田基黄及其注射液的研究进展

本文综述了中药田基黄及田基黄注射液的研究进展,包括成分、药理、制剂工艺等的研究情况。     

田基黄中一种不稳定黄酮的研究

目的:对田基黄指纹图谱中保留时间约为50 min的不稳定成分进行研究.方法:用多种方法对田基黄乙醇提取物进行化学成分的分离,HPLC对成分进行跟踪,并用波谱技术鉴定其化学结构.结果:从田基黄中分得该化合物,经鉴定为Taxfolin-7-O-rhamnose(Ⅰ),该成分在空气中易被氧化成Vincetoxicoside(Ⅱ).结论:田基黄中存在不稳定的二氢黄酮化合物,该成分对判定田基黄药材质量以及改进注射液制备工艺都具有重要的意义.有关田基黄中黄酮类成分转化关系的研究目前尚未见报道.     

田基黄主要成分抗缺氧活性的研究

目的研究田基黄主要成分的抗缺氧活性。方法采用PC12细胞缺氧保护作用实验和DPPH法进行抗缺氧活性研究。结果田基黄中部分黄酮类化合物和酚酸类化合物具有较强的抗缺氧活性。结论田基黄中具有抗缺氧活性的化学成分均为分子量较小,酚羟基较多的化合物。     

田基黄的研究概况

田基黄为藤黄科植物地耳草Hypericum japomicumThunb的干燥全草。具有清利温热、散瘀消肿的功效。现已从田基黄中分离出黄酮类、酚类物质等有效成分。具有抑菌、抗肝损伤、抑制癌细胞等多种药理学功效。临床用于治疗急、慢性肝炎,传染性肝炎,原发性肝癌,伤寒、副伤寒等。现就田基黄的研究进展做一综述。1 化学成分 田基黄总黄酮中含有槲皮素、槲皮甙、异槲皮甙及3,5,7,3’,4’—五羟基黄酮-7-0-鼠李糖甙。酸性成分为间苯三酚衍生物,定名为JuponicineA、B、C、D。在田基黄的乙醚提取物中分离出     

田基黄注射液的定性鉴别

<正> 我县制药厂生产的田基黄注射液在临床上治疗病毒性肝炎,有较好的疗效,也颇受欢迎。为了控制田基黄注射液质量,但按照七七年版《中国药典》所收载的方法,在实际应用中常显阴性,而引起在质量上的许多争议。根据田基黄中主要成分为黄酮类化合物的特点,将田基     

复方田基黄胶囊的薄层鉴别研究

目的:建立复方田基黄胶囊的薄层鉴别方法,为其质量标准的制定提供依据。方法:采用薄层色谱法鉴别复方田基黄胶囊中的田基黄、虎杖、黄芪。结果:复方田基黄胶囊中的田基黄、虎杖、黄芪和对照药材薄层色谱主斑点位置一致;且定性鉴别斑点圆整,分离度好,易于区别。结论:所建立的方法简便、灵敏、准确、专属性强,可作为复方田基黄胶囊的质量控制方法之一。     

田基黄茎、花叶挥发油化学成分的研究

田基黄 (ＨｙｐｅｒｉｃｕｍｊａｐｏｎｉｃｕｍＴｈｕｎｂ .Ｈｙ)又名地耳草 ,为藤黄科金丝桃属植物 ,药用全草。是传统的清热利湿 ;散淤止痛、消肿解毒药 ,并有抑癌作用。田基黄对正常组织细胞无毒副作用 ,并有增强免疫功能的作用。我们对田基黄的化学成分进行研究以寻找新的活性化合物或先导化合物 ,为进一步开发利用田基黄的药用价值提供依据。1　样品及挥发油的提取将田基黄 (购于贵阳市药材市场 ,经贵州大学植物教研室廖海民副教授鉴定为腾黄科金丝桃属植物田基黄ＨｙｐｅｒｉｃｕｍｊａｐｏｎｉｃｕｍｍＴｈｕｎｂ .Ｈｙ)于 65℃恒温…     

田基黄注射液HPLC指纹图谱研究

本研究构建了田基黄注射液HPLC指纹图谱,利用指纹特征可全面监控田基黄注射液的质量.     

田基黄对大鼠急性肝损伤的保护作用

目的:研究田基黄对大鼠急性肝损伤的保护作用。方法:采用CC l4及D-氨基半乳糖(D-Gal)致大鼠急性肝损伤模型,观察田基黄提取液对肝损伤大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性的影响。结果:田基黄对CC l4及D-Gal所致的大鼠血清ALT、AST活性升高有明显的抑制作用。结论:田基黄对大鼠急性肝损伤具有保护作用。     

田基黄对人肝癌细胞HepG2增殖的影响

目的:探讨田基黄对人肝癌细胞增殖的影响。方法:体外培养人肝癌细胞株HepG2,用MTT法和流式细胞术(FCM)检测田基黄提取物及其含药血清作用于HepG2 24h后的细胞增殖情况。结果:MTT法结果表明,田基黄对HepG2增殖有抑制作用;FCM检测表明,田基黄阻止增殖中的HepG2进入S期。结论:田基黄能有效地抑制HepG2细胞增殖,提示阻滞细胞周期、抑制细胞增殖可能是田基黄治疗肝癌的一个机制。     

Evaluation of in-vivo wound healing activity of Hypericum patulum (Family: Hypericaceae) leaf extract on different wound model in rats

The methanol extract of Hypericum patulum Thumb. leaves were investigated for the evaluation of their wound healing potential on different experimental models of wounds in rats. The methanol extract of leaves (HPM), in the form of an ointment with two different concentrations (5% and 10% w/w ointment of leaf extract in simple ointment base) was evaluated for wound healing potential in an excision wound model and an incision wound model in rats. Both concentrations of the methanol extract ointment showed significant responses in both the wound types tested when compared with the control group. The effect produced by the extract ointment, in terms of wound contracting ability, wound closure time, regeneration of tissues at wound site, tensile strength of the wound and histopathological characteristics were comparable to those of a standard drug nitrofurazone ointment.     

Pharmacological activity of three commercial Hypericum perforatum preparations in mice

St John's Wort (Hypericum perforatum) is the main herbal species used to treat depression. The products available on the pharmaceutical and dietary supplement markets are obtained by a variety of preparation processes and their pharmacological effects may differ significantly. The purpose of this study therefore was to investigate the effect of different St John's Wort commercial preparations available on the French market. Only one preparation gave significant results in the forced swimming test.     

大蓟水提取物对正常大鼠离体胸主动脉环的舒张作用及其机制

目的：探讨大蓟水提取物对胸主动脉环的舒张作用及其作用机制。方法：采用累积加药法,检澳4大蓟水提取物（0．1,0．3和1．0mg／ml）对苯肾上腺素（PE）1．0umol／L预收缩的胸主动脉环张力的影响。结果：大蓟水提取物对离体大鼠内皮完整的胸主动脉环均有浓度依赖性的舒张作用,并对苯肾上腺素（PE）预收缩血管的舒张作用是内皮依赖性的。用一氧化氮合酶抑制剂L-NAME年口鸟苷酸环化酶抑制剂MB预处理后,两者的血管舒张作用均被阻断。但用环氧舍酶抑制剂吲哚关辛,不能阻断大蓟引起的舒张血管作用。结论：大蓟水提取物可能是通过NO-鸟苷酸环化酶途径产生内皮依赖性的血管舒张作用。     

山东贯叶连翘挥发油成分分析

目的研究山东产贯叶连翘挥发性化学成分。方法采用超临界CO2萃取法提取贯叶连翘全草挥发油,并通过气相色谱-质谱-计算机联用技术测定提取物的化学成分。结果从贯叶连翘挥发油中鉴定了47种化合物,其氧化石竹烯、斯巴醇、环十二烷、月桂酸等化合物为贯叶连翘挥发油的主要成分。结论山东产贯叶连翘挥发油成分与文献报道的其它地区产贯叶连翘挥发油成分有很大不同,说明地域和气候环境对植物的成分组成有很大的影响。     

Composition and antimicrobial activity of the essential oil of Hypericum rumeliacum subsp. apollinis (Boiss. & Heldr.)

The composition and the antimicrobial activity of the aerial parts of Hypericum rumeliacum are reported. Analysis was carried out by GC/MS. The major constituents were alpha-pinene (43.80%), beta-pinene (9.82%), dehydro-aromadendrene (6.81%) and alpha-copaene (5.41%). The essential oil showed a moderate in vitro activity against the six Gram negative and positive bacteria and a stronger one against the three-tested pathogenic activity.     

金丝桃属植物的HPLC指纹谱研究

目的 建立金丝桃属植物的HPLC指纹谱，对该属植物中所含的化学成分进行综合评价并为该属植物鉴定提供依据。方法 采用HPLC方法将获得的色谱数据转化为色谱指纹谱，据此对样品进行分折鉴别。结果 不同种金丝桃均显示了它们各自的指纹特征。结论HPLC指纹谱可以用来鉴别金丝桃属的不同种植物，该属植物种间的化学成分有较高的相似性，但各相应的成分含量差异显著。     

Antidepressant properties of some Hypericum canariense L. and Hypericum glandulosum Ait. extracts in the forced swimming test in mice

It has been shown in a previous work that the methanol extract obtained from the aerial part in blossom of Hypericum canariense L. and Hypericum glandulosum Ait. was active in the tetrabenazine and forced swimming test. In the present study, the central nervous effect of the aqueous, butanol and chloroform fractions obtained from the methanol extracts of these Hypericum species was investigated in mice, particularly in animal models of depression. It was found that the immobility time in the forced swimming test was significantly reduced by the butanol and chloroform fraction of both species assayed, producing no effects or only a slight depression on spontaneous motor activity when assessed in a photocell activity meter. In this regard, the efficacy of the chloroform extract from Hypericum glandulosum Ait. (500 mg/kg p.o.) in the forced swimming test was comparable to that of the tricyclic antidepressant imipramine (50 mg/kg p.o.). In addition, the Hypericum glandulosum chloroform fraction was also effective in antagonizing the ptosis induced by tetrabenazine. Moreover, Hypericum canariense butanol fraction and Hypericum glandulosum chloroform fraction produced a slight but significant hypothermia. Taken together, these data demonstrate that the butanol and chloroform fractions from Hypericum canariense and Hypericum glandulosum possess antidepressant-like effects in mice, providing further support for the traditional use of these plants in the Canary Islands folk medicine against central nervous disorders.     

贯叶金丝桃治疗膝关节骨性关节炎的临床研究

目的:探讨贯叶金丝桃治疗膝关节骨性关节炎的临床疗效。方法:我院2009年5月~2010年5月收治膝关节骨性关节炎患者180例,随机分为2组,每组90例。治疗组给予口服贯叶金丝桃制剂,对照组给予口服盐酸氨基葡萄糖片。结果:治疗组和对照组的临床症状均有明显改善,治疗组总有效率达92.2%,对照组总有效率为91.1%,2组疗效比较差异无统计学意义(P0.05)。结论:贯叶金丝桃片是治疗膝关节骨性关节炎的一种有效方法。     

In vitro cytotoxicity and antitumour properties of Hypericum mysorense and Hypericum patulum

The methanol extracts of the aerial parts of Hypericum mysorense and Hypericum patulum were tested for in vitro cytotoxicity on HEp-2, RD and Vero cell lines and antitumour activity using DLA and HEp-2 cell lines. The cell viability and morphological changes were assessed. Of these extracts, Hypericum patulum (stem) extract showed strong cytotoxicity against all the cell lines used. The CTC50 of the Hypericum patulum (stem) extract was 1.71 microg/mL for HEp-2, 1.53 microg/mL for RD and 2.23 microg/mL for Vero cell lines. The Hypericum patulum (leaves) and Hypericum mysorense (aerial parts) extracts showed moderate cytotoxicity and Hypericum patulum (aerial parts) extract did not show any cytotoxicity up to 1,000 microg/mL concentration. In the clonogenic assay, no colony formation was observed at a concentration of 300 micro g/mL and above for Hypericum mysorense (aerial parts), 400 microg/mL and above for Hypericum patulum (leaves) and 500 microg/mL and above for Hypericum patulum (stem) extracts. In the short term antitumour studies using DLA cells, 50% viability was observed in the concentration range 100-200 microg/mL for Hypericum patulum (leaves and stem) and 200-400 microg/mL for Hypericum mysorense (aerial) extract. In the long term antitumour activity using the HEp-2 cell line, no colony formation was observed over a concentration of 1.6 microg/mL for the Hypericum patulum (stem) extract.     

舒肝解郁胶囊治验典型实录

舒肝解郁胶囊是一种纯中药制剂,由贯叶金丝桃、刺五加组成,具有舒肝解郁,健脾安神的功效。笔者采用舒肝解郁胶囊治疗肝气不足诱发的抑郁症、冠心病、胆囊炎、胃炎,取得了较满意的疗效,并将其治疗经验进行总结报道,为临床用药提供一定的参考。