非小细胞肺癌患者外周血单个核细胞Th型细胞因子分泌

细胞免疫分为Ｔｈ1型和Ｔｈ2型[1,2 ] 。Ｔｈ1型细胞能通过分泌白介素 2 (ＩＬ 2 )、干扰素γ(ＩＦＮ γ)等细胞因子促进细胞免疫 ;而Ｔｈ2型免疫却通过白介素 10 (ＩＬ 10 )、白介素 4 (ＩＬ 4 )等细胞因子抑制Ｔｈ1型细胞免疫。肿瘤患者存在Ｔｈ免疫功能的紊乱[3 ] 。我们的实验以非小细胞肺癌 (ＮＳＣＬＣ)患者为研究对象 ,检测了外周血单个核细胞 (ＰＢＭＣ)分泌的细胞因子水平 ,发现ＮＳＣＬＣ患者存在Ｔｈ型细胞免疫失衡。对象与方法　研究对象 :30例ＮＳＣＬＣ患者 ,年龄 36～ 70岁 ,平均年龄 5 6岁 ,男 2 1例 ,女 9例 ,鳞癌 18例 ,…     

慢性乙型肝炎患者外周血单个核细胞Th1、Th2型细胞标记物及其细胞因子的表达

Ｔｈ1/Ｔｈ2 细胞在宿主抵御病毒感染中的作用日益受到重视 ,Ｔｈ1/Ｔｈ2 免疫应答与乙型肝炎的关系也是近年研究的热点。但由于缺乏特异性高且操作简便的Ｔｈ1、Ｔｈ2 细胞标记物 ,这方面的研究仅限于测定其所代表的细胞因子含量。由于细胞因子是以网络形式存在 ,影响其分泌的因素很多 ,所以仅从Ｔｈ分泌的细胞因子来分析Ｔｈ1/Ｔｈ2 功能有一定的局限性。近来发现 ,ＣＣＲ5、ＣＤ30分别为Ｔｈ1、Ｔｈ2细胞特异表达 ,且与激活Ｔｈ1、Ｔｈ2 密切相关 ,因此可作为其相应的表面标记物[1,2 ] 。为了阐明Ｔｈ1、Ｔｈ2 细胞在慢性乙型肝炎中的作…     

子宫肌腺症患者外周血单个核细胞T辅助细胞因子水平检测及意义

子宫肌腺症是指子宫内膜在子宫肌层良性浸润并在其中弥漫性生长 ,既往认为本病发生与刮宫造成子宫内膜基底层损伤有关 ,但其很难解释年轻未育妇女为何也会发病。近年来有研究表明 ,免疫机制可能参与了子宫肌腺症的发病。我们采用外周血单个核细胞 (PBMC)加特异性刺激 T细胞增殖的丝裂原植物血凝素 (PHA)体外诱生培养 ,用酶联免疫吸附(EL ISA)技术测定了 32例本病患者 PBMC中 T辅助细胞 1(Th1)、类细胞因子干扰素 (IFN ) -γ、Th2类因子白细胞介素(IL ) - 4及 IL - 10的含量 ,旨在探讨 Th细胞亚群在子宫肌腺症发病中的作用。资料…     

丙型肝炎患者外周血单个核细胞对特异性HCV抗原增殖反应

丙型肝炎病毒（ＨＣＶ）的发病机制和机体对ＨＣＶ的免疫应答目前尚不十分清楚。为此,我们研究了丙型肝炎（ＨＣ）患者外周血单个核细胞（ＰＢＭＣ）对ＨＣＶ特异性抗原的增殖反应,从细胞免疫的角度分析机体抗ＨＣＶ免疫功能及其与临床预后的相关性。材料与方法一、病例...     

炎症性肠病患者外周血单个核细胞细胞毒性 T淋巴细胞相关抗原4的表达

背景:细胞毒性T淋巴细胞相关抗原(CTLA)4是一种重要的免疫细胞共刺激分子,主要表达于活化的T淋巴细胞,对T细胞的激活起抑制作用。目的:通过检测炎症性肠病(IBD)患者外周血单个核细胞表面CTLA4的表达,探讨CTLA4在IBD发病中的作用和功能。方法:将18例活动性溃疡性结肠炎(UC)、4例活动性克罗恩病(CD)患者以及21名健康对照者外周血单个核细胞分离后,以流式细胞仪检测细胞表面CTLA4、CD4 和CD8 的表达。结果:自然状态下,UC和CD患者CD4 CTLA4 表达显著高于对照组(8.2%±4.7%对1.4%±1.3%,P<0.001;4.3%±2.9%对1.4%±1.3%,P=0.011);CD8 CTLA4 表达亦显著高于对照组(9.1%±7.2%对0.9%±0.3%;8.7%±3.5%对0.9%±0.3%,P均<0.001)。经植物血凝素(PHA)刺激后,IBD患者和对照组外周血单个核细胞CTLA4的表达均增加;CD组CD8 CTLA4 表达显著高于对照组(35.0%±10.9%对18.1%±10.1%,P=0.005)。结论:活动性UC和CD患者外周血单个核细胞CTLA4表达增强,提示T细胞激活第二信号通路B7/CD28/CTLA4在IBD的发病过程中起重要作用。     

氯喹抑制LPS和CpG DNA诱导人外周血单个核细胞释放促炎细胞因子的研究

目的 观察氯喹对脂多糖(LPS)和CpG DNA诱导人外周血单个核细胞释放促炎细胞因子的抑制作用。方法 采用密度梯度离心法从新鲜人外周血中分离单个核细胞(hPBMC)。先用LPS和CpG DNA刺激hPBMC分泌TNF-α、IL-6,再加入不同浓度氯喹进行拮抗,观察其量效关系;在不同时相点加入氯喹观察时效关系;用ELISA法检测培养上清中促炎细胞因子的含量。采用甲基四唑蓝(MTT)法检测氯喹对细胞活性的影响。结果 氯喹在50μg/ml时显著抑制LPS和CpG DNA刺激hPBMC释放TNF-α和IL-6(P<0.01)。若先给氯喹,则其拮抗细胞因子释放的作用非常显著(P<0.01),提前给予LPS或CpG DNA,再给氯喹时也可观察到显著拮抗作用(P<0.05)。氯喹浓度小于100μg/ml时对细胞活性无显著影响(P>0.05)。结论 氯喹对LPS和CpGDNA诱导人外周血hPBMC释放促炎细胞因子的抑制作用呈明显的剂量和时间依赖关系。     

重症肌无力患者外周血单个核细胞中糖皮质激素受体不同亚型表达和意义

目的探讨重症肌无力（MG）患者外周血单个核细胞（PBMC）中两种糖皮质激素受体（GR）亚型表达水平及其与糖皮质激素疗效的关系。方法在糖皮质激素治疗之前留取MG患者PBMC并涂片,根据糖皮质激素治疗MG的不同疗效分组,通过免疫细胞化学方法观察各组GRα和GRβ阳性细胞比例并评分,分析GRα和GRβ表达量与糖皮质激素疗效的关系。结果对照组、糖皮质激素治疗敏感组和依赖组PBMC中GRα阳性细胞计分差异无统计学意义,3组计分明显高于糖皮质激素治疗抵抗组（P〈0．01）;对照组、糖皮质激素治疗敏感组和糖皮质激素治疗抵抗组PBMC中GRβ计分差异无统计学意义,3组计分明显低于糖皮质激素治疗依赖组（P〈0．01）。结论MG患者PBMC中GRα表达降低和GRβ表达增高均与糖皮质激素疗效降低有关。     

IL-12对干扰素α治疗的慢性乙型肝炎患者外周血单个核细胞产生细胞因子的影响

目的探讨干扰素α治疗慢性乙型肝炎过程中IL-12对其外周血单个核细胞(PBMC)产生IFNγ和IL-10的动态影响. 方法用ELISA法分别检测20例慢性乙型肝炎患者在干扰素α2b治疗前、治疗3个月、治疗6个月时PBMC在PHA(100μg/ml)、HBcAg(1μg/ml)、HBeAg(1μg/ml)单独或联合IL-12(10ng/ml)体外培养48h后培养上清液INFγ和IL-10的水平. 结果 12例干扰素应答患者联合IL-12诱导,同一诱导组在治疗3个月和6个月同治疗前比较,IFNγ水平明显增高(P＜0.01),同一治疗阶段,单独抗原诱导和联合IL-12诱导相比,联合诱导组IFNγ水平明显增高(P＜0.01),治疗3个月和6个月时,IL-12对IL-10的产生表现出明显的抑制作用(HBcAg诱导组例外).8例干扰素无应答患者联合IL-12诱导,同一诱导组在治疗3个月和6个月同治疗前相比,IFNγ水平明显增高(P＜0.01),同一治疗阶段,单独抗原诱导和联合IL-12诱导相比,联合诱导组IFNγ水平明显增高,并随治疗时间的延长,协同效应更为明显. 结论在干扰素治疗过程中,IL-12对乙型肝炎患者PBMC产生IFNγ表现明显的协同效应,特别对无应答患者产生IFNγ协同效应时间明显缩短,提示IL-12 联合干扰素α可能提高干扰素α治疗慢性乙型肝炎的疗效.     

系统性红斑狼疮外周血单个核细胞生长激素受体和mRNA表达的研究

目的　探讨SLE患者外周血单个核细胞 (PBMC)的生长激素受体 (GHR)的表达与系统性红斑狼疮 (SLE)的关系。方法　采用放射性受体配基结合试验 (RLBA)及反转录 聚合酶链反应(RT PCR)研究外周血淋巴细胞GHR变化。结果　SLE活动期患者PBMC的GHR的特异性结合率 (SB) (3 4± 2 0 ) %、总结合率 (TB) (13 8± 5 7) %和静止期患者SB (1 3± 1 2 ) % ,TB (11 4±4 6 ) %均高于正常对照组SB (1 0± 1 0 ) % ,TB (8 3± 0 9) % (P <0 0 1) ;SLE活动期患者PBMC的mRNA的表达水平 (0 77± 0 6 6 )高于静止期患者 (0 4 5± 0 2 8) (P <0 0 5 )和正常对照组PBMC的mRNA的表达 (0 31± 0 2 2 ) (P <0 0 1)。结论　SLE患者PBMC的GHR高表达与SLE的病情活动性相关。     

Inhibition of tissue factor surface expression in human peripheral blood monocytes exposed to cytokines

Interleukin (IL)-4, IL-10, IL-13 and transforming growth factor beta (TGF-β) are known to regulate several monocyte functions, including inhibition of the synthesis of different cytokines. Using quantitative RT-PCR and flow cytometry analysis we investigated the effects of these cytokines on bacterial lipopolysaccharide (LPS)-induced tissue factor (TF) expression in human monocytes. The effects of IL-4 and IL-10 on monocyte chemoattractant protein-1 (MCP-1)- and C-reactive protein (CRP)-induced TF expression were also studied. A direct comparison revealed that IL-4, IL-10 and IL-13 all down-regulated LPS-induced TF expression in a concentration-dependent manner without the need for priming. In contrast, TGF-β required 4 h of priming to inhibit TF expression induced by LPS. IL-10 was the most powerful inhibitor, causing almost complete inhibition at 5 ng/ml. IL-4 and IL-13 exhibited a significantly lower inhibitory capacity even at concentrations of 100 ng/ml. IL-4 and IL-10 showed similar concentration-dependent inhibition of MCP-1- and CRP-induced TF expression. We also showed that the regulatory effect of the interleukins occurred at the mRNA level. In vivo , these inhibitory cytokines may play an important regulatory role in preventing thrombosis. IL-10, in particular, may be a possible candidate as a TF-preventing drug.     

肺组织细胞凋亡与血清细胞因子变化的关系

目的 探讨大鼠肾缺血再灌注后肺组织细胞凋亡及血清细胞因子的的变化,分析其在肾缺血再灌注后肺损伤发病机制中的作用.方法 用无损伤动脉夹钳夹大鼠双侧肾蒂45 min制成肾缺血再灌注损伤模型.观察缺血再灌注后2 h、6 h、12 h、24 h、72 h血清白介素1β(IL-1β)和一氧化氮(NO)的浓度,病理切片观察肺组织病...     

Kinetics of serum cytokines in adults undergoing peripheral blood progenitor cell transplantation

Summary. We investigated the serum cytokine levels (G-CSF, GM-CSF, IL-l/?, IL-3 and IL-6) using an ELISA in 14 patients with haematological malignancies undergoing peripheral blood progenitor cell transplantation (PBPCT). Serum G-CSF levels in all patients rose immediately after PBPCT, then gradually decreased as the neutrophil counts began to rise. No detectable serum levels of GM-CSF or IL-lp were observed, but serum levels of IL-3 rose transiently immediately following PBPCT. Serum levels of JL-6 rose transiently during a fever in four patients. These observations suggest that G-CSF and L 3 may contribute to the early haemopoietic reconstitution in PBPCT.     

新疆乌鲁木齐地区人群碘摄人量与甲状腺疾病的相关研究

目的 了解乌鲁木齐地区人群目前碘营养状况、甲状腺疾病流行情况并探讨两者的关系.方法 对新疆乌鲁木齐地区1693名成人进行体格检查、问卷调查,并测定平均尿碘中位数、甲状腺功能、甲状腺相关抗体和进行甲状腺B超检查.结果 在调查人群中,碘缺乏人数仅占9.5％,而碘过量人数达到30.0％.平均尿碘中位数达254.9 μg/L.碘充足/超足量组TT4水平明显高于其他两组[(9.02±2.63)对(7.69±2.85)、(8.45±2.13)μg/dl,均P＜0.05],碘缺乏组TSH水平显著高于其他两组[(3.00±1.86)对(2.37±1.91)、(2.27±1.86) mIU/L,均P＜0.01].各组甲状腺球蛋白抗体、甲状腺过氧化物酶抗体阳性率及甲状腺功能异常、甲状腺结节患病率没有统计学差别.结论 目前新疆乌鲁木齐地区碘缺乏状态已纠正,平均尿碘中位数254.9 μg/L.不同碘摄入状态人群甲状腺疾病患病率无统计学差别.     

Membrane expression and synthesis of p23,30 (HLA-DR antigen) by human peripheral blood monocytes

The synthesis and expression of protein complexes of 23,000 and 30,00 dalton (p23,30) HLA-DR antigen, and of 44,000 and 12,000 dalton (p44,12) HLA-A,B antigen and beta 2-microglobulin was demonstrated on human peripheral blood monocytes and in cultures of purified, adherent monocytes. In indirect immunofluorescence assays, a gradation in the intensity of staining with rabbit anti-p23,30 serum was present but clearly p23,30-negative, actively phagocytic monocytes were not found. In contrast the fluorescence intensity of staining with a serum to p44,12 (HLA-A,B antigens and beta 2-microglobulin) was constant on all macrophages. Macrophage HLA-DR antigen was shown in SDS gels of immunoprecipitates of 35S-methionine-labeled, detergent-solubilized membrane proteins to be composed of 29,000 and 34,000 dalton, noncovalently linked chains, which form was indistinguishable from that of B lymphoblastoid cell line HLA-DR antigens. The rate of synthesis of HLA-DR antigen was about 17% of that of synthesis of HLA-A,B antigens in adherent macrophage populations. The variable expression of p23,30 on peripheral blood monocytes was consistent with the view of the existence of subsets of such monocyte populations. These findings were compared to studies by others of the variable expression of Ia antigens on human, murine and guinea pig monocytes populations.     

急性心肌梗死患者外周血T淋巴细胞及其亚群与C-反应蛋白的相关性

目的:研究急性心肌梗死(AMI)患者外周血T淋巴细胞及其亚群和血清C反应蛋白(CRP)的变化,探讨两者之间的相关性。方法:采用流式细胞仪技术测定33例AMI患者外周血T淋巴细胞及其亚群,并与对照组的30例比较;采用免疫散射比浊法测定33例AMI患者血清CRP的系列变化值,取其峰值。结果:AMI患者外周血CD3+和CD4+显著低于对照组(P<0.01),CD8+无明显改变(P>0.05),CD4+/CD8+比值明显低于对照组(P<0.01);CRP峰值与CD4+/CD8+比值呈显著的负相关(r=-0.731,P<0.01)。结论:AMI患者细胞免疫功能受到抑制,T细胞亚群的变化与炎症因子CRP有相关性,炎症因子及免疫机制可能都是影响AMI患者病情进展的重要因素。     

淋巴细胞亚群和细胞因子在Graves病患者外周血中的漂移特征

目的观察分析外周血淋巴细胞亚群及细胞因子的变化在Graves病（GD）发生发展中的临床意义。方法分别检测GDa组（初诊）、GDb组（治疗后）、GDe组（复发）和正常组外周血中淋巴细胞亚群、IL-12、IL-10的表达。结果与对照组相比,GDa组和GDc组CD^＋8、CD^＋3及CD^＋16淋巴细胞显著下降（P〈0.01）,CD^＋4／CD^＋8比值和CD^＋19;明显升高（P〈0.01）;GDa组的IL-10、IL-12均高于对照组（P〈0.05）;GDb组的IL-12和IL-12／IL-10较GDa组显著降低（P〈0.05）;GDa和GDc组的CD^＋16与CD^＋8呈明显正相关（P〈0.05）,与CD^＋19呈明显负相关（P〈0.01）。结论GD患者淋巴细胞亚群的分布特征和Thl／Th2的平衡紊乱在GD的发生发展过程中有着重要临床意义。     

Associations between elevated pre‐treatment serum cytokines and peripheral blood cellular markers of immunosuppression in patients with lymphoma

Higher ratios of the pre‐treatment peripheral blood absolute lymphocyte (ALC) to absolute monocyte counts (AMC) are associated with improved outcomes in lymphoma. Conversely, elevated pre‐treatment serum cytokines are associated with inferior outcomes. The relationship between pre‐treatment serum cytokines and ALC/AMC ratios remains unknown. We studied twelve serum cytokines and the ALC/AMC ratios in 390 patients with untreated diffuse large B‐cell, follicular, mantle cell, T‐cell, and Hodgkin lymphoma. Different pre‐treatment serum cytokine concentrations correlated with ALC, AMC, and ALC/AMC ratios depending on the lymphoma type. In the entire cohort (n = 390) lower ALC/AMC ratios modestly correlated with higher IL‐2R (r = ?0.36), IL‐12 (r = ?0.17), IP‐10 (r = ?0.23), and MIG (r = ?0.32) concentrations (p < 0.001). Elevated IL‐2R was independently associated with suppressed ALC (OR 2.69, 95% CI 1.77–4.07, p < 0.001), elevated AMC (OR 2.05, 95% CI 1.34–3.14, p < 0.001), and suppressed ALC/AMC ratios (OR 3.51, 95% CI 2.31–5.34, p < 0.001). Both elevated IL‐2R (HR 2.27, 95% CI 1.48–3.49, p < 0.001) and suppressed ALC/AMC ratios (HR 1.53, 95% CI 1.03–2.28, p = 0.037) were independently associated with inferior overall survival. These data support the notion that elevated serum cytokines are immunosuppressive and provide further rationale to target the tumor microenvironment for therapeutic benefit.