BD Influx流式细胞分选仪参数调试和最佳条件的设定

目的：探索BD Influx流式细胞仪分选细胞亚群的调试参数和最佳设定条件。方法：使用两种质控试剂Rainbow Beads和Accudrop Beads作为实验材料,对BD Influx流式细胞仪的针孔、液流、荧光通道、前向角散射光信号、液滴频率、振幅和液滴延迟等参数进行优化。结果：使用型号为100μm的喷嘴,在液流聚焦清楚、液柱打入废液收集管的正中央、荧光光斑进入针孔中央且最亮、前向角散射（FSC）光光斑进入针孔中央且最亮、断点最高、液流分叉呈稳定的亮点、振幅为5.20＆#177;1.00以及液滴延迟为35.20＆#177;2.00等最佳设定条件下,其细胞样本分选纯度可达99%,工作效率可达95%。结论：流式细胞仪分选参数条件的设定,可为获得高纯度、高活性的细胞提供快速调试方法。     

BD公司两种流式细胞仪测定CD4+T细胞结果比较研究

目的 比较BD公司两种机型(BD FACSCalibur和BD FACSVia)流式细胞仪检测艾滋病病毒(HIV)感染者CD4+T细胞的绝对数及其仪器相关性能.方法 用BD公司的FACSCalibur和FACSVia两种流式细胞仪对该中心采集的48份艾滋病感染标本进行平行测试,并用SPSS软件对测定结果进行分析.结果 ...     

BD流式细胞仪操作系统的升级与其他

本文以BD流式细胞仪为例,介绍了如何对医疗设备中计算机配置进行升级以适应当前的工作需要及其对医疗工作的重要意义。     

高端流式细胞分选仪的选型评价

介绍了高端流式细胞仪的工作原理、结构组成和主要技术评价指标,归纳并阐述了各主要技术指标的异同和含义,列举了Beckman CoulterMoFlo XDP、BD FACSAria Ⅲ和BD InFlux3种高端流式细胞分选仪主要机型的选型比较,指出了高端流式细胞仪的选型应根据实际需求有所侧重,使采购选型的决策信息丰富、可信、科学。     

BD流式细胞仪数据处理系统的升级

我院1997年购入美国BD公司的FACS Calibur流式细胞仪,其数据处理系统已不能满足目前的工作需要,若重新购置一套数据处理系统,价格昂贵.因此,我们对其进行系统软硬件的升级,用很低的成本解决了上述问题.现将升级过程介绍如下.     

BD FACSAriaIII流式细胞仪在血液学中的使用分析研究

目的：对BD FACSAriaIII流式细胞仪在应用中出现的故障原因进行分析、研究,为在科研工作中正确使用该仪器提供依据。方法：通过对2012-2013年实验血液学国家重点实验室技术平台BD FACSAriaIII流式细胞仪的使用情况进行统计,对使用过程中出现的常见故障和需要由厂商工程师现场解决的故障进行统计,总结出高发故障的原因及解决方法。结果：通过对BD FACSAriaIII流式细胞仪的故障进行分析,为高通量流式细胞分选仪的正确使用提供指导,提高仪器运行的稳定性。结论：正确的使用和良好的维护有助于保证BD FACSAriaIII流式细胞仪的正常运行,从而确保科研工作的顺利进行。     

流式细胞分选仪（BD FACSAria Ⅲ）分选中常见问题分析及处理

流式细胞分选技术在基础科研和临床医学研究中的应用广泛。为提高分选操作效率,保障分选过程中故障能够及时处理,文章对BD(FACSAria Ⅲ)流式细胞分选仪分选中常见问题分析和处理进行简单介绍。     

流式细胞仪的应用体会

流式细胞仪是集激光、电子、光电测量、细胞荧光、单克隆等技术为一体的仪器,具有灵敏度高、重复性好、特异性强、方法灵活、分析速度快等优点,从流式细胞仪实际应用的角度介绍、总结了用流式细胞仪标本处理、染色、检测分析过程中的质量控制体会。     

流式细胞仪在睾丸生精过程研究中的应用

流式细胞仪在生殖研究中起着重要的作用,简要介绍了流式细胞仪的基本原理以及流式技术对生精细胞的检测,分析流式技术在生殖毒理、睾丸肿瘤、放射线损伤和生精细胞凋亡中的运用,阐述流式仪对各级生精细胞的分选。     

流式细胞术分选调试初探

流式细胞术是以流式细胞仪为工具来完成细胞等生物粒子理化特性研究的重要方法，它的主要特点是在单细胞水平上对大量细胞进行快速、准确。多参数的定量分析。流式细胞术分为流式细胞测量术和流式细胞分选术两大部分，而其中流式细胞分选术是现代科研型流式细胞仪不可缺少的功能之一，它可以每秒钟上千个细胞的速度进行分选，其纯度可达到９９％，回收率达到９８％。它的主要用途是使被指定的细胞或细胞器、质点等从一个群体上被分选出来，以便于科研工作者进一步鉴定细胞或克隆培养回收试验之用。但是细胞分选之前，必需在仪器上进行一系列…     

A critical evaluation of a flow cytometer used for detecting enterococci in recreational waters

The current U. S. Environmental Protection Agency-approved method for enterococci (Method 1600) in recreational water is a membrane filter (MF) method that takes 24 hours to obtain results. If the recreational water is not in compliance with the standard, the risk of exposure to enteric pathogens may occur before the water is identified as hazardous. Because flow cytometry combined with specific fluorescent antibodies has the potential to be used as a rapid detection method for microorganisms, this technology was evaluated as a rapid, same-day method to detect enterococci in bathing beach waters. The flow cytometer chosen for this study was a laser microbial detection system designed to detect labeled antibodies. A comparison of MF counts with flow cytometry counts of enterococci in phosphate buffer and sterile-filtered recreational water showed good agreement between the two methods. However, when flow cytometry was used, the counts were several orders of magnitude higher than the MF counts with no correlation to Enterococcus spike concentrations. The unspiked sample controls frequently had higher counts than the samples spiked with enterococci. Particles within the spiked water samples were probably counted as target cells by the flow cytometer because of autofluorescence or non-specific adsorption of antibody and carryover to subsequent samples. For these reasons, this technology may not be suitable for enterococci detection in recreational waters. Improvements in research and instrument design that will eliminate high background and carryover may make this a viable technology in the future.     

流式细胞仪在血液学检验中的应用

流式细胞仪（flow cytometer，FCM）是一项集激光技术、电子物理技术、光电测量技术、计算机技术以及细胞荧光化学技术、单克隆抗体技术为一体的新型高科技仪器。在医学研究领域中，流式细胞仪能够快速分析单个细胞的多种特性，它既可以定性也可以定量，适于大量标本的检测。主要介绍FCM在血液病临床与研究中的应用。     

流式细胞仪构成与工作原理

流式细胞仪是分选细胞结构与功能，准确迅速选标记细胞的仪器。本文讲述流式细胞仪的主要组件，工作原理及其实际应用。     

低剂量辐射小鼠骨髓细胞凋亡率的变化

目的:探讨低剂量射线暴露小鼠骨髓细胞凋亡率的变化及意义。方法:以Co60射线照射ICR小鼠,制备不同时间段的骨髓单个核细胞的单细胞悬液,用PI/FITC-Annexin V双染法,通过流式细胞仪检测样品的凋亡率。结果:骨髓单个核细胞早期凋亡率于照射后6 h明显增高;晚期凋亡率随辐射剂量增大而增高。白细胞计数及骨髓细胞微核率于24~30 h与对照组比较差异有显著性(P<0.05,P<0.01)。结论:小鼠骨髓细胞凋亡率的变化可及时反映射线对小鼠骨髓细胞的影响。细胞凋亡率检测可能成为低剂量射线接触人群的早期监控指标。     

A critical evaluation of a flow cytometer used for detecting enterococci in recreational waters

The current U. S. Environmental Protection Agency-approved method for enterococci (Method 1600) in recreational water is a membrane filter (MF) method that takes 24 hours to obtain results. If the recreational water is not in compliance with the standard, the risk of exposure to enteric pathogens may occur before the water is identified as hazardous. Because flow cytometry combined with specific fluorescent antibodies has the potential to be used as a rapid detection method for microorganisms, this technology was evaluated as a rapid, same-day method to detect enterococci in bathing beach waters. The flow cytometer chosen for this study was a laser microbial detection system designed to detect labeled antibodies. A comparison of MF counts with flow cytometry counts of enterococci in phosphate buffer and sterile-filtered recreational water showed good agreement between the two methods. However, when flow cytometry was used, the counts were several orders of magnitude higher than the MF counts with no correlation to Enterococcus spike concentrations. The unspiked sample controls frequently had higher counts than the samples spiked with enterococci. Particles within the spiked water samples were probably counted as target cells by the flow cytometer because of autofluorescence or non-specific adsorption of antibody and carryover to subsequent samples. For these reasons, this technology may not be suitable for enterococci detection in recreational waters. Improvements in research and instrument design that will eliminate high background and carryover may make this a viable technology in the     

Bipolar Disorder and Quality of Life: A Patient-Centered Perspective

Objectives: Several quantitative studies have now examined the relationship between quality of life (QoL) and bipolar disorder (BD) and have generally indicated that QoL is markedly impaired in patients with BD. However, little qualitative research has been conducted to better describe patients’ own experiences of how BD impacts upon life quality. We report here on a series of in-depth qualitative interviews we conducted as part of the item generation phase for a disease-specific scale to assess QoL in BD. Methods: We conducted 52 interviews with people with BD (n=35), their caregivers (n=5) and healthcare professionals (n=12) identified by both convenience and purposive sampling. Clinical characteristics of the affected sample ranged widely between individuals who had been clinically stable for several years through to inpatients who were recovering from a severe episode of depression or mania. Interviews were tape recorded, transcribed verbatim and analyzed thematically. Results: Although several interwoven themes emerged from the data, we chose to focus on 6 for the purposes of this paper: routine, independence, stigma and disclosure, identity, social support and spirituality. When asked to prioritize the areas they thought were most important in determining QoL, the majority of participants ranked social support as most important, followed by mental health. Conclusions: Findings indicate that there is a complex, multifaceted relationship between BD and QoL. Most of the affected individuals we interviewed reported that BD had a profoundly negative effect upon their life quality, particularly in the areas of education, vocation, financial functioning, and social and intimate relationships. However, some people also reported that having BD opened up new doors of opportunity.     

Assessment of the hepatitis B birth dose vaccination program,Papua New Guinea, 2014

IntroductionPapua New Guinea (PNG) implemented hepatitis B birth dose (BD) vaccination in 2005 yet since that time coverage has remained low, allowing mother-to-child transmission to occur. We conducted a field assessment of the BD vaccination program to develop strategies for improving the BD coverage.MethodsWe selected five provinces with higher hepatitis B prevalence and five with lower prevalence based on the results of a 2013 hepatitis B serological survey. Within each province, we interviewed district and provincial health officers, health workers, village volunteers, and caregivers from ten randomly selected health facilities. Data were collected on knowledge, practice, vaccine management and data recording/reporting. To identify enabling factors and barriers, we compared health facilities with higher BD coverage with those with lower coverage, and compared caregivers whose children received BD with those whose children did not.ResultsOverall timely BD coverage was 31% and BD vaccination was taking place in 81% of sampled health facilities. Lack of cold chain and vaccine were the major reasons for not providing the BD. Insufficiencies in supervision, vaccine management, community outreach, and data management were identified as obstacles to achieving high timely hepatitis B BD coverage. Good supervision, knowledge of hepatitis B and hepatitis B vaccination, antenatal care including information about the hepatitis B BD, provision of vaccine refrigerators in maternity wards, and outreach vaccination for home deliveries were associated with higher timely BD coverage.DiscussionSeveral steps will likely be effective in improving BD coverage: strengthening training and supervision among health workers and officers, educating caregivers on the benefits of the BD and delivery in health facilities, improving vaccine management, and improving data quality. Considerable effort and leadership will be needed to achieve these steps.