Y-chromosome short tandem repeat (STR) haplotypes in a Campania population sample.

Given the lack of information about Y haplotypes for Campania (southern Italy), we analyzed eight Y short tandem repeats in a sample of males from this region with the aim of establishing a Y-haplotype database that can be used for forensic purposes. The eight Y short tandem repeats were amplified by two PCR multiplex reactions: multiplex A for loci DYS19, DYS385, DYS392 and DYS393, and multiplex B for DYS389 I and II, DYS390, DYS391 and DYS385. The proportion of unique haplotypes was 100% (108 Y-chromosome haplotypes in 108 unrelated males) and the haplotype diversity discrimination index was 0.99. These data reflect the high heterogeneity of male lineage in our population and are similar to those obtained in other regions of southern Italy.     

引物延伸预扩增后STR分型在鉴定母血中胎儿有核红细胞的应用

目的 探讨一种鉴定母血中胎儿有核红细胞（NRBC）的方法，为无创性产前基因诊断创造必要条件。方法 联苯胺染色识别孕妇外周血中NRBC，经显微操作获取，并以引物延伸预扩增（PEP）对单个NRBC进行全基凶组扩增后，用短串联重复序列（STR）分析其基因型，与父母基因型比对，确定该细胞的来源。结果 28例轻型β地中海贫血孕妇外周血样本中每例发现NRBC4～13个／5ml，经鉴定每例有胎儿NRBC2～8个／5ml，约43．6％的NRBC来源于胎儿。结论 PEP后STR基因型分析能有效鉴定孕妇外周血中NRBC的来源，使应用单个胎儿NRBC进行产前基因诊断成为可能。     

中国朝鲜族人群6个短串联重复序列位点的遗传多态性

背景：许多学者对不同国家、地区、民族人群的短串联重复序列多态性进行了研究报道,但朝鲜族人群短串联重复序列位点的多态性如何？目的：了解中国朝鲜族人群D16S539,D7S820,D13S317,CSF1PO,TPOX,THO1 6个短串联重复序列位点的遗传多态性分布,获得相应多态位点的群体遗传学数据.设计：单一样本观察.单位：牡丹江医学院生物教研室和DNA分析检测室.材料：收集2001-01/02牡丹江地区无血缘关系的朝鲜族个体100份血样.方法：应用聚合酶链反应扩增片段长度多态性分析方法对100名无血缘关系的朝鲜族个体进行样本基因型检测.主要观察指标：D16S539,D7S820,D13S317,CSF1PO,TPOX,THO1 6个短串联重复序列位点的样本基因型.结果：①D16S539基因座,观察到6个等位基因,18种基因型.②D7S820基因座,观察到7个等位基因,22种基因型.③D13S317基因座,观察到7个等位基因,23种基因型.④CSF1PO基因座,观察到6个等位基因,16种基因型.⑤TPOX基因座,观察到6个等位基因,11种基因型.⑥THO1基因座,观察到5个等位基因,12种基因型.结论：6个位点等位片段多态性分布均符合Hardy-Weinberg平衡定律.且具有较高的杂合度,所得到的等位基因频率等数据可以为中国朝鲜族人群遗传学研究提供依据.     

Use of X-linked short tandem repeat loci in routine parentage casework

BACKGROUND: Dealing with genetic inconsistencies in parentage testing, especially in motherless cases, remains a continual difficulty. STUDY DESIGN AND METHODS: Four difficult cases, comprising two trios and two duos, were selected from routine parentage testing casework. In these, relatively low combined paternity indices were observed as a result of few discrepant loci that were treated as being due to paternal mutations. An additional eight short tandem repeat (STR) loci along the X chromosome were studied in the alleged father and female child to try and help resolve these cases. RESULTS: In all four cases, the X chromosome haplotypes in the alleged father were different from those in the child, showing decisively that the alleged father could be excluded from being the biologic father of the child. CONCLUSION: In recent times the study of X chromosome haplotypes has been shown to be useful in parentage testing where the alleged father is absent and where only his close relatives are available for testing. This work demonstrates that such studies can also prove valuable in the testing of standard trios and duos in cases where there only a few genetic inconsistencies amongst the loci tested, making it difficult to distinguish between paternal mutations and a close relative of the alleged father being the biologic father.     

Determination of sibship by PCR-amplified short tandem repeat analysis in Taiwan

BACKGROUND: Sibship determination for any two persons whose parents have died is one of the most fundamental issues of personal identification, second only to those of a parent-child relationship. STUDY DESIGN AND METHODS: By automated fluorescence analysis of a PCR-amplified short tandem repeat (STR) system in conjunction with capillary electrophoresis, a panel of up to 15 polymorphic, autosomal, unlinked STR loci was used to investigate sibship index (SI) values in a cohort of 126 true sibling pairs. These SI values were then compared with those of 126 random pairs. RESULTS: The 15-loci STR panel provides a cumulative power of exclusion of 0. 9999997. Of the 126 random pairs, 124 (98.4%) had cumulative sibship indices (CSIs) of <1.0, and none had a CSI of >3.0 (median, 0.0101; range, 0.0000003-2.5376). In contrast, 107 (85%) of the 126 sibling pairs had a CSI of >100 (median, 5,579.9853; range, 0.0747-9,406,829, 249.8461). However, five pairs (4%) of the sibling group had a CSI of <3.0. True sibship was confirmed for this particular group by additional paternity testing and mitochondrial DNA sequence analysis. Among a total of 1890 observations (15 loci x 126 pairs), two alleles per locus were shared 760 times (40.21%) (mean, 6.03 loci; range, 1-10) in the sibling group, but only 192 times (10.16%) in the random group (mean, 1.52 loci; range, 1-5) (p<0.001). No alleles were shared 696 times (36.83%) in the unrelated pairs, as compared to 176 times (9.31%) in the sibling group (p<0.001). A polarized distribution was not noted in the sharing of single alleles in either the random or the sibling group: 1002 observations (53.02%) and 954 observations (50.48%), respectively. CONCLUSION: Highly polymorphic STR analysis can be discriminative in most sibship determinations, and the sharing of two alleles per locus is most informative in indicating sibship. Complementary mitochondrial DNA sequence analysis is mandatory in a few cases to exclude or establish true sibship when CSIs are equivocal and neither parent is available.     

两例短串联重复序列三带型等位基因的遗传多态性分析

目的 本文分析亲子鉴定常用STR基因座的三带型等位基因特点. 方法 用Chelex法提取3 600例亲子鉴定检案(含8 734个个体)血液中的基因组DNA,利用美国ABI公司AmpFISTRR(R) IdentifilerTM plus试剂盒进行复合荧光PCR扩增确定STR基因座的遗传图谱(ABI 3500Dx遗传分析仪),若出现异常峰型则采用PowerPlex(R) 21系统进行复查验证和采用Yunis技术进行淋巴细胞染色体核型分析. 结果 2个检案分别在D18S51和FGA基因座上出现三带型等位基因现象(均属于Ⅰ型),总检出率为0.229％(2/8 734),携带三带型等位基因的个体经染色体核型分析均未发现三体综合征. 结论 三带型等位基因结果较少见,判读须慎重,应采用不同试剂盒进行验证以获得准确分型.     

中国朝鲜族人群6个短串联重复序列位点的遗传多态性

背景许多学者对不同国家、地区、民族人群的短串联重复序列多态性进行了研究报道,但朝鲜族人群短串联重复序列位点的多态性如何?目的了解中国朝鲜族人群D16S539,D7S820,D13S317,CSF1PO,TPOX,THO1 6个短串联重复序列位点的遗传多态性分布,获得相应多态位点的群体遗传学数据.设计单一样本观察.单位牡丹江医学院生物教研室和DNA分析检测室.材料收集2001-01/02牡丹江地区无血缘关系的朝鲜族个体100份血样.方法应用聚合酶链反应扩增片段长度多态性分析方法对100名无血缘关系的朝鲜族个体进行样本基因型检测.主要观察指标D16S539,D7S820,D13S317,CSF1PO,TPOX,THO1 6个短串联重复序列位点的样本基因型.结果①D16S539基因座,观察到6个等位基因,18种基因型.②D7S820基因座,观察到7个等位基因,22种基因型.③D13S317基因座,观察到7个等位基因,23种基因型.④CSF1PO基因座,观察到6个等位基因,16种基因型.⑤TPOX基因座,观察到6个等位基因,11种基因型.⑥THO1基因座,观察到5个等位基因,12种基因型.结论6个位点等位片段多态性分布均符合Hardy-Weinberg平衡定律.且具有较高的杂合度,所得到的等位基因频率等数据可以为中国朝鲜族人群遗传学研究提供依据.     

多位点串联重复序列分析方法在嗜麦芽窄食单胞菌分子分型中的应用

目的 探讨多位点串联重复序列分析方法(multiple-locus variable-number tandem repeat analysis, MLVA)在嗜麦芽窄食单胞菌分型中的应用。 方法 选用文献报道的12个嗜麦芽窄食单胞菌串联重复序列位点及引物,采用聚合酶链反应(PCR)和琼脂糖凝胶电泳,根据凝胶电泳图谱计算出各位点的串联重复单元拷贝数,通过BioNumerics(Version 4.0, Applied Maths BVBA, Belium)聚类分析。 结果 设置12个位点串联重复单元拷贝数100％的相似性为判断标准,可将106株嗜麦芽窄食单胞菌分为34个MLVA基因型,流行病学上有关联的菌株具有相同的基因型。设置12个位点串联重复单元拷贝数45％相似性为判断标准,将106株菌株分为11个群,相同的地域、分离来源和分离部位的菌株具有相关性。 结论 串联重复序列位点分型技术具有简便、快速、特异、可比性、可重复性和高鉴别力等优点,适合嗜麦芽窄食单胞菌的分子流行病学研究。     

Diagnosis of trisomy 21 in fetal nucleated erythrocytes from maternal blood by use of short tandem repeat sequences

BACKGROUND: The purpose of this study was to determine whether aneuploid fetal nucleated erythrocytes (NRBCs) could be detected in maternal blood through the use of fluorescent PCR amplification with polymorphic short tandem repeat (STR) markers as an alternative or complementary method to analysis by fluorescent in situ hybridization (FISH). METHODS: Peripheral blood samples were obtained from women who had just undergone termination of pregnancy because of fetal trisomy 21 (three cases, 47,XY,+21; four cases, 47,XX,+21). Candidate fetal cells were isolated by flow-sorting by antibodies to the gamma chain of fetal hemoglobin and Hoechst 33342. FISH analysis was performed by the use of chromosome-specific probes for X, Y, and 21. Fetal NRBCs, as defined by the presence of gamma staining, characteristic morphology, and three chromosome 21 signals, along with maternal leukocytes, defined as gamma negative and two chromosome 21 signals, were micromanipulated separately and subjected to fluorescent PCR amplification of chromosome 21 STR markers (D21S11, D21S1411, and/or D21S1412). RESULTS: In five of seven cases analyzed, fetal NRBCs were aneuploid, as determined by the presence of triallelic or diallelic peaks of chromosome 21 sequences when compared with sequences from the maternal leukocytes. CONCLUSIONS: Fluorescent PCR amplification of STRs can detect fetal aneuploidy and may be useful in the setting of poor hybridization efficiency with FISH analysis. These results suggest that combined fetal aneuploidy and single-gene diagnoses by the use of DNA microarrays may be feasible in the near future.     

中国宁夏回族人群15个人类短串联重复序列位点的遗传多态性研究

目的研究宁夏回族人群15个人类短串联重复序列位点(STR)D3S1358、THO1、D21S11、D18S51、vWA、CSF1PO、D8S1179、TPOX、FGA、D5S818、D13S317、D7S820、D16S539、D19S433、D2S1338的遗传多态性。方法通过STR复合扩增、基因扫描、基因分型调查了102名回族无关个体15个STR位点等位基因分布情况。结果15个STR位点均符合Hardy-W e inberg平衡,共检出150个STR等位基因,其频率分布在0.004 9~0.553 9之间,杂合度(H)为0.625 5~0.865 4,个体识别力(DP)为0.834 7~0.960 6,非父排除率(EPP)为0.381 2~0.720 4,多态信息含量(PIC)为0.572 2~0.845 6。结论此研究为进一步研究中国回族STR遗传结构奠定了基础,在人类学、法医学等领域也有重要的应用价值。     

中国宁夏回族人群15个人类短串联重复序列位点的遗传多态性研究

目的研究宁夏回族人群15个人类短串联重复序列位点（STR）D3S1358、TH01、D21S11、D18S51、vWA、CSF1PO、D8S1179、TPOX、FGA、D5S818、D13S317、D7S820、D16S539、D19S433、D2S1338的遗传多态性。方法通过STR复合扩增、基因扫描、基因分型调查了102名回族无关个体15个STR位点等位基因分布情况。结果15个STR位点均符合Hardy-Weinberg平衡，共检出150个STR等位基因，其频率分布在0．0049—0．5539之间，杂合度（H）为0．6255—0．8654，个体识别力（DP）为0．8347—0．9606，非父排除率（EPP）为0．3812-0．7204，多态信息含量（PIC）为0．5722—0．8456。结论此研究为进一步研究中国回族STR遗传结构奠定了基础，在人类学、法医学等领域也有重要的应用价值。     

Y染色体特异STR位点应用于无创伤性产前胎儿遗传信息的研究

目的 建立利用孕妇血清进行无创伤性产前胎儿分子遗传信息分析的方法。方法 采集53名11～36孕周的孕妇的血清,利用血清中胎儿DNA,采用”Y-PLEX 6”试剂盒,复合扩增DYS393、DYS19、DYS389 Ⅱ、DYS390、DYS391和DYS385等6个Y-STR位点,PCR产物经基因测序仪电泳检测,用相关软件分析Y-STR基因型。结果 ①29名分娩出生证实为男婴的孕妇,均检测出特异性Y-STR等位基因。检测的6个Y-STR位点,以DYS393位点的检出率最高(29/29);其次是DYS19位点,检出率为62.07％(18/29);再次是DYS390位点,检出率为34.48％(10/29);其余的DYS389 Ⅱ、DYS391和DYS385位点的检出率则较低。②24名分娩出生证实为女婴的孕妇,均未检测出特异性Y-STR等位基因。③根据DYS393位点是否检测出特异性等位基因,以及该基因波峰的高度和波峰面积值,鉴定胎儿性别的准确率达100％。④29名妊娠男婴的孕妇的血清样本,检测出的Y-STR等位基因与“丈夫”的相一致。结论 本研究建立的无创伤性Y-STR分子遗传分析方法,具有多态性丰富、灵敏度高、特异性强等特点,提高了无创伤性产前胎儿性别遗传鉴定的准确性,同时为解决妊娠男婴的亲子鉴定提供了理论依据,具有广泛的应用前景。     

Molecular surveillance of methicillin-resistant Staphylococcus aureus by multiple-locus variable number tandem repeat fingerprinting (formerly multiple-locus variable number tandem repeat analysis) and spa typing in a hierarchic approach

In this study, clonal relatedness of 202 Staphylococcus aureus (mostly methicillin-resistant) isolates recovered in 29 Polish hospitals was investigated by multiple-locus variable number tandem repeat fingerprinting (MLVF) and spa typing. Our analysis yielded 69 MLVF patterns and 34 spa types. Almost all isolates (97.4%) identical by MLVF were also indistinguishable by spa typing. Therefore, the MLVF method can be a cheap and fast screen before spa typing. Moreover, results obtained by MLVF suggest a set of simple criteria for grouping of spa types. The proposed algorithm groups isolates into the same cluster when spa sequences differ by a single mutation event: i) a single deletion or insertion of repeat unit(s) at the X region of the protein A gene or ii) a single nucleotide polymorphism within a repeat sequence. The combined use of these 2 methods, MLVF in local laboratories and spa typing of selected isolates in reference centers, can improve the monitoring of hospital-to-hospital strain transmission events and public health interventions on a huge scale.     

短串联重复序列检测在异基因造血干细胞移植植入状态中的应用

背景:移植后造血干细胞植活的判断主要依赖于体内各种遗传标记,其在敏感性和有效性方面各不相同,故亟待建立一种鉴别力强、敏感性高、不受性别限制的检测方法。目的:观察异基因造血干细胞移植供受者移植前及受者移植后不同时间段的血样DNA短串联重复序列遗传位点检测情况。设计:观察测量实验。单位:深圳市血液中心输血医学研究所免疫遗传重点实验室。对象:选择2004-02/2005-12在深圳市血液中心输血医学研究所免疫遗传实验室配型成功并进行造血干细胞移植的18对供受者血样,18例患者中,男10例,女8例,平均35岁。接受血缘关系供者移植6例,无关供者移植12例。所有受试对象均对检测项目知情同意。方法:采用荧光标记复合扩增短串联重复序列(STR)检测技术,对18例进行造血干细胞移植的血液病患者移植后的系列血样及移植前供、受者的血样进行15个STR位点和1个性别位点的检测,找出供受者间的差异基因,观察移植后供者的STR基因在受者体内的植入情况及变化过程,找出最早检测到供者STR基因的时间及完全嵌合体最早出现的时间。主要观察指标:①观察移植前供受者差异基因。②供者STR基因及完全嵌合体最早出现时间。结果:供受者18对均进入结果分析。①供受者中能区分出彼此差别的平均STR差异位点数为12.4(8～15)个。②患者移植后最早可检测到供者STR基因的平均时间为8(5～14)d,由受者型向完全供者型转化的平均时间为14(9～23)d。植入状态由供受者嵌合型转为完全供者嵌合型。结论:荧光标记复合扩增STR检测方法可精确地描述异基因造血干细胞移植植入状态及其演变过程,可为临床提供一个准确、可靠的实验依据。     

Feasibility of a cost-effective approach to evaluate short tandem repeat markers suitable for chimerism follow-up.

BACKGROUND: Precise chimerism monitoring is important for the prediction of the success of allogeneic bone marrow transplantation (BMT). Most of the current procedures employed for chimerism follow-up with short tandem repeat (STR) markers are either time-consuming, labor-intensive, or use expensive assays, making it burdensome to perform large-scale studies of transplanted patients. AIM: To set-up a simple nonradioactive method to investigate a set of STR markers that could be used in the evaluation of chimerism status after allogeneic BMT. METHOD: Six dinucleotide STRs (D2S123, D5S107, CRTL1, D7S500, D11S1356, and TP53) were analyzed by touchdown (TD)-PCR followed by medium size non-denaturing polyacrylamide gel electrophoresis and silver staining. The sensitivity of the approach was evaluated by dilution competition assays. Peripheral blood samples were taken from a group of 50 healthy Argentinean donors, two transplanted patients, and their respective bone marrow donors. Buccal mucosa samples were also obtained from the BMT recipients. RESULTS: Four markers, D2S123, D7S500, D11S1356, and TP53, presented the highest heterozygosities (0.67-0.88) under our experimental system. A sensitivity of 0.8-1.6% for chimerism detection was consistently found for the different STR. The usefulness of these STR in chimerism analysis was illustrated with the screening of related siblings analyzing two transplanted patients with persistent mixed chimerism, which were previously studied by fluorescence in situ hybridization (FISH). Similar proportions of mixed chimerism were obtained with STR analysis compared with those estimated by FISH. DISCUSSION: To our knowledge, this was the first study of mixed chimerism using TD-PCR to achieve a highly specific STR amplification. This approach allows simple and accurate chimerism quantification because it avoids slippage of Taq polymerase on repeat stretches and prevents the differential amplification of the shorter allele. STR heterozygosities and the high level of sensitivity of this method demonstrated that this approach is not only very informative in this population, but is also rapid (taking less than 14 hours) and cost-efficient. CONCLUSION: The data confirms that this method is a useful tool applicable to routine large-scale STR genotyping and mixed chimerism analysis in low-complexity laboratories worldwide.     

四川汉族人群15个短串联重复序列基因座遗传多态性分析

目的获得15个短串联重复(short tandem repeat,STR)基因座在四川汉族人群中的群体遗传学数据。方法654份血样采自四川地区无血缘关系的汉族个体。Chelex法提取DNA,PCR复合扩增,自动基因分析仪电泳,收集电泳结果数据,基因扫描分析软件计算扩增产物片段相对大小,基因分型软件进行样本基因型分型。结果全部样本的每个STR基因座都获得了清晰的基因型分型结果。15个STR基因座的基因型分布符合Hardy-Weinberg平衡。15个STR基因座的杂合度介于0.6101—0.8654之间。累计非父排除率和累计个人识别率为0.999998766和>0.999999999。结论经1次扩增电泳可获得15个STR基因座的基因型分型结果,累计非父排除率和累计个人识别率较高,适合作为四川汉族人群的遗传标记,用于人类学、疾病连锁分析、法医学亲权鉴定和个体识别等领域的研究。     

Diagnosis of transfusion-associated graft-vs.-host disease: the importance of short tandem repeat analysis

Transfusion-associated graft-vs.-host disease (TA-GvHD) can occur following transfusion of blood products containing immunocompetent lymphocytes, usually from HLA homozygous donors, into immunocompromised patients sharing one HLA haplotype with the donor. The diagnosis of TA-GvHD may be delayed due to the initial nonspecific clinical features involved. Investigations to detect the presence of donor-derived cells in the blood and/or affected tissues of the recipient are essential to confirm the diagnosis. We report the investigation of suspected TA-GvHD using short tandem repeat (STR) analysis, to detect the presence of donor cells (chimerism), in an immunocompetent patient admitted for coronary artery bypass surgery. Peripheral blood and skin biopsies (from affected and nonaffected sites) from the patient and peripheral blood samples from the implicated donors were taken for HLA typing and STR analysis. STR analysis revealed the presence of donor material in the patient's peripheral blood sample and in DNA extracted from the affected skin biopsy but not the unaffected biopsy, suggesting lymphocytes from this donor were responsible for the development of TA-GvHD. Furthermore, HLA typing results supported the diagnosis of TA-GvHD. These data demonstrate the use of STR and HLA analysis as effective tools in the diagnosis of TA-GvHD.     

中国辽宁锡伯族群体3个短串联重复位点的遗传多态性分析

背景：对常染色体STR基因座的多态性的研究可为法医学亲权鉴定提供基础数据。目的：探索辽宁锡伯族D16S539,THO1,D13S3173个常染色体基因座的遗传多态性,建立锡伯族群体的遗传学基础数据。方法：采集辽宁省沈阳市新城子区黄家乡锡伯族中小学的150名中小学生口腔黏膜细胞,Chelex100法提取DNA,进行荧光标记PCR扩增,产物在Li-COR4300基因分析仪上进行电泳,E-seq分析软件计算扩增产物片段相对大小,进行基因型分型。调查辽宁地区锡伯族群体3个STR基因座等位基因频率,进行遗传多态性分析。结果与结论：辽宁锡伯族群体中3个STR基因座具有遗传多态性,其基因型分布符合Hardy-Weinberg平衡定律。辽宁锡伯族群体中3个STR基因座的杂合度分布在0.769~0.810;个人识别力分布在0.824~0.929,累积个人识别能力为0.999;多态信息量分布在0.650~0.790;非父排除率分布在0.565~0.790,累积非父排除率为0.979。说明辽宁锡伯族群体3个常染色体STR基因座有较高的非父排除率和个体识别能力,可为法医学亲子鉴定和个体识别及移植配型等遗传学研究提供依据。     

中国辽宁锡伯族群体3个短串联重复位点的遗传多态性分析

背景:对常染色体STR基因座的多态性的研究可为法医学亲权鉴定提供基础数据.目的:探索辽宁锡伯族D16S539,THO1,D13S317 3个常染色体基因座的遗传多态性,建立锡伯族群体的遗传学基础数据.方法:采集辽宁省沈阳市新城子区黄家乡锡伯族中小学的150名中小学生口腔黏膜细胞,Chelex 100法提取DNA,进行荧光标记PCR扩增,产物在Li-COR 4300基因分析仪上进行电泳,E-seq分析软件计算扩增产物片段相对大小,进行基因型分型.调查辽宁地区锡伯族群体 3个 STR基因座等位基因频率,进行遗传多态性分析.结果与结论:辽宁锡伯族群体中3个STR基因座具有遗传多态性,其基因型分布符合Hardy-Weinberg平衡定律.辽宁锡伯族群体中3个STR基因座的杂合度分布在0.769~0.810;个人识别力分布在0.824~0.929,累积个人识别能力为0.999;多态信息量分布在 0.650~0.790;非父排除率分布在0.565~0.790,累积非父排除率为0.979.说明辽宁锡伯族群体3个常染色体STR基因座有较高的非父排除率和个体识别能力,可为法医学亲子鉴定和个体识别及移植配型等遗传学研究提供依据.     

吉林地区STR系统6位点复合扩增的频率分布

目的分析吉林地区汉族人群短串联重复序列(STR)3个双基因座即6个STR位点的遗传多态性分布,获得相应多态位点的群体遗传学数据.方法采集103份吉林地区汉族无血缘关系的个体血样,提取DNA,经聚合酶链反应(PCR)分3组进行复合扩增(Ⅰ:TPOX CSF1PO,Ⅱ:D3S1358 D13S317,Ⅲ:D5S818 D19S400),非变性聚丙烯酰胺凝胶电泳(PAGE)分离后,银染显色分析.统计各等位基因的基因频率,分别计算各基因座的杂合度(H)、个人识别能力(DP)、非父排除率(PE)和多态性信息含量(PIC).结果得到6个基因座各等位基因的基因频率,经x2检验,基因型频率分布符合Hardy-Weinberg平衡,具有较高的杂合度和多态性信息含量.上述6个基因座的杂合度为0.7511～0.8376,个人识别能力为0.8273～0.9247,非父排除率为0.5167～0.6718,多态性信息含量为0.7100～0.8195.结论此STR 3个双基因座具有高度多态性,是较理想的遗传标志,所得到的等位基因频率等数据可为法医学个人识别、亲子鉴定及群体遗传学研究提供依据.