关(P<0.05)。胃癌中Survivin、VEGF阳性表达的MVD值显著高于Survivin、VEGF阴性表达者(P<0.05);Survivin、COX-2、VEGF及MVD值与胃癌淋巴结转移、血管浸润均密切相关(P<0.05);Survivin、COX-2阳性表达及VEGF阳性表达者5年生存率明显低于阴性或低表达者(P<0.05)。多因素分析显示,淋巴结转移、浸润深度、Survivin表达、VEGF表达均为胃癌独立的预后因素。结论Survivin、COX-2、VEGF与胃癌的生长和浸润转移关系密切,可以作为反映胃癌生物学行为和判断预后的有效指标。     

环氧化酶2在大肠癌中的表达及其与大肠癌生物学特性的关系

[目的]通过观察环氧化酶2（Cyclooxygenase-2,COX-2）在大肠腺瘤、大肠癌中的表达及其与大肠癌生物学特性的关系,初步探讨其在大肠癌发生、发展过程中的作用机制。[方法]应用免疫组织化学染色法对78例大肠癌组织、21例大肠腺瘤组织和13例正常大肠黏膜组织进行免疫组化染色;应用它检验分析COX-2的表达情况及其与大肠癌临床病理特征的关系。[结果]大肠癌组织中COX-2阳性表达率为78．21％,明显高于大肠腺瘤的52．38％和正常大肠黏膜组织的7．69％（P〈0．05）;COX-2表达与大肠癌的Duke＇s分期、分化程度、淋巴结转移有相关性（P〈0．05）,而与大肠癌患者的性别、组织学类型无关（P〉0．05）。[结论]COX-2在大肠癌组织中表达率明显高于大肠腺瘤,而正常大肠黏膜中表达率极低或不表达;COX-2表达与大肠癌生物学特性有明显相关性。     

Expression of vascular endothelial growth factor C and chemokine receptor CCR7 in gastric carcinoma and their values in predicting lymph node metastasis

AIM: To study the expression of vascular endothelial growth factor C (VEGF-C) and chemokine receptor CCR7 in gastric carcinoma and to investigate their associations with lymph node metastasis of gastric carcinoma and their values in predicting lymph node metastasis. METHODS: The expression of VEGF-C and CCR7 in gastric carcinoma tissues obtained from 118 patients who underwent curative gastrectomy was examined by immunohistochemistry. Among these patients, 39 patients underwent multi-slice spiral CT (MSCT) examination. RESULTS: VEGF-C and CCR7 were positively expressed in 52.5 and 53.4% of patients. VEGF-C expression was more frequently found in tumors with lymph node metastasis than those without it (P<0.001). VEGF-C expression was also closely related to lymphatic invasion (P<0.001), vascular invasion (P<0.01), and TNM stage (P<0.001). However, there was no significant correlation between VEGF-C expression and age at surgery, gender, tumor size, tumor location, Lauren classification, and depth of invasion. CCR7 expression was significantly higher in patients with lymph node metastasis compared with those without lymph node metastasis (P<0.001) and was also associated with tumor size (P<0.01), depth of invasion (P<0.001), lymphatic invasion (P<0.001), and TNM stage (P<0.001). However, the presence of CCR7 had no correlation to age at surgery, gender, tumor location, Lauren classification, and vascular invasion. Among the 39 patients who underwent MSCT examination, only CCR7 expression was related to lymph node metastasis determined by MSCT (P<0.05). In the current retrospective study, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of VEGF-C and CCR7 expression in the diagnosis of lymph node metastasis for patients with gastric carcinoma were 73.8%, 70.2%, 72.6%, 71.4% and 72.0%, and 82.0%, 77.2%, 79.4%, 80.0% and 79.7%, respectively. After subdivision according to the combination of VEGF-C and CCR7 expression, receiver operating characteristic (ROC) analysis showed that the accuracy of the combined examination of VEGF-C and CCR7 expression in predicting lymph node metastasis was relatively high (area under ROC curve [Az]=0.83). CONCLUSION: The expression of VEGF-C and CCR7 is related to lymph node metastasis of gastric carcinoma and both of them may become new targets for the treatment of gastric carcinoma. Furthermore, the combined examination of VEGF-C and CCR7 expression in endoscopic biopsy specimens may be useful in predicting lymph node metastasis of gastric carcinoma and deciding the extent of surgical lymph node resection.     

胃癌及癌前病变组织中CD44v6表达的意义

目的探讨CD44v6基因表达与胃癌发生及胃癌生物学行为的关系.方法应用抗CD44v6蛋白的单克隆抗体,采用免疫组化ABC方法对正常胃粘膜(n=10)、各级胃粘膜异型增生(轻度n=16,中度n=12,重度n=14)、早期胃癌(n=16)及进展期胃癌(n=52)进行研究,并与胃癌类型、大小、有无淋巴结转移等作了比较分析.结果正常胃粘膜CD44v6为阴性,随着胃粘膜病变的进展,CD44v6蛋白的表达率逐渐升高,至进展期胃癌,表达率达到顶峰.轻、中、重度异型增生表达率分别为12%,33%,43%;早期胃癌及进展期胃癌的表达率分别为44%和73%.各级异型增生表达率之间的差异无显著性,而进展期胃癌表达率显著高于早期胃癌(P＜0.05),淋巴结转移组的表达率显著高于淋巴结未转移组(82%vs56%,P＜0.05),肠型胃癌的表达率高于弥漫型胃癌(78%vs56%,P＜0.05),CD44v6蛋白的表达与胃癌肿块大小无相关性.结论胃粘膜重度异型增生在CD44v6基因表达上已具有明显的潜在恶性趋势,CD44v6基因表达阳性的胃癌具有更强的浸润及淋巴结转移的能力.     

COX-2基因表达与胃癌淋巴结转移的关系

目的 研究COX-2 mRNA及蛋白在胃癌组织中的表达,探讨COX-2与胃癌淋巴结转移的关系。方法 采用 RT-PCR方法检测30例新鲜胃癌标本及相匹配的癌旁正常组织COX-2 mRNA的表达;用免疫组化方法检测45例胃癌石蜡标本COX-2蛋白表达。结果 胃癌组织中存在COX-2 mRNA(73.3％)及蛋白(62.2％)表达。伴淋巴结转移者,COX-2 mRNA及蛋白表达高于无转移者,(P<0.05)。结论 COX-2基因在胃癌中表达增高,且与胃癌淋巴结转移明显相关。     

Wnt信号通路靶基因GS mRNA及蛋白在胃癌中的表达

目的: 探讨Wnt信号通路的靶基因GS mRNA及蛋白在胃癌中的表达及其临床意义.方法: 荧光实时定量PCR(FQ-PCR)检测52例胃癌组织及癌旁正常组织GS mRNA表达;免疫组织化学技术(SP法)检测97例胃癌组织、30例癌旁正常组织及10例肠化生组织中GS蛋白表达水平差异.结果: 癌组织和癌旁正常组织GS mRNA表达有显著差异(25.508±5.090 vs 13.001±2.040,P<0.05). 癌组织GS蛋白表达与组织学类型、Lauren分型及淋巴结转移密切相关(χ2= 26.994,54.929,5.173,均P<0.05),与肿瘤大小、TNM分期、远处转移及患者的性别和年龄等无明显相关.结论: GS mRNA和蛋白高表达同胃癌生物学行为密切相关,可能与胃癌的发生、发展及预后有关.