高效液相色谱法测定血浆中依托泊甙

依托泊甙(足叶乙甙,Vp 16-213)是半合成的鬼臼毒衍生物,化学名为4-脱甲基表鬼臼毒9-[4,6-O-亚乙基-β-D-吡喃葡萄糖甙],具有广谱抗肿瘤活性,能抑制DNA和蛋白质的合成。但临床应用时须进行血药浓度的监测,以提高疗效,降低毒副反应。文献曾报道以氚标法及高效液相色谱法测定血药浓度。本文采用后法,并作了较大的改进。     

抗肿瘤药物依托泊甙的质量研究

本文采用溶剂重结晶法精制抗肿瘤药物依托泊甙，精制前后用ＵＶ、ＩＲ及ＴＬＣ进行检查；用相溶度分析法和高效液相色法测定含量；用热分析方法测定了依托泊甙的热特征。精制后的依托泊甙纯度达９９％以上。     

HPLC法测定人血浆中的依托泊甙

目的：采用高效液相色谱法测定人血浆中依托泊甙（鬼臼乙叉甘，VP-16），方法：以甲醇-水（51：49）作流动相，UV232nm检测，外标法定量，结果：血浆中VP-16平均回收率为99.36%。最低检出浓度50μg/ml，精密度日内RSD=4.88%。日间RSD=5.46%。     

依托泊甙复乳的理化性质与体外释放

测定了依托泊甙复乳的显微结构、粒经分布、渗透压、电学和流变学性质。体外释放试验显示本复乳有快、慢两个释放相,10h 和48h 时释药分别为50%和88%,表明具有缓释作用。用零级(Q-t)和 Higuchi模式(Q-t~(1/2))对复乳的体外释放数据进行拟合,相关系数分别为0.9027、0.9803,表明其体外释放主要以扩散形式进行。     

高效液相色谱法检测人血浆中的依托泊甙

本文指出一种地球化学闭合模型的热中子瞬发活化伽马分析方法，它是地球化学测井的核心技术，而标准谱的获得、基于标准谱的加权最小二乘解谱方法、灵敏度刻度及地球化学闭合模型是这一方法的关键环节。我们在模拟井场条件下，对这一方法进行了实验研究。用Ｎａｌ（Ｔ１）探测器获得了Ｆｅ，ＳＩ，Ｃｌ，Ｈ等元素的标准谱，并进行解说拟合和灵敏度刻度；同时，用高纯锗探测器进行了分析对照。     

依托泊甙复乳处方设计和稳定性考核

通过对油的品种、乳化剂及 HLB 值、稳定剂等辅料的筛选,并经正交设计试验获得较稳定的 W_1o/w_2型依托泊甙复乳处方和制备工艺条件,测定了药物包裹率并考核了稳定性。     

依托泊甙诱发心房纤颤1例

<正> 患者女性,45yr,医师,主诉右乳癌术后右肺门淋巴腺转移,左髋关节周围转移,于1987年12月入院。以往身体健康,无高血压、冠心病、风心病、心房纤颤(下简称房颤)等病史。入院时检查:一般情况好,左腿跛行,心率82次/min,律齐,心界不大,未闻及器质性杂音。两肺听诊阴性,腹部检查阴性,心电图正常,X线示右肺门影浓密,左髋臼上缘髂骨有溶骨性骨质破坏。在用环磷酰胺、长春新碱、甲氨蝶呤(COM)方案治疗时,除有厌食、厌油等消化道症状外,余无特殊不适。上药连用2wk,停用1wk后,改用依托泊甙(etoposide,足叶乙甙,鬼臼乙叉甙)100mg加入5％葡萄糖液500mL静滴,qd,连     

家兔静注依托泊甙的药物动力学研究

采用改良的ＨＰＬＣ法测定家兔血清中的依托泊甙，同时检测到两个代谢物，基本确定为顺依托泊甙和反依托泊甙羟基酸。家兔静注依托泊甙的药物动力学符合二室开放模型，其主要参数为：Ｔ１／２α＝３．００±１．９１ｍｉｎ，Ｔ１／２β＝５８．９３±４５．３５ｍｉｎ，Ｖｃ＝０．３８±０．２５Ｌ／ｋｇ，Ｃｌ＝０．０２±０．０１Ｌ·ｋｇ－１·ｍｉｎ－１，ＡＵＣ＝７４１．４０±３８２．６０μｇ·ｍｌ－１·ｍｉｎ－１。     

依托泊甙联合化疗治疗非霍奇金淋巴瘤疗效评价

依托泊成（VP－16）取代治疗非霍奇金淋巴瘤（NHL的CHOP方案中的长春新碱引出了一项改良方案四CHEP。这是近年来的一个新趋势。依托泊成单药治疗NHL的有效率约30％左右，而且无神经毒性，我科疾CHEP方案治疗了28例NHL，现报告如下。1材料与方法1．1病例选择1．1．l本组病例均经     

抗癌活性成分满五酸等三个化合物的分离和鉴定

从产于四川宜宾翼梗五味子果实中,分得抗癌活性成分满五酸(1),同时分得schizandronic acid(2)和cuparenic acid(3),结构由与标准化合物比较光谱数据和物理常数而确证。     

鸡嘴簕的化学成分分离和鉴定

目的 对鸡嘴簕枝叶的化学成分进行分离和鉴定。方法 利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等现代多种色谱技术进行分离纯化,利用核磁共振等波谱技术和文献对照的方法进行结构鉴定。结果从鸡嘴簕枝叶中分离鉴定了12个化合物,分别为caesaloside A （1）,2β-ethoxyclovane-9α-ol （2）,2β-methoxyclovan-9α-ol （3）,8（17）,13-ent-labdadien-15→ 16-lactone-19-oic acid （4）,高根二醇（5）,3-甲氧基槲皮素（6）,槲皮素（7）,芹菜素（8）,木犀草素（9）,3,5-二羟基-4-甲氧基苯甲酸乙酯（10）,没食子酸乙酯（11）和没食子酸（12）。结论 化合物1 和2 为新化合物,其余10个化合物皆为首次报道从该植物中分离得到。     

茶多酚的提取分离和分析鉴定研究

概述国内外对茶多酚的提取分离和分析鉴定的研究，并结合工作实践对这些方法进行评估。重点介绍近年来茶多酚的提取、分离、分析和鉴定的新技术、新方法、新工艺、新仪器和新设备，以期为茶多酚的生产与研究提供参考。     

Bioassay-Guided Isolation,Identification of Compounds from Sterculia diversifolia and Investigation of Their Anti-Glycation and Antioxidant Activities

Pharmaceutical Chemistry Journal - The current study was conducted to isolate and identify bioactive compounds from Sterculia diversifolia G. Don and to investigate their anti-glycation and...     

西藏胡黄连中两个新化合物的鉴定

运用大孔吸附树脂、硅胶及葡聚糖凝胶等柱色谱技术对西藏胡黄连（Picrorhiza scrophulariiflora Pennell）根茎水提液的氯仿、乙酸乙酯及正丁醇部位进行分离纯化，得到17个单体化合物，并通过IR、MS、NMR等波谱技术推断化合物的结构。结果表明其中2个为新的苷类化合物，8个化合物为首次从该植物发现。     

The tumour specific pro-apoptotic factor apoptin (Vp3) from chicken anaemia virus

Cancer is a growing problem for human health world-wide. Dramatic breakthroughs have increased our understanding of the molecular mechanisms involved in the process of tumorigenesis, allowing us to develop more refined anti-cancer treatments, expanding the repertoire of available anti-cancer drugs, and increasing the efficiency of their delivery to malignant cells. Nevertheless, even with improved understanding of the complex origins of cancer cells, there is a dearth of efficient and above all specific anti-cancer treatments. Apoptin (viral protein 3 - VP3), a gene product derived from the Chicken Anaemia Virus (CAV) represents a novel anti-cancer tool. It appears to have innate tumour-specific p53-independent, Bcl-2-enhanced pro-apoptotic activity, and hence may be of great utility in the endeavour to achieve specific and efficient elimination of cancer cells, particularly in cases of drug resistance through Bcl-2 overexpression/loss of p53 function etc. This review will examine the unique aspects of apoptin's properties, and in particular, its ability to localise specifically in the nucleus of transformed but not normal cells. The latter ability, importantly, appears to be integrally related to its tumour-specific pro-apoptotic action.     

Estimation of Impurity Profiles of Drugs and Related Materials. 12. Isolation and Identification of an Isomeric Impurity in Danazol

We report on a new isomeric impurity of danazol. This impurity designated as isodanazol was detected by reversed-phase high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Its structure was determined after separation by preparative HPLC. Mass spectrometry revealed the isomeric nature of the impurity while the UV spectrum indicated profound difference in the isoxazole moieties. The structure of the isomeric isoxazole ring in isodanazol was determined by NMR spectroscopy using COSY, HETCOR and NOE measurements. The difference between the U V spectra of danazol and isodanazol is explained on the basis of the difference between the aromaticities of their isoxazole rings supported by quantum chemical calculations. The quantitative determination of the impurity down to the 0.05% level can be performed by HPLC, gas chromatography and TLC densitometry.