Isolation and characterization of eighteen polymorphic microsatellite loci for the topical seagrass, Syringodium isoetifolium

Eighteen polymorphic microsatellite loci were developed for the tropical seagrass, Syringodium isoetifolium. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Pacific Biosciences SMRT technology. The 18 loci were characterized in 24 individuals from the Indonesian archipelago. Observed levels of heterozygosity ranged from 0.14 to 0.91 with a mean of 3.7 alleles per locus. Four pairs of loci showed evidence of significant linkage disequilibrium and one locus deviated significantly from Hardy–Weinberg expectations.     

Isolation and characterization of twenty-four polymorphic microsatellite loci in Oxygymnocypris stewartii

Twenty-four polymorphic microsatellite loci were isolated from Oxygymnocypris stewartii and were characterized in 42 individuals collected from the Yarlung Tsangpo River in Tibet, China. The number of alleles per locus ranged from 4 to 24 with an average of 12.542. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.731 to 0.932 and from 1.342 to 2.887, respectively. These polymorphic loci will be available for the evaluation of genetic diversity and population genetic structure of O. stewartii.     

Development of microsatellite markers for Thalassia hemprichii (Hydrocharitaceae), a widely distributed seagrass in the Indo-Pacific

Nine microsatellite markers of a widely-distributed seagrass, Thalassia hemprichii, were developed to investigate genetic diversity and genetic connectivity. The number of alleles detected per locus ranged from 3 to 26, and the expected and observed heterozygosities ranged from 0.00 to 1.00 and 0.00 to 0.87, respectively. The markers described here are sufficiently polymorphic and informative to investigate the genetic diversity, genetic connectivity and genetic structure of T. hemprichii.     

Isolation and characterization of twenty-three polymorphic microsatellite loci for Linckia laevigata

Twenty-three polymorphic microsatellite loci were developed for the tropical sea star, Linckia laevigata. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Roche 454 technology. The 23 loci were characterized in 21 individuals from Pulau Derawan, Indonesia. Observed levels of heterozygosity ranged from 0.20 to 1.00 with a mean of 8.0 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and 4 loci significantly deviated from Hardy–Weinberg expectations.     

Isolation and characterization of fourteen polymorphic microsatellite loci for Odontobutis sinensis

Odontobutis sinensis is an indigenous freshwater fish in China. The wild population size of this species has declined sharply in inland waters in recent years. For the purpose of the conservation of natural resources of O. sinensis, 14 polymorphic microsatellite loci were isolated and characterized in 30 individuals collected from Liangzi Lake in Hubei Province. The number of alleles per locus ranged from 2 to 14 with an average of 5.07. The expected and observed heterozygosities ranged from 0.291 to 0.887 and from 0.067 to 0.700, respectively. These newly isolated loci are currently being used for population genetic diversity analysis and will be valuable for the conservation of O. sinensis.     

Characterization of twenty-four polymorphic microsatellite loci of Rasbora borapetensis

Twenty-four microsatellite loci were isolated and characterized from the genome of Rasbora borapetensis. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic with allele numbers ranging from 2 to 27, observed heterozygosity from 0.031 to 1.000 and expected heterozygosity from 0.031 to 0.965. All loci conformed to the Hardy–Weinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci will enhance future investigations on the genetic differentiation and structure of populations of Rasbora borapetensis.     

Isolation and characterization of polymorphic microsatellite loci from Silurus Lanzhouensis

Silurus Lanzhouensis is an endangered freshwater fish endemic to Ningxia, Gansu and Shaanxi province of China. Here we describe a first set of 12 polymorphic Silurus Lanzhouensis microsatellite loci. The observed numbers of alleles ranged from 2 to 7. The expected and observed heterozygosities ranged from 0.3279 to 0.8438 and from 0.4021 to 0.8410, respectively. These microsatellite markers are useful for population and conservation genetic studies in S. Lanzhouensis.     

Isolation and characterization of twenty-three polymorphic microsatellite loci in Schizothorax macropogon

Twenty-three polymorphic microsatellite loci were isolated from Schizothorax macropogon and were characterized in 42 individuals collected from the Yarlung Tsangpo River in Tibet, China. The number of alleles per locus ranged from 5 to 32 with an average of 20.17. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.709 to 0.951 and from 1.421 to 3.183, respectively. These microsatellite loci will be valuable for the evaluation of genetic diversity and population genetic structure of S. macropogon.     

Isolation and characterization of seventeen polymorphic microsatellite loci for the tropical sea star, Linckia multifora

Seventeen polymorphic microsatellite loci were developed for the tropical sea star, Linckia multifora. Microsatellite enriched genomic libraries were constructed and sequenced using Roche 454 technology. The 17 loci were characterized in 24 individuals from the Kei islands, Indonesia. Observed levels of heterozygosity ranged from 0.29 to 1.00 with a mean of 6.0 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and four loci significantly deviated from Hardy–Weinberg expectations.     

Isolation and characterization of polymorphic microsatellite loci for Imperata cylindrica, an invasive perennial grass

Cogongrass (Imperata cylindrica) is an invasive perennial grass widely distributed in the world, and two ecotypes (C-type and E-type) are found in Japan. We isolated and characterized eight microsatellite loci from the two ecotypes. The number of alleles ranged from 4 to 12 in C-type, 1 to 7 in E-type and 4 to 12 in the hybrid between them. The range of expected heterozygosities was 0.561–0.910 in C-type, 0.000–0.823 in E-type and 0.541–0.918 in the hybrid. These markers will be useful for investigating expansion process, natural hybridization between two ecotypes and reproductive strategy of this weed.     

Isolation and characterization of 24 polymorphic microsatellite loci for the veined rapa whelk,Rapana venosa

Twenty-four polymorphic microsatellite loci were isolated and characterized for Rapana venosa. The number of alleles per locus ranged from 4 to 32, and the observed and expected heterozygosity ranged from 0.3226 to 1.0000 and from 0.5754 to 0.9727, respectively. These markers should prove a useful tool to investigate population genetic structure and the colonization process of R. venosa.     

Isolation and characterization of twenty-six, polymorphic microsatellite loci for the tropical sea urchin, Colobocentrotus atratus

Twenty-six, polymorphic microsatellite loci were developed for the tropical sea urchin Colobocentrotus atratus. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Roche 454 technology. The 26 loci were characterized in 21 individuals from Pulau Asu, Nias, Indonesia. Observed levels of heterozygosity ranged from 0.52 to 1.00 with a mean of 11.9 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and 3 loci significantly deviated from Hardy–Weinberg expectations.     

Isolation and characterization of 11 polymorphic microsatellite loci in the Japanese halfbeak,Hyporhamphus sajori

Conservation Genetics Resources - Eleven polymorphic microsatellite loci were isolated and characterized in Hyporhamphus sajori. The number of alleles per locus ranged from 2 to 23, and the...     

Isolation and characterization of twelve polymorphic tetranucleotide microsatellite loci from Yerana yei

Twelve highly polymorphic microsatellite markers were isolated and characterized for the frog Yerana yei. Loci were isolated from a genomic library from Y. yei enriched for (ATAG)_n repetitive elements. We designed primers that reliably amplified twelve polymorphic loci and tested them on 32 individuals from two populations. The number of alleles per locus ranged from 3–20, the observed and expected heterozygosity ranged within 0.500–1.000 and 0.752–0.964 respectively, and the polymorphic information content (PIC) value ranged from 0.683–0.930. These microsatellite markers could be valuable in future research concerning the conservation genetics and molecular ecology of this species.     

Isolation and characterization of nineteen novel polymorphic microsatellite loci for the northern snakehead Channa argus

Channa argus is one of the most important economic fish species in China. However, there is limited genetic information on its population structure and genetic diversity. Nineteen novel polymorphic microsatellite loci were isolated and characterized in 42 individuals from one natural population collected from the Poyang Lake in Jiangxi Province. The number of alleles per locus ranged from 5 to 18 with an average of 11.16. The observed and expected heterozygosities ranged from 0.214 to 1.000 and from 0.630 to 0.923, respectively. The average polymorphic information content value was 0.814. Among these polymorphic microsatellites, eleven loci conformed to Hardy–Weinberg equilibrium. These microsatellite loci are currently being used to assess the genetic diversity and population structure of C. argus and will contribute to the effective conservation and rational utilization of genetic resources of this species.     

Isolation and characterization of polymorphic microsatellite loci primers for Cupressus funebris (Cupressaceae)

Cupressus funebris Endl. (Cupressaceae) is an endemic conifer species in central and eastern China. In order to investigate the genetic diversity within and between populations and design the effective conservation strategies, we aimed to develop microsatellite markers for this species in the present study. We isolated and characterized 12 novel polymorphic microsatellite loci for this species through the combined biotin capture method. Polymorphism of each locus was further assessed through 62 individuals from three geographically distant populations. The number of alleles per locus ranged from 5 to 12. The observed and expected heterozygosity per locus ranged from 0.115 to 0.396, and 0.204 to 0.542, respectively. One locus (Cf07) showed significant deviation from Hardy–Weinberg equilibrium and no Linkage disequilibrium was detected after Bonferroni corrections. These microsatellite markers will be useful for population genetics studies of this conifer species.     

Isolation and characterization of fifteen polymorphic tetranucleotide microsatellite loci in Schizopygopsis younghusbandi Regan

Schizopygopsis younghusbandi Regan is an important endemic fish in Tibet, China. Its wild population has been rapidly declining for the past few decades due to overfishing, dam construction and invasion of exotic fishes. To conserve natural resources and develop breeding stocks of S. younghusbandi, fifteen polymorphic tetranucleotide microsatellite loci were isolated and characterized in 45 individuals collected from the Yarlung Tsangpo River in Tibet. The number of alleles per locus ranged from 6 to 32 with an average of 17.87. The expected heterozygosity and Shannon–Wiener diversity index ranged from 0.454 to 0.951 and from 0.931 to 3.201, respectively. These microsatellite loci will be useful for future population genetic analysis and resource conservation of S. younghusbandi.     

Isolation and characterization of twenty-one polymorphic microsatellite loci for Polycarpa aurata using third generation sequencing

Twenty-one microsatellite loci were developed for the tropical ascidian, Polycarpa aurata. Microsatellite enriched genomic libraries were constructed and sequenced using Pacific Biosciences single molecule real time sequencing technology. The 21 loci were characterized in 20 individuals from Alor, Indonesia. Observed levels of heterozygosity ranged from 0.30 to 1.00 with a mean of 8.2 alleles per locus. Loci PA21 and PA45 showed evidence of significant linkage disequilibrium and three loci significantly deviated from Hardy–Weinberg expectations.     

Isolation and characterization of polymorphic microsatellite loci from the golden mouse (Ochrotomys nuttalli)

Thirteen polymorphic microsatellite loci were developed for golden mouse using an enrichment protocol. The markers were tested on 37 golden mouse specimens collected from southern Illinois. Numbers of alleles ranged from 3 to 11 with observed heterozygosities ranging from 0.29 to 0.92. Three of the loci exhibit nominally significant excesses of homozygosity, but none were significant following a correction for multiple tests. None of the loci appear to be linked. Although golden mouse is not considered at risk in the core of its range, peripheral populations in several states are considered threatened or otherwise at risk due to low population densities. Because of the evolutionary distinctiveness of Ochrotomys, species-specific primers are needed to identify patterns of gene flow among core and peripheral populations.     

Isolation and characterization of polymorphic microsatellite loci from the invasive plant Lantana camara L.

Lantana camara is a highly invasive plant that has established itself in at least 60 different countries across the world. Here, we report development of ten microsatellite markers for this species. These microsatellite loci have 2?C15 alleles per locus; with observed and expected heterozygosity of 0.022?C0.833 and 0.336?C0.848, respectively. These markers will be useful in addressing a variety of questions about Lantana camara, including those concerning breeding system, pollination and dispersal, genetic variation and population structure.