金诃伽维胶囊的毒理学安全性评价

目的:对金诃?伽维胶囊的食用安全性进行毒理学评价。方法:依据食品安全性毒理学评价程序和方法进行急性毒性试验、遗传毒性试验(Ames试验、骨髓细胞微核试验和精子畸形试验)、大鼠30 d喂养试验。结果:小鼠急性毒性试验经口最大耐受剂量(MTD)均10g/kg·bw,属无毒级。Ames试验、骨髓细胞微核试验和精子畸形试验结果均为阴性,表明受试物无致突变作用,30d喂养试验,试验动物均生长发育良好,体重、摄食量、血液学、血液生化学、脏器系数及病理组织学相关指标均未见异常变化。结论:金诃?伽维胶囊急性毒理分级属无毒级,无遗传毒性,在本实验剂量范围内,金诃?伽维胶囊属于安全性保健食品。     

大豆多肽的毒性研究

目的研究大豆多肽的毒性。方法小鼠急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、大鼠30d喂养试验。结果Ames试验、微核试验和精子畸形试验结果均为阴性;大鼠30d喂养试验结果显示该样品30d喂养对大鼠各项观察指标未见毒性作用。结论在本次实验条件下,大豆多肽为无毒物质,未显示有遗传毒性和亚急性毒性作用。     

藏虫草胶囊毒理学安全性评价

目的:评价藏雪玛牌虫草胶囊的安全性。方法:依据食品安全性毒理学评价程序和方法进行急性毒理、骨髓细胞微核、精子畸形、Ames和30 d喂养实验。结果:小鼠经口最大给药量>24 g·kg-1,属实际无毒级;骨髓细胞微核、精子畸形和Ames实验结果均为阴性;30 d喂养实验中各项指标均未见异常。结论:藏雪玛牌藏虫草胶囊是较安全的。     

洋葱胶囊安全性评价及辅助降血压和降血脂功能研究北大核心CSCD

目的评价洋葱胶囊的安全性及研究其辅助降血压和降脂功能。方法实验操作于2012年,是依据卫生部《保健食品检验与评价技术规范》(2003年版),进行小鼠急性经口毒性试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验、Ames试验、30 d喂养试验、辅助降血压功能检测和辅助降血脂功能试验。结果洋葱胶囊小鼠经口MTD均大于15 g/kg·bw,属无毒级;对鼠伤寒沙门氏菌TA97、TA98、TA100和TA102菌株无致突变作用;对小鼠骨髓嗜多染红细胞未见致突变作用;对小鼠精子未显示损伤作用;在0.5~10 g/kg·bw剂量范围内,大鼠连续喂饲30 d后,未发现明显损害作用,最大未观察到有害作用剂量为10 g/kg·bw;连续4周经口给予自发高血压大鼠(SHR)洋葱胶囊(3 000 mg/kg·bw)后,其血压明显降低,且在相同剂量下,洋葱胶囊对正常大鼠血压未见影响;连续30 d给予大鼠3 000 mg/kg的洋葱胶囊后,血清总胆固醇(TC)和甘油三酯(TG)均明显低于高脂对照组(P<0.05)。结论对洋葱胶囊毒理学安全性评价检测结果表明洋葱胶囊在受试剂量范围内未见毒性作用,实验表明洋葱胶囊具有辅助降血压和辅助降血脂功能。本研究为洋葱胶囊的开发应用提供依据,也为洋葱保健功效的机制研究提供了数据支持。     

百草胶囊的毒性研究

目的　研究百草胶囊的毒性。方法　采用最大耐受剂量 (MTD)试验 ,小鼠骨髓嗜多染红细胞微核试验 ,小鼠精子畸形试验 ,Ames试验和大鼠 30d喂养试验 ,分别观察百草胶囊的急性毒性 ,遗传毒性和亚急性毒性。结果　百草胶囊对小鼠经口MTD大于 16 0 0 0mg/kg ;对小鼠骨髓嗜多染红细胞微核试验无诱发微核增多作用 ;小鼠精子畸形试验未见导致精子畸形和畸形率增高 ;Ames试验在加与不加S9的条件下均无致突变性 ;大鼠 30d喂养试验未观察到中毒表现 ,百草胶囊各剂量组动物体重 ,食物利用率 ,血液学和血液生化学指标值 ,各脏器的脏 /体比值与空白对照组比较差异均无显著意义 (P >0 0 5 )。主要脏器在外观形态和组织学上均无异常变化。结论　百草胶囊对动物的生长发育、造血功能、肝肾功能、器官组织均无明显毒性。     

洋葱胶囊安全性评价及辅助降血压和降血脂功能研究

目的评价洋葱胶囊的安全性及研究其辅助降血压和降脂功能。方法实验操作于2012年,是依据卫生部《保健食品检验与评价技术规范》(2003年版),进行小鼠急性经口毒性试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验、Ames试验、30 d喂养试验、辅助降血压功能检测和辅助降血脂功能试验。结果洋葱胶囊小鼠经口MTD均大于15 g/kg·bw,属无毒级;对鼠伤寒沙门氏菌TA97、TA98、TA100和TA102菌株无致突变作用;对小鼠骨髓嗜多染红细胞未见致突变作用;对小鼠精子未显示损伤作用;在0.5~10 g/kg·bw剂量范围内,大鼠连续喂饲30 d后,未发现明显损害作用,最大未观察到有害作用剂量为10 g/kg·bw;连续4周经口给予自发高血压大鼠(SHR)洋葱胶囊(3 000 mg/kg·bw)后,其血压明显降低,且在相同剂量下,洋葱胶囊对正常大鼠血压未见影响;连续30 d给予大鼠3 000 mg/kg的洋葱胶囊后,血清总胆固醇(TC)和甘油三酯(TG)均明显低于高脂对照组(P0.05)。结论对洋葱胶囊毒理学安全性评价检测结果表明洋葱胶囊在受试剂量范围内未见毒性作用,实验表明洋葱胶囊具有辅助降血压和辅助降血脂功能。本研究为洋葱胶囊的开发应用提供依据,也为洋葱保健功效的机制研究提供了数据支持。     

驱铅益智口服液的安全性毒理学评价

目的通过动物实验初步探讨驱铅益智口服液（口服液）的安全性,旨在开发一种安全有效的驱铅产品。方法依据《食品安全性毒理学评价程序和方法》的要求,采用霍恩氏法进行急性毒性试验,应用Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验和30d喂养试验进行遗传毒性和安全性评价。结果急性毒性试验显示小鼠经口的LD50〉10g·kg^-1·bw^-1;3种遗传毒性试验均显示口服液组与阴性对照纽相比差异均无统计学意义（P〉0．05）;30d喂养试验期间大鼠一般状况、体重、食物利用率、外周血常规和生化指标、脏体比均与阴性对照组相比差异均无统计学意义（P〉0．05）。光镜下病理学检查组织结构均未见异常。结论从食品毒理学角度出发,可以认为口服液无毒、无致突变作用。     

番茄红素胶囊的毒理学安全性评价

目的探讨番茄红素胶囊的安全性。方法安全性毒理试验按《食品安全性毒理学评价程序和方法》进行。结果小鼠急性毒性试验LD50〉18.4 g/kg BW,属无毒级;10.0、5.0、2.5 g/kg BW三个剂量的小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验均未见染色体畸变作用;Ames试验8、40、200、1000、5000μg/皿5个剂量加与不加S9,均未呈现基因突变作用。大鼠30 d喂养试验未观察到的有害作用剂量为1.67 g/kg BW。结论番茄红素胶囊安全无毒、无致突变性。     

汝康胶囊毒理学安全性实验研究

检测汝康胶囊作为保健食品的安全性.方法:依据卫生部<保健食品检验与评价技术规范>[1],通过小鼠经口急性毒性试验、致突变试验,大鼠30天喂养试验对汝康胶囊进行检测.结果:小鼠经口LD50＞21.5g/kg·bw,属无毒级物质;致突变试验结果为阴性;大鼠30天喂养试验表明各剂量组体重增长、食物利用率、脏体比、血液学及血清...     

橙汁粉末香精毒理学研究及安全性评价

目的研究橙汁粉末香精的毒性和安全性。方法按照《食品安全毒理学评价程序和方法》进行试验。结果橙汁粉末香精对小鼠急性毒性实验LD50=9600mg·kg-1,属实际无毒级;通过Ames试验、骨髓细胞微核试验、小鼠精子畸形试验;传统致畸实验,发现橙汁粉末香精无遗传毒性;30天喂养实验以及90天喂养实验,发现对动物的体重、血液常规及血清生化指标均无明显毒性作用(P>0.05)。结论根据以上结果可基本断定橙汁粉末香精是一种安全、无毒的食品、药品矫味剂。     

薏苡仁多糖急性毒性及遗传毒性试验研究

目的了解薏苡仁多糖的毒理学安全性。方法小鼠急性毒性试验和遗传毒性试验（Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验）。结果薏苡仁多糖的小鼠经口LD50〉20g·kg^-1;在Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验中均呈阴性反应,未显示有遗传毒性作用。结论薏苡仁多糖基本无毒性。     

Safety evaluation of short-term exposure to chitooligomers from enzymic preparation.

Chitooligomers have significant application to the development of functional foods. This paper evaluated systematically the safety of chitooligomers, which were prepared by enzymatic depolymerization of chitosan. The oral maximum tolerated dose of this chitooligomes was more than 10 g/kg body weight in mice. It had no mutagenicity judged by negative experimental results of Ames test, mouse bone marrow cell micronucleus test and mouse sperm abnormality test. A 30-day feeding study shows that no abnormal symptoms and clinical signs or deaths were found in rats during the test. There were no significant difference in body weight, food consumption and food availability of rats in each test group. No significant differences were found in each hematology value, clinical chemistry value and organ/body weight ratio, either. No abnormality of any organ was found during histopathological examination. It is concluded that short-term ingestion of chitooligomers is of non-toxicity.     

Safety evaluation of a triterpenoid-rich extract from bamboo shavings.

Triterpenoids, which may have significant application to the development of natural medicines and functional foods as biological active components, are widely distributed throughout the plant kingdom. This paper evaluated the safety of a triterpenoid-rich extract of bamboo shavings (EBS) systematically. (i) Acute toxicity test: The oral maximum tolerated dose of EBS was more than 10 g/kg body weight both in rats and in mice, due to the absence of toxicity according to the criteria of acute toxic classifications. (ii) Mutagenicity test: It had no mutagenicity judged by negative experimental results of Ames test, mouse bone marrow cell micronucleus test and mouse sperm abnormality test. (iii) 30 days feeding study: No abnormal symptoms and clinical signs or deaths had been found in rats in each group during the test. No significant difference had been found in body weight, food consumption and food availability of rats in each test group (P>0.05). In addition, no significant differences were found in each hematology value, clinical chemistry value and organ/body weight ratio, either (P>0.05). No abnormality of any organ was found during histopathological examination. It can be concluded that the extract of bamboo shavings is of low toxicity and support the use of EBS for various foods.     

Safety evaluation of protein of silkworm (Antheraea pernyi) pupae.

The protein of silkworm pupae (PSP) has been thought to be a new available source of high quality protein that contains all the amino acids needed by the human body. The safety of PSP was evaluated systematically by a series of acute and sub-acute toxicological tests: (i) Acute toxicity test: The oral maximum tolerated dose of PSP was more than 15.0 g/kg body weight in mice, due to the absence of toxicity according to the criteria of acute toxic classifications; (ii) Mutagenicity test: PSP had no mutagenicity, as judged by a negative Ames test, mouse bone marrow cell micronucleus test and mouse sperm abnormality test; (iii) 30 days feeding study: No deaths or abnormal hematological, clinical chemical and histopathological changes and clinical signs had been found in rats when administrated PSP at 0.30, 0.75 and 1.50 g/kg/day to the rats for 30 days in each group during the test, respectively. No statistically significant differences had been found in body weights, food consumption and food efficiency of rats in each test group (P>0.05). These results indicate that PSP can be generally regarded as safe at a maximum dose of 1.50 g/kg/day in rats.     

葡萄糖酸铜的毒性评价

通过葡萄糖酸铜的食品安全性评价表明,葡萄糖酸铜是中等急性毒性(LD_(50)为41mg/kg)和弱蓄积毒性的化学物质。在小鼠骨髓微核试验和小鼠精子畸变分析中,葡萄糖酸铜均显示阴性结果,并且在Ames试验中对鼠伤寒沙门氏菌株也无基因突变作用。     

桑黄发酵多糖胶囊急性毒性和遗传毒性研究

本文通过急性毒性试验、小鼠精子畸形试验和骨髓微核试验,研究桑黄发酵多糖胶囊的急性毒性和遗传毒性的影响。试验结果表明,小鼠对桑黄发酵多糖胶囊的最大耐受量大于20g/kg,与空白组相比,胶囊组小鼠的精子畸形率和骨髓细胞微核率无显著性差异。结果表明,桑黄发酵多糖胶囊属实际无毒物质,无遗传毒性。