黑乳海参中两个新的四环三萜化合物

目的：研究黑乳海参体内的化学成分.方法：应用多种色谱技术对黑乳海参体内的化学成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构.结果：分离得到了2个三萜皂苷元类化合物,应用化学方法和各种光谱方法鉴定其结构为：3β,17α-二羟基-7,9-二烯-海参烷;3β,17α-二羟基-9,22-二烯-海参烷.结论：2个化合物均为新化合物.命名为黑乳参皂苷元A和B.是我国学者首次从海参中分离得到的三萜皂苷元类化合物.     

沙海参中的三个海参皂苷

目的：研究沙海参体内的化学成分，寻找结构新颖的三萜皂苷类化合物。方法：应用多种色谱技术对沙海参体内的化学成分进行分离纯化，根据化合物的理化性质和波谱数据鉴定其结构。结果：分离得到三个三萜皂苷化合物，分别为arenicolaside　A（1），fuscocineroside C（2）和holothurin A（3）。结论：化合物1为一个新的三萜皂苷化合物，化合物2、3均为首次从沙海参中分离得到.     

叶瓜参中的三个海参皂苷

目的：研究叶瓜参体内的化学成分，寻找结构新颖的三萜皂苷类化合物。方法：应用多种色谱技术对叶瓜参体内的化学成分进行分离纯化，根据化合物的理化性质和波谱数据鉴定其结构。结果：分离得到三个三萜皂苷化合物，分别为frondosideA6（1），frondoside A（2）和frondoside A1（3）。结论：化合物1为一个新的三萜皂苷。     

玉足海参中具有细胞毒活性的三萜皂苷成分研究

 目的 研究玉足海参（Holothuria leucospilota）具有生物活性的三萜皂苷类成分。方法 应用大孔树脂柱色谱、正相硅胶柱色谱、反相硅胶柱色谱和HPLC对玉足海参体内三萜皂苷活性成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果 分离并鉴定了3个三萜皂苷类化合物,分别为：leucospilotaside D (1)、holothurin B (2) 和holothurin B₂ (3)。 化合物1~3对人宫颈癌细胞HeLa,人胃癌细胞BEL-7402和人乳腺癌细胞MCF-7的抑制率较强。结论 化合物1为首次从玉足海参中分离得到。3个化合物均显示显著的体外细胞毒活性。     

玉足海参中的三萜皂苷成分

目的：研究玉足海参中具有生物活性的三萜皂苷。方法：应用正相硅胶柱色谱、反相硅胶柱色谱和高效液相色谱的方法分离和纯化三贴皂苷,并通过光谱和化学方法鉴定化合物结构。结果：从玉足海参中分离得到7个三萜皂苷,分别鉴定为leucospilotasideA（1）,leucospilotasideB（2）,leucospilotasideC（3）,holothurinB（4）,holothurinB2（5）,echinoside13（6）,and holothurinA（7）。结论：化合物2为新的海参三萜皂苷,化合物1,3,5和6为首次从该种海参中分得。     

黑乳海参化学成分的研究

目的：对黑乳海参体内的化学成分进行研究。方法：利用大孔树脂、硅胶、ODSRP-C18硅胶、Sephadex LH-20和HPLC等色谱方法进行分离纯化,根据所得化合物的理化性质和波谱数据（1H-NMR,13C-NMR,2D-NMR和ESI-MS）,鉴定其结构。结果：分离得到4个化合物,分别鉴定为：海参皂苷A（holothuria A,I）,胸腺嘧啶（thymine,II）,尿嘧啶（uracil,III）,胆甾醇（cholesterol,IV）。结论：4个化合物均为首次从该种动物中得到。     

黑乳海参皂苷nobilisideⅠ和nobilisideⅡ的体外抗真菌及抗肿瘤活性

目的:阐明黑乳海参皂苷nobilisideⅠ和nobilisideⅡ的体外抗真菌及抗肿瘤活性。方法:对黑乳海参的总提取物采取硅胶柱色谱及半制备HPLC等分离手段进行活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB法评价化合物的抗肿瘤活性。结果:分离得到了2个新的三萜皂苷元类化合物,命名为nobilisideⅠ和nobilisideⅡ。2个新化合物均显示了较强的体外抗真菌及抗肿瘤活性。结论:通过该研究为研制新的抗肿瘤药物提供了先导化合物,为充分开发利用我国的海洋生物资源提供了物质基础和科学依据。     

花刺参中两个新的三萜皂苷

目的：研究花刺参体内的化学成分。方法：应用Sephadex LH-20，正相硅胶柱层析、反相硅胶柱层析和HPLC对花刺参体内化学成分进行分离纯化，根据化合物的理化性质和波谱数据鉴定其结构。结果：分离并鉴定了2个三萜皂苷类化合物：3-O-[3-O-甲基-β-D-吡喃葡萄糖-（1→3）-β-D-吡喃木糖-（1→3）-β-D-吡喃葡萄糖-（1→2）-β-D-吡喃木糖]-海参烷-7-烯-3β，23S-二醇（Ⅰ），3-O--[3-O-甲基-β-D-吡喃葡萄糖-（1→3）-β-D-吡喃木糖-（1→3）-β-D-吡喃葡萄糖-（1→2）-β-D-吡喃木糖]-海参烷-8-烯-3β，23S-二醇（Ⅱ）。结论：2个化合物均为新化合物，命名为variegatusideA和variegatusideB。     

利用稻瘟霉分生孢子追踪分离黑乳海参活性成分

目的：研究黑乳海参中海参皂苷类活性成分。方法：以稻瘟霉分生孢子活性追踪结果为指导,运用多种层析分离技术和光谱学方法对黑乳海参中的海参皂苷性成分进行分离并鉴定其结构。结果：分离并鉴定了12个化合物,包括7个新的海参皂苷（nobiliside D-J）。所有皂苷类化合物均对稻瘟霉有活性,且对P388小鼠白血病和A549人肺癌细胞株具有显著或较强的细胞毒作用。结论：通过该研究为研制新的抗肿瘤药物提供了先导化合物,为充分开发利用我国的海洋生物资源提供了物质基础和科学依据。     

皂苷治疗糖尿病及并发症的研究进展

皂苷广泛存在于自然界中,单子叶植物和双子叶植物中均有分布。近年从海洋生物如海参、海星以及动物体内也分离得到活性皂苷。皂苷按化学结构分为三萜皂苷和甾体皂苷。三萜皂苷的皂苷元是三萜类化合物,基本骨架可分为五环三萜和四环三萜两大类。甾体皂苷的皂苷元是甾体衍生物,基     

Triterpene Glycosides from Sea Cucumber Holothuria scabra with Cytotoxic Activity

Objective To study the new triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity.Methods Triterpene glycosides from H.scabra were separated and purified by chromatography on...     

Bioactive triterpene glycosides from the sea cucumber Holothuria fuscocinerea

Bioassay-guided fractionation of the active n-BuOH extract of the sea cucumber Holothuria fuscocinerea resulted in the isolation of three new triterpene glycosides, fuscocinerosides A (1), B (2), and C (3), along with two known glycosides, pervicoside C (4) and holothurin A (5), as active compounds causing morphological abnormality of Pyricularia oryzae mycelia. Compounds 1-5 possess the same tetrasaccharide moiety, 3-O-methyl-beta-D-glucopyranosyl-(1-->3)-beta-D- glucopyranosyl-(1-->4)-beta-D-quinovopyranosyl-(1-->2)-4-O-sodiumsulfato-beta-D-xylopyranosyl, linked to C-3 of holostane triterpene aglycones that differ in their side chains and 17-substituents. Their structures were elucidated by extensive spectral studies as well as chemical evidence. All the glycosides showed in vitro cytotoxicity against two human tumor cell lines.     

Cytotoxic and antifungal triterpene glycosides from the Patagonian sea cucumber Hemoiedema spectabilis

Two new sulfated triterpene glycosides, hemoiedemosides A (1) and B (2), have been isolated from the Patagonian sea cucumber Hemoiedema spectabilis. Their structures have been established by a combination of spectroscopic analysis (NMR and FABMS) and chemical transformations. Both glycosides present the same aglycon and differ in the degree of sulfation of the tetrasaccharide chain. Hemoiedemoside B (2) is a new example of a small number of trisulfated triterpene glycosides from sea cucumbers belonging to the family Cucumariidae. Glycosides 1 and 2 exhibit considerable antifungal activity against the phytopathogenic fungus Cladosporium cucumerinum, while the semisynthetic desulfated derivative 1a is less active.     

Two new cytotoxic and virucidal trisulfated triterpene glycosides from the Antarctic sea cucumber Staurocucumis liouvillei.

Two new trisulfated triterpene glycosides, liouvillosides A (1) and B (2), have been isolated from the Antarctic sea cucumber Staurocucumis liouvillei. Their structures have been elucidated by spectroscopic analysis (NMR and FABMS) and chemical transformations. Liouvillosides A (1) and B (2) are two new examples of a small number of trisulfated triterpene glycosides from sea cucumbers belonging to the family Cucumariidae. Both glycosides were found to be virucidal against herpes simplex virus type 1 (HSV-1) at concentrations below 10 microg/mL.     

Holothurins B(2), B(3), and B(4), new triterpene glycosides from mediterranean sea cucumbers of the genus holothuria

Triterpene glycosides of three species of the Mediterranean sea cucumbers Holothuria polii, Holothuria tubulosa, and Holothuria sp. were studied. Three new monosulfated biosides, holothurins B(2) (1), B(3) (2), and B(4) (3), along with the previously known holothurins A (4) and B (5) were isolated from the sea cucumber H. polii. Triterpene glycosides belonging to holothurin A and B groups were found in H. tubulosa, while only one individual glycoside, holothurin A (4), was isolated from Holothuria sp. Structures of new substances were elucidated on the basis of spectral data (2D NMR and MS). The significance of holothurins as chemotaxonomic markers of the animals belonging to the genus Holothuria and taxonomic status of some representatives of the holothurians studied are discussed.     

土茯苓二氢黄酮醇类成分研究

目的 :研究土茯苓的化学成分。方法 :用硅胶、大孔树脂、ODS柱色谱及HPLC方法进行分离纯化 ,根据理化性质和光谱数据进行结构鉴定。结果 :分离鉴定了 5个二氢黄酮醇苷类化合物 ,分别为落新妇苷 (1) ,新落新妇苷 (2 ) ,异落新妇苷 (3) ,新异落新妇苷 (4 ) ,(2R ,3R) 花旗松素-3′-O-β-D-吡喃葡萄糖苷 (5 )。 结论 :化合物 2 ,4 ,5为首次从该植物中分离得到。     

A New Triterpene Saponin from Gynostemma pentaphyllum

Objective To study the triterpene saponins from Gynostemma pentaphyllum with antitumor activities. Methods The 75% EtOH extract of G. pentaphyllum was used for isolation by silica gel column chromatography and preparative HPLC. The structures of pure compounds isolated were identified by the spectral analysis and chemical evidence. Results Two compounds were isolated and identified as 23(S)-3β,20ξ,21ξ-trihydroxy-19-oxo-21,23-epoxydammar-24-ene 3-O-α-L-rhamnopyranosyl (1→2)-[β-D-xylopyranosyl (1→3)]-β-D-arab...