共查询到5条相似文献，搜索用时 93 毫秒
1.
目的探讨中药扶正祛邪药对——黄芪、莪术粗提物与奥沙利铂(L-OHP)联合应用抑制鼠结肠癌CT26 wild-type(CT26.WT)细胞的转移,调控CXCR3、CCR6 mRNA及蛋白表达的作用。方法首先构建BALB/c结肠癌原位移植瘤小鼠模型,之后运用L-OHP(0.1 mg/次,隔日1次)单独以及联合黄芪、莪术粗提物高、中、低剂量(6、3、1.5 g/kg)作用于结肠癌模型小鼠,观察模型小鼠肝转移情况及运用RT-PCR测定CXCR3、CCR6 mRNA表达,Western Blot检测CXCR3、CCR6蛋白表达。结果 L-OHP单独应用和联合中药应用均能抑制原位移植瘤体的增大,阳性药抑制原位移植瘤生长的效果最好(P0.01)。联合用药能降低肝转移灶的发生(P0.05)。与空白对照组相比,阳性组及联合用药低剂量组CXCR3及CCR6 mRNA的表达显著下调(P0.01),联合用药能不同程度下调CXCR3、CCR6蛋白表达(P0.05,P0.01)。结论黄芪、莪术联合化疗药抗肿瘤转移机制可能与下调CXCR3、CCR6 mRNA及蛋白相关。 相似文献
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目的:明确CXCL10/CXCR3轴与白癜风发病相关性,探索甘草酸可能参与免疫调节的过程。方法:选取18例健康对照者(健康对照组)、40例白癜风患者为研究对象。随机将40例白癜风患者分为对照组和试验组,每组各20例,对照组给予补肾活血方治疗,试验组给予补肾活血方联合复方甘草酸苷片治疗,两组均治疗2个月。治疗前,采用ELISA法检测健康对照者和白癜风患者CXCL10表达水平,采用流式法检测健康对照者和白癜风患者CXCR3~+CD4~+T细胞、CXCR3~+CD8~+T细胞表达频率;治疗后采用ELISA法检测白癜风患者和健康对照者外周血清中CXCL10表达水平,同时观察不同组间CXCL10表达差异。结果:与健康对照者比较,治疗前白癜风患者外周血清中CXCL10表达水平显著增高(P0.001),治疗前白癜风患者CXCR3~+CD4~+在CD3~+细胞和CD4~+T细胞比值均显著升高(P0.001),CXCR3~+CD8~+在CD3~+细胞和CD8~+T细胞比值亦显著升高(P0.001)。与入组时比较,2个月后健康对照者外周血清中CXCL10表达水平无明显变化(P=0.442);治疗后,白癜风患者外周血清中CXCL10表达水平显著降低(P0.01),但仍明显高于2个月后复测健康对照者(P0.001);与对照组比较,试验组白癜风患者外周血清中CXCL10表达水平下降更明显(P0.05)。结论:CXCL10/CXCR3轴与白癜风发病相关,甘草酸可能通过抑制CXCL10表达参与免疫调节过程。 相似文献
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目的 探讨黄芪-莪术影响C5a介导JAK2/STAT3通路调控肿瘤微环境中Th17/Treg细胞平衡,从而阐明其在此途径抑制肿瘤进展的相关机制。方法 40只雄性C57BL/6小鼠按随机数字表法分为空白对照组、模型对照组、中药干预组、阳性对照药(PMX53)组,每组各10只。腋下接种Lewis细胞建立肺癌小鼠模型,于接种后第3天开始,中药干预组按8.2 g/kg剂量给予中药浓煎液灌胃,模型对照组和PMX53组予等容积灭菌双蒸水灌胃,连续14 d; PMX53组分别于第3、6、9、12、15天按1mg/kg剂量给予腹腔注射PMX53,模型对照组和中药干预组同时腹腔注射等容PBS溶液。每2 d测算各组小鼠肿瘤体积、绘制肿瘤生长曲线;于第15天处死,剖取瘤块,ELISA法检测血清C5a、IL6、IL-10、IL-17、TGF-β等因子水平,流式细胞术检测并计算外周血和肿瘤组织中Th17/Treg细胞比例,Western Blot和Real-time PCR法检测肿瘤组织中JAK2/STAT3信号通路相关蛋白及mRNA表达。结果 与模型对照组比较,中药干预组和PMX53组肿瘤生长较缓慢,补体C5... 相似文献
4.
目的 通过蓝莓联合益生菌减少CXCL10/CXCR3的表达对非酒精性脂肪肝(NAFLD)的影响及作用机制.方法 清洁级SD大鼠30只分为正常组(normal group,N组)、模型组(model group,M组)、蓝莓组(blueberry group,B组)、益生菌组(probiotics group,P组)和蓝莓益生菌组(blueberry+ probiotics group,BP组).除N组(100％普通饮食)外,其余大鼠均采用复合高脂饲料(88.2％普通饲料+10％猪油+1.5％胆固醇+0.2％胆酸钠)制备NAFLD模型共12周.确认造模成功后再将剩余M组大鼠随机分为B组(蓝莓原浆1.5 mL/(100 g·d)灌胃)、P组(益生菌溶液1.5 mL/d灌胃)及BP组(蓝莓益生菌原液1.5 mL/100 g体重灌胃),并给普通饮食,共观察8周.结果 BP组与M组、B组、P组比较:肝脏指数、血清丙氨酸氨基转移酶、门冬氨酸氨基转移酶、总胆固醇、三酰甘油均差异有统计学意义(P＜0.01).免疫组化:BP组的CXCL10/CXCR3表达较M组、B组、P组减少(P＜0.01).RT-PCR:BP组的CXCL10/CXCR3表达与M组、B组、P组比较显著降低(P＜0.01).Western blot:BP组的CXCL10/CXCR3表达较M组、B组、P组显著降低(P＜0.01).结论 蓝莓联合益生菌对比单独使用蓝莓或益生菌能有效改善NAFLD的病理组织结构,减轻肝细胞脂肪变性,其机制可能是蓝莓与益生菌联合可减轻CXCL-10诱发的炎症反应,下调三酰甘油相关基因的表达,减少脂质沉积,是改善NAFLD的一个辅助治疗方案. 相似文献
5.
JIANG Pei-juan LIN Qi-de BAO Shi-min ZHAO Ai-min ZHANG Yu XIAO Shi-jin 《中华医学杂志(英文版)》2009,122(4):390-395
Background Previous studies have shown that local immune cells in the feto-maternal interface are recruited from peripheral blood, and that chemokines and their receptors play an initial and key role in this recruitment process. In this study, we aimed to determine whether spontaneous abortion is associated with the expression of chemokine receptors CCR3, CCR5, and CXCR3 on CD4^＋ T cells.
Methods Peripheral blood, spleen, and thymus were collected from the spontaneous abortion mouse model CBA/JxDBA/2 （SA group, n=14）, the normal pregnant mouse model CBA/JxBALB/c （NP group, n=13）, and normal non-pregnant CBA/J mice （NNP group, n=11）. The number of chemokine receptors CCR3, CCR5, and CXCR3 expressed on CD4^＋ T cells was measured by double-label flow cytometry （FCM） method.
Results In peripheral blood, the SA group had significantly lower CCR3 expression （P 〈0.01） and higher CCR5 and CXCR3 expression （P 〈0.01） on CD4^＋ T cells than did the NP group. But comparing these chemokines between the SA and NNP groups, there was no significant difference （P 〉0.05）. In spleen, the SA group expressed significantly lower CCR3 expression （P 〈0.01） and higher CCR5 and CXCR3 expression （P 〈0.05） on CD4^＋ T cells than did the NP group. When compared with the NNP group, the SA group had significantly higher CCR3 expression （P 〈0.01）, but was not statistically different with regards to the other two chemokines （P 〉0.05）. In thymus, the SA group had significantly lower CCR3 expression （P 〈0.05） and higher CXCR3 expression （P 〈0.05） on CD4^＋ T cells than the NP group, with no significant difference in CCR5 expression （P 〉0.05）. Compared with the NNP group, the SA group had higher CCR3 expression （P 〈0.01）, but there was no statistical difference in CXCR3 and CCR5 expression （P 〉0.05） between the two groups.
Conclusion The abnormal expression of CCR3, CCR5 and CXCR3 on CD4^＋ T cells may play an important role in the pathogenesis of spontaneous abortion. 相似文献
Methods Peripheral blood, spleen, and thymus were collected from the spontaneous abortion mouse model CBA/JxDBA/2 （SA group, n=14）, the normal pregnant mouse model CBA/JxBALB/c （NP group, n=13）, and normal non-pregnant CBA/J mice （NNP group, n=11）. The number of chemokine receptors CCR3, CCR5, and CXCR3 expressed on CD4^＋ T cells was measured by double-label flow cytometry （FCM） method.
Results In peripheral blood, the SA group had significantly lower CCR3 expression （P 〈0.01） and higher CCR5 and CXCR3 expression （P 〈0.01） on CD4^＋ T cells than did the NP group. But comparing these chemokines between the SA and NNP groups, there was no significant difference （P 〉0.05）. In spleen, the SA group expressed significantly lower CCR3 expression （P 〈0.01） and higher CCR5 and CXCR3 expression （P 〈0.05） on CD4^＋ T cells than did the NP group. When compared with the NNP group, the SA group had significantly higher CCR3 expression （P 〈0.01）, but was not statistically different with regards to the other two chemokines （P 〉0.05）. In thymus, the SA group had significantly lower CCR3 expression （P 〈0.05） and higher CXCR3 expression （P 〈0.05） on CD4^＋ T cells than the NP group, with no significant difference in CCR5 expression （P 〉0.05）. Compared with the NNP group, the SA group had higher CCR3 expression （P 〈0.01）, but there was no statistical difference in CXCR3 and CCR5 expression （P 〉0.05） between the two groups.
Conclusion The abnormal expression of CCR3, CCR5 and CXCR3 on CD4^＋ T cells may play an important role in the pathogenesis of spontaneous abortion. 相似文献