Endodontic infection control routines among general dental practitioners in Sweden and Norway: a questionnaire survey

Objective: The purpose of this study was to investigate endodontic infection prevention and control routines among general dental practitioners in Sweden and Norway.

Materials and methods: A questionnaire was sent by email to 1384 general dental practitioners employed in Sweden and Norway. The participants were asked questions concerning different aspects of infection prevention and control during endodontic treatment; use of rubber dam, sealing of rubber dam, antibacterial solutions, and use of hand disinfectant and gloves.

Results: The response rate was 61.4% (n: 819). 96.9% reported routinely using rubber dam during endodontic treatment. 88.3% reported always, or sometimes, sealing the area between rubber dam and tooth. Most disinfected the endodontic operative field, but the antibacterial solutions used varied. 11.9% did not use gloves at all during treatment, and 10.5% did not use hand disinfectant during treatment.

Conclusions: Most of the general dental practitioners took measures to establish and maintain aseptics during endodontic treatment, which infers an awareness of the importance of endodontic infection prevention and control. But the results were self-reported and there may be a gap between claimed and actual behaviour. Further studies using observation methodologies are needed to assess how infection control routines are performed in everyday clinical practice.     

A study of endodontic treatment carried out in dental practice within the UK

AIM: The purpose of this study was to gather both qualitative and quantitative information on the nature of root canal treatment carried out by a group of dentists working within the United Kingdom. METHODOLOGY: A two-part questionnaire was posted to 720 dentists who graduated from the Dental School, Cardiff, Wales, UK. The first part requested basic information regarding age, year of qualification, field of practice, etc. The second part consisted of 15 questions on endodontic practice and root canal treatment. RESULTS: The response rate was 41.5%. Two hundred and ninety-nine questionnaires contained useful information. The majority of practitioners did not use rubber dam during root canal treatment. The vast majority (89%) exposed a radiograph with an instrument of known length in situ to gauge the 'working length', a small number relied upon tactile sensation. Most practitioners used local anaesthetic solution as an irrigant during instrumentation of the root canal. A wide variety of instruments were used for root canal treatment; a stepback technique was preferred by almost half the practitioners. Antiseptic solution was preferred as an interappointment dressing. More than half of the respondents used laterally condensed gutta-percha to obturate root canals in anterior teeth but only one-third used the same technique in posterior teeth. Less than half the respondents exposed a radiograph to check the fit of the master point prior to obturation. Two-thirds of practitioners used a zinc oxide based material as their root canal sealer. Three-quarters of the practitioners exposed a post obturation radiograph. CONCLUSIONS: The results of this study suggest that although some dentists are using the techniques taught during their undergraduate careers, a large percentage now use techniques with no evidence of clinical effectiveness.     

The use of rubber dam in the UK. A survey

The purpose of this survey was to determine the frequency of use of rubber dam isolation in the United Kingdom for both operative and endodontic procedures. Questionnaires were sent to 1800 dentists throughout the country, with 1008 returns (56%). Most replies were from dentists active in general dental practice. The use of rubber dam is largely neglected and more than 70% of all dentists surveyed did not utilise rubber dam for any procedure whatsoever.     

Attitudes and use of rubber dam by Irish general dental practitioners

AIM: To investigate the attitudes towards and use of rubber dam by Irish general dental practitioners. METHODOLOGY: A pre-piloted questionnaire was distributed amongst a group of 600 dentists randomly selected from the Irish Register of Dentists. Replies from dentists working in specialist practice or the hospital dental service were excluded. Dentists were surveyed in relation to their use of rubber dam during a variety of operative and root canal treatments, as well as their attitudes to the use of rubber dam in dental practice. RESULTS: A total of 300 replies were considered from a total of 324 that were received. Seventy-seven per cent of respondents (n=231) worked in general dental practice and 23% (n=69) worked in the Irish Health Board/Community Dental Service. Rubber dam was 'never' used by 77% of respondents (n=228) when placing amalgam restorations in posterior teeth, 52% (n=147) when placing composite restorations in posterior teeth, and 59% (n=177) when placing composite restorations in anterior teeth. Rubber dam was 'never' used by 39% of respondents (n=114) when performing root canal treatment on anterior teeth; 32% (n = 84) when performing root canal treatment on premolar teeth; and 26% (n=51) when performing root canal treatment on molar teeth. Fifty-seven per cent (n=171) considered rubber dam 'cumbersome and difficult to apply', and 41% (n=123) considered throat pack 'as good a prevention against inhalation of endodontic instruments as rubber dam'. CONCLUSIONS: Whilst rubber dam is used more frequently for root canal treatment than operative treatment, its use is limited. This presents quality issues, as well as medico-legal and safety concerns for both the profession and patients.     

Rubber dam use during root canal treatment: Findings from The Dental Practice-Based Research Network

Background

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The Dental Practice-Based Research Network (DPBRN) provided a means to investigate whether certain procedures were performed routinely. The authors conducted a study to quantify rubber dam use during root canal treatment (RCT) among general dentists and to test the hypothesis that certain dentist or practice characteristics were associated with rubber dam use.MethodsDPBRN practitioner-investigators (P-Is) answered a questionnaire that included items about rubber dam use and other forms of isolation during RCT. DPBRN enrollment questionnaire data provided information regarding practitioner and practice characteristics.ResultsA total of 729 (74 percent) of 991 P-Is responded; 524 were general dentists who reported providing at least some RCTs and reported the percentage of RCTs for which they used a rubber dam. Of these 524 P-Is, 44 percent used a rubber dam for all RCTs, 24 percent used it for 51 to 99 percent of RCTs, 17 percent used it for 1 to 50 percent of RCTs, and 15 percent never used it during RCT. Usage varied significantly by geographic region and practice type. The use of cotton rolls and other forms of isolation also was reported.ConclusionsSimilar to other reports in the literature, not all DPBRN general dentists used a rubber dam during RCT.Clinical ImplicationsBecause the clinical reference standard is to use a rubber dam during RCT, increasing its use may be important.     

The 5-Year Survival Rate of Nonsurgical Endodontic Treatment: A Population-based Cohort Study in Korea

IntroductionThe purpose of the current population-based study was to analyze the 5-year survival rate of teeth that received nonsurgical endodontic treatment. Specifically, the variables affecting the 5-year survival rates of endodontically treated teeth were analyzed.MethodsThe present study included all endodontically treated teeth reported in 2010 in the National Health Insurance Cohort database of the Republic of Korea. By tracing the dental records of the sample to the end of 2015, the 5-year survival rates of the initial root canal treatment (RCT) and re-RCT were analyzed. Gender, age, institution type, diagnosis, arch type, tooth type, number of visits, and rubber dam usage were included in the analyses as confounding variables.ResultsIn total, 2,866,749 teeth received initial RCT, and 173,429 teeth received re-RCT. Five-year survival rates were 90.85% and 88.42%, respectively. The survival rate of teeth that received initial RCT was significantly higher than that of those that received re-RCT. Specifically, re-RCT showed a significantly higher rate of extraction within 12 months than during other intervals. Furthermore, the following characteristics significantly positively affected the 5-year tooth survival rate: being female, <20 years of age, hospital as the institution type, mandibular teeth, anterior teeth, initial RCT, and use of rubber dam.ConclusionsBecause of its high 5-year survival rate, endodontic treatment is an effective method for maintaining natural teeth.     

Resumption of Endodontic Practices in COVID-19 Hardest-Hit Area of China: A Web-based Survey

IntroductionThe purpose of this study was to investigate the resumption of endodontic practices in Hubei Province, China 1 month after the end of the lockdown.MethodsA Web-based survey was sent to 1069 active endodontic specialists and general dentists who provided endodontic care in Hubei Province from May 7 to May 9, 2020. The survey consisted of 18 questions on demographics, the current situation of endodontic practice for the participants, and concerns regarding contracting or spreading the virus in newly opened endodontic practices.ResultsA total of 322 participants completed the survey. Most respondents (62%) were from Wuhan. Almost 83% of the respondents have resumed their endodontic practice partially or fully. Most respondents in practice (99%) would take measures to screen patients before treatment including paid screening measures. More than 93% of the respondents reported having taken special measures for routine endodontic treatment at this stage, with the most common measure taken being wearing an N95 mask. The rubber dam was recognized as efficient in preventing infection by most respondents. Many respondents were concerned about contracting coronavirus disease 2019 (COVID-19) as a result of routine endodontic practice at this stage, and respondents with more than 11 years of endodontic experience were significantly less concerned about infection compared with those with fewer than 5 years of experience (P < .05).ConclusionsThere is a fear of contracting/spreading COVID-19 among endodontic clinicians, the fear is negatively associated with years of practice, and most clinicians believe that rubber dam isolation can protect them and their patients from COVID-19.     

Treatment preferences of deep carious lesions in mature teeth: Questionnaire study among dentists in Northern Norway

Abstract

Objective. To investigate preferred treatment methods of deep carious lesions in mature permanent teeth among dentists in Northern Norway. Materials and methods. The survey included all 437 general dental practitioners in Northern Norway working in the private or the public dental health service. The participants received an invitation with a radiograph and a clinical picture of a deep carious lesion in a mature permanent tooth and a questionnaire asking about demographic characteristics and their preferred treatment methods related to different clinical deep caries diagnoses. Results. The response rate was 56%. There was an over-representation of publicly employed dentists among the respondents; otherwise no systematic bias related to non-responders was detected. In the absence of carious exposure and no symptoms, total caries excavation was the preferred treatment method (49%) followed by stepwise excavation (45%). In cases of deep caries and no exposure with symptoms, the preferences were equally distributed between root canal treatment (39%) and stepwise excavation (38%). In the presence of carious exposure but no symptoms, the preferred treatment method was direct pulp capping (51%) and in carious exposure with symptoms root canal treatment was the preferred treatment method (91%). Conclusions. There is no uniform treatment method of deep carious lesions among dentists in Northern Norway.     

Ten-year follow-up on adoption of endodontic technology and clinical guidelines amongst Danish general dental practitioners

Objective: The aim of this study was to re-assess the adoption of certain endodontic technology and central treatment principles of root canal treatments as advocated by guidelines presented by the European Society of Endodontology.

Material and methods: The questionnaire included the same questions in 2003 and 2013. The general dental practitioners (GDPs) anonymously reported how frequent (‘often’, ‘occasionally’, and ‘never’) they used certain endodontic technology and adhered to central treatment principles. The statistical analyses were performed using Chi-squared test and Goodman–Kruskal’s γ-coefficient as an association measure.

Results: The overall response rate of the 2013 group was 46.5% (n?=?531). The frequencies of GDPs reporting often use of rubber dam, apex locator and rotary NiTi instruments were significantly higher (p?p?p?=?.601) and time since graduation (p?=?.361), and the cluster analyses revealed the neglected use of rubber dam to be associated with no established postoperative recall system.

Conclusions: After 10 years, there was a higher frequency of GDPs who had adopted certain endodontic technologies. However, progress towards high-quality root canal treatment might be obstructed as the majority of GDPs avoids consistent use of rubber dam, and routinely neglects recalls for postoperative controls of their endodontic treatments.     

Do dental educators need to improve their approach to teaching rubber dam use?

Most dentists are educated in rubber dam use in dental school, but there is often disparity between what is taught for various restorative procedures and what is practiced in the private sector. It is a common, although undocumented, belief that few practicing dentists routinely use rubber dam isolation. This study repeated a survey conducted in 1985 evaluating U.S. general dentists' attitudes toward rubber dam usage to see if improvement is needed in current dental educators' approach to this topic. Four hundred dentists were selected randomly from ten major geographically diverse cities using the website YellowPages.com. Each was mailed a letter requesting survey participation, which included a pre-stamped, pre-addressed postcard with the survey printed on the back. The target population, general dentists, returned 164 surveys (41 percent). Their responses can be summarized as follows: 71 percent do amalgams-of those, 53 percent never use a rubber dam whereas 12 percent always use a rubber dam; 100 percent do anterior direct resin composites-of those, 45 percent never use a rubber dam whereas 17 percent always use a rubber dam; 98 percent do posterior direct resin composites-of those, 39 percent never use a rubber dam and 18 percent always use a rubber dam; and 78 percent do endodontic procedures-of those, 11 percent never use a rubber dam whereas 58 percent always use a rubber dam. Most (74 percent) felt that their dental school rubber dam training was adequate; 42 percent felt that its use has an effect on the quality of restorative dentistry. Their most common reasons for not using a dam were the following: inconvenience (40 percent); unnecessary (28 percent); other (12 percent); patient refusal (11 percent); and time (9 percent). No respondent indicated that "cost" was a reason for not using rubber dams. This study indicates that many general dentists in this country continue to ignore the rubber dam for many restorative and some endodontic procedures. It indicates that predoctoral dental educators need to look for opportunities for improvement to reduce the discrepancy between what is taught and the general practice of dentistry.     

Dental practice in Northern Ireland, UK

Abstract A postal survey concerning clinical practice by general practitioners in Northern Ireland U.K. had a response rate of 71.2%. There were little differences in practice between chose who had graduated at the Dental School in Belfast (70%) and those who qualified elsewhere. Older dentists appeal- to undertake less ambitious dentistry and while this might be a reflection of their training, there were some practices of younger dentists such as not using a rubber dam for endodontic treatment which was certainly not taught to them at Dental School.     

The attitudes of undergraduate dental students to the use of the rubber dam

ABSTRACT: The majority of dental schools teach that use of the rubber dam is mandatory for certain procedures, e.g., endodontic therapy and adhesive dentistry. Despite this, many dentists perceive use of the rubber dam as a hindrance to dental treatment in adults and children. PURPOSE: This study sought to evaluate the attitude of undergraduate students towards the use of the rubber dam and elucidate if the negativity towards its use is evident in undergraduate clinics. METHODS: A structured questionnaire was developed, which sought to establish current perceptions of the use of the rubber dam in adults and children, and circulated to the current fourth and final year clinical undergraduate class in the Dublin Dental Hospital. RESULTS: Isolation was identified as the main advantage of using a rubber dam and difficulty to place the main disadvantage. Some 98.5% of students believed they had received adequate training in rubber dam use for adults. While 72% of students were confident in placing a rubber dam for children, 38% felt that more training was necessary. The most commonly cited difficulty in placing a rubber dam was tight contacts. Most students are confident regarding which clamps to use. The majority of students (61%) believe that patients, especially children, prefer treatment without rubber dam isolation and that adequate isolation can be achieved without using a rubber dam. The average time spent placing the rubber dam was eight minutes for children and five minutes for adults. The students are more predisposed to using the rubber dam on adults than children for the same procedure. CONCLUSIONS: Undergraduate students are not convinced that the use of a rubber dam is effective and efficient except for endodontic therapy. Students already believe that patients, both adults and children, would prefer their treatment to be conducted without a rubber dam. Teaching efficient methods of placing the rubber dam may improve students' confidence and reduce placement time so that the students will be more likely to use the rubber dam after graduation.     

Influence of the coronal restoration on the outcome of endodontically treated teeth

Abstract

Objective: The tooth weakens due to removal of hard tissue during an endodontic procedure. Many dentists find it difficult to choose between different coronal restorations after root canal treatment (RCT). Studies show that the coronal restoration may affect the endodontic prognosis. This student-based study had three aims. (1) Examine the choice of coronal restoration of endodontically treated teeth at a Scandinavian dental school, (2) examine the survival of these restorations and (3) evaluate the influence of the coronal restoration on the outcome of the RCT.

Material and methods: Radiographic and clinical examination was performed on 127 posterior teeth. The quality of the root canal treatment and the periapical status (PAI-index) were evaluated.

Results: 43.8% of the teeth were restored with an indirect coronal restoration and 47.2% with a direct coronal restoration. The period from finished root canal treatment until placement of a permanent coronal restoration was significantly longer for an indirect restoration than a direct restoration. The teeth treated with a PAI score of 1 and 2 following pulpectomy, necrotic pulp treatment and endodontic retreatment was 93.8%, 82.6% and 69.4%, respectively.

Conclusion: There was no significant association between choice of coronal restoration and PAI-score.     

Use of rubber dam and irrigant selection in UK general dental practice

AIM: To evaluate factors which influence rubber dam use and irrigant selection in UK National Health Service (NHS) endodontics. METHODOLOGY: A postal survey was conducted amongst two age cohorts of dentists, representing all of the 1970-73 (older) and 1990-93 (younger) graduates of two northern English dental schools (n = 643). Key and supplementary questions were posed on levels of rubber dam use, irrigant selection, and factors influencing practice in NHS endodontics. After manual checking, validated (dual) entry of responses was made to a flat ASCII data file before analysis with SPSS software. The threshold for statistical significance was set at the 95% probability level. RESULTS: Eighty-five per cent of the valid sample responded to the questionnaire. Regardless of age and qualifying school, less than one-fifth of dentists always or frequently used rubber dam, whilst 60% never used it. Qualifying school had a significant influence on rubber dam use, whilst age had a variable influence. Major disincentives to the use of rubber dam included the perception that patients do not like it, that the NHS fee was inadequate to justify its use, that it took too long to apply, and that dentists had received inadequate training. Frequent users of rubber dam were significantly less likely to cite these disincentives than nonusers. Overall, local anaesthetic solution was the most common endodontic irrigant. Irrigant choice was strongly linked to rubber dam use, and to graduation cohort. Seventy-one per cent of rubber dam users irrigated with sodium hypochlorite, compared with only 38% of nonusers. This pattern was reversed for local anaesthetic irrigation. Younger graduates were significantly more likely to irrigate with local anaesthetic solution than their older counterparts, and the younger graduates of one school showed a highly significant increase in the use of chlorhexidine. CONCLUSIONS: 1) The majority of UK Health Service dentists never use rubber dam isolation in endodontic treatment. 2) Qualifying school has a significant impact on rubber dam use, and irrigant selection. 3) Use of rubber dam has a significant association with irrigant choice in endodontics.     

Accidental ingestion and aspiration of root canal instruments and other dental foreign bodies in a French population

AIM: To determine the incidence of aspiration and ingestion of endodontic instruments in France during root canal treatment without using rubber dam. METHODOLOGY: Data was provided by two insurance companies representing 24,651 French general dentists over 11 years. The type and number of accidents per year, the number of dental items involved and the percentage of occurrence of either aspiration or ingestion were reported. The incidence of accidental aspiration or ingestion was calculated. The need for hospitalization to remove the endodontic instruments and other dental items was reported and compared using chi square tests. RESULTS: One endodontic instrument was aspirated and 57 were ingested. Forty-three other dental items were aspirated and 409 were ingested. For the endodontic instruments: the incidence of aspiration was 0.001 per 100,000 root canal treatments and the incidence of ingestion was 0.12 per 100,000 root canal treatments. The aspirated endodontic instruments and dental items required statistically more frequent hospitalization than the ingested items (P < 0.0001). The endodontic instruments did not require more frequent hospitalization than other dental items when aspirated (ns) and when ingested (ns). No fatal outcome was reported. CONCLUSION: The incidence of ingestion or aspiration of endodontic instruments was low even thought most general practitioners do not routinely use rubber dam. Use of rubber dam by general practitioners for endodontic procedures should be encouraged by stressing its advantages rather than the fear factor of accidents.     

Periapical status of root-filled teeth in Norwegian children and adolescents

Objective. To study the periapical status of root-filled permanent teeth of 9–17-year-olds in Møre & Romsdal county, Norway. Materials and methods. All permanent teeth with endodontic treatment in patients born in 1994–2001 were identified in the dental records of the Public Dental Service. The data collected consisted of chart entries and radiographs. Of 1182 teeth, 174 teeth in 155 patients met further inclusion criteria, i.e. completed endodontic treatment and a follow-up with a readable radiograph of at least 1 year. Periapical status was assessed with the Periapical Index (PAI) and the technical quality of the root fillings was also quantified on a 4-grade visual scale. Results. Apical periodontitis was found on follow-up radiographs in 25% of all teeth and in 48% of the teeth with pre-operative apical periodontitis. Forty-two per cent of root fillings were of adequate technical quality. Adequate technical quality of the root filling was significantly correlated with radiographic success. Conclusions. About one fourth of all root-filled teeth and almost half of the teeth with pre-operative apical periodontitis showed clear signs of the disease at recall.     

Endodontic follow-up practices,sources of knowledge,and self-assessed treatment outcome among general dental practitioners in Sweden and Norway

Abstract

Objective: To reduce the gap between what can be achieved in endodontic treatments and the observed treatment outcome among general dental practitioners, the present study set out to assess the status of the endodontic practices as regards to knowledge and self-assessed skills among general dental practitioners in Sweden and Norway.

Material and method: The questionnaire was sent to 1384 general dental practitioners. It contained questions regarding access to continuing education in endodontics, sources of knowledge for clinical management of patients, post-operative follow-ups, self-assessed success-rate, and the initial diagnosis impact on the outcome of endodontic treatments.

Results: The response rate was 61.4%. Almost half estimated their endodontic success-rate to be 90%. About two-thirds of the respondents did not know, or did not believe, that the initial diagnosis could affect the outcome of their endodontic treatments. Respondents who did not believe the diagnosis could impact the outcome were more likely to estimate their success rate as the highest (p<.001). Less than half performed post-operative follow-ups a year after treatment. A third of the respondents had not attended any continuing endodontic education.

Conclusion: Dentists who do not receive regular feedback on their treatments may lack insight into their own shortcomings. If this is combined with insufficient knowledge and understanding it may result in sub-par endodontic treatments being performed. It is important to have reliable ways to communicate current endodontic knowledge and to establish robust methods that may help dentists accurately assess their own performance in endodontics.     

Compliance with Key Practices of Root Canal Treatment Varies by the Reward System Applied in Public Dental Services

IntroductionTo assess clinical practices in root canal treatments (RCTs) performed by general dental practitioners under 2 different reward schemes applied in public dental services.MethodsThis study used a retrospective design with tooth as the observation unit. The data included all teeth (n = 547) with nonsurgical primary RCT completed in 2016. Electronic documents included treatment details and radiographs. RCT assessment covered 4 key items: taking pre- and postoperative radiographs, using a rubber dam, measuring working length. Assessed dichotomies indicated whether practices were adequate. Dentists’ reward schemes were “salaried” and “fee-for-service.” Chi squared tests analyzed frequency differences.ResultsRCTs formed 2 groups by the reward scheme: 305 RCTs were performed by salaried dentists and 242 by fee-for-service dentists. Preoperative radiographs were diagnosable for 76.1% and postoperative radiographs, for 95.1% of all RCTs. Rubber dam use was documented for 28.9% of the RCTs, more frequently when performed by salaried than by fee-for-service dentists (43.9% vs 9.9%, P < .001). Working length measurement was documented for 72.9% of the RCTs, more frequently for RCTs performed by salaried than by fee-for-service dentists (85.2% vs 57.4%, P < .001). All 4 key items were assessed as adequate in 19.0% of all RCTs, more frequently when performed by salaried than by fee-for-service dentists (29.5% vs 5.8%, P < .001).ConclusionsDeficiencies in RCTs, particularly underuse of rubber dams call for further research to understand the reasons for noncompliance with good clinical practice guidelines.     

Knowledge and competence in temporomandibular disorders among Swedish general dental practitioners and dental hygienists

Objective: The aim of this study was to investigate knowledge and competence in temporomandibular disorders (TMD) among dentists and dental hygienists working in the public dental service (PDS) in Sweden.

Materials and methods: The study population comprised all general dentists (n?=?110) and dental hygienists (n?=?80) working in the PDS in two Swedish counties: Kronoberg (K) and Blekinge (B). The participants filled out a questionnaire comprised of 15 questions.

Results: The results of these questions are presented. The overall response rate for the general dentists was 87%, while the rate for the dental hygienists was 71%. Statistically significant differences between the general dentists in the two counties were found regarding the following: education in the field of TMD over the last 5 years (K: 37%, B: 73%), evaluation of occlusion when examining patients with suspected TMD (‘always’: K: 61%, B: 82%), and a desire for consultation of the OFP (orofacial pain)/TMD specialist by telephone (K: 71%, B: 44%). Regarding the dental hygienists, there was a statistically significant difference concerning the use of the treatment modality ‘reassurance’ (K: 41%, B: 7%).

Conclusions: The majority of the dental care providers in both counties – irrespective of professional category – had a positive attitude towards patients with TMD. Knowledge and competence in the field are sparse and require postgraduate education. There is a great need of an OFP/TMD specialist for more complicated patients and a need to implement updated knowledge and competence in the PDS in these two counties.     

Maintenance of the aseptic working field during endodontic treatment

Objective: The aim of this clinical quality study was to determine whether the aseptic working field is maintained during the endodontic procedure.

Materials and methods: Bacterial samples were collected from the rubber dam of 27 patients during endodontic treatment performed by postgraduate students at the Department of Endodontics, University of Oslo. A bacterial sample was first obtained immediately after disinfection of the working field (A), and the second sample was collected just before obturation or dressing with calcium hydroxide cement (B). Aerobic cultivation technique and PCR were used for detection of bacterial growth and species.

Results: All samples were negative on culturing except in one case, which showed positive results with cultivation in both sample A and B. Specie detected with cultivation technique were Streptococcus mitis. With PCR technique, 6 samples in 5 patients (11%), showed positive results. Species detected with PCR technique were Bacteroidales spp. Propionibacterium spp., Bacteroidetes spp., Prevotella nigrescens, Haemophilus parainfluenzae, Neisseria elongata, Alloprevotella tannerae, Capnocytophaga granulosa, Cardiobacterium hominis, Fusobacterium nucleatum and Streptococcus mitis.

Conclusion: The present study showed that an aseptic working field was maintained throughout the endodontic procedure in 81% (22/27) of the cases after disinfection of the rubber dam.