NEURONS DISCHARGE AND C-FOS EXPRESSION IN NTS FOLLOWING ELECTROACUPUNCTURE AT ACUPOINTS OF STOMACH MERIDIAN OF FOOTYANGMING

The nucleus of solitary tract(NTS) is a primary center for both visceral afferents and somatic afferents.Previous experiments demonstrated that NTS is associated with the connection of the stomach and acupoints in Stomach Meridian of Foot-Yangming(ST Meridian).In this study, extracellular recording and immunochemistry method were used to analyze the discharging neurons and c-Fos protein expression in NTS after electroacupuncture at different acupoints and non-acupoint.A total of 104 discharging neurons were detected in the NTS of 52 rats, of which, 86 were provided with complete data.After acupuncturing at Sibai(ST2), Zusanli(ST36), Neiting(ST44), Quanliao(SI18) and non-acupoint, the response neuron occurring rates(RNORs) in NTS were 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively.For RNORs, there was a significant difference among Sibai(ST2), Zusanli(ST36), Neiting(ST44), Quanliao(SI18) and nonacupoint(P0.01 or P0.05).Accordingly, in other 48 rats, the number of c-fos immunoreactive(c-fos-IR) neurons in the NTS after electroacupuncture(EA) at Sibai(ST2) was significantly higher than that of at other acupoints and non-acupoint(P0.05 or P0.01).Both EA at Zusanli(ST36) and Neiting(ST44) increased c-fos-IR neurons significantly compared with that of at Quanliao(SI18) and non-acupoint(P0.05 or P0.01), while there was no difference between EA at Quanliao(SI18) and nonacupoint(P0.05).The experiments demonstrated that the afferent convergence in NTS is different by body surface point stimulus, which suggested that NTS might be a primary center in the central nervous system(CNS) which received acupoint stimulus from ST Meridian.     

NEURON DISCHARGE AND C-FOS EXPRESSION IN NUCLEUS OF THE SOLITARY TRACT FOLLOWING ELECTROACUPUNCTURE AT ACUPOINTS OF STOMACH MERIDIAN OF FOOT-YANGMING

Objective: The nucleus of the solitary tract(NTS) is a primary center for both visceral afferents and somatic afferents.Previous experiments demonstrated that NTS is associated with connection of the stomach and acupoints in the Stomach Meridian of Foot-Yangming(ST Meridian).In this study, an extracellular recording and immunochemistry method was used to analyze the neuron discharge and c-fos protein expression in the NTS following acupuncture at different acupoints and non-acupoint.Methods: A total of 104 discharging neurons were detected in the NTS of 52 rats, of which, 86 were provided with complete data.Results: After acupuncture at Sibai(ST2), Zusanli(ST36), Neiting(ST44), Quanliao(SI18) and non-acupoint, the response neuron occurring rates(RNORs) in the NTS were 65.12%, 51.16%, 46.51%, 34.88% and 31.40%, respectively.For RNOR,there was a significant difference among ST2, ST36, ST44, SI18 and non-acupoint(P0.01 or P0.05).Accordingly, in other 48 rats, the number of c-fos immunoreactive(c-fos-IR) neurons in the NTS after electroacupuncture(EA) at ST2 was significantly higher than at other acupoints and non-acupoint(P0.05 or P0.01).Both EA at ST36 and at ST44 increased c-fos-IR neurons significantly as compared with EA at SI18 and non-acupoint(P0.05 or P0.01), while there was no difference between EA at SI18 and at non-acupoint groups(P0.05).Conclusion: The experiment demonstrated that the afferent convergence in the NTS was different by body surface point stimulus, which suggested that NTS might be a primary center in the central nervous system receiving acupoint stimulus from ST Meridian.     

针刺大鼠“四白”传入信息对孤束核神经元放电的影响

目的:比较足阳明经中远近腧穴及手太阳小肠经腧穴和非经非穴点的传入信息在孤束核(NTS)的差异。方法:采用细胞外记录方法,以足阳明胃经“四白”穴为刺激点,并以同名经的“地仓”和“内庭”、手太阳小肠经的“颧”、非经非穴“四白”旁开点为对照,在刺激点上用手针施以捻转手法刺激30 s,在NTS寻找对来自体表刺激点有反应的神经元。结果:52只大鼠记录到资料完整的86个NTS神经元中,针刺“四白”后NTS有反应神经元出现的机率(含兴奋和抑制性神经元)为65.12%,而针刺“地仓”“内庭”“颧”“四白”旁开点有反应神经元出现的机率分别为50.00%、46.51%、34.88%、31.40%。统计表明,针刺“四白”后NTS有反应神经元出现的机率均极显著地高于针刺“地仓”“内庭”“颧”“四白”旁开点后有反应神经元出现的机率(P<0.01);针刺“地仓”和“内庭”后NTS有反应神经元出现的机率均显著高于刺激“颧”和“四白”旁开点的有反应神经元出现机率(P<0.05)。针刺上述穴和非穴区,NTS神经元主要是以兴奋为主。针刺“四白”“地仓”“内庭”“颧”“四白”旁开点,其兴奋性神经元频率变化率分别为(35.08±4.80)%、(28.25±5.46)%(、27.57±4.87)%、(20.02±4.23)%(、18.55±2.49)%,“四白”与各组之间有显著性或极显著性差异(P<0.05或P<0.01),“地仓”“内庭”与“颧”“四白”旁开点亦有显著性或极显著性差异(P<0.05或P<0.01)。结论:与手太阳小肠经穴以及非经非穴点比较,足阳明经穴与NTS的关系更密切;足阳明经中远近腧穴的信息传入在NTS亦存在着一定的差异,这提示腧穴均有一定的特异性。     

大鼠头面部腧穴和胃扩张传入信息在孤束核的汇聚-c-fos表达的研究

目的 探讨电针大鼠头面部腧穴和胃扩张刺激诱导大鼠孤束核(Nucleus of Solitary Tract,NTS)的原癌基因c-fos表达及意义。方法 将SD大鼠随机分为6组:电针“四白”穴组、电针“四白”穴旁开组、电针“颊车”穴组、电针“颧髎”穴组、胃扩张模型组和空白对照组。采用免疫组织化学方法观察c-fos在NTS的表达。结果 电针“四白”穴组和胃扩张模型组NTS内均有较多的FOS样免疫反应(FLI)阳性神经元表达,且主要分布于内侧亚核(mNTS),并以延髓中尾段分布较多,其余亚核内较为稀疏,两组的FLI阳性神经元数目在mNTS比较,P>0.05;电针“颧髎”穴组和电针“颊车”穴组亦有一定数量的FLI阳性神经元表达;电针“四白”旁开组有少量FLI阳性神经元表达;空白组只有正常状态下的低FLI阳性神经元表达。结论 电针“四白”穴和胃扩张模型在mNTS内大量的FLI阳性神经元表达,两者的高表达提示来自电针“四白”穴和胃扩张刺激的感觉传入可能会在NTS发生汇聚、整合,从而影响胃的机能状态;此外,亦在形态学上为经脉脏腑相关跨神经节的初级中枢联系机制提供部分合理的解释。     

针刺足阳明经穴对大鼠胃肌电穴位特异性及胃电传出途径的实验研究

目的:探讨足阳明经对大鼠胃肌电穴位特异性影响及其传出途径研究.方法:选用足阳明经头面部四白、地仓穴,以及手太阳经颧髎穴及非经非穴四白旁开点,比较观察电针对禁食24h麻醉状态下大鼠胃肌电的影响;并通过腹注阿托品、六烃季胺、利血平神经阻断剂,观察传出途径阻断后电针四白穴对大鼠胃肌电的影响.结果:电针四白、地仓穴对胃电振幅、时程及峰簇数的发放具有一定的兴奋效应,其效应以四白穴作用最为明显,地仓次之,与针刺前比较均有统计学意义(P＜0.01),而电针颧髎、四白旁开点,对胃电活动则无明显作用,与针刺前比较均无统计学意义(P＞0.05);针刺四白穴能部分取消阿托品、六烃季胺对大鼠胃电的抑制效应,部分增强利血平后大鼠胃电的兴奋效应.结论:电针足阳明经对大鼠胃肌电具有一定的兴奋效应,其调整作用具有经脉、穴位的相对特异性.而电针对胃电的调整作用,与胆碱能神经、交感神经、肾上腺素能神经的参与有关.     

电针足阳明经穴对家兔胃粘膜损伤细胞保护作用的研究

本实验采用无水乙醇灌胃造成胃粘膜损伤后 ,分别电针足阳明经“四白”、“梁门”、“足三里”三个不同段代表穴 ,及“足三里”外 2cm的对照点 ,观察其对胃粘膜损伤后的细胞保护作用 ,以证实足阳明经与胃的相关性。结果发现 ,分别电针“四白”、“梁门”、“足三里”7日后 ,均能使胃粘膜损伤指数显著降低。电针“足三里”后 ,胃液及胃粘膜PGE2 与其他各组比较均显著增高 (P <0 0 5或 0 0 1 ) ,血清NO与模型组、电针“足三里”外 2cm组比较亦见明显增高 (P <0 0 5) ;电针“四白”、“梁门”、“足三里”以及空白组胃粘膜EGF与未经电针的模型组比较均有显著差异 (P <0 0 5或0 0 1 )。提示 ,电针家兔足阳明经不同节段的腧穴均对胃粘膜损伤细胞有保护作用 ,其中以“足三里”最满意 ,说明足阳明经与胃具有相关性 ,与此同时 ,同一经脉的穴位对相关脏腑的作用亦有着相对的特异性     

电针足阳明经穴对家兔胃运动和胃动素的影响

目的:观察电针足阳明经穴对胃运动和胃动素含量的影响及足阳明经与胃的相关规律.方法:40只家兔随机分为5组,即四白组、梁门组、足三里组、非经非穴组和模型组.运用消化道腔外电桥式应力传感器记录法,观察胃运动的变化,以放射免疫法检测胃动素的含量.结果:造模前,四白穴组和足三里穴组电针后,振幅指数显著升高,与针前比较差异有非常显著性意义(P＜0.01);造模后,四白穴组电针后,振幅指数显著升高,与针前比较差异显著(P＜0.01).电针各组穴位对收缩波数目百分率和胃动素的影响,针前针后比较,均无显著性差异(P＞0.05).结论:提示电针足阳明经四白、足三里穴,可使家兔胃运动振幅指数升高,足阳明经与胃具有相关性.     

基于集合可视化分析系统探索针灸治疗面肌痉挛的选穴规律

目的:基于集合可视化分析系统与Apriori算法探索针灸治疗面肌痉挛的选穴规律.方法:检索中国知网(CNKI)、维普网和万方数据建库至2019年9月27日中有关针灸治疗面肌痉挛的相关文献.对符合研究标准的文献进行相关内容提取及录入,利用R3.6.1进行集合可视化分析与Apriori算法分析针灸治疗面肌痉挛的选穴规律.结...     

Neurons in the NTS of rat response to gastric distention stimulation and acupuncture at body surface points

The nucleus of the solitary tract (NTS) is a primary center, not only for receiving visceral afferents, but also for somatic afferents. Previous experiments demonstrated that NTS might be a primary afferent center for connecting the Stomach Meridian (ST Medirian) and the Stomach. In this study, an extracellular recording method was used to analyze the firing neurons. The variety of NTS' neurons' responses to gastric distention (GD) and different body surface points were compared. In 52 rats, 104 firing neurons of NTS were recorded, in which there were 47 GD related neurons. For the 47 GD related neurons, there were 33 neurons responsive to acupuncture at sibai (ST 2, belonging to ST Meridian). Therefore, in the 47 GD related neurons, the response neuron occurring rate (RNOR) for acupuncture at ST 2 was 70.21%. Meanwhile in the 47 GD related neurons, when acupuncture was performed at Quanliao (SI 18, belonging to Small Intestine Meridian) and non-acupoint respectively, there were 18 and 17 firing neurons, and the RNOR were 38.30% and 36.17% respectively. The experiment demonstrated that there were some varieties in the response of NTS neurons to GD stimuli and acupuncture at different body surface points, which suggested that the ST Meridian was more intimately linked to the stomach and that the different acupoints do have some specificity.     

Clinical observation on electroacupuncture at local points plus point injection for Bell palsy during pregnancy

Objective

To observe the therapeutic effect of electroacupuncture (EA) at local points plus point injection on House-Brackmann facial nerve function classification grade (H-B) and facial disability index (FDI) in pregnant women with Bell palsy (BP).

Methods

A total of 40 eligible BP patients during pregnancy were randomized into a treatment group (n=20) and a control group (n=20). The treatment group was intervened by EA at Sizhukong (TE 23), Tongziliao (GB 1), Sibai (ST 2), Quanliao (SI 18), Yifeng (TE 17), Dicang (ST 4), Jiache (ST 6) and point injection at Zusanli (ST 36) and Yanglingquan (GB 34) on the affected side, 30 min every time, 10 sessions as a course, for 2 courses in total while control group by EA with the same points, manipulation and courses as the treatment group.

Results

After the treatment, the H-B classification in the treatment group was better than that in the control group, with a significant inter-group difference (P<0.05); after the treatment, the change of FDI score in the treatment group was significantly different from that in the control group (P<0.05).

Conclusion

EA at local points plus point injection can produce a more significant efficacy than EA in the treatment of BP during pregnancy.

     

Repeated Application of Electroacupuncture Ameliorates Hyperglycemia in Obese Zucker Diabetic Fatty Rats

Electroacupuncture (EA) was investigated for lowering the blood glucose (BG) in fasting male obese Zucker fatty diabetic (ZDF) rats aged 10–17 weeks. Anesthesia provided satisfactory chemical restraint to enable repeated EA. Animals in Groups 1, 2 and 3 were anesthetized on Days 1, 3, 5, 8, 10 and 12. Group 1 (n = 4) received no EA (controls), Group 2 (n = 4) EA at Zhongwan and Guanyuan acupoints, and Group 3 (n = 4) EA at both Zusanli acupoints. BG was measured at 10 and 20 minutes, and EA was applied for 30 minutes, after which BG was measured again. Group 2 had a significantly lower baseline BG at 20 minutes on Days 5, 8 and 12 and significantly less change in BG over 30 minutes on Days 3 and 5 than Group 1 (p < 0.05). Group 3 showed a significant decrease in the mean baseline BG compared to Group 1 in Week 1 (p < 0.05). Thus, repeated EA using Zhongwan and Guanyuan acupoints was effective in lowering the baseline BG and modulating the change in the BG in anesthetized animals.     

The Beneficial Effects of Electroacupuncture at PC6 Acupoints (Neiguan) on Myocardial Ischemia in ASIC3 −/− mice

This study aims to investigate the possible mechanisms of electroacupuncture (EA) at PC6 to improve myocardial ischemia (MI) by regulating the cardiac transient outward potassium current channel (Ito). According to the random number table, the mice were divided into six groups of six mice each: control group, MI group, PC6, LU7 (Lieque-point), ST36 (Zusanli-point), and nonacupoint group. Mice in the control group were injected with saline (20 mg/kg, 24 hours interval), and the other ASIC3 ?/? mice were injected subcutaneously twice with isoproterenol (ISO) (20 mg/kg, 24 hours interval). In the preexperiment, 5 mg/kg, 10 mg/kg, 20 mg/kg, and 30 mg/kg of ISO were used, and the results showed that 5 mg/kg and 10 mg/kg of ISO both could induce acute MI, but shorter duration of sustained MI. On the other hand, an injection of 30 mg/kg can make the mice experience arrhythmia or die immediately, and EA was operated at PC6, LU7, ST36 acupoints, and nonacupoint in the mice of PC6, LU7, ST36, and nonacupoint groups, respectively, after injecting twice. Then Western blotting techniques (Western Blot) were used to analyze the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KchIP2. The results of this experiment showed that the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KChIP2 in MI group were significantly lower than those in the control group (p < 0.01). Compared with MI group, the results of PC6, LU7, and ST36 groups obviously increased (p < 0.05). Furthermore, the expressions of PC6 group were higher than LU7 group and ST36 group (p < 0.05). And electrocardiogram's T-waves showed obvious pathological changes in the MI group compared to the control group (p < 0.01). After EA, the abnormal T-waves voltage of ECG in PC6, LU7, and ST36 groups was improved (p < 0.05). In addition, the rate change of PC6 group was larger than that of both LU7 and ST36 groups (p < 0.05). But the T-waves voltage of the nonacupoint group was not significantly different than that of the MI group (p > 0.05).     

基于复杂网络社团结构划分的古代治疗面瘫腧穴配伍规律研究

目的:分析古代针灸治疗面瘫的腧穴配伍规律及其处方的优化。方法:在全面收集古代针灸治疗面瘫的文献基础上,构建古代面瘫针灸处方数据库,采用复杂网络二分网络社团结构划分的方法,分析古代针灸治疗面瘫的腧穴配伍规律特点,并对处方优化进行研究。结果:古代针灸治疗面瘫的核心穴位多以水沟、地仓、颊车、合谷、风池、足三里、内庭、听会、承浆、上关为主。核心穴对多以地仓、颊车、水沟、风池、内庭、合谷、足三里、列缺、上关、二间之间相互配伍。优化处方核心组成以听会、风池、风市、悬钟、地仓、颊车、足三里、大椎、百会、曲池、肩髃、发际为主,选穴分布多以胃经、大肠经、督脉为核心。头面部腧穴的使用频率明显高于其他部位;选穴配伍多以特定穴为主,其中以交会穴使用最频繁。结论:局部选穴是古代针灸治疗面瘫的首选,特定穴是古代针灸治疗面瘫处方中的核心用穴。     

正常成年人下肢穴位疼痛阈值检测

目的:比较人体下肢穴位与非穴位痛阈的差异,探讨穴位的相对特异性。方法:制定人体随机测量数据表和人体体表图。选无心理和生理疾病的66名成年人,用Von Frey测量小腿和足部感受器阈值,分析对比穴位与非穴位痛阈值的异同。结果:膀胱经承山、昆仑、京骨和束骨的痛阈显著低于非穴位,昆仑的痛阈值最低(P<0.05);足三里同一水平节段穴位足三里、阴陵泉和阳陵泉与同一水平节段的非穴位比较,穴位处的痛阈值明显低于非穴位(P<0.05);下巨虚同一水平节段的穴位下巨虚、外丘、阳交、飞扬、漏谷与同一水平节段的非穴位比较,穴位处的痛阈值明显低于非穴位(P<0.05);解溪同一水平节段的中封、商丘、解溪、申脉相比较,解溪穴的痛阈值最高(P<0.05);分布于趾间的荥穴之间比较,侠溪穴和大都穴的痛阈值较高(P<0.05);位于足少阴肾经的水泉、太溪、筑宾和足太阴脾经的漏谷之间比较,漏谷的痛阈值最高,水泉次之(P<0.05)。结论:穴位的痛阈值明显低于非穴位,且穴位之间的痛阈值也存在差异,表明穴位确实具有相对特异性,从而为临床选穴规范化提供了依据。     

经脉线与非经脉线微循环血流灌注量的比较及针刺对其的影响

目的:比较常态下胃经与其两侧旁开的非经对照点的微循环血流灌注量有无差异以及电针对其的影响。方法:应用激光多谱勒血流仪,对21名健康成年志愿者进行观察。先将418-1型测试探头固定在所要测试部位,然后将一次性静脉留置针在胃经的上巨虚、梁丘、阴市、伏兔4个穴位和阴市与伏兔中点的非穴位点及其左右旁开1cm对照点插至1cm深度,导入光纤,尽量减少直射光的干扰,分别检测常态下及电针足三里时经脉线与非经脉线微循环血流灌注量情况。结果:①上巨虚、梁丘、阴市、伏兔4个穴位和阴市-伏兔中点的非穴位点的深部组织微循环血流灌注量都高于其两侧旁开非经对照点(P<0.01);②与电针前比,电针后胃经线下深部组织微循环血流灌注量较针前略有增高,但差异无统计学意义(P>0.05);③电针刺激可以使胃经循行线下的微循环更加均匀、顺畅。结论:沿胃经循行线下深部组织中的微循环血流灌注量均高于两侧非经对照部位,电针刺激可以使胃经循行线下的微循环更加均匀、顺畅,证实深部组织中的血流是形成循经红外辐射轨迹的主要热源,也可能是经脉参与人体机能调节活动的重要基础。     

Effects of Acupuncture at the Acupoints of 12 Meridians on Gastrointestinal and Cardiac Electricity in Healthy Adults

The effect of acupuncture at the acupoints of 12 meridians on gastrointestinal and cardiac electricity in healthy adults was studied. Specific regulation between meridian points and viscera was also investigated. An electrogastrogram (EGG), electrointestinogram (EIG), carotid pulse graph, phonocardiogram and electrocardiogram were obtained in 30 healthy adults before and after acupuncture at various acupoints of 12 meridians. The effects of acupuncture on the amplitude and frequency of the EGG, EIG, pre-ejection period and the left ventricular ejection time were then analyzed. Acupuncture revealed that LR3 decreased the amplitude of the EGG while LI11 (Quchi), SJ5 (Waiguan), ST36 (Zusanli), SP9 (Yinlingquan) and SI6 (Yanglao) increased the amplitude. Multiple comparisons among the latter five acupoints indicated that there were significant differences between SP9, LI11, SJ5 and ST36 (p < 0.01, p < 0.01, p < 0.05) and SI6, LI11 and SJ5 (p < 0.01, p < 0.05). SP9 effected EGG amplitude the most, followed by SI6, ST36, SJ5 and LI11. Four acupoints increased the amplitude of the EIG (p < 0.05), including HT5 (Tongli), GB34 (Yanglingquan), SP9 and SI6. No significant differences were observed between these acupoints, but SI6 showed the most obvious effect on EIG amplitude, followed by GB34, SP9 and HT5. No significant effects on the frequency of the gastrointestinal slow wave or on cardiac function indexes were observed. Effects were observed, however, on pre-ejection period and left ventricular ejection time. Routine acupuncture had no detrimental effects on the stomach, intestine and heart in healthy adults, but instead regulated physiological function within a normal range. These findings demonstrate the existence of specific connections between the meridian points and the viscera. The results suggest that multiple meridians control the same viscus, and the same meridian can regulate the functions of multiple viscera.     

Anticonvulsant Effect of Berberis integerrima L. Root Extracts in Mice

Berberis integerrima is a member of Berberidaceae family. Berberine is one of the main constituents of this plant, having neuroprotective effect on central nervous system diseases. In this study, the anticonvulsant activity of methanolic extract, and hydromethanolic fraction, and chloroform fraction of B integerrima was assessed. The anticonvulsant effect of B integerrima was investigated using both pentylenetetrazole (PTZ) and maximal electroshock (MES)-induced seizure models. The LD50 value of the methanolic extract was 302.676 mg/kg. In the PTZ test, methanolic extract (140 and 200 mg/kg, i.p., p < 0.01), hydromethanolic fraction (200 mg/kg, p < 0.01), and chloroform fraction (200 mg/kg, p < 0.01) increased the onset time of hind limb tonic extensions (HLTEs). The protective effect against mortality (convulsion survivors/animals tested) was 2/8 in methanolic extract, and 3/8 in hydromethanolic fraction at a dose of 200 mg/kg and in chloroform fraction at a dose of 140 mg/kg. In the MES test, this plant did not display any significant effect in reducing HLTE duration. According to phytochemical screening, methanolic extract contained alkaloids and tannins. The present study, conducted in mice, indicated that B integerrima has anticonvulsant activity in PTZ-induced seizures. It is concluded that B integerrima may be useful in petit mal epilepsy.     

针刺足阳明经穴对家兔胃窦平滑肌细胞舒缩长度的影响

目的 :从细胞水平研究足阳明经与胃相关的特异性。方法 :将 45只家兔随机分为生理盐水组、阿托品模型组、针刺足阳明经穴组、针刺足少阳经穴组、针刺足太阳经穴组 ,每组 9只。分组处理后 ,取胃窦平滑肌 ,制成活的单个平滑肌细胞悬液 ,在显微镜下以测微尺测定细胞舒缩长度。结果 :针刺足阳明经穴组的胃窦平滑肌细胞长度明显短于其他各组 (P <0 .0 1 )。结论 :针刺足阳明经穴能使经阿托品阻断的胃窦平滑肌细胞产生明显的收缩效应 ,进一步证实了足阳明经与胃之间存在特异性的联系。     

Clinical observation on 48 cases of peripheral facial paralysis treated by acupoint-penetrating acupuncture

针刺患侧阳白透鱼腰、地仓透颊车、下关透颊车、四白透颧髎、百会透太阳、印堂透山根、攒竹透晴明、合谷透劳宫，并根据症状增加穴位，治疗了48例周围性面瘫患者，痊愈39例，显效7例，无效2例，治愈率为81．2％，有效率95．8％。     

电针大鼠“四白”穴和胃扩张传入信息在孤束核的汇聚——c-fos表达的研究

目的:探讨电针足阳明经“四白”穴和胃扩张刺激诱导大鼠孤束核(NTS)的原癌基因c -fos表达及意义。方法:将SD大鼠随机分为4组:电针“四白”穴组、电针“四白”穴旁开0 .5cm组、胃扩张模型组和空白对照组。采用免疫组织化学方法观察c- fos在孤束核的表达。结果:电针“四白”穴组和胃扩张模型组孤束核内Fos样免疫反应(FLI)阳性神经元均主要分布于内侧亚核,以延髓的中尾段分布较多;电针“四白”穴组在内侧亚核内FLI神经元数目与胃扩张模型组比较无明显差异(P >0 .0 5 ) ,与“四白”穴旁开组比较,差异有非常显著性意义(P <0 .0 1 )。结论:电针“四白”穴和胃扩张刺激的感觉传入可能在孤束核发生汇聚、整合,是针刺“四白”穴调节胃功能的中枢途径之一。