急性脑梗塞病人红细胞免疫功能的变化及冬虫夏草治疗前后观察

测定４５例急性脑梗塞病人红细胞免疫功能，包括红细胞Ｃ＿３ｂ受体花环率（ＲＲＣＦ）、红细胞ＳＰＡ花环率（ＲＲＳＦ）、红细胞混合花环率（ＲＭＲＦ）、及裸红细胞（ＲＮＲ），并与正常人进行了对照，结果发现脑梗塞病人ＲＲＣＦ明显降低（Ｐ＜０．００１），而ＲＲＳＦ明显增高（Ｐ＜０．００１），表明脑梗塞病人存在红细胞免疫功能的异常。应用冬虫夏草进行治疗，３０天后，随着临床症状的恢复，红细胞免疫功能亦明显恢复，ＲＲＣＦ较用药前增高（Ｐ＜０．００１），ＲＲＳＦ则降低（Ｐ＜０．００１），提示冬虫夏草具有改善机体免疫功能的作用。     

缺铁性贫血和巨幼细胞贫血患者红细胞免疫功能的变化及其临 …

目的：探讨缺铁性贫血（ＩＤＡ）和巨幼细胞贫血（ＭＡ）患者红细胞免疫功能的变化及其临床意义。方法：采用红细胞酵母菌花环试验检测了２６例ＩＤＡ和１４例ＭＡ患者红细胞免疫功能。结果：红细胞Ｃ３ｂ受体花环率（ＲＣＲ）及红细胞促中性粒细胞吞噬率（ＥＲＰＭ）显著下降，红细胞免疫复合物花环率（ＲＩＣＲ）、红细胞Ｃ３ｂ受体花环抑制率（ＲＦＩＲ）及红细胞Ｃ３ｂ受体花环增强率（ＲＦＥＲ）无明显改变，铁剂、叶酸及维生素     

缺铁性贫血和巨幼细胞贫血患者红细胞免疫功能的变化及其临床意义

目的：探讨缺铁性贫血（ＩＤＡ）和巨幼细胞贫血（ＭＡ）患者红细胞免疫功能的变化及其临床意义。方法：采用红细胞酵母菌花环试验检测了２６例ＩＤＡ和１４例ＭＡ患者红细胞免疫功能。结果：红细胞Ｃ３ｂ受体花环率（ＲＣＲ）及红细胞促中性粒细胞吞噬率（ＥＲＰＭ）显著下降，红细胞免疫复合物花环率（ＲＩＣＲ）、红细胞Ｃ３ｂ受体花环抑制率（ＲＦＩＲ）及红细胞Ｃ３ｂ受体花环增强率（ＲＦＥＲ）无明显改变，铁剂、叶酸及维生素Ｂ１２治疗２～３个月后，ＲＣＲ和ＥＲＰＮ恢复正常。结论：ＩＤＡ和ＭＡ患者存在可逆性的红细胞免疫功能损害。     

大蒜素对恶性肿瘤患者红细胞免疫功能的影响

目的：探讨大蒜素对恶性肿瘤患者红细胞免疫功能的影响。方法：用红细胞Ｃ３ｂ受体花环试验（ＲＣＲＲ）、红细胞免疫复合花环试验（ＲＩＣＲ）、红细胞免疫粘附肿瘤细胞花环试验（ＴＲＣＲ）检测３０例恶性肿瘤患者服用大蒜素前、后１月、２月、３月及３０例正常对照组的红细胞免疫功能。结果：恶性肿瘤患者红细胞Ｃ３ｂ受体花环率及红细胞免疫站附肿瘤细胞花环经显示低于对照组（Ｐ〈０．０５）。而红细胞免疫复合物花环率显著高于     

慢性肺心病红细胞免疫功能与氧自由基的关系

目的：研究慢性肺心病（ＣＣＰ）患者红细胞免疫功能变化及其机制。方法：检测ＣＣＰ患者急性发作期２８例，缓解期２０例的红细胞免疫功能、血清丙二醛（ＭＤＡ）及红细胞超氧化物歧化酶（ＳＯＤ）等指标。结果：ＣＣＰ患者的红细胞Ｃ３ｂ受体花环率（ＣＲ３ｂＲＲ）降低，红细胞免疫复合物花环率（ＲＩＣＲ）增高，ＳＯＤ降低，ＭＤＡ增高，与正常对照组碾 明显性，且ＲＣ３ｂＲＲ与ＳＯＤ呈正相关，与ＭＤＡ呈负相关。结论：ＣＣ     

非胰岛素依赖型糖尿病及糖尿病肾病患者红细胞免疫功能的变化

本文检测３４例非胰岛素依赖型糖尿病（２例ＤＭ）患者及３１例糖尿病肾病（ＤＮ）患者的红细胞免疫功能,并与４０例健康人进行对照,结果发现２型ＤＭ及ＤＮ患者红细胞Ｃ３ｂ受体花环率（ＲＢＣ·Ｃ３ｂＲＲ）红细胞免疫促进因子（ＲＦＥＲ）显著下降,红细胞免疫复合物花环率（ＲＢＣ·ＩＣＲＲ）红细胞免疫抑制因子（ＲＦＩＲ）显著升高,ＲＢＣ·Ｃ３ｂＲＲ与肌酐呈负相关,ＲＢＣ·ＩＣＲＲ,ＲＦＩＥＲ与肌酐呈正相关,提示２     

慢性肺心病红细胞免疫功能与氧自由基的关系

目的：研究慢性肺心病（ＣＣＰ）患者红细胞免疫功能变化及其机制。方法：检测ＣＣＰ患者急性发作期２８例，缓解期２０例的红细胞免疫功能、血清丙二醛（ＭＤＡ）及红细胞超氧化物歧化酶（ＳＯＤ）等指标。结果：ＣＣＰ患者的红细胞Ｃ３ｂ受体花环率（ＲＣ３ｂＲＲ）降低，红细胞免疫复合物花环率（ＲＩＣＲ）增高，ＳＯＤ降低，ＭＤＡ增高，与正常对照组比较差异有显著性，且ＲＣ３ｂＲＲ与ＳＯＤ呈正相关，与ＭＤＡ呈负相关。结论：ＣＣＰ患者存在红细胞免疫功能低下和氧自由基代谢失调，二者是并行发生的，又相互作用与影响，二者之异常很可能继发于呼吸系感染，控制感染可使二者改善     

妊娠高血压综合征患者红细胞免疫功能的观察

对４０例妊娠高血压综合征患者和３０例正常孕妇进行了红细胞Ｃ＿３ｂ受体（Ｃ＿３ｂＲ）花环试验、免疫复合物（ＩＬ）花环试验及红细胞免疫粘附（ＲＣＩＡ）调节因子（ＲＣＩＡ促进因子和ＲＣＩＡ抑制因子）活性的测定。结果：妊高征患者Ｃ３＿ｂＲ花环率及ＩＣ花环率均低于正常孕妇（Ｐ＜０．０１），ＲＣＩＡ抑制因子活性明显高于正常孕妇（Ｐ＜０．００１）。提示：妊高征患者红细胞免疫功能原发低下。其原因可能与ＲＣＩＡ抑制因子活性增强有关。     

慢性阻塞性肺疾病患者红细胞免疫功能的研究

目的：研究慢性阻塞性肺疾病（ＣＯＰＤ）患者红细胞免疫功能的变化以及与ＣＯＰＤ发病的关系。方法：对８２例ＣＯＰＤ缓解期患者与其中５０例急性加重期患者及４０例正常对照者测定其Ｃ３ｂ受体花环率（Ｃ３ｂＲＲ）。免疫复合物花环率（ＩＣＲ）、Ｃ３ｂ受体花环促进率（ＲＦＥＲ）、Ｃ３ｂ受体花环抑制率（ＲＦＩＲ）。结果：ＣＯＰＤ缓解期与正常对照组比较、急性加重期与缓解期比较,Ｃ３ｂＲＲ与ＲＦＥＲ降低,ＩＣＲ与ＲＦＩＲ升高。差异均具显著性。结论：ＣＯＰＤ患者红细胞免疫功能降低,自身调控系统失衡可能是反复肺部感染的结果或是机体对ＣＯＰＤ易感的内在因素之一。     

股骨头缺血坏死病人红细胞免疫功能及其调控因子的变化

检测了４２例股骨头缺血坏死（ＡＮＦＨ）病人红细胞免疫功能及其调控因子的变化。结果表明，ＡＮＦＨ病人红细胞Ｃ３ｂ受体花环率（ＲＢＣ－Ｃ３ｂＲＲ）及红细胞Ｃ３ｂ受体促进率（ＲＦＥＲ）明显低于对照组，差异有极显著性（Ｐ＜０．００１）；而红细胞免疫复合物花环（ＲＢＣ－ＩＣＲ）、红细胞Ｃ３ｂ受体抑制率（ＲＦＩＲ）和血清循环免疫复合物（ＣＩＣ）明显高于对照组，差异有极显著性（Ｐ＜０．００１）。提示ＡＮＦＨ可能与红细胞免疫功能降低及红细胞免疫调控系统失调有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.