食管鳞癌组织中JWA、FAK表达变化及其与淋巴结转移的关系

目的观察食管鳞癌组织中JWA蛋白和黏着斑激酶（FAK）的表达变化,并探讨其与食管鳞癌淋巴结转移的关系。方法采用蛋白免疫印迹法检测37例食管鳞癌患者肿瘤组织及其癌旁组织中的JWA、FAK蛋白。结果食管鳞癌组织与癌旁组织中JWA蛋白的相对表达量分别为0.302±0.119、0.638±0.128,FAK分别为0.717±0.153、0.432±0.097,二者JWA、FAK蛋白表达量相比,P均〈0.05。有、无淋巴结转移者JWA蛋白相对表达量分别为0.163±0.37、0.383±0.06,FAK蛋白分别为0.832±0.134、0.658±0.109,P均〈0.05。JWA、FAK蛋白在食管鳞癌组织中的表达呈负相关（r=-0.736,P〈0.01）。结论食管鳞癌组织中JWA蛋白表达下调,FAK蛋白表达上调,且其表达变化与食管鳞癌淋巴结转移有关。JWA可能通过抑制FAK的表达,发挥抑制食管鳞癌淋巴结转移的作用。     

Expressions of p53, VEGF C,p21: could they be used in preoperative evaluation of lymph node metastasis of esophageal squamous cell carcinoma?

The prognosis of esophageal squamous cell carcinoma is primarily determined by staging. Although radiological methods have revealed lymph node metastasis preoperatively, these radiological findings cannot be correlated with pathological staging. The aim of this study was to compare the expressions of p53, vascular endothelial growth factor C (VEGF C) and p21 with lymph node metastasis in preoperative endoscopic biopsy and postoperative resection material. Tissue samples were taken from 40 patients who had undergone endoscopic biopsies and radical esophagectomies. The expressions of p53, VEGF C and p21 proteins in these sections were immunohistochemically examined. The expression of each antibody was characterized as a negative or positive reaction according to the pattern and intensity of semiquantitative immunostaining. The staining pattern of antibodies was divided into three groups: < 10% cancer cells were accepted to be (-), 10-50% were (+), heterogenous and > 50% were (+ +), homogenous. For each antibody, statistical correlation with conventional prognostic parameters such as localization, microscopic grade, stage, pathological lymph node metastasis and survival, were investigated. p53 expression was observed in 65.5% (19/29) of lymph node positive cases, whereas p53 was in 50% (20/40) of cases. VEGF C was in 65% (26/40) and p21 was in 15% (6/40) of cases. p53 has the specificity of 90.9% and sensitivity rate of 65.5% in detecting lymph node metastasis and positive predictive value was 95%. Expression of p53 was significantly correlated with stage and lymph node metastasis (P = 0.02 and P = 0.03, respectively). Prediction of lymph node metastasis by p53 was correlated independently and in coexpression with VEGF C (P < 0.01). There was no relation detected between p21 and other parameters. In esophageal squamous cell carcinoma (SCC), p53 and VEGF C expressions were correlated with pathologically positive lymph nodes. When preoperative staging has been insufficient in esophageal carcinoma cases, immunohistochemical analysis of p53 and VEGF C staining in tissues could be an aid to clinicians regarding lymph node metastasis.     

食管鳞癌中基质金属蛋白酶-7的表达及意义

目的:研究基质金属蛋白酶-7(MMP-7)在食管鳞癌中的表达情况及其与肿瘤浸润和淋巴结转移的关系。方法:用免疫组织化学法检测75例食管鳞癌组织及其相应正常粘膜中MMP-7的表达。结果:食管鳞癌组织MMP-7基因表达(59/75,78.7％)高于正常组织(P<0.01)。浸润到外膜层食管鳞癌(37/45,82.2％)与浸润至粘膜下层鳞癌(5/10,50.0％)之间MMP-7的高表达有显著性差异(P<0.05),淋巴结转移组MMP-7高表达(27/29,93.1％)高于淋巴结未转移组(P<0.05)。结论:MMP-7的高表达与食管鳞癌浸润深度和淋巴结转移有关,可能成为食管鳞癌恶性生物学行为的指标。     

大肠癌组织中血管内皮生长因子的表达及意义

目的 探讨血管内皮生长因子（ＶＥＧＦ）在大肠癌组织中的表达及其与大砀癌发展的关系。方法 应用名单组织化学ＬＳＡＢ法检测５２你人大肠癌组织的ＶＥＧＦ表达,分析ＶＥＧＦ与大肠癌组织类类型、分化程度、Ｄｕｋｅｓ分期及淋巴结转移毕率随关大肠癌Ｋｕｋｅｓ分期的进展而增加,且Ｄｕｋｅｓ Ｃ期的ＶＥＧＦ表达率与ＤｕｋｅｓＡ期相比有显著性差异（Ｐ〈０．０５）,ＶＥＧＦ在有淋巴结转移组的４０．００％（Ｐ〈０．０１）     

环氧化酶-2和血管内皮生长因子在浸润性乳腺癌中的表达及其与淋巴结转移的关系研究

目的研究环氧化酶-2(Cox-2)和血管内皮生长因子(VEGF)在乳腺癌中的表达情况,探讨两者与淋巴结转移的关系。方法选择我院确诊的乳腺浸润导管癌手术标本92例为乳腺癌组,同时选取45例同期乳腺癌癌旁组织作为正常对照组。采用免疫组化SP法检测92例乳腺浸润癌中Cox-2、VEGF的表达情况。结果 Cox-2蛋白在乳腺癌旁对照组中的阳性表达率为0,在乳腺癌组织中的阳性表达率为68.5%,两组比较差异有统计学意义(P<0.05);Cox-2在淋巴结转移组与无淋巴结转移组中的阳性表达比较,差异有统计学意义(P<0.05)。VEGF蛋白在乳腺对照组中的阳性表达率为6.7%,在乳腺癌组织中的阳性表达率为90.2%,两组比较差异有统计学意义(P<0.05);VEGF在淋巴结转移组中的阳性表达率明显高于无淋巴结转移组,差异有统计学意义(P<0.05)。结论 Cox-2、VEGF蛋白在乳腺癌组织中高表达,且均与淋巴结转移有关,两者可能在乳腺癌的远处转移中起重要作用。     

宫颈鳞癌组织中pSTAT3、Cyclin D1、VEGF表达变化及意义

目的 观察宫颈鳞癌组织中磷酸化信号转导和转录激活因子3 （pSTAT3）、细胞周期素D1（Cyclin D1）及血管内皮生长因子（VEGF）蛋白的表达变化,并探讨其临床意义.方法 采用免疫组化SP法检测40例宫颈鳞癌（癌症组）、20例宫颈上皮内瘤变（CIN组）和15例正常宫颈鳞状上皮（对照组）组织中的pSTAT3、Cyclin D1和VEGF蛋白.结果 在癌症组及CIN组,pSTAT3过表达率分别为20.0％、62.5％ （P ＜0.05）,Cyclin D1过表达率分别为30.0％、72.5％ （P ＜0.05）,VEGF过表达率分别为40.0％、82.5％（P＜0.05）;对照组均无过表达者.pSTAT3、Cyclin D1蛋白过表达与宫颈鳞癌病理分级、临床分期及淋巴结转移有关（P均＜0.05）,VEGF蛋白过表达与宫颈鳞癌病理分级、淋巴结转移有关（P均＜0.05）.宫颈鳞癌组织中,pSTAT3与Cyclin D1及VEGF蛋白过表达呈正相关（r分别为0.531、0.618,P均＜0.05）.结论 宫颈鳞癌组织中pSTAT3、Cyclin D1及VEGF蛋白表达增高,三者共同参与了宫颈鳞癌的发生、发展.     

STAT5和WWOX在非小细胞肺癌中的表达及相关性研究

目的 探讨信号转导和转录激活因子5(STAT5)和WW结构域氧化还原酶(WWOX)在非小细胞肺癌(NSCLC)中的表达及相关性研究.方法 用免疫组化SP法检测40例手术切除的NSCLC组织和20例正常肺组织中STAT5和WWOX的蛋白表达.结果 STAT5蛋白在NSCLC中的表达明显高于正常肺组织,而WWOX在NSCLC中的表达明显低于正常肺组织(P<0.01);STAT5表达水平与NSCLC组织学类型有关,在腺癌组织中的表达明显高于鳞癌(P<0.01),WWOX的表达与淋巴结转移相关,有淋巴结转移的表达低于无淋巴结结转移(P<0.01);STAT5蛋白和WWOX蛋白在NSCLC组织中的表达呈负相关(P<0.05).结论 在NSCLC中存在STAT5的过度表达以及WWOX的失表达,STAT5表达水平与NSCLC组织学类型有关,而WWOX的表达与淋巴结转移相关.提示在NSCLC的发生机制中,WWOX可能通过抑制STAT5系统,促进细胞凋亡,起到抑癌作用.     

食管鳞癌中RECK和MMP-9蛋白表达的相关性及临床病理意义

目的:探讨RECK及基质金属蛋白酶-9(MMP-9)表达与食管癌发生、发展及浸润、转移的关系.方法:应用免疫组化SP法检测62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中RECK及MMP-9蛋白表达.采用X2检验进行统计学分析.结果:食管鳞癌组织中RECK和MMP-9蛋白表达与癌的组织学分级、浸润深度及淋巴结转移密切相关(χ2=10.422,8.550,4.751;χ2=8.447,14.333,5.373;均P＜0.05).在食管鳞癌癌变过程中RECK蛋白表达在癌组织、癌旁不典型增生组织及正常黏膜组织中的表达率依次增高,分别为59.7%,71.0%,85.5%,组间比较有明显差异(P＜0.01);而MMP-9蛋白在癌组织、癌旁不典型增生组织及正常黏膜组织中的表达率依次降低,分别为80.6%,80.6%,27.4%,组间比较有明显差异(P＜0.01).结论:RECK和MMP-9在食管癌的浸润、转移及黏膜上皮癌变过程中起重要作用,RECK及MMP-9的联合检测可望成为食管鳞癌早期诊断和判断预后的分子指标之一.     

食管鳞癌组织中VEGF-D表达及其与淋巴管密度和淋巴转移的关系

目的 研究食管鳞状细胞癌(ESCC)组织中血管内皮细胞生长因子D(VEGF-D)的表达情况及其与淋巴管密度(LVD)的关系,并探讨其与食管癌淋巴结转移的关系.方法 免疫组化SABC法检测55例手术切除的食管鳞状细胞癌组织、癌旁组织、正常组织中淋巴管密度和VEGF-D的表达水平,对结果进行统计分析.结果 (1)食管癌组织...     

Correlation of matrix metalloproteinase suppressor genes RECK, VEGF, and CD105 with angiogenesis and biological behavior in esophageal squamous cell carcinoma

AIM： To explore the expression of reversion inducing cysteine-rich protein with Kazal motifs （RECK）, vascular endothelial growth factor （VEGF） and endoglin （CD105） protein and its correlation with occurrence, development, invasion and metastasis in esophageal squamous cell carcinoma （ESCC）. METHODS： Streptavidin-peroxidase （SP） immunohistochemistry was used to detect expression of RECK and VEGF in 62 cases of ESCC, 31 cases of adjacent atypical hyperplastic epithelium and 62 cases of normal esophageal epithelium. CD105 Mb was used to assess microvessel density （MVD）. RESULTS： The expression of RECK was closely correlated with histological grade, infiltrative depth and lymphatic metastasis in ESCC （P 〈 0.05）. The expression of RECK decreased during cancer development： normal esophageal epithelium （85.5%, 53/62）, adjacent atypical hyperplastic epithelium （71.0%, 22/31）, and carcinoma （59.7%, 37/62）. There was a significant difference among the groups （P 〈 0.05）. The expression of VEGF protein was closely correlated with infiltrative depth and lymphatic metastasis in ESCC （P 〈 0.05）. The expression of VEGF protein increased during cancer development： normal esophageal epithelium （29.0%, 18/62）, adjacent atypical hyperplastic epithelium （54.8%, 17/31）, and carcinoma （67.7%, 42/62）. There was a significant difference among the groups （P 〈 0.05）. MVDCD105 increased in accordance with histological grade, butthere was no significant difference （grade Ⅰ, 36.92 ± 10.85; grade Ⅱ, 37.65 ± 9.50; and grade Ⅲ, 38.06 ± 12.19）. The MVDCD105 was closely correlated with infiltration and lymphatic metastasis in ESCC （P 〈 0.05）. The expression of RECK was inversely correlated with the expression of VEGF and CD105.CONCLUSION： RECK, VEGF and CD105 play important roles in the infiltration, metastasis and carcinogenesis in esophageal carcinoma. Angiogenesis in ESCC may be promoted by over-expression of CD105.     

血管内皮生长因子表达与食管鳞癌临床病理的关系

目的 了解食管鳞癌组织中血管内皮生长因子（VEGF）的变化及其与临床病理的关系。方法 采用ABC方法测定食管鳞癌高发区74例食管鳞癌组织中VEGF蛋白的表达。结果 74例食管鳞癌组织中VEGF阳性表达为60.8%（45/74例）,VEGF阳性表达与食管鳞癌组织中肿瘤细胞增殖、浸润和淋巴结转移有相关性。VEGF在食管鳞癌中的表达存在有异质性。结论 VEGF蛋白在食管鳞癌的增殖、浸润和转移过程中起重要作用。     

Expression of G3BP and RhoC in esophageal squamous carcinoma and their effect on prognosis

AIM： To investigate the expression and significance of G3BP and RhoC proteins in esophageal squamous carcinoma （ESC）.
METHODS： The expression of G3BP and Rhoc proteins in 80 cases of ESC was detected by immunohistochemistry. The relationship was studied between the expression of the two proteins and tumor size, differentiation degree, TNM stage, lymph node metastasis and prognosis of ESC.
RESULTS： The positive expression rate of G3BP in ESC was 71.25%; and the rate in the lymph node metastasis group was significantly higher than that in the nonlymph node metastasis group （Z = -2.283, P = 0.022）, but no relations were found between G3BP expression and tumor size, differentiation degree and TNM stage （P 〉 0.05）. The group with G3BP positive expression had shorter survival time than the group with G3BP negative expression （P = 0.000）. The positive expression rate of RhoC in ESC was 66.25%; and the rate in the lymph node metastasis group was significantly higher than that in the non-lymph node metastasis group （Z = -2.115, P 〈 0.05）, but no relations were found between RhoC expression and tumor size, differentiation degree and TNM stage （P 〉 0.05）. The RhoC positive expression group had a shorter survival time than the RhoC negative expression group （P 〈 0.001. The expression of G3BP protein correlated positively with the expression of RhoC in ESC tissues （rs = 0.656, P 〈 0.001）.
CONCLUSION： The expression of G3BP and RhoC protein is closely related to the lymph node metastasis and survival in ESC patients. G3BP and RhoC proteins can be considered as predictors of prognosis in ESC patients.     

Expression of human chorionic gonadotropin, CD44v6 and CD44v4/5 in esophageal squamous cell carcinoma

AIM: To study the relationship between the expression of human chorionic gonadotropin (HCG), CD44v6, CD44v4/5 and the infiltration, metastasis of esophageal squamous cell carcinoma. METHODS: By labeled streptavidin-biotin technique, the expressions of HCG, CD44v6, and CD44v4/5 in 42 patients with esophageal squamous cell carcinoma were examined. RESULTS: The positive rate of HCG expression in patients with lymph node metastasis was 85.71% (18/21), higher than that (57.14%, 12/21) in those without lymph node metastasis (P<0.05). The positive rate of CD44v6 expression was 71.43% (15/21) in lymph node metastasis group, and 38.09% (8/21) in non-metastasis group; there was a significant difference between the two groups (P<0.05). The positive rate of CD44v4/5 expression was 76.19% (16/21) in lymph node metastasis group, and 42.86% (9/21) in non-metastasis group; there was also a significant difference between them (P<0.05). From grade Ⅰ to grade Ⅲ in differentiation, the positive rate of HCG expression was 84.62% (11/13), 70.59% (12/17) and 58.33% (7/12), respectively; there was no significant difference among them (P<0.05). The positive rate of CD44v6 expression in grades Ⅰ-Ⅲ of cancer tissues was 76.92% (10/13), 52.94% (9/17), and 33.33% (4/12) respectively; there was no significant difference among them. The positive rate of CD44v4/5 expression in grades Ⅰ-Ⅲ of cancer tissues was 69.23% (9/13), 64.71% (11/17), and 41.67% (5/12) respectively; there was no significant difference among the three groups. There was no correlation between the positive rates of HCG and CD44v6, CD44v4/5 expression. Cancer cells in carcinomatous emboli and those infiltrating into vascular wall strongly expressed HCG, CD44v6, and CD44v4/5. CONCLUSION: Expression of HCG, CD44v6, and CD44v4/5 in esophageal squamous cell carcinoma is related to its infiltration and metastasis. HCG, CD44v6, and CD44v4/5 have different effects on the infiltration and metastasis of esophageal squamous cell carcinoma.     

食管鳞癌组织中S100A4和MMP2蛋白表达与临床病理学特征的关系

目的:探讨食管鳞癌组织和癌旁正常黏膜组织中S100A4和MMP2的表达及其与食管鳞癌临床病理因素之间的关系.方法:应用SP免疫组织化学技术检测100例食管鳞癌及其癌旁正常黏膜组织中S100A4和MMP2蛋白的表达.结果:食管鳞癌组织中S100A4和MMP2蛋白阳性率分别为52.00%和67.00%,明显高于癌旁正常黏膜组织26.00%和3 1.00%(P<0.01).二者在食管鳞癌中的表达呈正相关,且与癌组织的分化程度、浸润深度、淋巴结转移密切相关(P<0.05).结论:S100A4可能通过对MMP-2蛋白的调控在食管鳞癌的发生、发展及食管鳞癌的早期侵袭转移中起关键作用,有望成为评估食管癌预后的一个新的标志物.     

LOX、VEGF在胃癌进展中的作用关系

目的探讨赖氨酰氧化酶(lysyl oxidase,LOX)、血管内皮因子(vascular endothelial growth factor,VEGF)在胃癌进展中的作用关系。方法收集手术切除的新鲜胃癌组织65例,采用Western blotting方法检测胃癌组织中的LOX、VEGF蛋白表达,并比较二者在T1T2与T3T4胃癌组织、有无淋巴结转移胃癌组织中的关系。结果 (1)LOX在T3T4胃癌中的相对表达量为0.6003±0.1279,在T1T2胃癌中的相对表达量为0.4278±0.1437,差异有统计学意义(P0.05);(2)VEGF在T3T4胃癌中的相对表达量为0.6975±0.1639,在T1T2胃癌中的相对表达量为0.4263±0.1128,差异有统计学意义(P0.05);(3)LOX在伴有淋巴结转移的胃癌中的相对表达量为0.6827±0.1987,在无淋巴结转移的胃癌中的相对表达量为0.4235±0.1763,差异有统计学意义(P0.05);(4)VEGF在伴有淋巴结转移的胃癌中的相对表达量为0.6769±0.1659,在无淋巴结转移的胃癌中的相对表达量为0.4128±0.1471,差异有统计学意义(P0.05);(5)在T1T2和T3T4胃癌组织中,LOX与VEGF的表达水平呈正相关(r=0.735,P0.05),在有淋巴结转移和无淋巴结转移的胃癌组织中,LOX与VEGF的表达水平也呈正相关(r=0.914,P0.05)。结论 T3T4胃癌中LOX与VEGF的表达水平明显高于T1T2胃癌,在有淋巴结转移的胃癌组织中,LOX与VEGF的表达水平明显高于无淋巴结转移的胃癌组织中,且LOX与VEGF表达呈正相关;LOX与VEGF在胃癌的进展中有促进作用,并且相互协同。     

明胶酶B mRNA在食管癌组织中的表达

目的探讨明胶酶B mRNA在食管癌组织中表达的临床意义.方法用原位杂交法检测41例食管癌患者的癌组织及癌旁组织明胶酶B mRNA的表达.结果食管癌患者的癌组织明胶酶B mRNA的表达率(33/41)与癌旁组织的表达率(24/41)相比有显著差异(P＜0.05).有淋巴结转移食管癌患者的癌组织明胶酶B mRNA的表达率(18/19)与无淋巴结转移的表达率(15/22)相比有显著差异(P＜0.05).侵及浆膜层癌组织表达率(16/18)高于未侵及浆膜层者(17/23),但无统计学意义.结论明胶酶B在食管癌的侵袭和转移过程中可能具有重要作用.     

WDHD1蛋白在非小细胞肺癌中的表达及临床意义

目的探讨WDHD1蛋白在非小细胞肺癌发生,发展,浸润和转移中的作用。方法用免疫组化MaxVision法检测WDHD1蛋白在肺鳞癌,腺癌和正常肺组织中的表达。结果 WDHD1蛋白在非小细胞肺癌组织中的表达明显高于周围正常肺组织(P0.05),其表达与非小细胞肺癌临床分期(P0.05)、浸润范围(P0.05)、淋巴结转移(P0.05)和组织分化程度(P0.05)有关。结论 WDHD1蛋白在非小细胞肺癌中高表达,与非小细胞肺癌分化差、恶性程度、临床分期、淋巴结转移密切相关,在非小细胞肺癌的发生发展过程中可能起重要作用.     

食管癌中细胞粘附分子E-钙粘素和CD44变异体表达及其临床意义

目的　探讨细胞粘附分子E 钙粘素 (E CD)和CD44变异体 (CD44V6)的表达与食管癌临床病理及预后的关系。方法　对 5 8例食管癌标本应用SP免疫组化染色法进行E CD、CD44V 6检测 ,并对其中 42例进行术后 3年随访。结果　E CD低表达和CD44V 6高表达与食管癌的浸润转移、复发和预后相关 (P <0 .0 5 )。E CD低阳性表达率与食管癌细胞分化程度相关 (P <0 .0 5 )。食管癌中E CD和CD44V6的表达无相关性 (P >0 .0 5 )。结论　E CD和CD44V 6在食管癌中的表达与食管癌的发生发展、侵袭转移和预后密切相关 ,E CD和CD44V6在食管癌中的表达无相关性 ,在食管癌的发生、发展、侵袭转移中是 2个独立的调节因子 ,可视为预测侵袭转移和估计预后的重要参考指标     

MRP3、GST-π在食管鳞状细胞癌中的表达及与预后的关系

目的研究肿瘤耐药相关基因MRP3、GST-π在食管鳞状细胞癌组织中的表达及其与临床病理特征和预后的关系。方法应用免疫组化法检测76例食管鳞状细胞癌患者MRP3、GST-π的表达,同时回顾性研究这些表达与食管鳞状细胞癌发生、浸润及转移等临床病理参数的关系。应用Kaplan-Meier生存曲线和Cox回归模型分析其与患者预后的关系。结果食管鳞状细胞癌组织中,MRP3在不同临床分期、有或无淋巴结转移组之间阳性表达率的差异有统计学意义（P〈0.05）;GST-π在不同临床分期、不同浸润深度组之间阳性表达率的差异有统计学意义（P〈0.05）。单因素生存分析表明,MRP3、GST-π阳性表达组的生存率虽低于阴性表达组,但差异无统计学意义（P〉0.05）。Cox模型多因素分析表明,肿瘤临床分期是影响生存期的独立因素（P〈0.05）。结论检测MRP3、GST-π的表达对食管鳞状细胞癌化疗方案的制定、判断预后有重要的指导意义。