宫内发育迟缓大鼠软骨生长板生长轴变化的研究

目的研究宫内发育迟缓（IUGR）大鼠长骨生长、骨骺软骨生长板形态学和IGF-1表达的变化,探讨其生长轴受损的机制。方法采用母鼠妊娠期饥饿法建立IUGR动物模型。根据新生鼠及其生后第4周时体重和身长分为有生长追赶IUGR组、无生长追赶IUGR组和正常对照组;测量4周龄时各组幼鼠下肢长骨长度并比较胫骨生长板形态学,免疫组化法测定生长板IGF-1表达量。结果两组IUGR鼠生长板各指标（胫骨长度、股骨长度、胫骨生长板宽度、胫骨增殖期每柱细胞数）和生长板增殖期软骨细胞IGF-1表达显著小于正常对照组（均P〈0.05）,无生长追赶IUGR组更甚。结论IUGR鼠存在生长板局部GH/IGF-1轴受损伴骨生长板纵向生长障碍,无生长追赶者受损更甚,是其导致矮小症的主要机制。     

无机盐和维生素对固体培养槐耳菌丝生长影响的研究

目的研究固体培养基中无机盐和维生素对槐耳菌丝生长的影响。方法以菌丝生长速度与生长状况为衡量指标,设计单因素和正交试验,筛选KH2PO4、Mg SO4和VB1的最佳添加量。结果槐耳菌丝在KH2PO4 3.0 g·L-1、Mg SO4 2.0 g·L-1、VB1 0.009 g·L-1的培养基上生长速度最大、生长状况最优。结论在固体培养基中加入适量的KH2PO4、Mg SO4和VB1,对槐耳菌丝体生长有一定的促进作用。     

内皮细胞生长抑制素的研究现状

从内皮细胞生长抑制素的来源、结构、药理作用、作用机制、制备方法、应用研究等诸方面，综述其最新研究进展。内皮细胞生长抑制素可通过阻断血管内皮细胞的生长及新生血管的形成，抑制肿瘤的生长和转移，成为目前抗肿瘤药物的研究热点。     

不同MEM培养基培养2BS细胞生长情况比较

目的：比较海克隆、日水MEM培养基培养2BS细胞生长过程的细胞贴壁、细胞形态、分裂速度、单层数量等生长情况。方法分别按标准配制日水、海克隆两种MEM培养基,2BS细胞从复苏开始分别用两种培养基进行传代培养。比较细胞生长状况及形态。结果海克隆公司MEM和日水MEM在细胞生长各个方面均无差异,符合人胚肺二倍体细胞的生长需要。结论美国海克隆实验公司的MEM培养基完全支持2BS细胞的生长要求。     

焦磷酸盐、柠檬酸盐对草酸钙结晶形成和凝集的作用

一般认为,尿石形成的前题是结晶尿,但在正常人尿中往往也可见到草酸钙结晶。因此尿中结晶生长和凝集对结石形成更为重要。为研究焦磷酸盐、柠檬酸盐对草酸钙结晶生长和凝集的作用,本文设计了草酸钙结晶生长模型,以分析焦磷酸盐,柠檬酸盐在体外对草酸钙结晶生长,凝集的作用,并对草酸钙结晶生长模型作了研究。     

欧当归(Levisticum officinale Koch)的生物学特性观察研究

本文报导欧当归在我国引种时其幼苗的生长、春季返青、抽茎孕蕾、花茎生长和分枝性状以及开花结实等生物学和生态学特性.     

经验模态分解法与长期生长趋势资料的统计分析

目的:了解长期生长趋势资料统计分析方法的不足,寻找经验模态分解法在生长长期趋势资料分析中的可行性。方法:通过阅读国内外有关生长长期趋势统计分析方法、经验模态相关理论及应用的文献,寻找经验模态分解法理论与生长长期趋势资料分析的结合点,探讨基于经验模态分解法的生长长期趋势资料的分析方法构建的可行性。结果:现有的长期生长趋势统计分析方法不能将遗传和环境因素的作用分离,不能用于客观评价环境因素在生长发育水平的影响。经验模态分解法是目前国际公认的分析非线性、非稳态时间序列数据的最好方法,由于体质状况受遗传和环境因素综合影响,使得长期生长趋势资料呈现非线性、非稳态特征,从理论上讲,经验模态分解法可用于长期生长趋势资料的统计分析。结论:经验模态分解法适合长期生长趋势资料的统计分析,基于经验模态分解法理论创建长期生长趋势统计分析新方法是解决传统统计分析方法诸多不足的一条全新的思路。     

氮芥体外诱导HepG2细胞Gadd和p38 MAPK基因的表达模式

生长抑制和DNA损伤（growth arrest and DNA damage，Gadd）基因是对细胞生长呈负性调节的应激反应基因。DNA损伤因素和生长抑制因素，如烷化剂、紫外线、缺氧和生长阻滞因素等”，是诱导它表达的重要信号。Gadd基因表达的产物及其功能和调节机制还未完全清楚，它的诱导表达可能与激酶的活化、胞内钙离子浓度以及硫醇氧化还原状态等改变激活细胞信号转导通路有关。     

大豆异黄酮对YAC—1细胞和小鼠ESC艾氏肉瘤生长的抑制作用

目的 观察大豆异黄酮对YAC-1细胞及小鼠ESC艾氏肉瘤生长的抑制作用及对免疫功能的影响.方法用MTT法测定大豆异黄酮对YAC-1细胞生长的抑制率和小鼠淋巴细胞转化刺激指数;LDH释放法测定小鼠NK细胞活性;称重观察肿瘤生长和脾指数和胸腺指数.结果金雀异黄素和大豆甙元剂量依赖性抑制YAC-1细胞生长,并随时间延长作用增强;大豆异黄酮50、100、400 mg·kg     

中药的功效源于其自然属性

自然属性是指药物的性味、归经、升降浮沉、形体部位、生长趋势、颜色、质地等等。药物生长的自然环境、生长规律和入药部位,决定着它的自然属性,而这些自然属性又决定着它功效。举例说明,很多中药本身生长在同一种植物上,因为应用的部位不同,它的自然属性就会不同,功效也就随之有所不同。     

晕痛舒胶囊中川芎的鉴别研究

目的：建立晕痛舒胶囊质量标准中川芎的鉴别方法。方法：采用显微鉴别、薄层色谱鉴别及理化鉴别对方中川芎进行定性鉴别。结果：用薄层色谱鉴别,供试品色谱中,在与对照药材色谱相应的位置上显相同颜色的荧光斑点。色谱清晰,附近无杂质斑点干扰,显微鉴别特征虽明显,但是阴性样品有影响,理化鉴别显紫红色,阴性样品也出现相同现象。结论：采用薄层色谱法鉴别晕痛舒胶囊中的川芎,操作简便,专属性强,可更好地控制晕痛舒胶囊的质量。     

龙胆苍耳合剂的制备及薄层色谱鉴别

目的研究龙胆苍耳合剂的制备及质量检测方法。方法采用蒸馏法与水提醇沉法相结合制备龙胆苍耳合剂;采用薄层色谱法对处方中的龙胆草、川芎、黄芩、薄荷进行定性鉴别。结果处方中的龙胆草、川芎、黄芩、薄荷均能用薄层色谱法检测。结论蒸馏法与水提醇沉法结合制备龙胆苍耳合剂工艺简便、可靠;薄层色谱法用于龙胆苍耳合剂的定性检测准确、灵敏。     

Antibacterial activity and interactions of plant essential oil combinations against Gram-positive and Gram-negative bacteria

The aim of this study was to compare the antibacterial effects of several essential oils (EOs) alone and in combination against different Gram-positive and Gram-negative bacteria associated with food products. Parsley, lovage, basil, and thyme EOs, as well as their mixtures (1:1, v/v), were tested against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium. The inhibitory effects ranged from strong (thyme EO against E. coli) to no inhibition (parsley EO against P. aeruginosa). Thyme EO exhibited strong (against E. coli), moderate (against S. typhimurium and B. cereus), or mild inhibitory effects (against P. aeruginosa and S. aureus), and basil EO showed mild (against E. coli and B. cereus) or no inhibitory effects (against S. typhimurium, P. aeruginosa, and S. aureus). Parsley and lovage EOs revealed no inhibitory effects against all tested strains. Combinations of lovage/thyme and basil/thyme EOs displayed antagonistic effects against all bacteria, parsley/thyme EOs against B. cereus, S. aureus, P. aeruginosa, and E. coli, and lovage/basil EOs against B. cereus and E. coli. Combinations of parsley/lovage and parsley/basil EOs exhibited indifferent effects against all bacteria. The combination of lovage/basil EO showed indifferent effect against S. aureus, P. aeruginosa, and S. typhimurium, and the combination parsley/thyme EO against S. typhimurium. Thyme EO has the highest percentage yield and antibacterial potential from all tested formulations; its combination with parsley, lovage, and basil EOs determines a reduction of its antibacterial activity. Hence, it is recommended to be used alone as the antibacterial agent.     

桃仁膝康丸的薄层色谱研究

目的建立医院制剂桃仁膝康丸的质量控制方法。方法采用薄层色谱法对处方中三七、白芍、当归和川芎进行定性鉴别。结果薄层色谱图中斑点显色清晰,阴性对照无干扰。结论所建立的方法重现性好,可用于桃仁膝康丸的质量控制。     

川芎嗪纳米喷雾剂对大鼠腹腔粘连相关因子和纤溶系统影响的实验研究

目的探讨川芎嗪纳米喷雾剂对大鼠腹腔粘连相关因子和纤溶系统的影响。方法将SD大鼠随机分为空白组、模型组、透明质酸钠组、川芎嗪纳米喷雾剂高、中、低剂量组、聚乳酸空白纳米组、川芎嗪原料组、川芎提取物组共9组。以锉刀法进行造模,分别于术后1周、2周收集大鼠腹腔液,ELISA法测定腹腔液中相关因子TGF-β1、FN、CTGF的含量,以及纤溶系统PAI和t-PA的含量。结果川芎嗪纳米喷雾剂有改善粘连相关因子TGF-β1、FN、CTGF升高的作用;与粘连模型组比较,抑制了PAI活性的增强,相对增强了t-PA的活性作用,且川芎嗪纳米喷雾剂存在一定的量效关系。结论川芎嗪纳米喷雾剂抗腹腔粘连的作用机制可能与抑制了粘连所致的TGF-β1、FN、CTGF的升高以及调节了t-PA、PAI之间的平衡相关。     

Cytotoxicity,antioxidant activity and an effect on CYP3A4 and CYP2D6 of Mutellina purpurea L. extracts

Mutellina purpurea is an aromatic Apiaceae plant known as Alpine lovage. Its polar extracts consist of phenolic acids, tannins and flavonoids. The cytotoxic effect of methanolic and aqueous extracts from M. purpurea was studied on the most frequently used cell lines: HeLa and BHK-21. Taking into account that the natural products are often used with other medicines there is a risk of reciprocal interaction on the metabolic level. Thus, the influence of M. purpurea extracts was investigated on the activity of CYP2D6 and CYP3A4, which are the most important P450 isoenzymes from the pharmacological and toxicological points of view. Additionally, because M. purpurea contains phenolic compounds, the antioxidative properties of this plant extracts were also studied and compared.     

Influence of extraction methods on the composition of essential oils

The aim of this work is to demonstrate our results on comparison of composition of essential oil fractions obtained by traditional steam distillation and supercritical fluid extraction. The plant materials for the various extraction methods were selected from the Lamiaceae, Apiaceae and Asteraceae families. For the supercritical fluid extraction (SFE) carbon dioxide was used as supercritical solvent. The extracts were collected by stage wise precipitation in two separators. The waxy product and extract rich in essential oil were collected in the 1st and in the 2nd separator respectively. The traditional water steam distillation (SD) was carried out in the special apparatus of the Hungarian Pharmacopoea (7th ed.). GC analysis was carried out on capillary silica fused columns coated with DB-1701 and the specific chiral columns coated with Rt-beta DEX m or Rt-beta DEX sm. Comparing the composition of steam distilled oils with that of volatile SFE fractions the following general characteristics were established. The SFE fractions were richer in monoterpene-esters and poorer in alcohols than the traditional essential oils (clary sage, lavander, moldavian dragonhead). Regarding the distribution of the monoterpene and sesquiterpene compounds, the SFE fractions contained sesquiterpenes in higher percentage than the distilled oils (Salvia fruticosa). Furthermore, the proportion of sesquiterpenes increased in SFE fractions collected successively with time (Salvia officinalis) similar to the ratio of oxygenated monoterpenes to monoterpene hydrocarbons (Rosmarinus officinalis). The phtalides of lovage (Satureja hortensis) did not show regular change during the supercritical extraction. In other cases it was verified that part of the mono- and sesquiterpenes were present originally in bound form (glycosides) in plants. Thus they appeared only in essential oil fractions after previous acidic treatment (Thymus, Origanum, Satureja species). During the super-critical extraction the azulenogene sesquiterpene lactones did not transform to azulenes (chamomile, yarrow), but SFE fractions of some Asteraceae plants contained sesquiterpene-gamma-lactones of unchanged structure.     

Change in annexin A3 expression by regulatory factors of hepatocyte growth in primary cultured rat hepatocytes

We have recently reported that annexin (Anx) A3 expression is necessary for hepatocyte growth in cultured rat hepatocytes seeded at half the subconfluent density on collagen. In the present study, we investigated the effects of various regulatory factors of hepatocyte growth on AnxA3 expression. AnxA3 expression was significantly reduced in hepatocytes cultured under various growth inhibitory conditions such as presence of dexamethasone, culture at subconfluent cell density, and on EHS-Matrigel and lactose-carrying styrene polymer. On the other hand, hepatocyte growth factor and epidermal growth factor, stimulators of hepatocyte growth, significantly increased AnxA3 expression in hepatocytes cultured on EHS-Matrigel. These results show close correlation between known stimulatory or inhibitory actions of various factors to hepatocyte growth and increase or decrease in AnxA3 expression, and suggest the involvement of AnxA3 in their regulation of hepatocyte growth.     

Antineoplastic action of growth hormone-releasing hormone (GHRH) antagonists

Some of the antagonists of growth hormone-releasing hormone (GHRH) are able to inhibit the growth of various experimental human cancers. The antitumor effects of first antagonists seemed to be dependent mainly on the disruption of pituitary secretion of growth hormone (GH), followed by the reduction in the levels of circulating insulin-like growth factor (IGF)-1, an important growth factor for cancer cells. It seems obvious, that growth hormone deficiency (GHD) induced by GHRH antagonists with all its complications, could limit the beneficial effects of GHRH antagonists therapy, and decrease patients' quality of life. The discovery of local autocrine/paracrine production of GHRH and other related growth factors in many tumoral tissues, in combination with the wide expression of GHRH receptors on cancer cells, directed the research to the synthesis of more potent GHRH antagonists. These compounds exert strong inhibitory effects directly on tumor growth, with scarce endocrine action. The receptor-mediated mechanisms comprise complex and still not completely understood effects on intracellular signaling pathways that are strictly related to human tumorigenesis. This review summarizes recent patents and latest observations on the antineoplastic role of GHRH antagonists in human tumors with emphasis on potential therapeutic applications in clinical oncology.     

SIRT1 targeted by miR-211-5p regulated proliferation and apoptosis of Dex-treated growth plate chondrocytes via mediating SOX2

Dexamethasone (Dex) is reported to cause bone growth retardation in children, which is associated with the increased apoptosis and decreased proliferation of growth plate chondrocytes. Sirtuin 1 (SIRT1) plays an important role in chondrocyte function and homeostasis. Thus, we further explored the regulatory mechanism of SIRT1 in Dex-induced growth plate chondrocyte dysfunction. SIRT1 expression was detected in Dex-treated growth plate chondrocytes using RT-qPCR and western blot assay. The modulation of SIRT1 on SOX2 expression was evaluated. Besides, we identified that SIRT1 was targeted by miR-211-5p using TargetScan and RNA pull-down assay. A loss-of-function assay was performed to evaluate the effects of miR-211-5p on Dex-induced growth plate chondrocyte dysfunction in vitro and in vivo. We found that SIRT1 was downregulated in Dex-treated growth plate chondrocytes. The expression of SOX2 was upregulated by overexpression SIRT1. Meanwhile, downregulation of SOX2 weakened the positive function of SIRT1 overexpression on Dex-induced growth plate chondrocytes dysfunction. Subsequently, we confirmed that SIRT1 was targeted by miR-211-5p. MiR-211-5p inhibitor increased the expression levels of SIRT1 and SOX2, and restored the Dex-treated growth plate chondrocyte function. Animal assays further demonstrated that the effects of miR-211-5p on the growth plate chondrogenesis. In conclusion, our data suggest that SIRT1 exerts a protective effect on growth plate chondrocyte under Dex stimulation. MiR-211-5p/SIRT1/SOX2 axis regulates the process of Dex-inhibited growth plate chondrogenesis.