外周血GPC-3 mRNA在大鼠肝癌微转移中的意义

目的在大鼠肝细胞癌模型中，通过检测外周血GPC-3 mRNA，探讨其与大鼠肝癌微转移的关系，为临床肝癌的早期检测奠定实验基础。方法采用二乙基亚硝胺建立大鼠肝癌模型，提取外周血总RNA，经逆转录后，用巢式PCR检测GPC-3 mRNA和AFP mRNA，比较二者在外周血中的阳性率与肝癌微转移的关系。结果75例成功构建的肝癌大鼠中，其中25例单个结节肝癌中GPC-3 mRNA阳性率为32％，AFP mRNA阳性率为28％；20例2～5个结节肝癌中GPC-3 mRNA阳性率为45％，AFP mRNA阳性率为35％；18例大于5个结节肝癌中GPC-3 mRNA阳性率为72．2％，AFP mRNA阳性率为61．1％；12例其它器官有明显转移灶的肝癌中GPC-3 mRNA阳性率为83．3％，AFP mRNA阳性率为75％；18例正常大鼠中GPC-3未检测到，而AFP mRNA阳性率为11．1％。结论随着肿瘤发生转移的可能性增大，GPC-3 mRNA的阳性率明显增高，表明外周血GPC-3 mRNA的阳性率与肝癌微转移的发生有明显的相关性，且高于AFP mRNA的阳性率。有望成为肝癌微转移新的诊断指标。     

应用巢式RT-PCR联合检测原发性肝癌病人外周血AFPmRNA和MAGE-1mRNA的表达

目的 寻找一种敏感的方法以早期发现原发性肝癌的复发与转移。方法 应用逆转录聚合酶链反应分别在术前、术后1周和术后2个月检测37例行根治性切除的原发性肝癌病人外周血中MAGE-1 mRNA和AFPmRNA的表达，并随访所有病人1年。结果 外周血中MAGE-1 mRNA和AFPmRNA的阳性率和肿瘤的病理分期相关；肿瘤病期越晚，外周血中微转移灶的检出率越高。1年的随访中，有9例病人出现复发，MAGE-1和(或)AFPmRNA的阳性率为77．8％(7／9)，其中6例外周血中MAGE-1mRNA和(或)AFPmRNA持续阳性，2例由阴性转为阳性。另有10例病人MAGE-1mRNA和(或)AFPmRNA由术前阳性术后转为阴性，随访1年中无复发。结论 联合检测外周血MAGE-1和AFPmRNA的表达是早期发现肝癌术后复发、评估预后一项敏感可信的指标。     

肝癌患者不同部位血甲胎蛋白mRNA的检测及其临床意义

目的 检测肝癌患者不同部位（门静脉、肝静脉及外周静脉）血甲胎蛋白（AFP）mRNA的表达,了解肝细胞肝癌血行播散的规律。方法 采用巢式逆转录－聚合酶链式反应（RT－PCR）检测技术,测定20例肝癌患者门静脉、肝静脉及外周血AFPmRNA的表达。结果 全组病例门静脉、肝静脉血AFPmRNA阳性率（65％）高于其外周血AFPmRNA阳性率（40％－45％）,但差异无显著性（P＞0．05）。肿瘤≤5cm患者的肝静脉血AFPmRNA阳性率（70％）明显高于外周血（20％,P＜0．05）,门静脉血AFPmRNA阳性率（60％）也高于外周血（30％）,但差异无显著性（P＞0．05）。肿瘤＞5cm患者的门静脉、肝静脉血AFPmRNA阳性率接近于外周血AFPmRNA的阳性率。结论 肿瘤＞5cm的中晚期肝癌患者,其外周血中AFPmRNA的表达能较好地反映脱落癌细胞的血行播散情况;肿瘤≤5cm的患者虽然其外周血AFPmRNA阳性检出率较低,但肝静脉血的AFPmRNA明显高于外周血,提示肝内已有肿瘤播散,此时直接检测肝内门静脉或肝静脉血的AFPmRNA表达能更准确地反映癌细胞血行播散情况。     

肝癌组织、外周血细胞磷脂酰肌醇蛋白聚糖3 mRNA表达的意义

目的探讨磷脂酰肌醇蛋白聚糖3(GPC3)mRNA表达对肝细胞肝癌(HCC)及其血液微转移的诊断价值。方法参照甲胎蛋白(AFP)mRNA,半定量与巢式逆转录聚合酶链反应(RT-PCR)检测41例HCC、41例癌旁组织、52例非瘤肝组织(41例远离癌灶组织与11例正常肝组织)及67例外周血的GPC3表达。结果半定量PCR显示组织与外周血细胞全部存在GPC3 mRNA表达,HCC、癌旁、非瘤肝组织的表达量分别为78.9±35.5、30.6±21.6、23.8±15.5;而AFP mRNA的表达量依次为61.2±32.6、31.5±23.6、21.2±15.9。各基因在HCC与其他组织中的表达量差异具有统计学意义(P<0.01),以各基因在非瘤肝的相对表达量上限(x-+1.96s)为界,HCC中GPC3 mRNA和AFP mRNA的高表达分别占80.5%与63.4%;至少一个基因高表达HCC达总数的92.7%,与AFPmRNA单独检测差异有统计学意义(P<0.01)。临床病理特征分析发现AFP mRNA与HCC分级及血清AFP浓度有关,GPC3 mRNA表达与HCC分级及侵袭性有关。67例外周血GPC3 mRNA的表达量为15.9±9.0,各类组织中GPC3 mRNA表达均强于相应的外周血表达(P<0.01)。外周血GPC3mRNA表达量在HCC组、非HCC组分别为16.1±8.3、15.6±10.2,组间差异无统计学意义;在HCC转移组、HCC无转移组的表达量各为16.0±9.0、16.3±7.7,组间差异无统计学意义。巢式PCR显示,AFP mRNA表达比率在HCC组与非HCC组各为56.1%、23.1%,组间差异有统计学意义(P=0.011);HCC转移组与无明显转移组各为80.9%、30.0%,组间差异有统计学意义(P=0.002)。结论GPC3 mRNA是潜在的HCC组织诊断指标,与AFP mRNA有明显的互补作用,两者联合检测可提高HCC的阳性检出率;外周血存在GPC3 mRNA的广泛表达,但其与HCC的转移复发无关,不能用于HCC微转移的检测。     

外周血甲胎蛋白mRNA和黑色素瘤抗原-1 mRNA联合表达与肝细胞癌术后复发转移的关系

目的 探讨外周血甲胎蛋白（AFP）mRNA和黑色素瘤抗原-1（MAGE-1）mRNA联合表达在早期发现肝细胞癌患者术后复发或转移的意义。方法 应用巢式逆转录聚合酶链反应技术分别在术前、术后1周和2个月检测142例行根治性切除的肝细胞癌患者外周血中AFPmRNA和MAGE-1mRNA的表达，随访时间从术后至22个月，中位随访时间为15．5个月。结果 外周血中MAGE-1mRNA和／或AFPmRNA的阳性率和肿瘤的病理分期呈正相关：肿瘤病期越晚，外周血中肝癌细胞的检出率越高（χ^2=17．873，P〈0．01）。AFPmRNA和Mage-1mRNA阳性率均与癌结节数目、门静脉癌栓、肝内、外转移等临床参数显著相关（χ^2=11．510，χ^2=15、149，χ^2=11．653，χ^2=6．805，均P〈0.01），而与肝癌分化程度、肿瘤直径等无相关性（χ^2=1．513，χ^2=1．343，均P〉0．05）。术后55例患者出现复发或转移，其中38例患者外周血MAGE-1mRNA和／或AFPmRNA持续阳性，14例由术前阴性转为阳性。而62例由术前阳性转为术后阴性者，随访中未见复发或转移。术后外周血AFPmRNA和／或MAGE-1mRNA阳性组中，有88．1％（52／59）的患者出现复发或转移，而阴性组中复发或转移者仅占3．6％（3／83），两组比较，差异有统计学意义（χ^22=103．817，P=0．000）。结论 联合检测外周血MAGE-1和AFPmRNA的表达可能早期发现肝细胞癌术后复发或转移，可以作为评估预后的指标。     

肝癌射频消融治疗前后外周血微转移变化的研究

目的: 探讨射频消融(RFA)治疗是否促进原发性肝癌（PHC）患者的血源性转移。方法:应用RT-PCR检测32例PHC患者RFA治疗前后外周静脉血AFPmRNA的表达。结果:32例PHC患者RFA治疗前外周血AFPmRNA表达阳性18例(56.3%),阴性14例(43.7%);治疗后阳性12例(37.5%),阴性20例(62.5%)。RFA治疗前后PHC患者外周血AFPmRNA阳性率无显着性差异(P>0.05)。结论:RFA治疗肝癌不会促进肿瘤的血源性转移。     

联合检测肝细胞癌患者外周血甲胎蛋白和黑色素瘤抗原-1 mRNA的临床意义

目的　探讨甲胎蛋白 (α FP)mRNA和黑色素瘤抗原 1(MAGE 1)mRNA联合检测肝细胞癌患者外周血微转移的可行性。方法　采用巢式逆转录聚合酶链反应 (RT PCR)技术检测 6 5例原发性肝细胞癌、2 2例肝炎和肝硬化、12例继发性肝癌、12例肝血管瘤患者、2 0例健康献血员外周血α FPmRNA和MAGE 1mRNA水平。结果　肝细胞癌患者外周血中 ,α FPmRNA和MAGE 1mRNA的检出率为分别是 5 4 % (35 /6 5 )和 4 2 % (2 7/6 5 ) ,6 5 % (42 /6 5 )的患者至少一种标志物阳性。α FPmRNA和MAGE 1mRNA的检出率和肝癌结节数目、门静脉癌栓、肿瘤直径、TNM分期等临床参数密切相关 (χ2 值分别为 8 0 2 3与 8 4 4 0 ,9 6 73与 5 132 ,6 777与 7 0 99,11 0 37与 8 5 4 7,P <0 0 5 )。α FPmRNA在肝炎肝硬化患者外周血中的检出率为 14 % (3/2 2 ) ,继发性肝癌、肝血管瘤患者及健康献血员中均未检出。MAGE 1mRNA在继发性肝癌患者外周血中的检出率为 33% (4/12 ) ,肝炎肝硬化、肝血管瘤患者及健康献血员中均未检出。结论　以α FPmRNA和MAGE 1mRNA为指标 ,采用巢式RT PCR技术检测肝细胞癌患者外周血微转移可行 ,两者联合检测可能有助于提高肝细胞癌外周血微转移检测的敏感性和特异性     

GPC3 mRNA在甲胎蛋白阴性肝癌中的表达及其意义

目的 探讨GPC3基因在甲胎蛋白阴性肝癌中的变化及其临床意义。方法 应用印迹法（Northern)杂交检测48例肝癌组织及其相应的癌旁肝细胞内GPC3mRNA表达的变化,其中肝细胞癌41例、肝内胆管细胞癌4例、大肠癌肝转移2例和肝局灶性结节增生1例,并结合各组织标本的病理学特点进行分析。结果 41例甲胎蛋白阴性肝细胞癌中,有30例肝癌组织可测出GPC3mRNA的表达（73.17%),而癌旁组织中均不表达。直径＞5cm的大肝癌GPC3的表达率显著高于直径≤5cm的小肝癌,低分化肝癌GPC3 的表达率显著高于高分化的肝癌。GPC3的表达与患者的年龄、性别、包膜完整性、癌栓形成、肝内转移及乙肝病毒感染状况均无明显相关性。GPC3mRNA在非肝细胞肝癌组织内均不表达。结论 GPC3mRNA在AFP阴性肝癌中特异性表达,可作为原发性肝癌的一个新的基因标志。GPC3mRNA在肝癌的发生发展中有重要作用。     

外周血γ-谷氨酰转移酶mRNA-H亚型与甲胎蛋白对原发性肝癌诊断及肝外转移意义的比较

目的　探讨外周血γ 谷氨酰转移酶 (GGT)mRNA H亚型及甲胎蛋白 (AFP)的表达与原发性肝癌 (HCC)诊断及肝外转移的关系。方法　以逆转录 聚合酶链反应方法检测HCC及对照组外周血GGTmRNA H亚型 ;运用放射免疫测定法检测外周血AFP水平。结果　 65例肝癌中 ,GGTmRNA H亚型阳性率为 46.2 %。小肝癌AFP阳性率 (3 0 .0 % )明显低于大肝癌 (75 .6% ) ;其余各组AFP阳性率无差异 (P >0 .0 5 ) ;有肝内转移 (82 .1% )、门静脉癌栓 (86.4% )及远处转移组(90 .0 % )其外周血GGTmRNA H亚型阳性率显著高于无肝内转移 (18.9% )、门脉癌栓 (2 5 .6% )及远处转移组 (3 8.2 % ) (P <0 .0 5 )。血清AFP值的高低与肝癌患者外周血GGTmRNA H的阳性率两者差异无显著性 (P >0 .0 5 )。结论　检测HCC患者外周血细胞GGTmRNA H亚型表达对估计有无血行播散和远处转移有临床实用价值。对血清AFP值低或阴性患者进行周围血GGTmRNA H测定可以显著提高肝癌诊断的敏感性和特异性     

围手术期检测肝细胞癌病人外周血AFP mRNA的临床意义

目的　检测围手术期肝细胞癌病人外周血AFPmRNA ,通过随访评估其预后价值。方法　通过巢式RT PCR的方法 ,在围手术期检测了 4 5例肝细胞癌病人外周血中AFPmRNA的表达 ,并进行了 1～ 2 0个月不等的随访。结果　在 4 5例肝细胞癌病人外周血中 ,AFPmRNA的检出率分别是术前 5 1.1% ,术后 7d 2 4 .4 % ,术后 2 8d 2 2 .2 %。多因素统计分析提示 ,术后 2 8d外周血检出AFPmRNA是提示病人复发的独立预后因素。结论　采用巢式RT PCR的方法检测肝细胞癌病人术后 2 8d外周血AFPmRNA有助于预测肝癌转移复发     

杭州健康女性定量骨超声测定原发性骨质疏松

目的 评价杭州健康女性骨超声速度(SOS)值随增龄减少和骨质疏松患病率，建立杭州地区女性骨超声速度值参考数据库。方法 定量超声法测定1208例杭州地区健康女性桡骨远端(RAD)，第3指骨近节(PLX)，第V跖骨(MTR)和胫骨中段(TIB)的超声速度值。结果 RAD、PLX、MTR和TIBSOS峰值(Peak of SOS)均出现在40-45岁，TJB的SOS峰值出现在35—40岁，此后随年龄增长而下降。绝经后妇女在绝经后早期和晚期各有1个SOS快速减少期，前见于桡骨近端，平均年减少率为2．4％，后见于胫骨中段，平均年减少率为1．8％。各部位骨SOS累积减少率随年龄增长而增加，到85岁4部位累积减少为13％-18％。60岁以后骨质疏松性症(OP)检出率为45％-70％，OP检出率以桡骨远端最高，60-70岁平均为67％，第3指骨近端次之约50％，胫骨中段最低为36％；75岁以后分别为70％，65％和45％。结论 全身各部位骨超声速度值到达峰值的年龄不同，峰值也各有差异。绝经后妇女骨超声速度值随年龄增加减少较快，应予激素和补钙治疗，桡骨远端为本地区SOS检测和OP检出的敏感部位。     

Importance of echocardiography in prognosis of surgical outcomes in cholecystitis in elderly patients

The authors propose to use more often echocardiography (EchoCG) in examination of elderly (over 60 years) of age patients with cholecystitis that permits to increase surgical activity to 92.4%. Left ventricular ejection fraction is the most informative. When this fraction is lower than 45% surgery must be recommended on vital indications only. EchoCG was used in 155 patients with cholecystitis, 131 of them were operated. 2 (1.52%) patients died due to acute cardio-vascular insufficiency and pulmonary artery thromboembolism.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

目的 评价脊髓胶质细胞在小鼠骨癌痛形成中的作用.方法 健康雄性C3H/He小鼠40只,周龄8～10周,体重18～22 g,随机分为4组(n=10):假手术组(S组)、骨癌痛组(B组)、PBS组(P组)和米诺环素组(M组).S组跟骨骨髓腔内注射PBS 10 μl;余3组跟骨骨髓腔内注射含2×105个骨纤维肉瘤细胞的PBS 10 μl制备骨癌痛模型,于造模前即刻开始PBS组鞘内注射PBS 5μl,M组鞘内注射米诺环素(用PBS溶解为0.2 mmol/L)5μl,1次/d,连续11 d.于造模前1 d、造模后即刻、3、5、7、9、11 d时测定机械痛阈;于造模后3、7、9、11 d机械痛阈测定结束后测定冷痛阈.痛阈测定结束后处死小鼠,取脊髓组织,测定神经胶质纤维酸性蛋白(GFAP)和CD11b的表达水平.结果 与S组比较,B组和P组造模后3-11 d时、M组造模后3、5 d时机械痛阈升高,B组、P组和M组造模后7～11 d时冷痛阈升高,脊髓CD11b和GFAP表达上调(P<0.05).与B组比较,M组造模后3-11 d时机械痛阈降低,造模后7-11 d时冷痛阈降低,脊髓CD11b和GFAP表达下调(P<0.05).结论 脊髓胶质细胞(星形胶质细胞和小胶质细胞)的激活参与了小鼠骨癌痛的形成.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.     

脊髓胶质细胞在小鼠骨癌痛形成中的作用

Objective To evaluate the role of gliocyte in the spinal cord in the development of bone cancer pain (BCP) in mice. Methods Forty male C3H/He mice aged 8-10 weeks weighing 18-22 g were randomly divided into 4 groups ( n = 10 each) : group I sham operation (group S) , group II BCP, group Ⅲ PBS and group IV minocyline (group M) . In group BCP, PBS and M, bone cancer pain was produced by injection of NCTC2472 fibrosarcoma cell suspension (2 x 105 cells) 10 μl into medullary cavity of calcaneus bone, while in group S, PBS solution 10 μl was injected instead of cancer cell suspension. In group PBS and M, PBS 5 μl and minocyline 5 μl (dissolved to 0.2 mmol/L in PBS)_were given IT immediately before cancer cell inoculation once a day for 11 consecutive days respectively. Mechanical pain threshold was measured at 1 d before cancer cell inoculation, and at 0, 3, 5, 7, 9 and 11d after cancer cell inoculation. Cold pain threshold was measured at 3, 7, 9 and 11d after cancer cell inoculation. The animals were killed after measurement of pain threshold and L4-6, segment of spinal cord was removed for determination of GFAP and CD11b expression by Western blot. Results Compared with group S, mechanical pain threshold was significantly increased at 3-11 d after cancer cell inoculation in group BCP and PBS, and at 3 and S d after cancer cell inoculation in group M, and cold pain threshold was significantly increased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was up-regulated in group BCP, PBS and M ( P < 0.05) . Compared with group BCP, mechanical pain threshold was significantly decreased at 3-11 d after cancer cell inoculation, cold pain threshold was significantly decreased at 7-11 d after cancer cell inoculation, and expression of CD11b and GFAP was down-regulated in group M ( P <0.05) . ConclusionThe activiton of gliocyte in the spinal cord is involved in the development of bone cancer pian in mice.