依达拉奉联合巴曲酶治疗脑梗死疗效观察

目的:观察依达拉奉联合巴曲酶治疗急性脑梗死的疗效及其安全性.方法:60例急性脑梗死患者分为依达拉奉组,依达拉奉加巴曲酶组,毎组30例,观察治疗后神经功能改善情况及血浆纤维蛋白原(FIB)的变化.结果:联合治疗组治疗后各时期ESS评分均显著高于依达拉奉组(P＜0.05).治疗后血浆FIB的水平显著低于治疗前(P＜0.01).联合组有2例丙氨酸转氨酶(ALT)轻度升高.结论:依达拉奉与巴曲酶联用能显著提高急性脑梗死的疗效,且无明显不良反应.     

依达拉奉联合巴曲酶治疗急性脑梗死的临床观察

目的观察依达拉奉和巴曲酶联合治疗急性进展型脑梗死的疗效。方法将60例急性进展型脑梗死患者随机分为两组各30例。联合组使用依达拉奉和巴曲酶静脉滴注,巴曲酶组单用巴曲酶。结果治疗14 d后,联合组和巴曲酶组的血纤维蛋白原水平、神经功能缺损评分间差异均有统计学意义(P<0.05)。联合组的临床疗效优于巴曲酶组,差异有统计学意义(P<0.01)。结论依达拉奉联合巴曲酶治疗急性脑梗死效果显著,无明显不良反应。     

依达拉奉联合巴曲酶治疗进展性脑梗死的临床疗效观察

目的研究依达拉奉联合巴曲酶治疗进展性脑梗死的临床疗效。方法将58例发病72 h内的进展性脑梗死患者随机分为联合组29例,对照组29例,联合组在常规治疗基础上应用依达拉奉和巴曲酶治疗,对照组在常规治疗基础上只应用巴曲酶治疗,并于治疗第14 d观察两组疗效及神经功能缺损程度评分(NDS)恢复情况。结果联合组29例中,显效率、总有效率分别为72.4%、89.6%,高于对照组显效率44.8%、总有效率68.9%,两组比较有显著性差异(P<0.05);两组NDS均较入院时降低(P<0.05),联合组较对照组降低更明显(P<0.05),有显著性差异。结论依达拉奉联合巴曲酶治疗进展性脑梗死能明显地提高临床疗效,改善神经功能缺损情况。     

依达拉奉治疗30例急性脑梗死的临床疗效观察

目的:依达拉奉治疗急性脑梗死的疗效.方法:将60例急性脑梗死患者随机分为治疗组和对照组,对照组采用常规治疗;治疗组在常规治疗基础上加用依达拉奉30 mg每日2次静脉滴注;共14天.观察两组患者治疗前后神经功能缺损程度评分(NDS)及不良反应.结果:治疗组及对照组NDS评分与治疗前相比均有显著改善(P<0.05～0.01);治疗组改善程度与对照组相比差异有显著性(P<0.01).两组均未发现明显不良反应.结论:依达拉奉治疗急性脑梗死疗效显著.     

依达拉奉联合巴曲酶治疗进展性脑梗死的疗效观察

目的 观察巴曲酶联合依达拉奉治疗进展性脑梗死的临床疗效.方法 90例进展性脑梗死随机分为治疗组(45例)及对照组(45例).治疗组在常规治疗基础上应用依达拉奉30mg静滴,2次/d,14d为一疗程,并在病程的第1 d、3 d、5 d分别用降纤酶10 U、5 U、5 U静滴;对照组在常规治疗的基础上于病程的第1 d、3 d、5 d分别用降纤酶10U、5U、5U静滴.结果 治疗组临床疗效明显优于对照组(P<0.01);治疗组神经功能缺损评分明显低于对照组(P<0.01),两组血液中纤维蛋白原含量均明显下降(P<0.05).结论 依达拉奉联合巴曲酶治疗进展性脑梗死疗效更好且安全.     

巴曲酶联合依达拉奉治疗急性脑梗死疗效观察

目的探讨观察巴曲酶（DF-521）联合依达拉奉治疗急性脑梗死的临床疗效,安全性和有效性。方法选取2010年5月-2012年5月来该院治疗急性脑梗死的100例患者,随机分成两组,即常规治疗组（对照组）和联合治疗组（治疗组）,对照组给予基本治疗,治疗组给予巴曲酶、依达拉奉,对两组临床疗效等进行分析。结果两种不同的方案在临床效果和神经功能缺损评分上差异有统计学意义,治疗组21d后总有效率为92％,明显优于对照组56％,差异有统计学意义（P〈0．05）。结论通过两组临床治疗观察发现,巴曲酶联合依达拉奉在治疗急性脑梗死方面安全有效,能明显减轻神经功能损伤。     

巴曲酶联合依达拉奉治疗进展型脑梗死的疗效分析

目的:观察巴曲酶联合依达拉奉治疗进展型脑梗死的临床疗效。方法:70例进展性脑梗死患者随机分成治疗组和对照组,各35例;2组均予常规治疗,对照组单用巴曲酶,治疗组应用巴曲酶联合依达拉奉。疗程结束后比较2组的疗效,以及治疗前、治疗后第7天和第15天进行神经功能评分。结果:治疗组第7天、第15天神经功能评分均显著低于对照组(P0.01),治疗组总有效率与对照组差异无统计学意义(P0.05)。结论:巴曲酶联合依达拉奉治疗能促进进展型脑梗死神经功能的恢复。     

依达拉奉联合丁苯肽治疗急性脑梗死的疗效观察

为观察依达拉奉联合丁苯肽治疗急性脑梗死的临床疗效,选取60例急性脑梗死患者随机分为两组,两组患者在给予改善微循环等常规治疗的基础之上,治疗组给予依达拉奉联合丁苯肽治疗,对照组单纯应用丁苯肽治疗,对比两组患者的临床疗效、治疗前后神经功能缺损程度评分(NIHSS).结果,治疗组总有效率80%,对照组总有效率33.3%(P<0.05);治疗组NIHSS评分减分幅度较对照组有明显改善,两组比较差异有统计学意义(P<0.05).依达拉奉联合丁苯肽治疗急性脑梗死能改善神经功能,是治疗急性脑梗死的有效措施之一.     

依达拉奉联合前列地尔治疗急性脑梗死43例疗效观察

目的:探讨依达拉奉联合前列地尔注射液治疗急性脑梗死的临床疗效.方法:将符合标准的80例急性脑梗死患者随机分成观察组(43例)和对照组(37例),对照组在常规治疗基础上给予丹参注射液及胞二磷胆碱注射液,观察组在常规治疗基础上给予依达拉奉和前列地尔注射液联合治疗.两组疗程均为14d,观察两组患者的神经功能缺损评分、日常活动能力评分、疗效与并发症.结果:两组患者神经功能缺损和日常活动能力评分均较治疗前明显改善(P<0.05),观察组相对于对照组更能改善神经功能缺损及提高生活能力(P＜0.05);观察组临床总有效率明显高于对照组,而并发症发生率明显低于对照组(P＜0.05).结论:依达拉奉联合前列地尔注射液治疗急性脑梗死疗效肯定,值得临床推广.     

依达拉奉联合巴曲酶治疗脑梗死临床疗效观察

目的：观察依达拉奉联合巴曲酶治疗急性脑梗死的临床疗效及安全性。方法：60例患者随机分为两组，分别采用单用巴曲酶及依达拉奉联合巴曲酶治疗。以疗程14、21、90d神经功能缺损评分及3个月时Barthel指数评分为标准观察疗效。结果：两组病人疗程14d、21、90d神经功能缺损评分及3个月时Barthel指数差异有显著性意义。治疗后两组血浆纤维蛋白原含量均显著降低，但无显著性差异（P〈0．05）。结论：依达拉奉联合巴曲酶治疗急性脑梗死安全有效，能显著改善患者的预后。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.