A novel HLA-B*15 allele, B*9524, identified by sequence-based tying in the Chinese population

We report here the identification of a novel human leukocyte antigen (HLA)-B*9524 allele that was detected by polymerase chain reaction sequence-based typing.     

A novel HLA-B*46 allele, B*4609, identified by sequence-based typing in a Chinese donor

A novel human leukocyte antigen-B allele, B*4609, has been identified. The B*4609 allele has one nucleotide change from the closest matching allele B*460101 resulting in an amino acid change from E (GAG) to V (GTG) at codon 176.     

A novel HLA-B*54 allele, B*5417, identified in a Northern Chinese Han individual

The full-length sequence of HLA-B*5417 differs from HLA-B*5401 only by single-nucleotide change at nt 709 where A→C resulting in a amino acid substitution from Ile (ATC) to Val (GTC) at codon 213 in exon 4.     

Characterisation of a new HLA-B allele, HLA-B*0720, identified in the Cuban population

The HLA class I genes display significant levels of polymorphism, which is principally due to hypervariable regions located in the second and third exons. To date, 286 HLA-B alleles have been identified and characterised. We describe a new HLA-B*07 allele present in a Cuban Caucasoid individual, which has been officially named HLA-B*0720.     

Identification of a new HLA-B*78 allele in a Hispanic family

A new B*78 variant, B*7804, was detected in three members of a Hispanic family. The novel B*78 sequence differs from B*78021 by two substitutions: T at nucleotide 527 (all other B*78s have A) and T at nucleotide 583 (all other B*78s have a C). Both nucleotide substitutions encode amino acid changes at codons 152 and 171, respectively.     

Description of a new HLA-B allele: HLA-B*4077

HLA-B*4077 differs from B*400101, B*4036, and B*401401 in five, four, and three nucleotides, respectively. However, amino acid alignment would indicate a closer relationship with B*4036 allele, showing a single amino acid replacement at codon 131 (R>S).     

Identification of a novel HLA-B allele, B*460102, in three Taiwanese individuals

We here report a new human leukocyte antigen (HLA)-B allele, B*460102, which differs from B*460101 by synonymous substitution at the third nucleotide of codon 74 (GAC→GAT) and independently found in three Taiwanese individuals.     

A novel HLA-B*15 allele, B*9534, identified by sequence-based typing in the Chinese population

We report here the identification of a novel human leukocyte antigen-B*9534 allele that was detected by polymerase chain reaction sequence-based typing.     

A novel HLA-B*40 allele, HLA-B*4083, identified in a Korean individual

HLA-B*4083 differs from the closest aligned sequence HLA-B*400601 by three nucleotide substitutions at codons 31 (ACG →ACC), 32 (CTG →CAG), and 41 (ACG → GCG), resulting in two amino acid changes at residues 32 (Leu to Gln) and 41 (Thr to Ala).     

A new HLA-B*51 variant, B*5158, identified by sequence-based typing in a Korean individual

A novel human leukocyte antigen (HLA)-B*51 allele, officially named HLA-B*5158, was identified in the cord blood from Korean. HLA-B*5158 allele shows single nucleotide difference from B*510101 in exon 2 at nucleotide position 214 (C/T), resulting in an amino acid substitution, Trp48Arg.     

Identification of a novel HLA-B46 allele, B*4602, in Japanese

The B*4602 was identified in a healthy Japanese donor. The B46 variant antigen differed serologically from B46. The nucleotide sequence of B*4602 differed from that of B*4601 by a single base (from A to G) at position 293 in exon 2. The substitution changed the amino acid Asp at position 74 to Gly. The gene frequency of B*4602 in the Japanese population is extremely low. The presumed haplotype of B*4602 was HLA-A2-Cw1-B*4602-DRB1*09. This is the first report of polymorphism of the B46 antigen.     

A new HLA-B*40 allele, HLA-B*4085, identified by sequence-based typing in the Korean population

A new HLA-B*4085 showed one nucleotide difference from B*400201 at the position 500 of exon 3, resulting in a coding change from Thr to Ile (T143I).     

Identification of three novel HLA class I alleles: HLA-B*3928, HLA-B*400104 and HLA-B*4437

Three new human leukocyte antigen (HLA) class I alleles have been identified in the Tissue Typing Laboratory in Sydney, Australia. Sequence analysis of exon 2 and exon 3 of the HLA-B gene revealed the novel polymorphism. A silent substitution of C to T at nucleotide position 369 has been identified for the HLA-B*400104 allele when compared to the closest matched allele, HLA-B*400101. The HLA-B*3928 allele was identified with a nucleotide substitution of G to C at position 362 when compared to the closest matched allele, HLA-B*390101, resulting in an amino acid substitution of Arginine to Threonine. A nucleotide substitution of C to G at position 572 resulting in the amino acid change Serine to Tryptophan was identified in the new allele HLA-B*4437, when compared to the closest matched allele HLA-B*440301. Both amino acid substitutions implicate a different specificity and affinity of antigen binding for the alleles HLA-B*3928 and HLA-B*4437.     

A new HLA-B44 variant (B44BO [B*4408]) identified by serology

Using HLA serology, we detected a new variant of HLA-B44 — B44BO — in two families. This antigen reacts with B44 antisera and is negative with over one-third of B12 (B44, B45) sera but reacts with 50% of antisera with a B62 component, especially if they contain anti-B57. The variant, B*4408, differs from the common B*4402 by 4 nucleotide substitutions in exon 2: 193, 206 and 209, which produce changes in the α1domain at positions 41, 45 and 46 (TKE in B*4402 and AMA in B44BO); and nucleotide 213, a silent substitution. At each of these positions, B*4408 is identical to B*46, B*57 and many B*15 alleles. As anticipated from its predicted iso-electric point (5.71), one-dimensional isoelectric focusing studies showed that B44BO focuses at the same position as B*4402. The sequence and serological reactivity of this rare antigen allowed the identification of two likely epitopes shared by two different groups of HLA-B antigens.