Treatment of 60 Cases of Prolapse of Lumbar Intervertebral Disc by Electroacupuncture

目的观察电针治疗腰椎间盘突出症的临床疗效.方法运用电针辨证施治治疗腰椎间盘突出症患者60例,并临床随访6个月.结果近期显效40例,好转13例,总有效率为88.3%;远期疗效其中显效45例,好转10例,总有效率为91.7%.结论电针治疗腰椎间盘突出症近远期疗效好,疗效稳定,且方法安全有效,无毒副作用.     

Treatment of 56 Cases of Pain of Supra-orbital Bone by Puncturing Siguan Acupoints

Pain of supra-orbital bone is very common in clinic The author achieved significant effect in treating 56 cases of pain of supra-orbital bone by puncturing Siguan （four gates） points. The report is as follows.     

Treatment of 32 Cases of Meniere Disease with Acupuncture

采用针刺法治疗梅尼埃病32例,穴位用百会、风府,双侧风池、肾俞、太溪、悬钟,结果8例完全控制,12例基本控制,9例部分控制,3例未控制,总有效率为90.6%.     

Survey of Research on Acupuncture Treatment of Peptic Ulcer

回顾分析了近10年针灸治疗消化性溃疡的-临床选穴研究概况，发现临床治疗消化性溃疡以多穴配伍为主，多穴中以脾胃之俞募配穴及脾胃之俞募穴配下合穴为主。表明脾胃之背俞穴、募穴及下合穴对治疗消化性溃疡具有重要作用。     

Treatment of 68 Cases of Epigastric Pain with Acupucnture

Acute and chronic gastritis, gastric and duodenal globular ulcer, and antral gastritis are commonly encountered diseases of digestive system, and epigastric pain is their main symptom. The author treated 68 cases of epigastric pain with acupuncture, now it is reported as follows.     

Influence of Acupuncture on Cerebral Vasomotoricity of Ischemic Stroke

目的探讨针刺足三里和悬钟穴对缺血性中风脑血管舒缩反应能力的影响及其可能机制.方法将合适病例随机分为针刺组与对照组,每组80例,两组均予以脑卒中常规治疗,针刺组在此基础上结合针刺足三里和悬钟穴.在治疗前后用经颅多普勒检测大脑中动脉(MCA)血流速度的变化情况,以评价患者脑血管的舒缩反应能力.结果针刺组脑血管舒缩反应能力与治疗前相比显著加强,与对照组相比有显著性差异(P＜0.05).结论针刺足三里和悬钟对缺血性中风患者的脑血管舒缩反应能力有明显改善作用,这可能是针刺疗法治疗缺血性中风的起效机制之一.     

Safety of Individual Medication of Ma Qian Zi （Semen Strychni） Based upon Assessment of Therapeutic Effects of Guo＇s Therapy Against Moderate Fluorosis of Bone

Objective: To assess the safety of individual medication of Guo's Ma Qian Decoction on the basis of effective treatment of fluorosis of bone with Guo's therapy.Methods: One hundred and fourteen cases of moderate fluorosis of bone were randomly divided into a treatment group (n=60) and a control group (n=54) between December 2007 and August 2009 by using the block randomized method and a central random system.At the same time of basic treatment,the patients in the treatment group were orally administrated with Guo's Ma Qian Decoction.The initial dose of Ma Qian Zi (Semen Strychni) was 0.4 g and increased by 0.05 g every two days,with the doses of other drugs unchanged,until the patient had “nux vomica response”.For the patients with no “nux vomica response”,the dosage was continued to increase and the maximum dosage was not more than 1.2 g/day.The control group was treated with decoction placebo.The changes of strychnine and brucine contents before and after processing and after decoction of Ma Qian Zi (Semen Strychni)were determined with reversed-phase high-performance liquid chromatography,which were controlled within ranges stipulated in the Pharmacopeia; Adverse events were analyzed; Blood strychnine and brucine contents in 10 cases who had taken the drugs were determined.Results: 1) Strychnine (2.125％) and brucine (1.425％) contents before processing of Ma Qian Zi and 1.88％ and 1.31％ after processing all conformed with the standards of strychnine (1.2-2.2％) and brucine (no less than 0.8％) stipulated in the Pharmacopeia.When the maximum dosage of Ma Qian Zi was 1.2 g/day,strychnine in the decoction was 11.17 mg and brucine was 7.44 mg,which all conformed with the maximum limited amount (strychnine 13.32 and brucine no less than 4.8 mg) stipulated in the Pharmacopeia.2) Eight cases had “nux vomica response” in the treatment group and one case in the control group,with a significant difference between the two groups (P＜0.05).3) Altogether 18 cases had adverse events,with an incidence rate of 15.38％ (8 cases) in the treatment group and 18.52％ (10 cases) in the control group,with no difference between the two groups (P＞0.05);Among them,10 cases (8.77％) with the adverse event were not related with therapeutic drugs,with an incidence rate of 6.67％ (4 cases) in the treatment and 11.11％ (6 cases) in the control group,with no significant difference between the two groups (P＞0.05).Seven cases had suspicious relative adverse events,the risk in the treatment group was 0.658 times of the control group,with no significant difference (P＞0.05),and one case had the toxic reaction of nux-vomica seed.4) Strychnine and brucine were unable to be detected in the blood in all points of time in the 10 cases who had taken the drugs,indicating that plasma strychnine and brucine contents were lower than the minimum detectable amount (10 ng),and accumulation of strychnine and brucine were not found in blood of the patient during and after administration for 8 weeks.Conclusion: The individual medication ofMa Qian Zi (Semen Strychni) in the Guo's therapy has a better safety.     

Experience of Treatment of Depression by Acupuncture plus Medicine

Depression is a type of psychosomatic disorder that presents with persistent depressed emotion （or mood）, poor concentration and psychomotor inhibition. It is more than a psychological problem. However, with pathological changes of biology, depression can be either a major symptom of one condition or accompanying symptom of other conditions.     

Examples of Application of Ligou （LR 5）

To study the application and mechanism of Ligou(LR 5)in the treatment of urinary and reproductive system diseases from its location,meridian,application alone and combined application in clinic based on syndrome differentiation,and to guide its application in clinic.     

Clinical Study on Treatment of Obesity of Gastrointestinal Excessive Heat by Puncturing Acupoints of Stomach Meridian

目的讨胃经穴在胃肠实热型肥胖症治疗中的作用.方法51例胃肠实热型肥胖症患者随机分为两组,治疗组以胃经取穴为主,对照组采用俞募配穴法取穴.隔日治疗1次,1个月为1个疗程,共治疗3个疗程.观察患者的症状、体征的变化,测量治疗前后的肥胖指标及血瘦素、血脂、血糖、ACHE等实验室指标.结果与对照组相比,胃经组可显著减少各项肥胖指标(P＜0.01);改善肥胖患者的高瘦素血症(P＜0.05)和脂代谢紊乱(P＜0.05);逆转肥胖患者的副交感神经功能亢进的失衡状态(P＜0.05).结论经穴位可以作用于肥胖发生和发展的多个环节,是治疗胃肠实热型肥胖症的关键而有效的选穴.     

佐剂性关节炎大鼠血清IL-2、TNFα、Cort的变化及电针干预作用的研究

目的:观察并探讨电针抗炎免疫调节作用与血清白细胞介素-2(IL-2)、肿瘤坏死因子α(TNFα)、皮质醇(Cort)含量的相互关系,比较"大椎"穴、"命门"穴、尾部中点的作用差异。方法:以佐剂性关节炎大鼠(AA)作为研究对象,随机分为5组:正常组、模型组、电针"大椎"组、电针"命门"组、电针尾部中点组。观察电针对关节炎症局部及血清IL-2、TNFα、Cort含量的影响,并比较不同穴位间的作用差异。结果:"大椎"组、"命门"组经治疗IL-2含量与模型组比较极明显升高(P0.01),"大椎"组、"命门"组Cort含量,"大椎"组TNFα含量与模型组比较降低(P0.05),"大椎"组、"命门"组血清Cort含量与尾部中点组比较降低(P0.05)。结论:电针可能是通过血清IL-2、TNFα、Cort含量等因素的相互调节,起到抗炎免疫调节作用的。抗炎作用为"大椎""命门"尾部中点。     

电针干预对多囊卵巢综合征患者卵子质量及肿瘤坏死因子-α的影响

目的：观察电针对多囊卵巢综合征（PCOS）患者卵子质量的影响,并探讨其作用机制。方法：将接受体外受精-胚胎移植（IVF-ET）的PCOS患者200例按照随机数字表法分为电针组（102例）、对照组（98例）,两组均给予达英-35及促性腺激素释放激素激动剂超促排卵,电针组在超促排卵过程中加肾俞、气海、足三里、三阴交、内关、子宫等穴位电针干预,比较两组患者卵子质量、最终的妊娠结局及肿瘤坏死因子-α（TNF-α）水平。结果：（1）电针干预可显著提高PCOS患者的优胚率（P〈0.05）,并可将临床妊娠率提高8.36%;（2）电针干预可明显降低卵泡液TNF-α水平（13.61±15.46 vs 34.09±93.53,P〈0.05）;（3）妊娠患者血清、卵泡液TNF-α水平均低于非妊娠患者[妊娠：（53.91±63.32）pg/mL,（14.93±25.37）pg/mL,非妊娠：（76.82±82.96）pg/mL,（25.04±35.79）pg/mL]差异有统计学意义（均P〈0.05）。结论：电针能够改善PCOS患者卵子质量,提高IVF-ET的临床妊娠率,其机制可能与TNF-α水平有关。     

幽门螺旋杆菌感染与肿瘤坏死因子-α和白细胞介素-6的关系

目的：分析幽门螺旋杆菌（HP）感染时血清及胃黏膜组织肿瘤坏死因子（TNF）-α、白细胞介素（IL-6）的水平,探讨它们之间的关系。方法：采用放射免疫法和酶联免疫吸附实验夹心法分别检测了HP阳性患者在治疗前后,血清和胃黏膜组织中的TNF-α、IL-6的水平。结果：幽门螺旋杆菌感染程度越严重者,胃黏膜组织中TNF-α、IL-6水平越高,各组间差异均有显著意义（P〈0.05）。HP根除后血清、胃黏膜组织中TNF-α、IL-6水平明显下降,与根除前相比有显著性差异（P〈0.05）。结论：TNF-α、IL-6与HP感染密切相关,且参与了胃黏膜损伤的病理过程。     

温和灸腹部募穴对实验性大鼠肠道菌群失调的影响

目的通过温和灸关元、天枢两个腹部募穴,观察其对肠道菌群失调的影响。方法清洁级Wistar大鼠50只,雌雄各半,体重（200±20）g,采用随机对照方法分为正常组（A）、模型组（B）、药物组（C）、关元组（D）、天枢组（E）,每组10只。用大量盐酸林可霉素灌胃造模,造模成功后,分别进行药物治疗和温和灸法治疗。1个疗程后,各组大鼠均被迫采取新鲜粪便0.1 g,应用双歧杆菌（BS）、乳酸杆菌（LBS）、肠杆菌（EMB）、肠球菌（EC）选择性培养基进行细菌培养,生化鉴定管和比浊法检测不同菌落生长情况和各组菌落数量。结果温和灸关元穴使BS、LBS数量有所增加,温和灸天枢穴使EMB、EC数量有所增加。结论温和灸不同部位的募穴可以选择性调整肠道优势益生菌群,从而治疗肠道菌群失调症。     

加昧小陷胸汤对实验性心肌缺血再灌注的作用

目的：研究小陷胸汤加味方对心肌缺血再灌注心肌的作用。方法：将32只健康家兔随机分为4组,假手术组（C组）,小陷胸汤加味方高剂量组（D组）,每组8只。A组不予缺血再灌注处理,C、D两组提前5天连续灌胃中药,A、B组各灌以等量0．9％NaCl溶液。B、C、D三组建立心肌缺血再灌注模型。各组分别于再灌注120min检测血清磷酸肌酸激酶（CK）,肿瘤坏死因子-α（T．F-α）的含量。结果：C、D两组CK、T。F-α均低于B组（P〈0．05）,C、D两组间CK、T．F-α无差异（P〉0．05）。结论：小陷胸汤加味方可降低兔心肌缺血再灌注CK、T．F-α含量,对心肌缺血再灌注损伤具有保护作用。     

益肾保真方对慢性肾衰竭大鼠肾纤维化干预作用的实验研究

目的探讨益肾保真方对慢性肾衰竭（CRF）大鼠肾纤维化的干预作用及其机制。方法采用两步分肾切除法复制5／6肾切除大鼠慢性。肾衰竭模型,随机分为假手术组、模型组、益。肾保真方高剂量组、益肾保真方低剂量组及尿毒清组。各组均干预6周,观察大鼠的血肌酐、尿素氮、尿蛋白定量及肾组织病理变化情况,检测肾小球硬化指数、肾组织Ⅳ型胶原含量及结缔组织生长因子（CTGF）、α-平滑肌肌动蛋白（α-SMA）的表达。结果与模型组比较,益肾保真方组尿蛋白、血肌酐、尿素氮、肾组织IV型胶原含量均显著降低,与尿毒清组比较差异无统计学意义（P〉0．05）;与模型组比较,益肾保真方组大鼠肾小球硬化指数降低,肾组织CTGF、α-SMA表达下调,与尿毒清组比较差异有统计学意义（P〈0．05）。结论益肾保真方能延缓肾衰竭进展,干预肾纤维化进程,其机制可能与下调肾组织CTGF、α—SMA的表达,抑制肾脏固有细胞的转分化,从而减少细胞外基质成分的合成和积聚有关。     

电针足三里、中脘对急性胃黏膜损伤大鼠NO、GAS、EGF的影响

目的:观察足三里与中脘配伍电针对表皮生长因子(EGF)、一氧化氮(N0)、胃泌素(GAS)的影响,来探讨单穴或多穴配伍治疗对胃黏膜损伤修复作用的差异。方法:50只大鼠随机分为5组,即足三里组、中脘组、足三里配中脘组、模型组、空白对照组。每组10只。用无水乙醇按0.6 ml/100 g灌胃,造成胃黏膜损伤模型。各组检测胃黏膜EGF、血清和胃黏膜N0、GAS含量。结果:各电针组EGF、NO含量明显高于模型组(P0.05),GAS含量明显低于模型组(P0.05);足三里组、中脘组之间没有显著性差异(P0.05),而足三里配中脘组与这两组相比较均有显著性差异(P0.05)。结论:足三里、中脘两穴配伍对胃黏膜损伤修复有协同增效作用。     

凝血四项在临床孕妇中检测的意义

目的:探讨临产孕妇凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(Fbg)和D-二聚体(DD)检测的临床意义。方法:对151例临产孕妇(实验组)与144例正常非妊娠妇女(对照组)的凝血功能进行比较分析。结果:临产孕妇组PT、APTT、Fbg和DD相应与对照组的PT、APTT、Fbg和DD比较差异有统计学意义(P<0.01)。结论:在产前及分娩过程中,及时监测各项凝血指标对预测和治疗产妇异常出血有重要意义。     

小针刀疗法对膝关节骨性关节炎患者肿瘤坏死因子-α及一氧化氮的影响

目的：观察小针刀疗法对膝关节骨性关节炎（ KOA）患者关节液肿瘤坏死因子-α（ TNF-α）及一氧化氮（ NO）表达的影响,探讨小针刀的作用机制。方法将120例KOA患者随机分为2组。治疗组60例采用小针刀治疗,对照组60例采用普通针刺治疗。6周后检测比较2组患者治疗前后关节液中TNF-α及NO的水平,同时评定临床疗效。结果2组治疗后关节液中TNF-α及NO水平均较本组治疗前降低（ P＜0．05,P＜0．01）,且治疗组下降更为明显（P＜0．01）。2组治疗后症状均有不同程度的改善,治疗组总有效率（78．3％）优于对照组（71．7％）（P＜0．05）。结论小针刀疗法治疗KOA比常规毫针刺法更有效。     

中药对兔退变椎间盘内TNF-α的影响

目的：探讨中药综合治疗对兔腰椎间盘退变中炎性因子TNF-α的影响,观察中医药综合治疗对腰椎间盘退变的作用机制。方法：选用健康新西兰大白兔90只,雌雄各半,平均6月龄,体重平均约2.5 kg。通过纤维环穿刺的方法,建立椎间盘退变模型。90只新西兰兔随机分成5组：正常组、模型组、中药组、贴敷组、中药＋贴敷组。正常对照组18只不造模,余72只行造模手术,各组分别于治疗后2、4、6周各处死6只,取髓核组织检测TNF-α含量。结果：模型对照组中TNF-α的含量与正常组相比较明显升高;所有治疗组2周后椎间盘组织中TNF-α的含量与模型组相比较均明显降低,中药＋贴敷组2周后TNF-α的含量与单独内服中药及贴敷比较有明显降低。结论：退变后的椎间盘中释放的炎性因子TNF-α含量会增加;所有干预组在2周后均能降低退变椎间盘中炎性因子TNF-α的含量;综合运用2种干预（中药＋贴敷）2周后治疗效果优于单独使用中药或贴敷治疗。