石荠芋总黄酮抗炎作用及其机制研究

目的观察石荠荸总黄酮抗炎作用并对其机制进行初步研究。方法采用二甲苯致小鼠耳肿胀，冰醋酸致小鼠腹腔毛细血管通透性增加，角叉菜胶致小鼠足跖肿胀及大鼠气囊炎等炎症模型，观察石荠芋总黄酮对不同炎症模型的抗炎作用，并测定蛋白质、前列腺素E2（PGE2），一氧化氮（NO），丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。结果石荠芋总黄酮能显著降低小鼠毛细血管通透性，抑制小鼠二甲苯致耳肿胀，减轻小鼠足跖肿胀，降低炎症组织PGE2含量，抑制角叉菜胶致大鼠气囊炎模型中渗出液总蛋白质含量，降低血浆NO，MDA含量，增强血浆SOD活性。结论石荠荸总黄酮具有明显的抗炎作用，其抗炎作用可能与其降低血管通透性、抑制PGE2，NO等炎症介质生成及增强清除氧自由基、抗脂质过氧化能力有关。     

痔疮熏洗液的抗炎作用及机制研究

目的:研究痔疮熏洗液的抗炎作用并对其作用机制进行初步探讨。方法:采用二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足跖肿胀及角叉菜胶致小鼠气囊炎3种模型考察痔疮熏洗液的抗炎作用,并测量炎足组织中前列腺素E2(PGE2)、丙二醛(MDA)、蛋白质含量和超氧化物歧化酶(SOD)活性,以及气囊炎渗出液中白细胞数量和一氧化氮(NO)含量,研究痔疮熏洗液的抗炎机制。结果:痔疮熏洗液高、中、低剂量组可显著抑制二甲苯所致小鼠耳廓肿胀;明显降低角叉菜胶致炎大鼠足跖肿胀度,降低角叉菜胶致大鼠炎足组织中PGE2、MDA、蛋白含量,增加SOD活性;降低角叉菜胶致小鼠气囊炎渗出液中NO含量和白细胞数。结论:痔疮熏洗液具有明显的抗炎作用,其抗炎机制可能与抑制PGE2、NO等炎症介质产生,抑制白细胞游出,抑制脂质过氧化、增强清除自由基有关。     

五味藤抗炎作用及其机制初探

目的研究五味藤水提物的抗炎作用并探寻其部分机制。方法通过巴豆油诱导小鼠耳肿胀、角叉菜胶诱导小鼠足爪肿胀、醋酸致小鼠腹腔毛细血管通透性增加、角叉菜胶致大鼠胸膜炎和棉球诱导大鼠肉芽肿模型,观察五味藤的抗炎作用;检测角叉菜胶致大鼠胸膜炎大鼠胸腔渗出液中总蛋白、NO及PGE2含量的测定和对小鼠炎性组织中PGE2含量的测定,初步探索五味藤的抗炎作用机制。结果五味藤水提物对巴豆油诱导的小鼠耳肿胀、角叉菜胶诱导的小鼠足爪肿胀、棉球诱导的大鼠肉芽肿、醋酸致小鼠腹腔毛细血管通透性增加和角叉菜胶致大鼠胸膜炎具有抑制作用,对角叉菜胶致大鼠胸膜炎大鼠胸腔液中总蛋白、NO及PGE2有抑制作用。结论五味藤有较强的抗炎作用,抗炎机制与抑制PGE2、NO生成及抑制总蛋白渗出有关。     

枫蓼提取物的抗炎作用及机制研究

目的:研究枫蓼提取物的抗炎作用及其作用机制。方法:采用二甲苯致小鼠耳肿胀、冰醋酸致小鼠腹腔毛细血管通透性增高及角叉菜胶致大鼠足肿胀炎症模型,灌胃给药5d,研究枫蓼提取物的抗炎作用,通过测定大鼠炎足组织中白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、前列腺素E2(PGE2)、一氧化氮(NO)及丙二醛(MDA)的含量,分析枫蓼提取物的抗炎机制。结果:枫蓼提取物23.4、46.8g/kg能明显抑制小鼠耳肿胀,降低小鼠腹腔毛细血管通透性、减少渗出。角叉菜胶致大鼠足跖肿胀实验结果表明,枫蓼提取物16.2、32.4g/kg能显著抑制角叉菜胶致大鼠足跖肿胀,提取物16.2、32.4g/kg能不同程度的降低炎足组织中TNF-α、IL-1β、PGE_2和MDA的含量,但对NO无明显影响。结论:枫蓼提取物具有明显的抗炎作用,其抗炎机制可能与抑制炎性介质释放及抗氧化有关。     

西瓜藤石油醚提取物抗炎作用及其机制探讨

目的：研究西瓜藤石油醚提取物的抗炎作用并探讨其作用机制。方法：采用二甲苯致小鼠耳肿胀模型、小鼠腹腔毛细血管通透性增高模型、鸡蛋清致小鼠足肿胀模型、棉球致大鼠肉芽肿模型、角叉菜胶致大鼠足肿胀模型,观察西瓜藤石油醚提取物的抗炎作用。并测定棉球肉芽肿模型大鼠血清中一氧化氮（NO）、一氧化氮合酶（NOS）的含量和肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）水平;测定角叉菜胶足肿胀模型大鼠肿足炎性渗出物中丙二醛（MDA）、前列腺素E2（PGE2）、5-羟色胺（5-HT）、组织胺（His）、蛋白含量及血清中 NO、NOS的含量及总超氧化物歧化酶（SOD）活性,探讨可能的抗炎机制。结果：西瓜藤石油醚提取物对二甲苯致小鼠耳肿胀、小鼠腹腔毛细血管通透性、蛋清致小鼠足肿胀、棉球致大鼠肉芽肿、对角叉菜胶致大鼠足肿胀,均有不同程度的抑制作用。能降低棉球肉芽肿大鼠血清中NO、NOS含量和TNF-α、IL-1β水平,能降低角叉菜胶足肿大鼠肿足炎性渗出物中MDA、PGE2、5-HT、His及蛋白含量,抑制血清中NO和NOS的升高,提高SOD的活性。结论：西瓜藤石油醚提取物具有一定的抗炎作用,其机制可能与影响炎症介质的产生和抗氧化作用有关。     

消痔栓抗炎作用及其作用机理的实验研究

目的：观察消痔栓的抗炎作用并初步探讨其作用机制。方法：采用小鼠耳肿胀模型观察消痔栓的抗炎作用,同时测定新鲜鸡蛋清致小鼠炎性足中PGE2、MDA、NO、Pr含量和SOD活力。结果：消痔栓能显著抑制二甲苯所致小鼠耳肿胀,明显降低新鲜鸡蛋清致炎足炎性组织中的PGE2、MDA、NO、Pr含量,显著增加足跖局部炎症组织中SOD活性。结论：消痔栓具有明显的抗炎作用,其机制可能与抑制PGE2等炎症介质合成、抑制脂质过氧化、增强自由基清除能力有关。     

鼻炎口服液抗炎作用的实验研究

目的：观察鼻炎口服液的抗炎作用及其机制。方法：采用组胺诱发大鼠毛细血管通透性增高，二甲苯致小鼠耳廓肿胀，大鼠棉球肉芽肿，角叉菜胶致大鼠足跖肿胀与大鼠气囊滑膜炎模型，评价鼻炎口服液的抗炎作用并探讨其作用机制。结果：鼻炎口服液对组胺诱发大鼠毛细血管通透性增高、二甲苯致小鼠耳廓肿胀、大鼠棉球致肉芽组织增生均有显著的抑制作用，能显著降低大鼠角叉菜胶性足跖肿胀及炎症组织内PGE2含量，明显减少大鼠气囊滑膜炎模型渗出物中白细胞和CRP计数，并能明显提高炎性渗出物中SOD-1的活性。结论：鼻炎口服液具有较好的抗炎作用，该效应与降低PGE、CRP水平，提高SOD-1活性有关。     

三棱丸抗炎镇痛作用研究

目的:研究三棱丸的镇痛和抗炎作用。方法:采用小鼠热板法和扭体法观察镇痛作用;采用二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性、角叉菜胶诱导大鼠足肿胀、棉球诱导大鼠肉芽组织增生的试验模型观察抗炎作用;并测定角叉菜胶诱导大鼠足肿胀炎性渗出液中前列腺素E2(PGE2)的含量。结果:三棱丸能显著延长小鼠的疼痛反应时间,明显减少小鼠的扭体次数;抑制醋酸致小鼠腹腔毛细血管通透性增高,减轻小鼠耳肿胀和大鼠足爪肿胀反应;抑制棉球诱导大鼠肉芽组织增生;能明显减少大鼠角叉菜胶性炎症渗出液中PGE2含量。结论:三棱丸具有显著的镇痛和抗炎作用,其机制可能与抑制PGE2产生与释放有关。     

角花胡颓子醇提物抗炎镇痛作用研究

目的研究角花胡颓子醇提物的抗炎和镇痛作用。方法采用热板法和醋酸致小鼠扭体实验观察镇痛作用;采用二甲苯致小鼠耳肿胀及皮肤毛细血管通透性、角叉菜胶致大鼠足肿胀和大鼠棉球肉芽肿实验模型观察抗炎作用;并测定角叉菜胶致炎足渗出液中PGE2和组胺含量。结果角花胡颓子醇提物能显著延长热致痛小鼠痛阈时间,明显减少醋酸致痛小鼠扭体次数;对二甲苯致耳肿张及皮肤毛细血管通透性、角叉菜胶致足肿胀和棉球诱导肉芽肿增生均有抑制作用;能明显减少角叉菜胶性炎症渗出液中PGE2和组胺的含量。结论角花胡颓子醇提物有明显的抗炎镇痛作用。     

鸭跖草抗炎活性部位筛选及抗炎机制

目的筛选鸭跖草抗炎作用的活性部位,并研究其抗炎机制。方法采用二甲苯致小鼠耳肿胀模型、冰醋酸致小鼠腹腔毛细血管通透性模型、角叉菜胶诱导大鼠足跖肿胀,进行抗炎活性筛选;测定胸膜炎大鼠胸腔渗出液丙二醛(MDA)、前列腺素E2(PGE2)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)水平以及肺组织一氧化氮(NO)、PGE2、TNF-α、IL-1β和MDA水平,探讨抗炎机制。结果与5%吐温-80组比较,水溶部位高、中剂量(30、15 g/kg)对二甲苯致小鼠耳廓肿胀、急性炎症导致的腹腔毛细血管通透性、角叉菜胶引起的大鼠足跖肿胀均有显著抑制作用(P0.05,0.01),其余部位活性弱或基本无活性;鸭跖草水溶部位组(15 g/kg)能抑制胸腔液MDA、PGE2、TNF-α、IL-1β水平升高(P0.01);鸭跖草水溶部位组(15 g/kg)能抑制肺组织NO、MDA、PGE2、TNF-α、IL-1β水平升高(P0.01)。结论鸭跖草水溶部位为抗炎活性部位,抗炎作用可能与其抑制炎症介质产生和抗氧化作用有关。     

白藜芦醇抗炎作用机制的初步研究

 目的观察白藜芦醇的抗炎作用并对其抗炎机制进行初步探讨。方法采用切除双侧肾上腺大鼠足肿胀、冰醋酸致大鼠胸膜炎及角叉菜胶致大鼠气囊炎3种炎症模型,观察白藜芦醇对不同炎症模型的抗炎作用,并测量白细胞数量、蛋白质含量、前列腺素E₂(PGE₂)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、超氧阴离子(O₂.-)含量变化。结果白藜芦醇高、中剂量组可减轻去双侧肾上腺大鼠足肿胀程度;减少了冰醋酸致大鼠胸膜炎渗出液量和蛋白质含量,使血清NO含量降低,SOD活性增强;抑制了角叉菜胶致大鼠气囊炎模型中白细胞游出及蛋白质渗出,降低PGE₂,NO,MDA,O₂.-生成量。结论白藜芦醇具有明显的抗炎作用,其抗炎作用不依赖于肾上腺的存在,可能与其降低血管通透性、抑制白细胞游出,抑制PGE₂、NO等炎症介质产生及增强清除氧自由基、抗脂质过氧化能力有关。     

Sclareol exhibits anti-inflammatory activity in both lipopolysaccharide-stimulated macrophages and the λ-carrageenan-induced paw edema model

Sclareol (1) is a natural fragrance compound used widely in the cosmetic and food industries. Lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the λ-carrageenan-induced edema mouse paw model were applied to examine the anti-inflammatory potential of 1 and its possible molecular mechanisms. The experimental results obtained demonstrated that this compound inhibited cell growth, nitric oxide (NO) production, and the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in LPS-stimulated macrophages. Compound 1 also reduced paw edema, the tissue content of NO, tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), iNOS and COX-2 protein expression, and neutrophil infiltration within the tissues after λ-carrageenan stimulation. The present study suggests that the anti-inflammatory mechanisms of 1 might be related to a decrease of inflammatory cytokines and an increase of antioxidant enzyme activity.     

鸡枞菌粉提取物镇痛抗炎作用的研究

目的:研究鸡枞菌粉提取物的镇痛抗炎作用。方法:采用醋酸扭体实验,甲醛致痛模型,二甲苯致耳肿胀模型及角叉菜胶致足肿胀模型观察鸡枞菌粉及其提取物镇痛抗炎作用。同时对小鼠炎症渗出液中PGE2,SOD,MDA,NO和NOS进行测定。结果:鸡枞菌粉提取物均能降低小鼠醋酸引起的扭体次数,减少甲醛致痛实验中小鼠舔足时间;抑制二甲苯致小鼠耳肿胀及角叉菜胶引起的小鼠足肿胀。对炎症介质也有较好的抑制作用。结论:鸡枞菌粉提取物具有明显镇痛抗炎作用。     

Effect of total phenolics from Laggera alata on acute and chronic inflammation models

The anti-inflammatory effect of total phenolics from Laggera alata (TPLA) was evaluated with various in vivo models of both acute and chronic inflammations. In the acute inflammation tests, TPLA inhibited significantly xylene-induced mouse ear oedema, carrageenan-induced rat paw oedema and acetic acid-induced mouse vascular permeability. In the carrageenan-induced rat pleurisy model, TPLA significantly suppressed inflammatory exudate and leukocyte migration, reduced the serum levels of lysozyme (LZM) and malondialdehyde (MDA), increased the serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and also decreased the contents of total protein, nitric oxide (NO) and prostaglandin E(2) (PGE(2)) in the pleural exudates. In the chronic inflammation experiment, TPLA inhibited significantly cotton pellet-induced rat granuloma. These results indicated that TPLA possesses potent anti-inflammatory activity on acute and chronic inflammation models. Its anti-inflammatory mechanisms are probably associated with the inhibition of prostaglandin formation, the influence on the antioxidant systems, and the suppression of LZM release. Furthermore, the total phenolic content of Laggera alata and its main component type was quantified, and its principle components were isolated and authenticated. Acute toxicity studies revealed that TPLA up to an oral dose of 8.5 g/kg body weight was almost nontoxic in mice.     

拔葜抗炎作用及对炎症介质的影响

目的:研究拔葜抗炎作用及作用机制。方法:观察拔葜对角叉菜胶所致大鼠足肿胀、炎症组织和血清中前列腺素E2(PGE2)、丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)含量的影响。结果:拔葜灌胃给药能明显抑制角叉菜胶所致正常大鼠和去肾上腺大鼠足肿胀,150g/kg拔葜可降低肿胀足爪组织中PGE2及血清中NO、MDA含量,90、150g/kg拔葜可降低肿胀足爪组织中NO含量。结论:拔葜可能通过影响炎症介质释放而起到抑制角叉菜胶性大鼠足肿胀作用。     

Anti-inflammatory effects of Scoparia dulcis L. and betulinic acid

The aims of this study intended to investigate the anti-inflammatory activity of the 70% ethanol extract from Scoparia dulcis (SDE) and betulinic acid on λ-carrageenan-induced paw edema in mice. The anti-inflammatory mechanism of SDE and betulinic acid was examined by detecting the levels of cyclooxygenase-2 (COX-2), nitric oxide (NO), tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and malondialdehyde (MDA) in the edema paw tissue and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) in the liver. The betulinic acid content in SDE was detected by high performance liquid chromatography (HPLC). In the anti-inflammatory model, the results showed that SDE (0.5 and 1.0 g/kg) and betulinic acid (20 and 40 mg/kg) reduced the paw edema at 3, 4 and 5 h after λ-carrageenan administration. Moreover, SDE and betulinic acid affected the levels of COX-2, NO, TNF-α and IL1-β in the λ-carrageenan-induced edema paws. The activities of SOD, GPx and GRd in the liver tissue were increased and the MDA levels in the edema paws were decreased. It is suggested that SDE and betulinic acid possessed anti-inflammatory activities and the anti-inflammatory mechanisms appear to be related to the reduction of the levels of COX-2, NO, TNF-α and IL1-β in inflamed tissues, as well as the inhibition of MDA level via increasing the activities of SOD, GPx and GRd. The analytical result showed that the content of betulinic acid in SDE was 6.25 mg/g extract.     

Analgesic and anti-inflammatory activities of aqueous extract from Glycine tomentella root in mice

In the present study, we have investigated the analgesic effect of the aqueous extract of the root of Glycine tomentella (AGT) using models of acetic acid-induced writhing response and formalin test, the anti-inflammatory effect of AGT using model of lambda-carrageenan-induced paw edema. In order to investigate the anti-inflammatory mechanism of AGT, we have detected the activities of glutathione peroxidase (GPx) and glutathione reductase (GRx) in the liver and the levels of malondialdehyde (MDA) and NO in the edema paw. In the analgesic test, AGT (0.5 and 1.0 g/kg) decreased the acetic acid-induced writhing response and the licking time on the late phase in the formalin test. In the anti-inflammatory test, AGT (0.5 and 1.0 g/kg) decreased the paw edema at the third, fourth, fifth and sixth hour after lambda-carrageenan administration, and increased the activities of SOD, GPx and GRx in the liver tissue and decreased the MDA level in the edema paw at the third hour after lambda-carrageenan injection. However, AGT could not affect the NO level which induced by lambda-carrageenan. These results suggested that AGT possessed analgesic and anti-inflammatory effects. The anti-inflammatory mechanism of AGT might be related to the decrease in the level of MDA in the edema paw via increasing the activities of SOD, GPx and GRx in the liver.     

Evaluation of antiinflammatory activity of the total flavonoids of Laggera pterodonta on acute and chronic inflammation models

The antiinflammatory effect of the total flavonoids of Laggera pterodonta (TFLP) was evaluated with various in vivo models of both acute and chronic inflammation. In the acute inflammation tests, TFLP significantly inhibited xylene-induced mouse ear oedema, carrageenan-induced rat paw oedema and acetic acid-induced mouse vascular permeability. In the carrageenan-induced rat pleurisy model, TFLP efficiently suppressed inflammatory exudate and leukocyte migration, reduced the serum levels of lysozyme (LZM) and malondialdehyde (MDA), increased the activity of serum superoxide dismutase (SOD), and also decreased the contents of total protein, nitric oxide (NO) and prostaglandin E2 (PGE2) in the pleural exudates. No marked effect of TFLP on the activity of serum glutathione peroxidase (GSH-PX) was observed. In the chronic inflammation experiment, TFLP inhibited cotton pellet-induced rat granuloma. The antiinflammatory mechanisms of TFLP are probably associated with the inhibition of prostaglandin formation, influence on the antioxidant systems and the suppression of LZM release. The acute toxicity study revealed that TFLP was nontoxic in mice up to an oral dose of 7.5 g/kg body weight.     

Analgesic and anti-inflammatory activities of the methanol extract of Elaeagnus oldhamii Maxim. in mice

We investigated possible mechanisms of analgesic and anti-inflammatory activities of the methanol extract from the leaf of Elaeagnus oldhamii Maxim. (EO(MeOH)). EO(MeOH) was evaluated for its analgesic activity in acetic acid-induced writhing response and formalin test, and anti-inflammatory effect was examined by λ-carrageenan-induced paw edema assay. We detected the activities of GPx, GRd and SOD in the liver, and the levels of inflammatory mediators including IL-1β, IL-6, TNF-α, COX-2, MDA and NO in the edema paw to investigate the mechanism of action against inflammation. Total polyphenol, flavonoid and flavanol contents of EO(MeOH) were detected to explore its antioxidant activities. Results showed that, in the analgesic test, EO(MeOH) decreased acetic acid-induced writhing response and the licking time in the late phase of formalin test. In the anti-inflammatory test, EO(MeOH) decreased paw edema at the 2nd, 3rd, 4th and 5th h after λ-carrageenan had been injected. EO(MeOH) increased the activities of SOD and GPx in liver tissue and decreased MDA, NO, IL-1β, IL-6, TNF-α and COX-2 levels in paw edema tissue at the 3rd h after λ-carrageenan-induced inflammatory reaction. EO(MeOH) exhibited abundant polyphenol, flavonoid and flavanol contents. In HPLC fingerprint test of EO(MeOH), two index ingredients, ursolic acid and pomolic acid, were isolated from EO(MeOH) and were exhibited in HPLC chromatographic analysis. The results demonstrated analgesic and anti-inflammatory effects of EO(MeOH). It was indicated that the anti-inflammatory mechanism of EO(MeOH) may be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx and GRd in the liver. Additionally, EO(MeOH) decreased IL-1β, IL-6, TNF-α and COX-2 levels in the edema paw. The results suggested its value in future development of herbal medicine for the treatment of inflammatory diseases.     

芫花根乙醇提取物的抗炎活性

目的 阐明芫花根乙醇提取物(EERD)的抗炎活性。方法 EERD对急性炎症的抑制作用采用组胺诱导的小鼠毛细血管通透性增加、角叉菜胶诱导的大鼠足肿胀以及小鼠网状内皮系统(RES)对异物的吞噬作用进行分析。对慢性炎症的抑制作用以福氏完全佐剂诱导的多发性关节炎和棉球诱导的大鼠肉芽肿进行分析。结果 EERD在剂量为40mg／kg时能明显地抑制小鼠毛细血管通透性增加和抑制大鼠足肿胀；在剂量为30mg／kg时对RES的吞噬能力有提升作用、对大鼠肉芽肿和多发性关节炎表现出显著抑制作用。EERD也能抑制丙二醛(MDA)、前列腺素E2(PGE2)、一氧化氮(NO)、肿瘤坏死因子-α(TNF—α)和白细胞介素-1β(IL-1β)的形成，增强超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活力并钝化诱导型氮氧化物合酶(iNOS)的活性。结论 EERD的抗炎活性主要是通过抑制脂质过氧化反应和炎症介质的释放、增强SOD和CAT的活力、钝化iNOS的活性以及提升RES的吞噬作用实现的。