高效液相色谱测定大鼠早孕组织多胺的含量

目的：建立大鼠早孕组织中发含量ＨＰＬＣ测定的方法。方法：大鼠早孕组织中多胺用１０％三氯乙酸提取，苯甲酰氯柱前衍生，反相ＨＰＬＣ测定。结果：多胺中腐胺、精脒和精胺能较好分离；腐胺检测限可达１ｎｍｏｌ、精脒和精胺可达５ｎｍｏｌ；腐胺在１￣１０００ｎｍｏｌ、精脒和精胺在５￣１０００ｎｍｏｌ范围内线性良好；腐胺、精脒和精胺平均生物样本回收率分别为９５．８％、９２．８％和８１．７％，日内变异数（ＣＶ％）分别     

胆管癌精脒免疫组织化学研究

胆管癌精脒免疫组织化学研究迟晶，庄宝珠，沙文阁（第一临床学院消化内科）关键词精脒；胆管癌；免疫组织化学多胺是含有两个或两个以上氨基的长链脂肪族含氮化合物。一般指腐胺、精脒和精胺。腐胺是它们的生理性前体，经过脱羧生成精脒和精胺。多胺分布于动植物的细胞体...     

妇科肿瘤病人尿中精胺精脒的含量测量

恶性肿瘤病人尿中多胺(腐胺、精胺、精脒)含量较健康人为高,已有报道。但妇科良恶性肿瘤或炎症病人尿多胺的含量测定尚未见报道,本文测定了妇科良恶性肿瘤病人一次晨尿中精胺和精脒的含量。1 材料和方法1.1 对象恶性肿瘤30例,其中宫颈癌10例,卵巢癌11例,子宫内膜癌4例,子宫肉瘤2例,其它恶     

多胺与缺血性脑血管疾病研究进展

多胺是指自然界中存在的二胺、三胺、四胺,属天然脂肪族多价正离子性胺类物质,主要包括腐胺、精脒和精胺[1].多胺最初由Anonie van Leeuwenhoek于1678年在人类的精液中发现.生理pH值时,多胺的氨基带正电荷,其结合能力随电荷的增加而加强(PUT＜SPD＜SPM).多胺广泛分布于真核细胞生物,包括脊椎动物的中枢神经系统. 机体各部分多胺含量差异很大,脑组织中不同部位的多胺含量也不相同,并且腐胺水平较精脒和精胺要低很多.精脒在伏隔核、小脑皮质、大脑皮质第Ⅰ层、海马、下丘脑、中脑中缝核、杏仁中央核和外侧核以及脑室周围组织分布最多[2].     

正常外周血细胞中的多胺分布

本文应用氨基酸自动分析仪测定了18例正常人外周血的红细胞、淋巴细胞(包括单核细胞)和粒细胞的多胺浓度。以nmol/10~9细胞为单位,其白细胞的腐胺、精脒与精胺的浓度要高出红细胞几百倍,然而淋巴细胞与多形核细胞之间则不存在显著差异,而且性别之间亦无差异。不同年龄组血细胞多胺测定发现,淋巴细胞中的精脒、精胺水平有随年龄增长而减低的趋势,而多形核细胞中的精脒、精胺则有随年龄增加而增加韵趋势,这些均可能与细胞的功能减退有关。     

高效液相色谱法快速测定脑梗塞模型大鼠脑组织中多胺的含量

目的:建立一种简便、灵敏和快速分离测定大鼠脑组织中腐胺、精脒和精胺等多胺含量的高效液相色谱法。方法:采用苯甲酰氯作为衍生化试剂,以1,6-己二胺为内标,采用polygocyl(250×4.6 mm 5μm)C18色谱柱,流动相为甲醇-水(62:38 V/V),流速1.0 mL/min,检测波长229 nm。结果:本法线性范围是1～2 000/mL,回收率大于90%。日内和日间变异系数为1.5%～4.2%。最低定量浓度为1 nmol/mL。结论:该方法符合生物样品分析要求,为脑组织中多胺的含量测定提供了一种简便、准确、灵敏度高的分析方法。     

细胞培养液中微量多胺的高效液相色谱荧光检测法

先采用Ｃ１８样品预处理小柱浓缩培养液中的微量多胺，经ＤＮＡ－Ｃｌ荧光衍生，高效液相色谱分离，荧光检测，结果可使腐胺、精脒、精胺等组份得到完全分离。精密度批内ＣＶ分别为２．４９％、２．９５％；批间ＣＶ为４．４９％４．６９％、５．１６％；回收率为１０３．６％、１０１．６％、９９．０％。我们发现，许多常用人工合成培养基中均含有极低浓度的多胺，本法可分析培养细胞前后培养基中微量多胺的变化，为研究多胺对细胞     

妇科肿瘤病人尿中精胺精脒测定

恶性肿瘤病人尿中多胺(腐胺、精胺、精脒)含量高于健康人,国内外均有报道。本文测定了妇科恶性肿瘤30例,良性肿瘤20例,健康妇女33例一次晨尿中精胺与精脒含量,报告如下。1 方法与对象参考Inoue与范慕贞的方法,将尿液进行酸水解、离子交换柱层析提取、沸水浴蒸干、高压纸电泳分离、茚三酮显色、分光光度计比色定量测定。肌酐     

细胞培养液中微量多胺的高效液相色谱荧光检测法

先采用Ｃ１８样品预处理小柱浓缩培养液中的微量多胺，经ＤＮＳ－Ｃｌ荧光衍生，高效液相色谱分离，荧光检测，结果可使腐胺、精脒、精胺等组份得到完全分离。精密度批内ＣＶ分别为２．４９％、２．９５％、４．２６％；批间ＣＶ为４．２９％、４．６９％、５．１６％；回收率为１０３．６％、１０１．６％、９９．０％。我们发现，许多常用人工合成培养基中均含有极低浓度的多胺，本法可分析培养细胞前后培养基中微量多胺的变化，为研究多胺对细胞增殖的调控作用提供了有用的手段。     

高效液相色谱法测定血清中的多胺

高效液相色谱法测定血清中的多胺石南宁贡小清童正本（南京铁道医学院预防医学系南京２１０００９）多胺包括腐胺、精脒、精胺，是一类低分子量的脂肪族化和物，在细胞生长繁殖过程中起重要作用。研究发现，癌症患者血、尿中多胺含量较正常人明显增高，而当肿瘤切除或病情...     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.