剖宫产与新生儿高胆红素血症的关系探讨

目的　探讨剖宫产分娩与新生儿高胆红素血症的关系。方法　对我院 2 0 0 0年 1月至 2 0 0 1年 9月期间在产科病房出生的新生儿进行微量血清胆红素测定 ,比较剖宫产娩出的新生儿与非剖宫产娩出的新生儿发生高胆红素血症的情况。结果　剖宫产组新生儿高胆红素血症的发生率为 39 6 % (4 70 / 1188) ,非剖宫产组新生儿高胆红素血症的发生率为 2 2 8%(2 4 5 / 10 74 ) ,两者差异有统计学意义 ,χ2 =73 2 ,P <0 0 5。剖宫产组中母亲有妊娠合并症者新生儿高胆发生率为 4 0 1%(10 0 / 2 4 9) ,母亲无妊娠合并症者新生儿高胆发生率为 39 4 % (370 / 939) ,两者差异无统计学意义 ,χ2 =0 0 4 7,P >0 0 5。结论　剖宫产可能是引起新生儿高胆红素血症的原因之一。     

新生儿高胆红素血症与临床脑电图关系探讨

目的：探讨新生儿高胆红素血症对脑电图的影响。方法：对284例高胆红素血症患儿在治疗前后分四次进行脑电图检查。结果：本组患儿在治疗前脑电图异常率52.5%,治疗结束后逐步降至6.3%;轻度高胆红素血症患儿治疗后中重度脑电图异常为4.0%,中度高胆红素血症患儿为7.2%,重度高胆红素血症患儿为9.0%;28-32 W高胆红素血症患儿经过治疗后脑电图异常率为9.5%,其他33-36W、37-40W、41-44W胎龄组脑电图异常依次为8.3%、5.8%、2.8%;经比较,高胆红素血症患儿脑电图异常的发生与胆红素水平呈正相关,与胎龄呈负相关。结论：新生儿高胆红素血症对脑电图影响很大,胎龄越小,脑电图异常率越大;中重度黄疸可以造成脑电图异常,轻度黄疸也可以造成中重度脑电图异常。     

370例新生儿高胆红素血症病因分析

目的探讨新生儿高胆红素血症在本地区病因分布况. 方法对新生儿高胆红素血症370例进行病因分析. 结果病因前五位的依次为:溶血症、感染、围产因素、肝炎综合症,母乳性黄胆. 结论高胆红素血症的病因,随地区,气候及当地医疗条件,经济状况,社会制度而不同.G-6PD缺陷是本地区高胆红素血症的首发病因,本地区是高发区 ,应常规筛查及预防.     

新生儿高胆红素血症MRI检查临床分析

目的探讨磁共振成像（MRI）技术协助诊断胆红素脑病的临床价值。方法回顾性分析2012年1月-2012年12月期间,对本院14例高胆红素血症患儿行头颅MRI检查的临床资料。结果高胆红素血症14例中,有胆红素脑病早期临床表现的5例,有痉挛期临床表现的1例,MRI异常者有5例（去除可疑1例）,均显示双侧苍白球T1WI信号及T2-FLAIR（抑水系列）呈对称性高信号,在T2WI呈正常信号,脑干听觉诱发电位（BAEP）异常有7例。结论 MRI能为早期高胆红素脑病提供客观指标,尤其对临床可疑病例的诊断方面有较高的临床价值。     

370例新生儿高胆红素血症病因分析

目的：探讨新生儿高胆红素血症在本地区病因分布况。方法：对新生儿高胆红素血症370例进行病因分析。结果：病因前五位的依次为：溶血症、感染、围产因素、肝炎综合症,母乳性黄胆,结论：高胆红素血症的病因,随地区,气候及当地医疗条件,经济状况,社会制度而不同,G－6PD缺陷是本地区高胆红素血症的首发病因,本地区是高发区,应常规筛查及预防。     

经皮胆红素测定仪在新生儿高胆红素血症中的临床应用

为了寻找一种方便，无创性的胆红素检测手段，我们采用经皮检测法，采用经皮黄疸仪，共测450名黄疸新生儿，结果发现经皮检测值与血清检测值存在高度直线正相关关系。且得出直线回归方程。结论经皮黄疸仪以其简便、安全的特点，可作为临床监测胆红素的一种手段，且可由经皮检测值推算出血清胆红素值。     

新生儿高胆红素血症甲状腺功能改变及意义

目的探讨新生儿高胆红素血症(简称高胆)对甲状腺功能的影响。方法对162例高胆新生儿采用竞争性放射免疫法进行血清甲状腺功能即三碘甲腺原氨酸(triiodothyronine,T3)、甲状腺素(thyroxine,T4)、游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(thyroid-stimulating hormone,TSH)测定,对照组参照文献结果。按黄疸程度分为轻度、中度、重度三组比较。结果轻、中度高胆组T4、FT3低于对照组(P<0.01),T3、FT4、TSH与对照组比较差异无显著性(P>0.05);重度组FT3、FT4、TSH低于对照组(P<0.05～0.01),T3、T4与对照组比较差异无统计学意义(P均>0.05)。高胆新生儿甲状腺功能T3、T4、FT3、FT4、TSH与TSB水平无明显相关性(P均>0.05)。结论新生儿高胆可引起甲状腺功能降低,T4和FT3降低可能是高胆新生儿甲状腺损害的敏感指标,FT4降低对预警病情严重有意义。     

脐血胆红素与新生儿黄疸的关系

我们对７８例足月，自然分娩，Ａｐｇａｒ评分７分以上的新生儿出生时记录胎龄，胎次，出生体重，胎儿出生后立即检测了脐血胆红素，Ｈｂ，白蛋白，血型，Ｃｏｏｍｂ‘ｓ试验。生后第３天采静脉血测定血清胆红素，根据生后黄疸出现与否分为黄疸组和非黄疸组。结果黄疸与非疸组婴儿在胎龄，胎闪，出生体重，脐血Ｈｂ，白蛋白方面两组均无显著差异；而脐血胆红素和血清胆红素浓度两组间差异显著。     

新生儿高胆红素血症病因与听力损害关系的探讨

目的　探讨新生儿高胆红素血症病因与听力损害之间的关系。方法　分别对不同病因 (溶血、感染、母乳性黄疸 )引起的高胆红素血症和正常新生儿进行脑干听觉诱发电位检测。结果　由溶血和感染因素所致的高胆红素血症脑干听觉诱发电位变化明显。母乳性黄疸所致高胆红素血症脑干听觉诱发电位无变化。结论　早期新生儿因溶血、感染所致高胆红素血症可引起听力损害 ,应及早干预 ,并注意远期随访。     

81例新生儿高胆红素血症的病因分析

我院93年1月至94年5月共收治81例高胆红血素血症患儿，占同期住病人的4．9％，81例黄疸病例均用经皮胆红素测定仪监测胆红素动态变化。其中46例接受光疗，光疗最长时间为72小时，最短时间为12小时，胆红素值由“34”降至“15”，平均下降“5－6”。19例轻型患儿进行日光浴。窒息、羊水吸入、早产儿、早产合并硬肿症、ABO溶血、Rh溶血等所引起的黄疸，临床表现轻重不一；针对不同病因，采取相对应的处理措施，可防止黄疸进一步加重，有利于优生优育。     

Evaluation of coagulation tests in mouse plasma

The coagulation variables thrombin time (TT), activated partial thrombin time (APTT) and prothrombin time (PT), were investigated in mouse plasma. TT and APTT clotting times were determined using a KC10 coagulation analyser and test kits Dia Thrombin (bovine thrombin diluted to a concentration of 2.5 U/l) and Dia Celin-L (rabbit brain cephaloplastin, activated with complexed Kaolin), respectively. PT was determined with IL Test™ Hepatocomplex (rabbit brain thromboplastin and calcium ions) using an ACL 200. Furthermore, the test procedures were also used to assess the anticoagulant status of mice treated orally with Melagatran, a thrombin inhibitor. The results showed that the kits could be used successfully on mouse plasma to measure the effect of a thrombin inhibitor on haemostasis.     

不同保存条件对PT和APTT结果的影响

目的 :探讨不同采血方式及保存条件对凝血酶原时间 (PT)和活化部分凝血活酶时间 (APTT)结果的影响。方法 :对我院正常体检人员在不同采血方式、不同温度、不同时间下测定的PT和APTT结果进行比较分析。结果 :血浆PT在 4℃保存 2h、2 4h的结果以及在2 5℃保存 2h、8h的结果与及时测定结果均无显著性差异 (P >0 .0 5 ) ;血浆APTT在 4℃保存 2h、8h的结果以及在 2 5℃保存 2h、4h的结果与及时测定结果均无显著性差异 (P >0 .0 5 )。在 3 7℃保存 2h后PT、APTT结果均出现显著性变化 (P <0 .0 5 )。两种采血方式比较 ,PT结果有显著性差异 (P <0 .0 5 )。结论 :凝血标本在室温( 2 5℃ )条件下 ,PT和APTT必须在 4h内完成检测 ,在 4℃条件下 ,必须 8h内完成检测 ;凝血标本抽血进程必须顺利且在 1min内完成     

Prothrombin times and activated partial thromboplastin times in toxicology: a comparison of different blood withdrawal sites for wistar rats

The prothrombin time (PT) and the activated partial thromboplastin time (APTT) for untreated male Wistar rats were determined on the Sysmex CA-5000 Instrument for blood taken from the orbital sinus, tail vein, vena cava and aorta. Boxplot and statistical analysis was performed. Only orbital sinus puncture yields unpredictable and unacceptable variation/prolongation of clotting times.     

Some species differences in the false prolongation of prothrombin times and activated partial thromboplastin times in toxicology

Changes in plasma activated partial thromboplastin times (APTT) and prothrombin times (PT) in mice, rats, rabbits, dogs, monkeys and human were examined for up to 96 h at storage temperatures of 4 and 25°C. Prolongation of APTT in rats was rapid and marked, with times doubling within 24 h post-sampling. Plasma APTT of human and monkey were also affected, but to a lesser extent. No effect was observed in mice, rabbits and dogs. On the other hand, the magnitude of PT changes was much smaller than that observed with APTT in all species. No significant differences were noted between the results from samples stored at 4°C or 25°C for either test.The false prolongation of APTT is clearly undesirable in a toxicity study, especially in rats. It is important therefore to minimise these changes by performing this test under strict time-controlled conditions.     

HLA-E基因与allo-HSCT移植效果关系的研究

目的： 探讨HLA-E基因与异基因造血干细胞移植（allo-HSCT）效果的关系。方法：用PCR-SSP方法分别检测22对allo-HSCT供者及受者的HLA-E基因型，并分2组：供受者HLA-E相合组和供受者HLA-E不合组。比较2组在移植后植入率、移植物抗宿主病（GVHD）、免疫重建、自体恢复或恶性病复发等方面的差异。结果：22对移植病例的供受者中，共检出3个HLA-E等位基因，分别为E*0101、E*01031和E*01032，未检出E*0102和*0104。供受者HLA-E相合组9对，供受者HLA-E不合组13对。两组在移植后植入率、GVHD、免疫重建、自体恢复或恶性病复发等无统计学差异。结论：HLA-E基因多态性与allo-HSCT移植效果未见相关性。     

Relationship between the result of preoperative pulmonary function test and postoperative pulmonary complications

Good preoperative screening and evaluation of patients undergoing surgery is necessary because it serves to identify the individual who is at risk of increased intra-operative and postoperative morbidity and mortality. The retrospective study was done in an attempt to determine if abnormalities in preoperative pulmonary function, detected by routine pulmonary function tests, would predict which patients would suffer from pulmonary complications following surgery. Pulmonary complications in the postoperative period included fever, atelectasis and respiratory failure. The overall incidence of pulmonary complications from our 78 patients undergoing surgery was 37 percent based on clinical criteria. This incidence was high in patients with FEV1 less than 1.0 L/sec, MVV less than 40% of predicted value and PCO2 more than 45 mmHg.     

Total IgE influences the relationship between skin test and RAST

Positive correlations were found between total IgE, skin prick test (SPT) and RAST results with tree pollen allergens. The relationship between SPT and RAST was influenced by the level of total IgE. At each level of atopy, defined according to the strength of SPT, patients with high total IgE had higher RAST values than patients with low total IgE.     

Analysis of the result of thyroid microsomal particle agglutination test by ELISA

No accurate method to detect thyroid microsomal (MC) antibody (Ab) in serum has been generalized. In this study, the titer of MC Ab obtained by the method of MC autoantibody particle agglutination (MCPA) was analyzed by enzyme linked immunosorbent assay (ELISA). MC and thyroglobulin (Tg) were prepared from Graves' thyroid. ELISA was done by coating the plate with MC, adding Tg to buffer and using peroxidase-conjugated anti-h IgG. 1) The titer of MCPA correlated with the MC Ab ELISA index in serum without Tg Ab, but it did not in serum with Tg Ab. MC Ab was negative by ELISA while it was positive by MCPA in some of the sera with Tg Ab. 2) When ELISA was done using buffer without Tg, the amount of IgG bound to MC was greater in serum with TGPA: + and MCPA: - than in serum with MCPA: + and TGPA: -. 3) The zone phenomenon observed in MCPA did not always indicate an excess of MC Ab. 4) MC Ab was positive by ELISA in some of the negative MCPA sera obtained from patients with Hashimoto's disease in which diagnosis was confirmed by biopsy. In conclusion, the result obtained in MCPA now in use is strongly influenced by Tg Ab. Furthermore, since binding of Ab to MC is judged by agglutination of particles in MCPA, ELISA is superior in sensitivity and accuracy in detecting MC Ab.