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Background
Invasive fungal infections are an emerging health problem worldwide. They are responsible for a significant rate of morbidity and mortality. Infections caused by Candida albicans involve proliferation of biofilms on biotic or abiotic surface. These adherent communities exhibit characteristics distinct from planktonic cells such as the ability to tolerate high concentrations of antifungal.Objective
The object of our study was focused on the determination of the susceptibility to amphotericin B, caspofungin, voriconazole and two antifungal combinations (amphotericin B/caspofungin and amphotericin B/voriconazole) of both planktonic and sessile cells of C. albicans, which were isolated from catheters.Material and methods
The susceptibility of C. albicans to antifungals was determined using the broth microdilution method according to Clinical Laboratory Standards Institute CLSI (2008). A Checkerboard assay was employed to evaluate the efficacy of drugs combinations. Biofilm susceptibility was determined using a metabolic [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (XTT) reduction assay.Results
The minimal inhibitory concentrations of individual antifungal drugs determined against C. albicans biofilms (SMICs) were significantly higher (P < 0.05) than planktonic ones (MICs). They went from 2 to 64 μg/mL for amphotericin B, from 1 to 64 μg/mL for caspofungin and from 2 to 128 μg/mL for voriconazole. The combination of amphotericin B to caspofungin or to voriconazole decreased significantly the MIC values for planctonic (P < 0.0001) and sessile cells (P = 0.0016). Based on Fractional Inhibitory Concentration Index (FICI), no antagonistic interaction was observed.Conclusion
The obtained results showed that the combination of amphotericin B with either caspofungin or voriconazole can be used as a new strategy for management of systemic mycoses associated to medical devices. 相似文献2.
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A.R. Khosravi A. Sharifzadeh D. Nikaein Z. Almaie H. Gandomi Nasrabadi 《Journal de Mycologie Médicale》2018,28(2):355-360
Systemic candidiasis has become an emerging fungal infection in recent years. Anti-Candida resistance to conventional antifungal agents has subsequently increased. This study reported the chemical composition, antioxidant and anti-Candida activity of Origanum majorana, Artemisia dracunculus, Cymbopogon citrate, Cinnamomum verum and Caryophyllus aromaticus essential oils. Different Candida species, from urine tracts of hospitalized patients, were included to be challenged with understudied essential oils. Chemical compositions were determined using gas chromatography/mass spectroscopy (GC/MS) analysis and antioxidant activity was measured using DDPH assay. MIC of these essential oils was evaluated using broth micro-dilution test. Caryophyllus aromaticus had the highest antioxidant activity while the lowest antioxidant activity was for Artemisia dracunculus. MICs of Cinnamomum verum, Caryophillium aromaticus, Artemisia dracunculus, Origanum vulgare and Cymbopogon citratus essential oils ranged from 125 to 175 μg/mL (mean value: 147.7 ± 25.5 μg/mL), 700 to 1000 μg/mL (mean value: 740.9 ± 105.4 μg/mL), 1000 to 2000 μg/mL (mean value: 1454.5 ± 509.6 μg/mL), 173 to 350 μg/mL (mean value: 208 ± 55.8 μg/mL) and 125 to 175 μg/mL (mean value: 156.8 ± 24.6 μg/mL) for different Candida species, respectively. In general, natural compounds are suitable to be used as anti-Candida and antioxidant agents. However in this stage, these compounds could be applied as supplementary agents along with conventional antifungal drugs. 相似文献
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《Journal de Mycologie Médicale》2015,25(4):e118-e127