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Objective: To observe the influence of human mutant p27 gene (p27mt) on the growth and apoptosis of colon cancer cells so as to investigate the function mechanism of p27mt in gene therapy for colon cancer. Methods: Colon cancer cell line SW480 was infected with recombinant replication defective adenovirus Ad-p27mt, and expression of p27mt protein was detected by Western blot; the inhibition effect of p27mt on SW480 cells was detected with cytometry. Cell cycle was decided with flow cytometer, and DNA fragment analytic process identified the occurrence of apoptosis. Results: After transfected SW480 cells with Ad-p27mt, high expression of p27 protein was identified with immunoblotting assay. PI staining and flow cytometer assay showed 77.96% colon cancer cells was blocked in phase G0/G1, while in Ad-LacZ group and blank control group, 27.57% and 25.29% cells were blocked in the same phase, respectively. Growth curve showed Ad-p27mt has an obvious inhibition effect on the growth of SW480 cells, DNA fragment assay demonstrated that p27mt was able to induce the apoptosis of colon cancer cells. Conclusion: p27mt has an obvious blocking effect on colon cancer cell cycle, and most cells were blocked in phase G0/G1. This blockage is related with the growth inhibition and apoptosis induction effect of p27mt. 相似文献
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Objective: To research the contributions of p130Cas and PTEN signal molecules to the carcinogenesis of gastric carcinoma and the relationship between them. Methods: Detecting proteins of p130Cas, PTEN and PTEN mRNA of 76 cases normal gastric mucosa and 112 cases gastric carcinoma by immunohistochemistry EnVision method and molecular hybridization in situ method respectively. Detecting PTEN genetic mutation of 30 cases normal gastric mucosa, 7 cases early gastric cancer and 30 cases progressive gastric cancer by PCR-SSCP. Results: The expression of p130Cas protein of gastric carcinoma increased significantly than that of normal gastric mucosa (P 〈 0.05). Opposite to above, the expression of PTEN protein of gastric carcinoma group was significantly lower than that of normal gastric rnucosa group (P 〈 0.05). The expression of PTEN mRNA of gastric carcinoma group decreased obviously than normal gastric mucosa group (P 〈 0.001). Only one case exon 5 and one case exon 8 of PTEN appeared gene mutation of progressive gastric carcinoma group, the difference has no significance compared with normal gastric mucosa group and early gastric cancer group. Conclusion: The signaling molecules p130Cas and PTEN play an important role in the carcinogenesis of gastric carcinoma, and p130Cas plays the part of promoter, oppositely, maybe PTEN can inhibit it. 相似文献
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Chenxi Yin Chang Jiang Fangxin Liao Yuming Rong Wenzhuo He Xiuyu Cai Guifang Guo Huijuan Qiu Xuxian Chen Bei Zhang Liangping Xia 《德国医学》2014,(4):169-173
Objective: We aimed to evaluate the effect of bevacizumab in the palliative treatment of Chinese metastatic colorectal cancer (mCRC) and its efficacy in different lines. Methods: Patients of mCRC treated with bevacizumab or not at Sun Yat-sen University Cancer Center from 2005 to 2013 were recruited as the study group and control group. The endpoints were objective response rate (ORR), disease control rate (DCR), overall survival (OS) and progression free survival (PFS). The OS and PFS of first-, second- and third-line treatment groups were compared between study group and control group. Re- sults: The median PFS of the study and the control group were 8.2 months (7.0-9.4 months), 5.7 months (4.7-6.6 months), P = 0.001; OS were 26 months (5.4-130.5 months), 18 months (16.6-19.4 months), P 〈 0.001, respectively. The ORR and DCR of first-, second- and third-line were 30.3% (20/66), 20% (6/30), 17.6% (3/17) and 97% (64/66), 86.7% (26/30), 100% (17/17). In the first-line chemotherapy group, the OS of the study group and the control group were 22.9 (5.4-96.7) months and 18 (16.6-19.4) months (P 〈 0.001); PFS were 9.4 (8.4-10.4) months and 5.7 (4.7-6.6) months (P 〈 0.001), respectively. While in the second- and third-line setting, only OS were statistically different, PFS had no significant difference. Conclusion: The combination of bevacizumab and chemotherapy had a promising short-term and long-term efficacy in Chinese mCRC patients than those without bevacizumab regimens, and the effect could be better reflected in the first-line treatment. 相似文献
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Liangping Xia Guifang Guo Huijuan Oiu Bei Zhang Feifei Zhou 《德国医学》2009,(9):526-530
Objective: The aim of this study was to investigate the efficacy and safety profiles of cetuximab, the commonly used monoclonal antibody of epidermal growth factor receptor, based on Chinese patients with colorectal cancer. Methods: All the papers studied on Chinese patients with gastrointestinal cancer treated by cetuximab and found in both databases of Chinese journal database for fulltext and PubMed were collected. The commonly used efficacy index such as disease control rate (CR + PR + SD) and response rate (CR + PR) were analyzed, and the cetuximab related side effects such acne-like rash and nail change and hypersensitivity were analyzed too. Results: (1) There were 10 original papers contained total 152 patients with gastrointestinal cancer who were the candidates to analyze the safety profiles, and contained 130 patients with colorectal cancer who were candidates to analyze the efficacy. (2) The disease control rate and response rate in the whole group was 73.5% (95% CI: 65.5%-81.5%) and 29.1% (95% CI: 20.9%-37.3%), respectively; In first-line setting group they were 70.0% (95% CI: 55%-85%) and 41.7% (95% CI: 25.6%-57.8%), respectively; In non-first line setting group they were 71.6% (95% CI: 61.8%-81.4%) and 23.5% (95% CI: 14.3%-32.7%), respectively. The disease control rate between the two line-setting groups was insignificant, but the response rate between the two line-setting groups was significant (P = 0.045). (3) The incidence of acne-like rash was 72.0% (95% CI: 64.8%-79.2%), the degree Ⅰ-Ⅱ and degree Ⅲ-Ⅳ account for 56.0% (95% CI: 48.1%-63.9%) and 16.0% (95% CI: 10.1%-21.9%), respectively. No treatment related death, and the hypersensitivity was under control. Conclusion: This study is the first study to summarize the data of Chinese patients with colorectal cancer treated by cetuximab-contained regimen, it showed that the monoclonal antibody was effective and safe for Chinese patients as the West p 相似文献
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Xiaofang Liu ;Yongliang Duan ;Fanmin Kong ;Zheng Xu ;Xianting Zhou ;Cuisheng Zhang ;Shaojun Li 《德国医学》2009,(4):214-218
Objective: To study the methylation status of several genes on p53-Bax mitochondrial apoptosis pathway and clinical significance in cholangiocarcinoma. Methods: Promoter hypermethylation of DAPK, p14 and ASC genes were detected by methylation-specific PCR. p53 gene status (exon 5-8) were examined by automated sequencing, combined with the clinical documents of patients by statistics analysis. Results: (1) We found 66.7% of 36 cases cholangiocarcinoma had methylation of at least one tumor suppressor gene. The frequency of tumor suppressor gene methylation in cholangiocarcinoma was: p14 (24%), DAPK (30.6%), TMSI/ASC (36.1%). The frequency of tumor suppressor gene methylation in tissues near cancer was: DAPK (5.6%), TMS1/ASC (8.3%). (2) p53 gene mutations were found in 22 of 36 patients (61.1%). (3) There were no statistically relationship among the methylation of DAPK, p14 and ASC genes. There were negative relationship differences between the methylation of p14 and p53 gene mutation (P 〈 0.05). (4) p53 gene mutation combined with the methylation of tumor suppressor were 14 cases (38.9%). There were statistically differences on extent of pathologic biology, differentiation and invasion (P 〈 0.05). Conclusion: Our study indicated that methylation of p53-Bax mitochondrial apoptosis pathway in cholangiocarcinoma was a common epigenetic event. Although the methylation of ASC, DAPK genes was low, it might be significance for early diagnosis, p53 gene mutation combined with the methylation of tumor suppressor might be relationship with pathologic biology, it trended to more malignancy. 相似文献
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Objective: The aim of this study was to discuss HIF-la expression and vasculogenic mimicry (VM) in hepatocel- lular carcinoma (HCC) and their relationship with the clinical pathological features and clinical significance. Methods: Two hundred and seven specimens from patients in The Affiliated Hospital of North Sichuan Medical College who received hepatic cell carcinoma resection were tested by immunohistochemistry and double staining of CD31 and PSA. Then detected the expression of HIF-la, VM, and analysed the relationship between clinical pathology. Results: The HIF-la positive rate was 71.01% and its expression was associated with liver cirrhosis, tumor size and TNM stage (P 〈 0.05). HIF-la protein expres- sion was positively associated with the VM (y = 0.1988, P = 0.0041). Conclusion: Hypoxia may be the reason for VM in high invasive HCC, regulating the tumor microenvironment may have great significance in inhibiting invasion and metastasis of HCC. 相似文献
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Shenqiu Li Ojnjing Wei 《德国医学》2009,(5):286-288
Objective: To study the expression of eukaryotic initiation factor-4E (eIF-4E) and p53 in squamous cell carcinomas (SCC) and to explore their relationship and clinical significance. Methods: The expression of elF-4E and p53 in 32 cases of SCC was detected by immunohistochemical SABC method. Results: The positive rate of elF-4E and p53 expression was 93.8% and 56.3% in SCC, and the levels of eIF-4E and p53 were significantly higher in SCC than those in the normal skin tissue (P 〈 0.05). Conclusion: Both elF-4E and p53 were useful markers in SCC, but the specialty and sensitivity of the eiF- 4E protein was high in SCC. 相似文献