高危型HPV感染对妊娠期影响的探讨

目的 探讨高危型人乳头瘤病毒(HPV)对妊娠及胎儿的影响.方法 选取HC-Ⅱ分型检测法检测的62例高危型HPV感染孕妇以及同时期的62例非感染健康孕妇作为研究对象.比较分析妊娠早期、中期的流产率、胎膜早破率,以及妊娠晚期的早产率、胎儿发育迟缓率、死胎率、畸形率、新生儿窒息率等.结果 结果显示,感染组妊娠早、中、晚期不良妊娠结局发生率(22.6％、14.5％、33.9％)均高于对照组(1.6％、1.6％、8.1％)(P＜0.05).结论 HPV的感染并非只通过性传播,还可能通过垂直传播,并且对妊娠和胎儿产生一定的负面影响.     

HIV感染伴尖锐湿疣的HPV分型特点探讨

目的 探讨人免疫缺陷病毒(HIV)感染伴尖锐湿疣(CA)的人乳头瘤病毒(HPV)分型特点。方法 544例尖锐湿疣(CA)患者,根据HIV检测是否阳性分为HIV阳性组(261例)和HIV阴性组(283例)。检测两组患者的HPV分型。比较两组患者HPV低危型、高危型检出情况, HPV各亚型分布情况, HPV单一感染和多重感染情况。结果 HIV阳性组患者HPV高危型检出率57.1%高于HIV阴性组的30.0%,低危型检出率24.1%低于HIV阴性组的51.2%,差异具有统计学意义(P<0.05);两组HPV高危型合并低危型检出率比较差异无统计学意义(P>0.05)。HPV低危型HPV11在HIV阳性组和HIV阴性组患者中的检出率均最高, HIV阳性组HPV11检出率56.7%明显高于HIV阴性组的37.8%,差异具有统计学意义(P<0.05);HPV高危型HPV16在HIV阳性组和HIV阴性组患者中的检出率均最高,其次为HPV58, HIV阳性组HPV16、HPV58检出率分别为28.0%、17.6%,明显高于HIV阴性组的18.0%、11.7%,差异具有统计学意义(P&l...     

超高频电波刀治疗高危人乳头瘤病毒感染合并宫颈低度鳞状上皮内瘤变的临床研究

目的 探讨超高频电波(LEEP)刀治疗高危人乳头瘤病毒(HPV)感染合并宫颈低度鳞状上皮内瘤变(LSIL)的临床疗效.方法 回顾性分析2007年12月至2011年12月我院门诊检查发现的高危HPV感染合并LSIL的患者182例行宫颈LEEP刀治疗的临床资料,观察治疗后的临床表现、宫颈创面的愈合情况、高危型HPV转阴率、宫颈液基细胞学的病理转归变化、切缘病变组织残留情况及术后并发症.结果 治疗后临床症状消失179例,好转3例,宫颈创面愈合总有效率100％;182例高危HPV感染合并LSIL的患者病理诊断慢性宫颈炎21例,CIN-Ⅰ 154例,CIN-Ⅱ 7例,治疗后3个月复查宫颈液基细胞学均阴性;高危HPV感染经LEEP刀治疗后HPV转阴153例,转阴率为84.1％;切缘病变组织无一例残留;治疗有效率为100％;并发症:术后阴道出血少量占180例,2例阴道出血较多,无一例发生宫颈管狭窄等其他并发症.结论 LEEP刀切除宫颈病变组织是治疗高危病毒HPV感染合并LSIL的安全有效方法.     

妊娠期宫颈机能不全围手术期处理因素对妊娠结局的影响

目的 探讨妊娠期宫颈机能不全围手术期处理因素对妊娠结局的影响.方法 回顾性分析我院收治的35例宫颈机能不全患者资料,均于妊娠中期采取McDonald法环扎宫颈,术后电话随访妊娠结局.根据妊娠结局分为流产组及活产组两组,分析围手术期处理因素对妊娠结局的影响.结果 流产9例,活产26例.流产组及活产组基本临床特征差异无统计学意义(P＞0.05).两组手术干预措施:手术缝线类型及手术时间对妊娠结局无影响(P＞0.05);采用黄体酮肌注者妊娠结局好于口服地屈孕酮者,差异有统计学意义(P＜0.05);活产组住院天数明显短于流产组,差异有统计学意义(P＜0.05).两组均未发生环扎线移位、胎膜早破、感染等并发症.结论 宫颈机能不全围手术期肌注黄体酮可提高活产率,改善妊娠结局.     

研究阴道镜在宫颈HPV感染诊断中的价值

目的探讨阴道镜对宫颈人乳头瘤病毒(HPV)感染的诊断价值。方法 120例行宫颈疾病检查患者,均行宫颈活组织病理学检查与阴道镜检查,并以宫颈活组织病理学检查结果作为金标准,观察分析阴道镜检查的准确度、敏感度、特异度、假阴性率与假阳性率。结果 120例患者宫颈活组织病理学检查结果显示:宫颈HPV亚临床感染(SPI)患者16例(13.3%), SPI伴子宫颈鳞状上皮内病变(SIL)患者2例(1.7%),宫颈炎患者77例(64.2%)。SPI阴道镜检查的准确度为93.75%(15/16),敏感度为93.75%(15/16),特异度为100.00%,假阴性率为6.25%(1/16),假阳性率为0(0/104);SPI伴SIL阴道镜检查的准确度为50.00%,敏感度为50.00%,特异度为100.00%,假阴性率为0,假阳性率为50.00%。结论阴道镜检查在宫颈HPV感染早期阶段、亚临床型与癌前病变诊断中应用价值较高。     

HPV分型检测在分流诊治ASCUS病例中的临床意义

目的探讨HPV检测在分流宫颈细胞学检查为ASCUS病例的临床意义及应用价值。方法 2011年7月至2012年7月,对211例细胞学检查为ASCUS的患者检测HPV感染类型,同时行阴道镜检查。结果①211例ASCUS中,108例为HPV阳性,其中高危型感染的102例。②阴道镜结果为宫颈炎症129例,宫颈湿疣及CINI55例,CINII-III26例,宫颈鳞癌1例。③高危型HPV阳性组中炎症35例、宫颈湿疣及CINI41例、CINII及以上病变26例,病变检出率为65.7%,CINII及以上病变检出率为25.5%。HPV阴性组中炎症、宫颈湿疣及CINI、CINII及以上病变分别为89例、13例、1例,病变检出率为13.8%,CINII及以上病变检出率为0.92%。二组之间病变检出率(χ2=58.192,P=0)及CINII及以上病变检出率差异具有统计学意义(χ2=26.942)。低危型HPV感染主要为炎症,仅发现1例低级别病变。利用高危型HPV检测ASCUS患者CIN及宫颈癌的阳性预测值为0.66,阴性预测值为0.86。④高危型HPV16、18感染对象和其他高危型HPV感染组在病变检出率上比较无统计学差异(χ2=2.995,P=0.084),二组之间CINII及以上病变检出率差异有统计学意义(χ2=12.237)。结论 HPV检测是分流ASCUS患者的有效方法,对高危型HPV感染的ASCUS患者,再进一步行阴道镜检查是较好的分流处理。高危型HPV感染的ASCUS患者HPV分型具有一定指导意义。     

高危型人乳头瘤病毒感染与宫颈癌前病变及宫颈癌的相关性分析

目的探讨高危型人乳头瘤病毒(HPV)感染与宫颈癌前病变以及宫颈癌的相关性。方法 120例宫颈癌或是癌前病变患者,其中60例为宫颈癌前病变患者(癌前病变组),60例为宫颈癌患者(宫颈癌组),将其归为观察组,同时期60例阴道炎宫颈无细胞学改变的患者设为对照组。采用宫颈液基细胞学检查法与宫颈刮片、免疫组化予以筛查分析,同时还采用达安PCR-反向点杂交法检测,对比分析各组检测结果。结果高危型HPV总阳性率为56.67%,癌前病变组、宫颈癌组以及对照组患者的高危型HPV阳性率分别为65%、95%、10%,三组患者的阳性率对比差异具有统计学意义(P<0.05)。结论高危型HPV感染与宫颈癌前病变以及宫颈癌具有非常密切的关系,而在高危型HPV感染中以HPV16感染为主。     

大庆油田27985例女工生殖健康体检结果分析

目的 通过高危型人乳头瘤病毒（HPV）检测等四项生殖健康检查,了解大庆油田女工生殖健康状况.方法 对2012年来本院体检的大庆油田27 985例女工的生殖健康体检资料进行回顾性分析.结果 参加体检人数27 985例,其中高危型HPV感染1080例,感染率为3.86％;子宫肌瘤和乳腺小叶增生的发病率分别为27.44％和14.93％；宫颈疾病的发病率为19.14％.结论 通过防癌普查,对筛查出的高危型HPV感染和宫颈疾病者建议进一步检查,早期发现宫颈癌前病变和早期宫颈癌,并及时阻断,从而降低宫颈癌的发生率和死亡率.     

保妇康凝胶治疗HPV感染的临床疗效分析

目的观察保妇康凝胶治疗宫颈人乳头瘤病毒（HPV）高危型感染的临床疗效。方法回顾性分析自2011年1月～2013年6月门诊收治的79例宫颈HPV高危型感染患者的临床资料,随机分为治疗组（62例）和对照组（17例）,治疗组予保妇康凝胶按用药说明进行治疗,对照组不用药,只随访观察。3个月后行HPV复查（治疗组停药一周）。结果治疗组44例（70．9％）HPV转阴,无不良反应发生,对照组中2例（11．7％）转阴,两组比较差异有统计学意义（P〈0．05）。结论保妇康凝胶能安全有效治疗宫颈高危型HPV感染。     

保妇康栓联合中药治疗宫颈HPV感染临床疗效观察

目的探讨保妇康栓联合中药治疗宫颈HPV感染的疗效。方法 2010年1月～2012年9月在我院采用病例对照研究的方法,将按宫颈细胞学检查TCT为炎症反应,排除宫颈病变,仅感染HPV高危亚型患者110例随机分为两组,观察组病例中,其中人乳头瘤病毒(HPV)高危亚型感染70例,应用保妇康栓联合中药,保妇康栓按用药说明进行治疗(每月月经干净后放保妇康栓,每晚一次,每次两粒),中药为汤药制剂,一天一剂,分3次服用;对照组40例,患者仅进行观察不做治疗。两组患者于用药3个月后结束,均进行HPV复查。结果应用保妇康栓及中药治疗3个疗程后,在保妇康栓及中药治疗组中,48例(68.6%)HPV高危感染转阴,在对照组中有13例(32.5%)HPV高危感染转阴。两组对照,宫颈HPV高危感染的有效性相比差异有统计学意义(P<0.01)。应用保妇康栓及中药治疗后,观察组中有48例(68.6%)HPV高危亚型转为阴性,而在对照组中只有13例(32.5%)HPV高危亚型转阴,两组相比差异具有统计学意义(P<0.01)。结论应用保妇康栓及中药治疗宫颈HPV高危感染是安全有效的,为临床处理提供了新思路。     

抗人绒毛膜促性腺激素β—亚基单克隆抗体杂交瘤的建立

用绒毛膜促性腺激素（HCG）及其β亚基（HCG-β）脾内免疫BALB/C小鼠后，取脾细胞与sp2/o小鼠骨髓瘤细胞融合，其融合剂PEG终浓度为37%，融合率100%，经反复筛选，克隆化后，获得三株持续分泌抗HCG-β单克隆抗体杂交瘤-E2、E12和F6，经亚型鉴定E2和E12为IgG1,F6为IgG2b。该杂交瘤所分泌的抗体公能与HCG和HCG-β特异性结合，而不与促共同体素（hLH)、促卵泡素（hFSH)和促甲状腺素（hTSH)发生交叉反应，并经ELISA抑制试验证实，单克隆抗HCG-β具有高度的特异性，可以为早孕和妇科肿瘤的特异性诊断提供原料，本文还对免疫程序各融合方法进行了讨论。     

PHARMACOLOGICAL COMPARISON OF HUMAN ISOLATED DIGITAL ARTERIES AND METACARPAL VEINS

1 The responses of human digital arteries and metacarpal veins obtained postmortem to various pharmacological agents have been tested. 2 pD₂ values for potassium chloride and barium chloride were found to be greater in arteries than in veins. 3 There was no difference between the arteries and veins in the pA₂ values for phentolamine as an antagonist of either noradrenaline or phenylephrine. pD₂ values for noradrenaline however, were significantly higher in the veins than in the arteries, whereas pD₂ values for phenylephrine in the two tissues were not significantly different. This raises the possibility of there being differences in the populations of a-adrenoceptors in the two tissues. 4 Differences were found between arteries and veins in the contractile and relaxant responses to histamine and in the antagonism of the responses to histamine by cimetidine and mepyramine, thereby suggesting differences in the populations of Hi-and H₂-receptors in these tissues. 5 No differences were found in the responses of arteries and veins to serotonin or in the antagonism of the response to this agonist by phentolamine. 6 Isoprenaline produced relaxant responses in veins (in which tone was induced with 30 mmol/1 potassium chloride) but not in arteries. 7 Dopamine produced very weak relaxant responses in preparations in which tone was induced using 30 mmol/1 potassium chloride. The mean £max value for this response was significantly greater in veins than in arteries. 8 Slight relaxant responses to acetylcholine were seen in veins and arteries precontracted with 30 mmol/1 potassium chloride. The mean £max value was significantly greater in veins than in arteries. 9. It is concluded that human digital arteries and metacarpal veins have differing pharmacological receptor populations and probably also differ in their non-receptor mediated contractile mechanisms.     

国产冻干人凝血酶原复合物质量标准的研究

目的通过对国产人凝血酶原复合物 (PCC)质量的研究 ,建立与国际接规的质量标准。方法用人凝血因子Ⅱ、Ⅶ、Ⅸ、Ⅹ (FⅡ、FⅦ、FⅨ、FⅩ )浓制剂国家标准品 ,采用一期法测定国内用不同原料制备的PCC中FⅡ、FⅦ、FⅨ、FⅩ的效价 ;用正常人血浆作标准 ,用凝固法检测PCC的总效价及PCC中的肝素。结果用血浆作原料提纯制备的PCC ,FⅣ的效价能达到 1 0IU/ml以上 ,FⅦ效价最低 ;用组分Ⅲ作原料 ,FⅣ活性损失很大 ,只有 2 / 7批次能达到 1 0IU/ml;而FⅦ效价最高。无论用何种原料 ,FⅡ和FⅩ活性均能保持在一定水平。结论从血浆中直接提纯制备PCC ,能有效地保持FⅣ活性 ,各项指标易达到国外同品种的质量标准     

论人体生命的三重品格

本文讨论的是人体生命观的问题,从人的本质来看,人体生命与其它生命体有着原则的区别。人体生命是神圣品格、质量品格和价值品格的辩证统一。人体生命三重品格以价值品格为核心,密不可分地联系在一起。片面强调人体生命的质量品格或神圣品格,必然使人们在理论和实践上陷入二难境地。     

Human embryonic stem cells and lung regeneration

Human embryonic stem cells are pluripotent cells derived from the inner cell mass of preimplantation stage embryos. Their unique potential to give rise to all differentiated cell types has generated great interest in stem cell research and the potential that it may have in developmental biology, medicine and pharmacology. The main focus of stem cell research has been on cell therapy for pathological conditions with no current methods of treatment, such as neurodegenerative diseases, cardiac pathology, retinal dysfunction and lung and liver disease. The overall aim is to develop methods of application either of pure cell populations or of whole tissue parts to the diseased organ under investigation. In the field of pulmonary research, studies using human embryonic stem cells have succeeded in generating enriched cultures of type II pneumocytes in vitro. On account of their potential of indefinite proliferation in vitro, embryonic stem cells could be a source of an unlimited supply of cells available for transplantation and for use in gene therapy. Uncovering the ability to generate such cell types will expand our understanding of biological processes to such a degree that disease understanding and management could change dramatically.     

Human Herpesvirus 6 (HHV-6) Induces Dysregulation of Glutamate Uptake and Transporter Expression in Astrocytes

Human herpesvirus 6 (HHV-6) infects and establishes latency in the central nervous system (CNS). Reactivation of latent HHV-6 has been associated with neurologic diseases including epilepsy and multiple sclerosis (MS). In vivo, HHV-6 has been localized to astrocytes and can infect human astrocytes in vitro, suggesting that this virus may have a tropism for glial cells and may affect glial cell function. An essential role of astrocytes in the CNS is active maintenance of the excitatory neurotransmitter glutamate. Dysregulation of glutamate has been implicated as a potential mechanism of disease in both epilepsy and MS. Both disorders have demonstrated elevated glutamate in CSF and may be associated with dysregulation of glutamate signaling, uptake, and metabolism. This study demonstrates dysregulation of glutamate uptake in human astrocytes infected with both variants of HHV-6, A and B, with differential effects of HHV-6 in acute and persistently infected cells. Whereas astrocytes acutely infected with HHV-6 demonstrated increased glutamate uptake, cells persistently infected with HHV-6A and HHV-6B demonstrated impaired glutamate uptake. Functional dysregulation of glutamate uptake was associated with early increases in mRNA and protein expression of the glial glutamate transporter EAAT-2 followed by a sustained decrease in mRNA expression in astrocytes infected with both HHV-6A and HHV-6B. Dysregulated glutamate uptake and transporter expression suggests a mechanism for dysregulation of glutamate levels in vivo and a potential mechanism for virus-associated neurologic disease. This work was supported by the National Institutes of Health/National Institute of Neurological Disorders and Stroke. JF was supported by the MS Society of Canada.     

Resolution of human mercapt- and nonmercaptalbumin by high-performance liquid chromatography

Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the second one to human nonmercaptalbumin (HNA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak on PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.     

Carbonic Anhydrase Inhibition with Benzenesulfonamides and Tetrafluorobenzenesulfonamides Obtained via Click Chemistry

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Further studies on the resolution of human mercapt- and nonmercaptalbumin and on human serum albumin in the elderly by high-performance liquid chromatography

High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on Asahipak GS-520 showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary one to nonmercaptalbumin (HNA). HPLC analysis of HSA on Asahipak ES-520 N showed three peaks, the principal component corresponding to HMA, the secondary one to HNA having mixed disulfide with cysteine or glutathione and the tertiary one to HNA oxidized higher than mixed disulfide. Two kinds of rapid HPLC for the resolution of HSA into HMA and HNA were developed by the present authors. Using these HPLC, the present authors found a significant decrease in the fraction of HMA in the elderly.     

首批人凝血因子Ⅱ和Ⅹ浓制剂国家标准品协作标定

目的协作标定首批人凝血因子Ⅱ和Ⅹ浓制剂国家标准品。方法用世界卫生组织 98 590批人凝血因子Ⅱ和Ⅹ浓制剂国际标准品 ,采用一期法标定我国首批人凝血因子Ⅱ和Ⅹ浓制剂国家标准品 ,将标准品分别于4℃、2 2℃、37℃保存 5个月 ,进行稳定性考查。结果人凝血因子Ⅱ和Ⅹ国家标准品的效价分别为 1 6 .1IU 支和 1 2 .1IU 支 ,稳定性良好。结论确定了首批人凝血因子Ⅱ和Ⅹ浓制剂的国家标准品