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1.
R. A. Bürger C.-D. Gerharz H. Rothe U.H. Engelmann R. Hohenfellner 《Urological research》1991,19(4):253-257
Summary A comparative study was undertaken in 81 rats to investigate a CO2 and Nd:YAG laser system for laserwelded anastomosis of the femoral vein. Conventionally sutured anastomoses (CMSA) served as controls. Laserwelded anastomosis (LAMA) was easier and could be performed 30% faster than CMSA. Postoperative investigations included patency tests, postmortem examinations and light and electron microscopy. Aneurysms were not seen. Foreign-body reaction was more pronounced in CMSA. Patency rates for CO2-LAMA and CMSA were equal, whereas Nd:YAG-LAMA resulted in significantly higher rate of early postoperative thrombosis (P<0.01). Because of its physical properties, the CO2-laser system seems to be better suited for laser welding of delicate structures such as the rat femoral vein. 相似文献
2.
Low power CO2 laser-assisted microvascular anastomosis (LAMA) was used for saphenous artery autotransplantation in 40 rabbits. Eighty end-to-end anastomoses were performed by three methods (conventional interrupted suture, stay suture + laser, laser without stay suture). The long term patency rate in the three types of anastomosis is 93%, 93%, and 100%, respectively. The time needed for the laser procedure is half to one-third of that required for the interrupted suture method. The tensile strength of the bonding site can withstand up to 250 mm Hg of arterial pressure. We have demonstrated that the CO2 laser can be used in microvascular anastomosis with or without stay sutures. Aneurysm formation is a potential risk of LAMA. Accurate coaptation of the transected vessel, precise control of laser energy, and minimal area exposed to the laser beam are the key points for further improving anastomotic quality. 相似文献
3.
In order to compare the carbon dioxide laser-assisted microvascular anastomosis (CO2 LAMA) with conventional microvascular sutured anastomosis (CMSA), 40 microarterial anastomoses were performed in Wistar albino rats. At different time intervals from zero to four weeks after the procedure, the anastomoses were examined under the scanning electron microscope (SEM) after resin corrosion cast (Mercox). CO2 LAMA was easier and less time-consuming than CMSA, with the same patency rate. Healing of the lumen surface was similar in both procedures, suggesting that CO2 LAMA can be reliably used in microvascular anastomosis. 相似文献
4.
Laser-assisted microsurgical anastomosis 总被引:1,自引:0,他引:1
A low power carbon dioxide laser was used to perform 212 end-to-end laser-assisted microvascular anastomoses (LAMA) of femoral arteries (mean diameter, 1.2 mm) in Sprague-Dawley rats. Eighty-two conventional microvascular suture anastomoses (CMSA) utilizing 10-0 monofilament interrupted sutures were done for comparison of techniques and wound healing. The mean duration of each anastomosis procedure was 16 minutes for the LAMA repairs, compared to an average of 27 minutes for the CMSA repairs (P less than 0.05). All anastomoses were patent at the completion of the procedure. Each laser-assisted anastomosis required an average of seven intermittent laser exposures of 0.1 to 0.3 seconds each with approximately 80 mW of CO2 (wavelength = 10.6 micron) radiation at a spot size of 150 micron. A patency rate of 95% was obtained on the LAMA vessels (202 of 212) compared to 96% for the CMSA repairs (79 of 82). A total of 14 aneurysms were noted in the LAMA group (7%) compared to 11 in the CMSA (13%). All aneurysms were in patent vessels. Histological analysis indicates that the progression of wound healing of LAMA and CMSA anastomoses follows similar paths chronologically and morphologically with increased scar tissue formation around the suture. Scanning electron microscopy confirms the comparable luminal healing of the LAMA and CMSA vessels, with complete reendothelialization occurring by 3 weeks postoperatively. The tensile strength of the LAMA repair, although low immediately after operation, is comparable to that of the intact artery at 21 days. These findings suggest that a low energy carbon dioxide microsurgical laser has potential beneficial clinical application for anastomosis of small vessels. 相似文献
5.
The aim of the present study was to demonstrate the usefulness of our newly produced diode laser device (an 830-nm output of 190 mW CW) for microvascular end-to-end anastomoses. In the device, diode laser energy is delivered to a contact ceramic tip with a 200-microns diameter output end. Its wavelength is 830 nm and its power 60 mW. This device is small (30 x 25 x 10 cm), lightweight (gross weight 3kg), and easy to manipulate. Compared with conventional microvascular suture anastomosis (CMSA), the time required for the laser-assisted vascular anastomoses (LAVA) was reduced by almost half. Anastomosed portions of both methods were examined 1, 2, 4, 12, and 24 weeks later for patency and aneurysm formation. The patency rate and incidence of aneurysmal formation were the same in both groups. Histological examination revealed that LAVA is superior to CMSA in the smoothness of the inner surface, less inflammatory reaction, and normal collagen and elastic reorientation. The tensile strength and bursting pressure measured immediately after anastomosis demonstrated no significant difference between the two groups. It was concluded that this diode laser contact method may be clinically useful for microvascular surgery. 相似文献
6.
Arterial reconstruction with vascular clips is safe and quicker than sutured repair. 总被引:2,自引:0,他引:2
E Pikoulis P Rhee T Nishibe A K Lepp?niemi N Fishback H V Hufnagel D C Wherry N M Rich 《Cardiovascular surgery (London, England)》1998,6(6):573-578
BACKGROUND: Non-penetrating, arcuate-legged vascular-closure staple clips made of titanium were initially developed for microvascular anastomoses with little experience of their use in larger vessels. The purpose of this study was to compare vascular-closure staple clips to sutured anastomoses in common iliac arteries in a porcine model. METHODS: In an experimental study, transected iliac arteries on both sides of 11 pigs were randomly assigned to end-to-end anastomosis performed with vascular-closure staple clips or interrupted 6-0 polypropylene sutures. Angiographic, macroscopic and microscopic results were assessed after 2 months. RESULTS: There was no significant difference in the patency rate, tensile strength of the anastomoses, vessel diameter at the repair site, intimal thickness or wall thickness of the arteries after either method of closure. The mean (s.d.) clamp time was 19.8 (6.1) minutes for clip repair, and 36.0 (6.9) seconds for suture repair (P < 0.001). The times required for the reconstruction of the anastomoses were 17.4 (6.1) and 35.5 (7.1) minutes, respectively (P < 0.001). CONCLUSIONS: Arterial anastomoses performed with vascular-closure staple clips are faster than sutured anastomoses, and result in comparable wound healing when assessed for patency, tensile strength, degree of narrowing and intimal reaction. 相似文献
7.
Summary In this study the effect of CO2 laser on spinal epidural fibrosis was examined in 24 guinea pigs which were divided into two groups. The first group was the control group, the second one the CO2 laser group.All animals had laminectomies at 3 levels. Re-exploration was performed three months after the laminectomy. In the second group the same procedure was performed but at the end of the re-exploration, CO2 laser irradiation of the epidural fibrotic tissue was done. The wounds in both groups were closed again. Four months later all animals were sacrificed, for verification and quantification of scar formation (postoperative fibrosis) light microscopic examinations and determination of hydroxyproline were done, using Bergman's spectrophotometric method. The differences which were observed between the two groups were statistically significant (U 134, p < 0.05).This study demonstrates the reducing effect of CO2 laser irradiation on epidural scar formation. The findings encourage its use as an alternative method of prevention of epidural fibrosis after spinal surgery. 相似文献
8.
Intestinal welding by means of low-power laser has been reported as an efficient method for intestinal anastomosis. We designed an experimental model in rats to investigate collagen and DNA concentrations in CO2 laser–welded anastomoses as compared with those in sutured anastomoses on the 4th, 7th, and 10th postoperative days. The results revealed that DNA, total collagen, and insoluble collagen concentrations were significantly lower in the lased anastomoses than in the sutured anastomoses on the 4th postanastomotic day. On the 7th and 10th postanasto-motic days, collagen concentrations increased in the laser-treated group attaining significantly higher levels than in the sutured group at that time. These findings are compatible with other studies demostrating that laser–welded intestinal anastomoses are more prone to dehiscence during the first 4 postanastomotic days, but become at least as effective as the sutured ones with passage of time. © 1994 Wiley-Liss, Inc. 相似文献
9.
P E Grubbs S Wang C Marini S Basu D M Rose J N Cunningham 《The Journal of surgical research》1988,45(1):112-119
Laser-assisted microvascular anastomoses (LAMA) are characterized by low early bursting strength and high aneurysm rates. The effects of fibrin glue on bursting strength (BS), patency, and aneurysm rate of LAMAs were compared to standard suture and laser anastomosis. Rat femoral arteries (0.9-1.1 mm) were anastomosed end-to-end by three methods: (1) conventionally with 8 to 10 interrupted 10-O nylon sutures; (2) 3 stay sutures and CO2 laser (spot size, 0.275 mm; pulse, 0.2 sec, 80 mW); and (3) cryoprecipitated fibrinogen, 35 mg/cc, crystallized thrombin, CaCl, 20 mg/cc, aprotinin (2000 kIU/cc) applied to weld site in conjunction with laser weld as in (2) above. Patency, aneurysms, and histology were evaluated at 3 weeks, and BS (mm Hg) was measured in six additional vessels at 1 and 24 hr. There was no statistically significant difference in patency rates. Both the suture and fibrin glue groups had significantly higher 1 and 24 hr bursting strengths (P less than 0.05) and significantly lower aneurysm rates (P less than 0.001) than standard laser. There was no significant difference in bursting strength between suture and fibrin glue groups. Histology in the fibrin glue group showed medial damage similar to the LAMA and calcification of aneurysmal vessels. Fibrin glue enhancement of LAMAs produces equal patency, higher early bursting strength, and fewer aneurysms at 3 weeks compared to conventional laser. 相似文献
10.
Technological advances have made CO2 laser-assisted microvascular anastomoses (LAMA) feasible. This study seeks to compare results of LAMA versus CSMA (conventional suture microsurgical anastomosis) in traumatized vessels. Using a rat model, femoral arteries and veins were either crushed and transected or divided by avulsion and then repaired by either LAMA or CSMA. LAMA resulted in higher patency rates than CSMA at early postoperative observation periods. With time, the patency rates improved in both groups and, by the end of the study, the patency rates were equivalent. These findings indicate that the laser technique may be a better option when working with traumatized vessels because of the critical nature of early patency rates. The improved results may be due to a reduction of suture material at the anastomotic site when using the laser technique. Some physiologic aspects of vessel thrombosis, recanalization, and the role of collateral circulation are discussed. 相似文献
11.
D F Cikrit M C Dalsing T S Weinstein K Palmer S G Lalka J L Unthank 《Lasers in surgery and medicine》1990,10(6):584-590
The milliwatt CO2 laser was used to perform end-to-end anastomoses in canine jugular veins. There was a high disruption rate (50%) in laser-welded veins (n = 10). Fibrin glue (n = 17), formed from human fresh-frozen plasma, enhanced the weld strength decreasing the disruption rate (18%), resulting in an 82% patency which nearly equaled the contralateral sutured vein patency (93%). The bursting strength was improved with fibrin glue. Transmural necrosis was present initially in all groups but extended for a longer distance in the vessel wall in laser-welded anastomoses. Sutured anastomoses exhibited a greater inflammatory response. In laser-welded anastomoses endothelial cells were not as confluent as in sutured anastomoses by six weeks. Carbon dioxide laser-welded end-to-end vein anastomoses appear to be impractical because they disrupt too easily. However, the addition of heterologous fibrin glue to the weld results in a reasonably strong anastomosis with histologic properties that may be beneficial in vein bypass grafts. 相似文献
12.
Jing Tang Guilhem Godlewski Simone Rouy Michel Dauzat Jean-Marie Juan FranOis Chambettaz Rene Salathe 《Lasers in surgery and medicine》1994,14(3):229-237
A series of direct carotid end-to-end laser anastomosis vs. direct manual suture was carried out on a series of 70 Wistar rats (mean weight 260 g). Both common carotids (0.8–1.2 mm) were sectioned and repaired. The left side (n = 70) was submitted to laser-assisted microvascular anastomosis (LAMA) performed by means of a diode laser device (wavelength 830 nm and power output 3 W in continuous wave) without chromophore. The right side (n = 70) underwent a control manual suture (CMA). The diode laser energy was delivered into a micromanipulator coupled to a Zeiss operating microscope with a focused spot of 300 μm in diameter. After placement of three 10.0 stitches for edge coaptation, the LAMA was achieved using laser shots (average 3) of 500 mW power, 4.5 s duration, and 700 W/cm2 irradiance each. The CMA was performed by means of six 10.0 stitches. The good vascular flow was confirmed by Doppler spectral analysis (n = 466) carried out from day 0 to day 90. Light and scanning electron microscopy (n = 82) showed that re-endothelialization after LAMA was gaining ground on day 3, whereas collagenous network developed in the media scar by day 10. In contrast, after CMA the arterial repair was delayed on day 20, inducing a media fibrotic scar. The patency rate was 93% in both anastomoses. The shorter operating time (13 min for LAMA vs. 22 min for CMA) and the noncontact laser technique are the main intraoperative advantages. The technical benefits of the diode laser are pointed out. © 1994 Wiley-Liss, Inc. 相似文献
13.
Thirty years of laser-assisted microvascular anastomosis (LAMA): what are the clinical perspectives?
Introduction
Since the first studies by Jain and Gorisch (1979), laser-assisted anastomoses have been steadily developed to a stage where clinical use is within reach. The laser-assisted vascular microanastomosis (LAMA) procedure is performed more quickly than conventional anastomosis, the surgically induced vessel damage is limited, and reduced bleeding after unclamping is observed.Material and methods
A Medline literature search, for the January 1979 to February 2010 period, was performed to review articles focusing on the LAMA technique.Results
The search yielded a total of 354 publications, of which 87 were relevant: 82 were animal series and five clinical studies. Microsurgical techniques and principal characteristics of LAMA in patients are the focus of the analysis. This study discusses the technological innovations and new orientations in laser welding.Conclusion
The first two clinical series using the 1.9-μm diode laser appear promising. Technical innovation will most likely lead to greater ease of use of the laser handpiece in the operating room. 相似文献14.
A Ruiz-Razura M Lan C E Hita Z Khan N H Hwang B E Cohen 《Journal of reconstructive microsurgery》1988,4(4):291-296
Iliac artery end-to-end anastomoses were performed in 42 Sprague-Dawley rats, divided into seven groups, to determine the welding effects of CO2 laser radiation in microvascular anastomoses. Conventional suture techniques were performed on right iliac arteries, and left iliac arteries were anastomosed with a laser-assisted technique. Bursting strength and diameters of the anastomotic sites were measured at different intervals (from one day to five weeks) post surgery. The anastomotic patency rate was 100 percent in both groups, and the aneurysm rate was only 2 percent in the laser group. Bursting strength was low at one and three days post surgery in both groups; then, it increased gradually until both groups could withstand higher than physiologic pressures. Results of high patency rates, low aneurysm formation, and the ability to withstand pressures higher than physiologic, suggest that the laser-assisted anastomotic technique can play an important role in microvascular surgery. 相似文献
15.
Laser assisted microvascular anastomosis (LAMA) was performed on rat femoral arteries anddd veins using a milliwatt CO2 laser. The histological appearance of the anastomoses was assessed immediately after the procedure and at regular intervals up to seven months. There was extensive thermal-induced transmural necrosis. The weld at the anastomosis was shown to comprise of fibrin, which later organized into fibrous tissue. Re-endothelialization occurred earlier in veins, and the endothelial nature of the surface cells was confirmed by factor VIII-related antigen positivity by the avidin-biotin peroxidase complex technique. Healing was complete by 9 weeks, but the vessel was not reconstituted to its normal appearance. At the site of the anastomosis 20% of the vessels had false aneurysms. 相似文献
16.
Laser-assisted arterial anastomoses can now be performed with satisfactory short-term patency. This study was undertaken to evaluate patency and aneurysm formation with a 1-year follow-up. A microscopically guided CO2 laser was used to anastomose 1.5 to 2.0 mm carotid arteries in 24 rabbits. Under X6 to X40 magnification, 60 to 70 mW were delivered with a spot size of approximately 0.32 mm. One carotid artery underwent laser anastomosis; the opposite served as a sutured control (10-0 nylon sutures). The 48 end-to-end anastomoses were evaluated for patency and aneurysm formation at 3, 6, and 12 months. Aneurysms were defined as a 1.5 times increase in diameter at the anastomotic site. The vessels underwent microscopic examination. All laser-assisted and sutured anastomoses were patent up to 1 year. At 3 months, one of eight sutured and one of eight laser anastomoses were aneurysmal; stenosis was noted in one laser anastomosis. At 6 months, one of eight laser and 0 of eight sutured anastomoses were aneurysmal. At 12 months, one of eight rabbits had died; of the remaining seven, three of seven laser and zero of seven sutured anastomoses were aneurysmal. In total, five of 23 (21.7%) aneurysms developed with the laser technique and one of 23 (4.3%) with the suture technique (p less than 0.05). Laser-assisted anastomoses are technically feasible, and patency at 1 year is equal to those performed with the suture technique. Aneurysm formation is a consistent problem that demands further investigation. 相似文献
17.
Sutured anastomoses of small vessels are considered difficult to learn. Mechanical anastomosis systems allow a more rapidly performed anastomosis. In order to compare the process of learning to perform sutured and mechanical microvascular anastomoses, two surgeons, with limited microvascular experience, performed 30 aortic and 30 femoral vein anastomoses in 30 Wistar rats. The methods compared were conventional suture, vascular closure system (VCS) and microvascular anastomatic coupler system (MAC). There were no inter-surgeon differences regarding patency or time to perform anastomoses. The average time to perform a suture anastomosis was 39 min (patency 80%). Anastomoses with the VCS system took 24 min (patency 25%), whereas the MAC couplers took 13 min to perform (patency 95%). There was a significant learning effect with sutures, but no obvious reduction in time to perform MAC coupler or VCS clip anastomoses was seen. MAC couplers were easiest to use, and allowed us to perform rapid anastomoses with high patency. 相似文献
18.
The value of functional and reconstructive surgery to the larynx in the treatment of malignant tumours to preserve residual function as far as possible has been clearly demonstrated. A further step in the development of functional surgery is classical microsurgery of the larynx which facilitates diagnosis and endoscopic microsurgical treatment of benign tumours. However, recently following the introduction of the carbon dioxide (CO2) laser it has been shown that it is possible to treat also malignant tumours of the larynx by endoscopic microsurgery.An incision made by the photo-thermal CO2 laser scalpel is characterized by the relative absence of bleeding, lack of post-operative oedema, rapid epithelialization without associated infection or cicatrization and this results in optimal post-operative laryngeal function.This paper presents the results of treatment of selected cases of T1 and T2 carcinoma of the glottis treated by CO2 laser excision under microlaryngoscopic control.In this series healing was rapid with minimal hospitalization and without a tracheostomy. Excellent functional results were achieved and the patient was able to return rapidly to his original occupation. It is our opinion that at present this treatment modality represents the optimal treatment for T1 and T2 carcinoma of the glottis. 相似文献
19.
Y Kiyoshige H Tsuchida M Hamasaki M Takayanagi Y Watanabe 《Journal of reconstructive microsurgery》1991,7(3):225-30; discussion 231-2
Experiments have been performed using CO2 laser-assisted microvascular anastomoses, and they demonstrated the following features, in comparison with conventional anastomoses: ease in technique; less time consumption; less tissue inflammation; early wound healing; equivalency of patency rate and inner pressure tolerance; but only about 50 percent of the tensile strength of manual-suture anastomosis. Six clinical applications in 16 vessels are reported, using this procedure. The preliminary results of these cases would appear to be the first successful replantations and free tissue transfers using CO2 laser-assisted microvascular anastomoses in man. The procedure offers increased safety and speed in microvascular anastomoses. 相似文献
20.
Methods of testing the strength of microvascular anastomoses are reviewed historically, in the light of recent applications to laser-assisted microvascular anastomosis techniques. The results of two experiments using hydrostatic distension to bursting point to determine the strengths of laser-assisted and conventionally sutured anastomoses of rat arteries and veins are presented. Considerable variation of the bursting pressures was found at any given time after anastomosis, and by the third postoperative day, the sutured vessels showed a significant fall in strength to their weakest level, with not as great a fall in the laser group. The sutured vessels were stronger than were the laser-anastomosed vessels, except at 3 days and after 6 weeks, when there were no significant differences between the two types of anastomosis. Aneurysmal vessels did not always burst at lower pressures than did nonaneurysmal vessels of comparable age. 相似文献