A型肉毒素在治疗面部动力性皱纹中的应用体会

目的: 观察A型肉毒素治疗面部皱纹的美容效果。方法: 对以美容为首要目的的面部皱纹患者采用A型肉毒素注射治疗。结果: A型肉毒素治疗面部皱纹效果好,创伤小。结论: A型肉毒素治疗面部皱纹安全、有效。     

除皱肽联合A型肉毒素改善面部皮肤皱纹的临床应用

目的观察基因除皱肽与A型肉毒素在治疗面部皱纹中的临床应用效果。方法面部消毒后在面部皱纹明显处周围标记A型肉毒素注射点，1周后用除皱肽沿皱纹皮内注射，每周2次，4次为一疗程。结果共治疗78例，术后随访2年，有效率达98％，效果满意。结论基因除皱肽联合A型肉毒素在治疗面部皱纹方面作用互补，延长治疗效果，临床效果良好。     

应用A型肉毒素对称注射法在面部除皱的体会

目的探讨A型肉毒素对称注射法在面部除皱中的应用优势．方法采用A型肉毒素以面中线为中轴对称注射面部皱纹，接受治疗的患者68例，其中额横纹、眉间纹、鱼尾纹62例，鼻唇沟纹、鼻背纹6例．结果68例患者面部动力性皱纹完全消失，有效率100％，无1例出现眉下垂、睑外翻、面部不对称等不良反应和并发症．结论A型肉毒素对称注射治疗面部动力性皱纹，操作方法简单、安全有效．     

A型肉毒素在面部除皱中的临床应用研究

目的:探讨A型肉毒素注射在清除面部动态皱纹的疗效及应用价值。方法:用1 ml结核菌素注射器,30号特制针头进行面部肌肉注射,根据个人皱纹的分布范围、多少调整注射不同点位,每点0.1ml(含5.0U),不需麻醉。结果:除9例患者曾出现轻度瘀斑、水肿,眉型改变、眉下垂,未见红疹、脱皮、恶心、头痛、头部紧绷感等不适外,其余大多数病例取得较好的效果,总有效率93%。结论:局部注射A型肉毒素治疗面部皱纹是一种安全有效的方法。     

A型肉毒素治疗面部皱纹11例

目的观察A型肉毒素治疗面部皱纹的疗效.方法:使用兰洲生物制品所生产的治疗用A型肉毒素额部注射.结果:皱纹舒展、消失,皮肤变平坦,治疗有效.结论:A型肉毒素除皱安全、筒便、有效.     

A型肉毒素治疗面部动静态皱纹的疗效观察

目的：观察局部点状注射A型肉毒素去除面部动静态皱纹及皮肤饱满度临床效果。方法：本组病例64例,其中静态皱纹40例,额纹18（43%）,鱼尾纹13（32%）,川字纹6例（15%隍,鼻背纹3例（7%）。动态皱纹24例,额纹16例（67%）,鱼尾纹8例（33%）。所有患者均实行局部多点注射,药物浓度4单位/0.1毫升,根据皱纹情况调整药量及注射点,记录皱纹变化,皮肤饱满度,药物维持时间,不良反应。结果：A型肉毒素对动态皱纹显效率100%,静态皱纹显效率94.2%。肌肉麻痹持续时间8~12个月,平均10个月。不良反应：头痛头晕1例,局部水肿,青紫3例症状3~5日消失,无全身不良反应。结论：A型肉毒素对面部动静态皱纹治疗安全,迅速,是有效面部除皱方法。     

联合A型肉毒素和自身脂肪组织祛除面部皱纹

目的 观察应用A型肉毒素和自身脂肪组织祛除面部皱纹效果.方法 采用A型肉毒素多点注射法和自身脂肪在皱纹真皮下填充术治疗156例面部皱纹患者.结果 156例患者中,半年后回访满意148例,满意率94.33%.结论 A型肉毒素和自身脂肪祛除面部皱纹是一种操作简单,安全有效的方法 .     

A型肉毒素用于面部除皱的体会

面部皱纹的出现与皮肤老化有关，是人到了一定年龄的必然产物。皱纹使人显得苍老，因此推迟或减轻皱纹的出现便成为人们的愿望。面部除皱有很多方法，在美容皮肤学科领域最常见的有胶原注射，化学剥脱术，皮肤磨削术和激光重塑术等，均属于创伤性方法。国内外已有不少有关肉毒素除皱的文献报道。2001年10月～2005年12月，笔者应用A型肉毒素局部注射面部除皱共176例，现总结报道如下。     

A型肉毒素在面部除皱的应用及其并发症的防治

目的A型肉毒素治疗面部皱纹的应用及并发症的防治。方法在产生额纹,眉间纹,鱼尾纹,鼻唇沟纹,口周纹等处注射适量A型肉毒素。结果掌握正确的注射方法可在面部除皱获得良好的美容效果,并防止并发症的发生。结论A型肉毒素在面部除皱的效果肯定、安全。     

A型肉毒素封闭治疗面肌和眼睑痉挛的临床研究(附60例临床分析)

目的：探讨A型肉毒素对面肌或眼睑痉挛的疗效。方法：对34例一般疗法无效的面肌痉挛、26例眼睑痉挛患者，局部注射A型肉毒素，并判断疗效，随访6～14个月。结果总有效率有100％。结论：局部注射A型肉毒素是治疗面肌和眼睑痉挛的有效而安全的新方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.