Relationship between intensities of skin test reactions to glass-fibres and chemical irritants

A total of 98 workers at a glass-wool factory were patch tested with glass-fibres and six chemical irritants. A rubbing test with the fibres, a Trafuril test and provocation of dermographism were also performed. The subjects with strong patch test reactions to one quality of fibres also reacted strongly to the other types of fibres but did not show an increased sensitivity to other tests. The subjects with intense skin reactions to rubbing with the fibres showed an increased skin reactivity to Trafuril. A tendency towards increased reactivity to the patch tests with fibres was found in persons with fair skin and blue eyes.     

人型支原体对8种药物的敏感性及喹诺酮类药物耐药基因gyrA突变的研究

目的：检测人型支原体(Mh)对8种药物的敏感性，并检测喹诺酮类药物耐药株gyrA基因突变情况。方法：应用微量肉汤稀释法检测Mh对8种药物的敏感性，用PCR和DNA测序及序列比较检测基因突变。结果：大环内酯类药物中只有交沙霉素抗Mh活性较强，MIC50和MIC90分别为0．25μg／ml和0．5μg／ml，四环素类药物中多西环素的抗Mh活性优于四环素，MIC50仅为0．06μg／ml，MIC90仅为1μg／ml；喹诺酮类药物环丙沙星和氧氟沙星的抗Mh活性较强。基因序列分析表明gyrA基因碱基113位C到T的突变为有义突变，导致所编码的83位丝氨酸被亮氨酸替代，其余均为同义突变。结论：8种药物中以交沙霉素、多西环素、环丙沙星和氧氟沙星的抗Mh的活性较强；gyrA基因113位碱基C到T的突变与Mh耐喹诺酮类药物密切相关。