高效液相色谱法测定人红细胞膜脂肪酸含量及组成

本文采用高效液相色谱法测定了４２例正常成人红细胞膜脂肪酸（ＦＡ）含量及组成。结果表明：该法分离效果好、定量准确。重复性变异系数平均５．５％，回收率平均９４．７％。人红细胞膜主要由廿二碳六烯酸（Ｃ２２６）、花生四烯酸（Ｃ２０４）、亚油酸（Ｃ１８２）、软脂酸（Ｃ１６０）、油酸（Ｃ１８１）和硬脂酸（Ｃ１８０）等六种ＦＡ组成，其中花生四烯酸含量最高，其次为硬脂酸。不饱和脂肪酸占７１．６％。     

脑梗塞患者红细胞变形能力与膜磷脂组分的关系

为探讨脑梗塞患者红细胞膜磷脂各组分对红细胞变形能力的影响，采用高效液相色谱法对58例脑梗塞患者和26名健康人的红细胞膜磷脂各组分进行了测定，并同时检测了其红细胞胆固醇含量及红细胞变形能力。结果表明，脑梗塞患者红细胞膜磷脂酸胆碱（PC）、磷脂酸乙醇胺（PE）含量降低，胆固醇（CHO）含量升高，红细胞变形能力降低。直线相关分析，膜PC与红细胞滤过指数呈显著负相关。结论：脑梗塞患者红细胞膜你在以PE、PC变化为主的磷脂代谢紊乱，膜PC异常可能是影响红细胞变形能力的一个重要因素。     

白血病患者红细胞膜RIA

白血病患者血清中铁蛋白台量测定应用于临床已有十多年的历史，报道屡见不鲜，而有关白血病患者红细胞膜铁蛋白含量测定的报道较少。为此，我们进行了探讨，并分析了红细胞膜铁蛋白与血清铁蛋白和血红蛋白的关系，现报道如下。     

红细胞膜与红细胞流变性

红细胞膜与红细胞流变性北京医科大学医学物理教研室陈亮，王鸿儒红细胞膜与红细胞流变性质的相关性研究，在国内已开始关注，但报道不多，作者仅就国外在这方面的研究进展作一简要介绍。红细胞膜的化学组成与分子模型红细胞膜包裹在红细胞胞浆的表面，它的主要成份是脂类...     

一种测量红细胞膜弹性的新方法

德国的Ｆｌｉｅｇｅｒ和法国的Ｇｒｅｂｅ新近提出了一种测量红细胞膜弹性的方法，将细胞置于离心机中加确定大小的载荷，再用激光衍射仪观测其变形程度，用正常红细胞和戊二醛或二酰胺处理的红细胞做试验，证明此法简便易行，用血量少，每感性高，具有良好的应用前景。     

糖皮质激素对人红细胞膜微粘度的效应

糖皮质激素对人红细胞膜微粘度的效应金淑敏，许澍淮，徐仁宝（中国医学科学院基础医学研究所北京１００００５）严重创伤、休克时，血浆糖皮质激素（ＧＣ）可升高８～１０倍，但机体没有肾上腺皮质功能亢进的表现，相反，治疗上还要给大量ＧＣ。很多研究结果表明：应激和...     

人红细胞膜带3蛋白结构研究进展

人红细胞膜带3蛋白由911个氨基酸组成,可分为N端胞质域和C端跨膜域。最新研究结果表明,α螺旋含量较高的跨膜域往返跨膜12次。在细胞膜中,带3蛋白主要以二聚体和四聚体(二聚体的二聚体)形式存在;尽管其中的单体可独立地转运阴离子,然而二聚体中的两个单体之间存在着相互作用。     

一种测量红细胞膜弹性的新方法

德国的Ｆｌｉｅｇｅｒ和法国的Ｇｒｅｂｅ新近提出了一种测量红细胞膜弹性的方法：将细胞置于离心机中加确定大小的载荷，再用激光衍射仪观测其变形程度。用正常红细胞和戊二醛或二酰胺处理的红细胞做试验，证明此法简便易行，用血量少，每感性高，具有良好的应用前景。     

反相高效液相色谱法测定血清中的5-羟色胺

建立了一种简便、快速测定血清中5-羟色胺的反相高效液相分析方法。采用的色谱柱为Bondapak C18不锈钢柱(5μm,250mm×4.6mmi.d.),流动相为:甲醇—水—乙酸(70:30:0.1 V/V),流速:1ml/min,荧光监测器,激发波长278nm,发射波长333 nm。结果表明:该方法线性关系良好(r=0.99992),5-羟色胺测定的线性范围为0.10~2.00μg/mL,最低检测限为0.02μg/mL(S/N=3)。5-羟色胺在血清样品中的加标回收率为92.37%~100.12%。该测定方法结果准确,可用于临床研究。     

高效液相色谱法测定的雌激素受体临床应用于人乳腺癌

应用体积排阻高效液相色谱法(SEHPLC)分析13例人乳腺癌组织的雌激素受体。此法能迅速检出雌激素受体的8S及4S亚单位,为选择对内分泌治疗敏感的乳腺癌病人提供了新的依据。     

流动相对西罗莫司异构体在反相液相色谱上分离效果影响的研究

研究不同流动相对西罗莫司异构体在反相液相色谱上分离效果的影响,为准确测定西罗莫司的含量提供依据。色谱柱采用YMC J’sphere ODS-H80,流动相分别采用不同体积比例的水/乙腈(40/60,35/65,30/70,v/v)或水/甲醇(20/80,15/85,10/90,v/v)。结果表明:以水/乙腈或水/甲醇二元溶剂为流动相时,随着有机溶剂比例的增加,西罗莫司异构体(β峰与γ峰)的保留时间都变短,峰宽都变狭窄,分离度也相应降低。选择流动相水/乙腈(35/65)或水/甲醇(15/85),西罗莫司的异构体(β峰与γ峰)均能完全分离。与流动相水/乙腈(35/65)相比,以水/甲醇(15/85)作流动相,西罗莫司β异构体色谱峰的保留时间更短,峰宽更狭窄,是水/甲醇与水/乙腈流动相体系中的最佳条件。     

Separation of High‐Impact Polypropylene Using Interactive Liquid Chromatography

Fractionation techniques such as temperature rising elution fractionation or crystallization analysis fractionation fail to fractionate the ethylene–propylene (EP) copolymers, which are a component of high‐impact polypropylene (hiPP), according to their chemical composition. High‐temperature high‐performance liquid chromatography separates blends of EP‐copolymers and polypropylene. The elution volume of the EP‐copolymers depends linearly on the average content of ethylene. The separation according to the chemical composition was hyphenated with size exclusion chromatography. In this way, the relationship between the distribution with regard to chemical composition and molar mass, that is, the full chemical heterogeneity of hiPP, was revealed for the first time.     

Characterization of Porcine Zona Pellucida Antigens by Immunoaffinity Chromatography and by High-Pressure Liquid Chromatography

ABSTRACT: In the present work, 500 and 50,000 porcine zonae pellucidae were solubilized using Lithium-3,5-diiodosalicylate. The zona antigens were purified by immunoaffinity chromatography (IAC) on immobilized antizona immunoglobulin G (IgG). The antizona-IgG was raised by immunization of female rabbits with 500 heat-solubilized porcine zonae. Four antigens could be detected following IAC: ZP I/1 (M_r = 42,000), ZP II/1 (M_r = 67,000), ZP II/2 (M_r = 32,000), ZP III/1 (M_r = 17,000). In a parallel experiment, 50,000 zonae were solubilized in a similar manner and the mixture was analyzed by high-pressure liquid chromatography (HPLC) using a protein column. Altogether, 9 protein peaks that contained the antigens ZP I/1, ZP II/1, ZP II/2, and ZP III/1 could be detected following HPLC. The carbohydrate composition is characteristic for O-glycosidic-glycoproteins. ZP II/1 and ZP II/2 are probably in close association within the zona. Based on the reaction of the antigens with antibodies induced by intact and heat-solubilized zonae, it is postulated that only ZP I/1 and ZP II/l are expressed on the surface in intact zonae.     

Separation of Parent Homopolymers from Nonpolar Block Copolymers by Means of Liquid Chromatography under Limiting Conditions of Enthalpic Interactions

Parent homopolymers are baseline‐separated from the block copolymers with nonpolar arms in a single step with the help of liquid chromatography under limiting conditions of enthalpic interactions. The small differences in the chain solubility are employed to apparently originate two similar but independent liquid chromatography retention mechanisms, enthalpic partition (absorption) and phase separation (precipitation). For the first time, the option is demonstrated to separate a complex polymer system with a tandem of two independent liquid chromatography procedures.     

Structure Determination of Polyacetals by Liquid Chromatography and Hyphenated Techniques

Summary: The structure of polyacetal copolymers with respect to their end groups is investigated. Several techniques, such as liquid chromatography, MALDI‐TOF MS, IR and NMR spectroscopy, as well as combined techniques like 2D chromatography have been applied. Cyclic oligomers and polymers with formyl, hydroxy, and aliphatic end groups are identified. 2D chromatography shows that all the separated species are homogenously distributed with respect to their molar mass.

2D chromatograms of sample F97B3.     

Thermodynamic Differences Between Deuterated and Protonated Polystyrenes Evaluated Using Reversed Phase Liquid Chromatography

Summary: Deuterated compounds are generally considered to possess very similar properties to their protonated analogues. Reversed phase liquid chromatography (RPLC) was used to measure thermodynamic interactions between deuterated and protonated n‐butyl polystyrenes and a C18 stationary phase. Significant differences in enthalpies and entropies of interaction were observed for deuterated and protonated n‐butyl polystyrenes. This is very important because many techniques, such as small‐angle neutron scattering and neutron reflectivity, assume that the interaction of these molecules are identical.

Structure of n‐butyl polystyrenes.