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1.
U. F. Gorskaya T. A. Danilova V. G. Nesterenko 《Bulletin of experimental biology and medicine》2009,147(1):73-76
The counts of stromal precursor cells in bone marrow transplants obtained from animals 2 months after their immunization with
killed type 5 group A streptococcus vaccine drop almost 3-fold in comparison with transplants from normal donors. Six months
after donor immunization, the count of stromal precursor cells in the transplants reaches the normal level. The count of stromal
precursor cells in bone marrow transplants from normal mice transplanted to recipients 6 months after their immunization with
killed streptococcus vaccine also virtually did not change in comparison with the counts in bone marrow transplants from normal
donors transplanted to normal recipients. The weight and size of bone capsules of 6.5-month bone marrow transplants in intact
recipients after transplantation from donors immunized 2 months before with killed type 5 group A streptococcus vaccine was
3-fold lower than in bone marrow transplants collected from intact donors. The content of stromal precursor cells in the femoral
bone marrow of animals immunized with killed streptococcus vaccine was 2.5 time higher in comparison with the parameter in
the femoral bone marrow of normal mice even 8 months after immunization. The results indicate a significant long-acting effect
of streptococcal antigens on the bone marrow stromal tissue, specifically, on its osteogenesis potential.
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 1, pp. 78-81, January, 2009 相似文献
2.
Gorskaya YF Danilova TA Lebedinskaya OV Lunin VG Grabko VI Sharapova NE Nesterenko VG 《Bulletin of experimental biology and medicine》2011,151(5):607-611
Immunization of CBA mice with killed group A streptococcus (type 5) vaccine changed the counts of stromal precursor cells
(CFC-F) in bone marrow transplants at different donorrecipient combinations (normal, N, or immune, I). CFC-F counts in bone
marrow transplants from normal mice transplanted to immunized animals decreased 4-6-fold depending on the transplant age in
comparison with similar transplants in normal recipients. The percentage of CFC-F colonies with alkaline phosphatase (osteogenesis
marker) activity decreased more than 2-fold. Similarly, the count of CFC-F in the transplants was 2-fold lower during delayed
(7 months) period after bone marrow transplantation from immunized donors (8–12 days after the end of immunization) to intact
recipients, while 2 months after transplantation it was 3-fold lower. The mean optical density of the bone capsule in preparations
stained for glycogen and alkaline phosphatase was 1.5-3 times lower in the N → I and I → N experiments in comparison with
the control (N → N). On the other hand, CFC-F count in the femoral bone marrow of immunized animals was significantly (3.5-2.5
times) higher during the period from 8 days to 8 months after the end of immunization compared to CFC-F count in the femoral
bone marrow of intact mice. These results attest to a significant prolonged effect of streptococcal antigens on the bone marrow
stromal tissue. These data also indicate that not all CFC-F, the counts of which increased in response to antigens, are responsible
for transplantability of the stromal tissue in heterotopic transplantation. Immunization by streptococcal antigens seemed
to suppress transplantability and osteogenic activity of stromal stem cells. The efficiency of CFC-F cloning in mouse bone
marrow cultures increased significantly (2-3-fold) in the presence of sera from immune mice. The levels of TNF-α and IFN-γ
were low in this serum (2.7 and 6 times lower, respectively) in comparison with normal serum. Presumably, the effects of streptococcal
antigens on stromal tissue were mediated through serum cytokines. 相似文献
3.
Gorskaya YF Danilova TA Lebedinskaya OV Nesterenko VG 《Bulletin of experimental biology and medicine》2005,140(1):66-69
The count of stromal precursor cells in bone marrow transplants from CBA mice, transplanted to animals immunized with killed
type 5 group A streptococcus vaccine, decreased 4.5–6.5 times (depending on the transplant age) in comparison with the grafts
transplanted to normal recipients. The counts of stromal precursor cells in 1.5–3-month bone marrow transplants from animals
immunized with killed streptococcal vaccine transplanted to normal mice were virtually the same, while in 7-month transplants
they decreased 2-fold in comparison with their counts in bone marrow transplants from normal CBA mice transplanted to normal
animals. The content of stromal precursor cells in the femoral bone marrow of animals immunized with killed streptococcal
vaccine was appreciably (3.5 times) higher than in the bone marrow of normal mice. The results attest to an appreciable effect
of streptococcal antigens on the bone marrow stromal tissue and suggest that not all stromal precursor cells, whose count
increases after injection of antigens, are responsible for transplantability of the stromal tissue in case of its heterotopic
transplantation.
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Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 140, No. 7, pp. 77–80, July, 2005 相似文献
4.
Gorskaya YF Kuralesova AI Shuklina EY Nesterenko VG 《Bulletin of experimental biology and medicine》2002,134(2):148-150
Efficiency of colony formation of stromal precursor cells in cultured bone marrow transplants from old (24 month) CBA mice implanted to young (2-month-old) mice almost 3-fold surpassed that in cultured transplants implanted to old recipients. The content of nucleated cells in bone marrow transplants from senescence accelerated mice SAMP increased more than 2-fold, if SAMR mice with normal aging rate were used as the recipients instead of SAMP mice. Bone marrow taken from old and young CBA mice endured the same number of transplantations if the recipient mice were of the same age (5 month). It was concluded that stromal tissue considerably changes with age and is under strict control of the body. 相似文献
5.
I. N. Nifontova N. V. Sats V. L. Surin D. A. Svinareva M. E. Gasparian N. J. Drize 《Bulletin of experimental biology and medicine》2008,145(1):133-136
We developed a method for gene transfer into mesenchymal stromal cells. Lentivirus vector containing green fluorescent protein
gene for labeling stromal and hemopoietic precursor cells was obtained using two plasmid sets from different sources. The
vector was injected into the femur of mice in vivo and added into culture medium for in vitro infection of the stromal sublayer of long-term bone marrow culture. From 25 to 80% hemopoietic stem cells forming colonies
in the spleen were infected with lentivirus vector in vivo and in vitro. Fibroblast colony-forming cells from the femoral bones of mice injected with the lentivirus vector carried no marker gene.
The marker gene was detected in differentiated descendants from mesenchymal stem cells (bone cavity cells from the focus of
ectopic hemopoiesis formed after implantation of the femoral bone marrow cylinder infected with lentivirus vector under the
renal capsule of syngeneic recipient). In in vitro experiments, the marker gene was detected in sublayers of long-term bone marrow cultures infected after preliminary 28-week
culturing, when hemopoiesis was completely exhausted. The efficiency of infection of stromal precursor cells depended on the
source of lentivirus. The possibility of transfering the target gene into hemopoietic precursor cells in vivo is demonstrated. Stromal precursor cells can incorporate the provirus in vivo and in vitro, but conditions and infection system for effective infection should be thoroughly selected.
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Translated from Kletochnye Tehnologii v Biologii i Meditsine, No. 1, pp. 25–28, January, 2007 相似文献
6.
Previously, we have reported that although unperturbed granulocyte colony-stimulating factor (GCSF)-deficient (G-CSF-/-) mice are neutropenic, when challenged with Candida albicans, they develop a profound neutrophilia. In an attempt to understand the basis of Candida-induced neutrophilia in G-CSF-deficient mice, we have modified the Dexter bone marrow culture system to produce an in vitro model that mimics emergency granulopoiesis in vivo. In this model, stromal cultures are overlaid with bone marrow cells in the presence or absence of heat-inactivated (HI) Candida. Irrespective of the genotype of mice used as a source of bone marrow-derived stromal cells, stimulation of these cultures with HI Candida led to a significantly greater recovery of cells compared to unstimulated stromal cultures. In addition, there was a marked increase in the number of colony-forming units granulocyte-macrophage (CFU-GM), as well as in the percentage of granulocytes in the population of nonadherent cells recovered from HI Candida-stimulated cultures. The conditioned medium generated from stromal cultures derived from either wild-type or G-CSF-/- mice exposed to HI Candida, when applied to bone marrow cells in a soft agar clonogenic assay stimulated M-, GM-, and G- type colonies. Interleukin-3 (IL-3) and GM-CSF could not be detected in the conditioned medium from either HI Candida stimulated or unstimulated stromal cultures. However, IL-6 was detected in the conditioned media from both wild-type and G-CSF-/- stromal cultures. Addition of anti-IL-6 antibody significantly impaired granulopoiesis in unstimulated and HI Candida-stimulated, wild type, and G-CSF-/- stromal cultures. Conditioned medium generated from G-CSF/IL-6-deficient stromal cells had the capacity to stimulate bone marrow cells to form colonies comprised of granulocytes and macrophages in soft agar clonogenic assay. This study demonstrates that stromal cells can be stimulated with HI Candida and gives an insight into Candida mediated granulopoiesis. 相似文献
7.
L. P. Gerasimova T. E. Manakova N. V. Tsvetaeva E. A. Lukina A. S. Kozlovskaya 《Bulletin of experimental biology and medicine》1999,127(1):10-13
Using clonal methods for assessment of hemopoietic and stromal cells and long-term bone marrow cell cultures, we have demonstrated
heterogeneity of myelodysplastic syndrome. Low content of stromal precursor cells in native bone marrow, peculiarities in
the formation of the stromal layer and its hemopoiesis-stimulating capacity in long-term cultures, and altered properties
of stromal precursor cells in long-term cultures indicate defect in the stromal microenvironment in myelodysplastic syndrome.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 1, pp. 14–18, January, 1999 相似文献
8.
AA Churin LA Ermolaeva TY Dubskaya TI Fomina TV Vetoshkina AV Artamonov AA Bekarev PG Madonov 《Bulletin of experimental biology and medicine》2011,151(2):210-214
The study was carried out on CBA mice using the method of heterotopic transplantation. A fragment of the femoral bone marrow
(1/2) or spleen (1/5 of the organ) was transplanted under the renal capsule of a recipient. The following donor-recipient cross-transplantation
variants were studied: young → young (Y → Y), young → old (Y → O), old → old (O → O), and old → young (O → Y). Cell suspensions
were prepared from 2-month transplants inoculated in monolayer cultures and the cloning effi ciency (ECF-F) of stromal precursor
cells (CFC-F) was evaluated. The bone marrow transplant ECF-F and the count of CFC-F in the O → O group were 8-fold lower
than in the Y → Y group. In the O → Y group, ECF-F was 3-fold higher than in the O → O group, but by 2.5 times lower than
in the Y → Y group. ECF-F in Y → O group was 2-fold lower than in Y → Y group. The ECF-F and CFC-F count in spleen transplants
in the O → O group were 4- and 6-fold lower, respectively, than in Y → Y group. However, in O → Y group ECF-F was 7-fold higher
than in O → O group and higher than even in Y → Y group. The weight of induced ectopic bone tissue after transplantation of
the osteoinductor (fragments of the allogenic urinary bladder mucosa) was 2-fold lower in the O → O vs. Y → Y group. However, comparison of the ectopic bone tissue weights in different experimental groups showed that osteoinductor
activity of the bladder epithelium did not decrease, but increased 3-fold with age (O → Y:Y → Y). A 5-fold reduction of this
proportion in groups where the osteoinductor was transplanted from old donors to old and young recipients (O → Y:O → O) could
be attributed to age-specifi c reduction of the count of inducible osteogenic precursor cells (IOPC). The data in general
suggest that age-specifi c reduction of the stromal precursor count and functional activity could be caused by the true reduction
(exhaustion) of cell pool (bone marrow CFC-F; presumably, IOPC) and by the regulatory effects of the organism (bone marrow
and splenic CFC-F, IOPC). These data seem to be signifi cant for understanding of the role of osteogenic stromal precursor
cells in the development of age-associated bone tissue defects, for example, senile osteoporosis. 相似文献
9.
Functional disturbances in hemopoietic microenvironment in various forms of myelodysplastic syndrome
T. E. Manakova L. P. Gerasimova N. V. Tsvetaeva K. S. Momotyuk 《Bulletin of experimental biology and medicine》2000,130(3):832-834
We studied the ability of stromal sublayer of long-term bone marrow cultures and peripheral blood macrophages from patients
with various forms of myelodysplastic syndrome to maintain the growth of normal granulocyte-macrophage colony-forming units
in mixed cultures. There were changes in the hemopoietic microenvironment in these patients: decreased cellularity of the
bone marrow and impaired formation of sublayers in long-term bone marrow cultures, production of growth factors, maintaining
the growth of normal granulocyte-macrophage precursors by stromal cells. Dysfunction of macrophages in the stromal microenvironment
was probably related to the presence of pathological macrophages.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 255–258, September, 2000 相似文献
10.
Functional disturbances in hemopoietic microenvironment in various forms of myelodysplastic syndrome
Manakova T Gerasimova L Tsvetaeva N Momotyuk K 《Bulletin of experimental biology and medicine》2000,130(9):832-834
We studied the ability of stromal sublayer of long-term bone marrow cultures and peripheral blood macrophages from patients
with various forms of myelodysplastic syndrome to maintain the growth of normal granulocyte-macrophage colony-forming units
in mixed cultures. There were changes in the hemopoietic microenvironment in these patients: decreased cellularity of the
bone marrow and impaired formation of sublayers in long-term bone marrow cultures, production of growth factors, maintaining
the growth of normal granulocyte-macrophage precursors by stromal cells. Dysfunction of macrophages in the stromal microenvironment
was probably related to the presence of pathological macrophages.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 255–258, September, 2000 相似文献
11.
The proliferative activity of precursors of bone marrow stromal cells forming colonies (clones of fibroblasts) in monolayer cultures was studied by the thymidine-suicide method in vitro. Clonogenic cells of native bone marrow were shown not to be inhibited by thymidine-H3 with high specific activity, i.e., they virtually do not proliferate in vivo. Having started to proliferate 24 h after explantation, they then give rise to colonies of fibroblasts. In 6–14-day bone marrow cultures 39±4% of clonogenic cells die under the influence of thymidine-H3. Hence it follows that, unlike precursor cells in native bone marrow, clonogenic cells in primary cultures proliferate actively.N. F. Gamaleya Institute of Epidemiology and Microbiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR O. V. Baroyan.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 81, No. 1, pp. 55–57, January, 1976. 相似文献
12.
UF Gorskaya TA Danilova MV Mezentzeva MM Shapoval VG Nesterenko 《Bulletin of experimental biology and medicine》2012,153(1):64-67
Injection of polyvinylpyrrolidone (synthetic type 2 T-independent antigen) stimulated the efficiency of clone-forming efficiency
and the content of stromal precursor cells in CBA mice in the femoral bone marrow (almost 3-fold) and in the spleen (by 1.7
times) with the peak within 24 h and normalization by day 3 after immunization. The expression of IL-6, IL-8, and TNF-α genes
in bone marrow and spleen cultures from immunized animals appeared on day 1 and disappeared on day 3. Hence, stimulation of
stromal tissue in response to polyvinylpyrrolidone immunization was significantly less pronounced in comparison with immunization
with S. typhimurium antigens. The counts of stromal precursor cells in these organs did not increase in CBA/N mice not responding to polyvinylpyrrolidone
because they had no xidmutation in Brutton’s tyrosine kinase (Btk) gene, and the proinflammatory cytokine genes expression
in primary cultures derived from these animals did not increase either. These data indicated that the degree of stromal tissue
stimulation in immunized mice correlated with the immune response intensity. This indicated a close relationship between the
stromal tissue and immune system. Stromal tissue seemed to be stimulated not only and not so much through the stromal cell
Toll-like receptors, but mainly through interactions of immunocompetent and stromal cells, the former presumably playing the
leading role in this process. 相似文献
13.
Nikitina VA Osipova EY Katosova LD Rumyantsev SA Skorobogatova EV Shamanskaya TV Bochkov NP 《Bulletin of experimental biology and medicine》2011,150(5):627-631
Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells
were studied. Despite the reduction of proliferative activity by passages 11–12, the cells retained the characteristic immunophenotype.
The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal
stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results
indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during
in vitro culturing. 相似文献
14.
目的:以雌性小鼠骨髓移植给雄性小鼠的方法,通过检测雄性小鼠血细胞的Y染色体来明确内源性骨髓细胞的残存状态。方法:将雌性或雄性C57BL/6小鼠作为受体,实验组用[137Cs]照射,6 h后每只经尾静脉注射供体小鼠骨髓细胞1×107。统计骨髓移植后14 d动物的存活率,并通过眶静脉采血观察外周血白细胞数量的变化,检测受体雄性小鼠体内Y染色体基因水平的变化以明确骨髓移植的效果。结果:分别用1 000、950和900 rad的照射剂量对受体小鼠进行照射后将供体小鼠的骨髓移植到受体小鼠体内,1 000和950 rad剂量时雌性受体小鼠可迅速恢复造血功能,而雄性受体小鼠则仅有48%的存活率。900 rad照射剂量骨髓移植后,雄性受体小鼠迅速恢复了造血功能,13 d后外周血白细胞计数基本恢复正常。移植后的雄性受体小鼠在5周内已检测不到外周血细胞Y染色体基因,表明雄性受体小鼠的骨髓被完全破坏,雌性供体小鼠的骨髓可完全替代受体雄性小鼠的骨髓并且发挥造血功能。结论: 在照射剂量900 rad照射后,雄性小鼠可以作为骨髓移植受体,为将来应用雄性小鼠作为骨髓移植受体动物开展有关心血管疾病的研究奠定了实验基础。 相似文献
15.
Kolesnikova AI Pavlov VV Konoplyannikov AG Lepekhina LA Kal'sina SSh Danilova MA Domracheva EV Vorob'ev AI 《Bulletin of experimental biology and medicine》2002,134(2):181-186
Growth characteristics of human hemopoietic cells in erythremia and chronic myeloid leukemia were studied using agar cultures with and without hemopoietic growth factors. Agar cultures, similarly to cultures on other semisolid media (plasma clot, methylcellulose) can be used for early differential diagnosis of polycythemia vera (erythremia) and secondary erythrocytosis: erythremia, but not erythrocytosis, is characterized by spontaneous (erythropoietin-independent) formation of colonies from erythrocyte precursor cells. Spontaneous colony formation from granulocyte-macrophage precursor cells can serve as an important test for early diagnosis of chronic myeloid leukemia. The study of colony formation from granulocyte-macrophage precursors and of the capacity of bone marrow cells to form colonies from hemopoietic stromal precursor cells revealed new characteristics of the studied myeloproliferative diseases. Presumably, spontaneous colony formation from erythrocytic and myeloid precursors should be regarded as a sign of tumor transformation of the studied hemopoietic cells. 相似文献
16.
A. Ya. Fridenshtein Yu. F. Gorskaya N. V. Latsinik E. Yu. Shuklina L. A. Fonina S. A. Gur'yanov 《Bulletin of experimental biology and medicine》1999,127(2):197-199
Fibroblast growth factors aFGF, bFGF, IGF-I, IGF-II, TGF-β, EGF, and PDGF did not stimulate the formation of stromal fibroblast
colonies (CFC-F-colonies) in cultured murine adhesive bone marrow cells. It means that colony-stimulating activity of the
bone marrow feeder cells with respect to the formation of stromal fibroblast colonies does not depend on the known growth
factors. A scheme and conditions of culturing are developed for preparing conditioned media from bone marrow cell cultures
which can replace the CFC-F colonystimulating activity of the bone marrow feeder cellsin vitro. Primary separation of conditioned media by ultrafiltration reveals that only the fraction with molecular weight of more
than 65 kD exhibits CFC-F colony-stimulating activity.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 2, pp. 218–220, February, 1999 相似文献
17.
U. F. Gorskaya O. U. Tretyakov A. P. Suslov V. G. Nesterenko 《Bulletin of experimental biology and medicine》2008,146(6):759-762
The content of stromal precursor cells in the bone marrow of mice decreased 2–5.7 times 24 h after injection of macrophage
migration inhibition factor in doses of 0.1–50 ng/kg, this reduction depending on the dose of inhibition factor. The content
of precursor cells in the bone marrow of mice increased 2-fold 24 h after injection of S. typhimurium bacterial mass. One day after injection of S. typhimurium bacterial mass, the count of precursor cells in mouse spleen was 7-fold higher than 24 h after injection of macrophage migration
inhibition factor. The efficiency of cloning of mouse bone marrow stromal precursor cells in vitro was suppressed 1.7–2.8 times in the presence of macrophage migration inhibition factor in doses of 0.1 to 50 ng/ml culture
medium. The effect of cloning inhibition was preserved, if macrophage migration inhibition factor was added to the culture
medium after 2 days of bone marrow cell culturing. In general, macrophage migration inhibition factor inhibits stromal precursor
cells in vivo and in vitro. The data also indicate that macrophage migration inhibition factor is not responsible for rapid and sharp increase in the
count of stromal precursor cells after immunization of animals.
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 12, pp. 667–670, December, 2008 相似文献
18.
A. A. Ivanov-Smolenskii Yu. F. Gorskaya A. I. Kuralesova N. V. Latsinik 《Bulletin of experimental biology and medicine》1976,82(4):1588-1590
The strain of origin of fibroblasts growing in monolayer bone marrow cultures of semisyngeneic heterotopic grafts and radiochimeras was investigated by the indirect immunofluorescence method using an isoantiserum. All fibroblasts from bone marrow of complete radiochimeras were shown to be of recipient origin whereas histocyte—macrophages in the same cultures were of donor origin. All fibroblasts in bone marrow cultures of heterotopic grafts belonged to the original donor, whereas 80% of the histiocytes were of recipient origin. This proves directly that the stromal mechanocytes and hematopoietic cells, and also the macrophages, belonged to different cell lines.Laboratory of Immunomorphology, N. F. Gamaleya Institute of Epidemiology and Microbiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR O. V. Barovan.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 10, pp. 1270–1271, October, 1976. 相似文献
19.
Radyukhina NV Rutkevich PN Aref'yeva TI Gurskaya TKh Rybalkin IN Shevelyov AY Slinkin MA Vlasik TN Il'yinskaya OP Tararak EM 《Bulletin of experimental biology and medicine》2007,143(6):723-726
We developed a technology of labeling bone marrow precursor cells with the Lin—c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective
transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 143, No. 6, pp. 667–671, June, 2007 相似文献
20.
Fan TX Hisha H Jin TN Yu CZ Lian ZX Guo SB Cui YZ Feng B Yang GX Li Q Ikehara S 《Stem cells (Dayton, Ohio)》2001,19(2):144-150
We examined the importance of the coadministration of bone marrow (BM) stromal cells with BM cells via the portal vein. A significant increase in the number of day-14 colony-forming unit-spleen (CFU-S) was observed in the recipient mice injected with hemopoietic stem cells (HSCs) along with donor BM stromal cells obtained after three to four weeks of culture. Histological examination revealed that hematopoietic colonies composed of both donor hemopoietic cells and stromal cells coexist in the liver of these mice. However, when donor HSCs plus BM stromal cells were administered i.v., neither the stimulatory effects on CFU-S formation nor the hemopoietic colonies in the recipient liver were observed. These findings suggest that the interaction of HSCs with stromal cells in the liver is the first crucial step for successful engraftment of allogeneic HSCs. It is likely that donor stromal cells and HSCs trapped in the liver migrate into the recipient BM and spleen, where they form CFU-BM and CFU-S, respectively. 相似文献