Non-taxane compounds from the bark of Taxus yunnanensis

From the bark of Taxus yunnanensis, 15 non-taxane compounds were isolated. Through spectroscopic methods such as ID and 2D NMR and MS experiments, one of them was determined as a new abietane-type diterpenoid named taxayunnin (1). The other 14 known compounds were identified as taxamairin C (2), taxamairin A (3), 3beta-hydroxy-sandaracopimaric acid (4), (+)-3-hydroxy-isodrimenin (5), rubrosterone (6), ponasterone A (7), ecdysterone (8), 20-hydroxy-echysone-20,22-monoacetonide (9), 7-oxositosterol (10), stigmast-4-en-6beta-ol-3-one (11), 5alpha,6beta-dihydroxy-daucosterol (12), beta-sitosterol (13), daucosterol (14), 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-N-(2'-hydroxypalmitoy])-octadeca-sphinga-4,8-dienine (15), respectively. Compounds 4-6, 9-12 and 15 were isolated from Taxus plants for the first time.     

Flavonoids from<Emphasis Type="Italic">Iris spuria</Emphasis> (Zeal) cultivated in Egypt

A new 12a-dehydrorotenoid 1, 11-dihydroxy-9, 10-methylenedioxy-12a-dehydrorotenoid (1), together with a new isoflavonoid glycoside tectorigenin-7-O-beta-glucosyl-4'-O-beta-glucoside (3), were isolated and identified from the rhizomes of I. spuria (Zeal). In addition, 4 known compounds, tectorigenin (2) tectorigenin-7-O-beta-glucosyl (1 --> 6) glucoside (4), tectoridin (a tectorigenin-7-O-beta-glucoside) (5) and tectorigenin-4'-O-beta-glucoside (6) were isolated and identified for the first time from this plant. The structures of the isolated compounds were determined by spectroscopic methods (UV, IR, 1H, 13C-NMR, DEPT, HMQC, NOESY, and HMBC experiments and MS spectrometry) and by comparison with literature data of known compounds. Compounds 2, 4, 5, and 6 are reported for the first time from this plant through the present study.     

A new coumaronochromone from Sophora japonica

A new coumaronochromone derivative, sophorophenolone ( 1 ), along with 13 known compounds, l -maackiain ( 2 ), medicagol ( 3 ), 7- O -methylpseudobaptigenin ( 4 ), pseudobaptigenin ( 5 ), 7,3'-di- O -methylorobol ( 6 ), genistein ( 7 ), prunetin ( 8 ), daidzein ( 9 ), formononetin ( 10 ), Di- O -methyldaidzein ( 11 ), quercetin ( 12 ), kaempferol ( 13 ) and isorhamnetin ( 14 ) were isolated from pericarps of Sophora japonica L. The structure of compound 1 was established by UV, IR, MS, and one-dimensional and two-dimensional NMR spectroscopy, including DEPT, NOESY, 1 H- 1 H COSY, HMQC, and HMBC experiments.     

Non-taxane compounds from the bark of Taxus yunnanensis

From the bark of Taxus yunnanensis , 15 non-taxane compounds were isolated. Through spectroscopic methods such as 1D and 2D NMR and MS experiments, one of them was determined as a new abietane-type diterpenoid named taxayunnin ( 1 ). The other 14 known compounds were identified as taxamairin C ( 2 ), taxamairin A ( 3 ), 3 g -hydroxy-sandaracopimaric acid ( 4 ), (+)-3-hydroxy-isodrimenin ( 5 ), rubrosterone ( 6 ), ponasterone A ( 7 ), ecdysterone ( 8 ), 20-hydroxy-echysone-20,22-monoacetonide ( 9 ), 7-oxositosterol ( 10 ), stigmast-4-en-6 g -ol-3-one ( 11 ), 5 f ,6 g -dihydroxy-daucosterol ( 12 ), g -sitosterol ( 13 ), daucosterol ( 14 ), 1- O - g - d -glucopyranosyl-(2 S , 3 R , 4 E , 8 Z )-2- N -(2'-hydroxypalmitoyl)-octadeca-sphinga-4,8-dienine ( 15 ), respectively. Compounds 4 - 6 , 9 - 12 and 15 were isolated from Taxus plants for the first time.     

山莓化学成分研究

目的对山莓的化学成分进行研究。方法采用硅胶柱色谱、Sephadex-20柱色谱、重结晶,并经高效液相色谱等技术提取分离山莓的化学成分,用UV、IR、EI-MS、ES-MS及NMR等光谱方法鉴定化合物结构。结果从山莓乙醇提取物的石油醚部位分离得到化合物1～6,分别鉴定为豆甾醇(1)、β-谷甾醇(2)、胡萝卜苷(3)、Homofukinolide(4)、Bakkenolide-B(5)、Bakkenolide-D(6);从二氯甲烷部位分离得到化合物7～10,分别鉴定为4-羟基-3-甲氧基苯甲酸(7)、芹菜素(8)、木樨草素(9)、山莓素(10)。结论化合物4、5、6、7、8、9首次从山莓子中分离得到,化合物10是新化合物。     

Chemical constituents from Matricaria chamomilla L. (I)

In the present study, we aimed to perform a phytochemical investigation of the whole herb of Matricaria chamomilla L., a Uygur herbal medicine. A total of 18 phenolic compounds were isolated by silica gel and Sephadex LH-20 chromatography, together with preparative HPLC methods. By analysis of the MS and NMR spectroscopic data and comparison with those in literature, these 18 compounds were identified as apigenin (1), galangin (2), luteolin (3), kempherol (4), quercetin (5), hispidulin (6), 6-methoxykaempferol (7), eupafolin (8), 3-methylquercetin (9), ermanin (10), 5,7,4′-trihydroxy-3,6-dimethoxyflavonone (11),bracteoside (12), 7-O-(β-D-glucopyranosyl)-galactin (13), isochlorogenic acid B (14), isochlorogenic acid C (15), 4-hydroxybenzoic acid (16), 5-pentadecylbenzene-1,3-diol (17) and scopoletin (18). Among them, compounds 1–13 were identified as flavonoids. Compounds 2 and 6–17 were isolated from both the plant and the Matricaria genus for the first time.     

板栗壳化学成分研究

从板栗壳95%乙醇浸膏中分离鉴定了16个化合物,分别是β-谷甾醇(1)、β-胡萝卜苷(2)、豆甾-5-烯-3β,7α-醇(3)、麦角甾-6,22-二烯-3β,5α,8α-三醇(4)、齐墩果酸(5)、豆甾-4-烯-6a-醇-3-酮(6)、豆甾-4-烯-6β-醇-3-酮(7)、原儿茶酸(8)、水杨酸(9)、豆甾-5-烯-3β-羟基-7-酮(10)、对羟基桂皮酸甲酯(11)、没食子酸(12)、绿原酸(13)、香(艹卓)醛(14)、乳糖(15)和芦丁(16).其中化合物3、6、9、10、11、13、14、15、16等9个化合物均为首次从该植物中分离得到,所有化合物均为首次从板栗壳中分离得到.     

地桃花化学成分研究.Ⅱ.酚酸类等化学成分

从地桃化伞草95%乙醇浸膏中分离鉴定了12个化合物,分别是丁香酸(1)、丁香酸葡萄糖苷(2)、水杨酸(3)、原儿茶酸(4)、原儿茶酸甲酯(5)、咖啡酸(6)、马来酸(7)、三十六碳酸 (8)、十五碳酸(9)、十六碳酸(10)、十七碳酸(11)、邻苯二甲酸异丁酯(12).除化合物7外,其余11个化合物均为首次从地桃花中得到,也是首次从梵天花属植物巾得到.     

Chemical constituents from the seeds of Capparis masaikai

A total of 21 compounds were isolated from the seeds of Capparis masaikai and identified as oxazolidine-2-thione (1), succinimide (2), catechol (3), octathiocane (4), monoethanolamine (5), 3-hydroxypropanenitrile (6), L-arabinose (7), 1,2,3-propanetriol (8), drummondol (9), spionoside B (10), adenosine (11), corchoionoside C (12), coniferin (13), syringin (14), cis-syringin (15), dihydrosyringin (16), indole-3-carboxylic acid (17), β-D-glucopyranosyl indole-3-carboxylic acid (18), 6-hydroxyindole-3-carboxylic acid β-D-glucopyranosyl ester (19), linoleis monoglyceride (20), and triolein (21). Their structures were identified based on physicochemical property and spectroscopic analysis, including MS, NMR, and single-crystal X-ray crystallographic data. Compounds 2–10 and 12–21 were isolated from C. masaikai for the first time.     

榼藤藤茎醋酸乙酯部位化学成分的研究

目的对榼藤Entada phaseoloide藤茎醋酸乙酯部位的化学成分进行研究。方法综合运用硅胶、反相硅胶RPC18、SephadexLH-20等色谱法进行分离,NMR、MS等波谱学方法进行结构鉴定。结果从榼藤藤茎甲醇提取物的醋酸乙酯萃取部位中分离得到了11个化合物,通过波谱分析鉴定为表没食子儿茶素没食子酸酯(1)、表儿茶素没食子酸酯(2)、落新妇苷(3)、木犀草素(4)、(?)-表儿茶素(5)、甘草苷(6)、β-香树脂醇(7)和日耳曼醇(8)的混合物、没食子酸(9)、β-谷甾醇(10)、胡萝卜苷(11)。结论所有化合物均为首次从榼藤中分离得到,其中化合物1～9在榼藤子属植物首次报道。     

大叶白蜡树皮的化学成分研究

目的:分离并鉴定大叶白蜡树皮的化学成分。方法:采用乙醇提取、硅胶柱色谱层析、制备薄层色谱等方法分离样品,所得单体通过紫外光谱、红外光谱、核磁共振及质谱等波谱分析法确定结构。结果:从样品中分得11个化合物,分别为咖啡酸(1)、丁香醛(2)、槲皮素(3)、槲皮素-3-O-β-D-葡萄糖苷(4)、木犀草素-7-O-β-D-葡萄糖苷(5)、黄芩素(6)、槲皮素-3-O-α-L-鼠李糖苷(7)、丁香苷(8)、木犀草素(9)、汉黄芩素(10)、芦丁(11)。其中化合物3、4、5、6、7、9、10均为首次从该属植物中分得。结论:本试验结果可为大叶白蜡树的进一步研究提供试验依据。     

中药五味子中活性物质的化学研究──五味子醇甲的全合成

本文以没食子酸为原料进行还原偶合及脱水反应生成顺、反1,4-二(3,4,5-三甲氧基苯基)-2,3-二甲基-2-丁烯化合物(7)。将其进行硼氢化反应,然后分别与2,3-二氯-5,6-二氰-1,4-苯醌(DDQ)和三氟醋酸铊(TTFA)在三氟醋酸(TFA)中进行非酚氧化偶合反应合成了中药五味子中活性化学成分五味子醇甲外消旋体,即(αRS)1,2,3,10,11,12-六甲氧基-6,7-二甲基-6-羟基-5,6,7,8-四氢二苯骈[a,c]环辛烯(9)。元素分析,MS,UV,IR及NMR光谱数据均与天然五味子醇甲相符。     

中药益智化学成分的研究

目的：研究中药益智Alpinia oxyphylla Miq.的化学成分。方法：利用各种色谱技术进行分离纯化,根据化合物的理化性质和光谱数据进行结构鉴定;用GC-MS联机技术鉴定挥发油的化学成分。结果：从益智分得10种化合物,其中2个二芳基庚酮:yakuchinone A (1)、益智醇(oxyphyllacinol 2),3个eremophilane烷碳架的倍半萜valencene (3)、nootkanone (4)、nootkanol (5),两个黄酮:杨芽黄酮(tectochrysin 6)、白杨素(chrysin 7),以及β-谷甾醇(8)、胡萝卜苷(9)和脂肪酸(10)。用GC-MS鉴定了挥发油中64个化学成分,其中含量较高的有:p-cymene (44.87%), valencene (9.13%), linalool (4.39%), myrtenal (3.90%), β-pinene (3.87%), α-pinene (2.93%), furopelargone (2.62%), terpinen-4-ol (2.56%)等。结论：益智醇(2)为新二芳基庚醇,其化学结构为1-(4′-hyroxy-3′-methoxy-phenyl)-7-phenyl-3-heptanol,3,6和7是首次从该植物分得。     

青钱柳地上部分的化学成分研究

从青钱柳Cyclocarya paliurus(Batal)ljinskaja.地上部分分离出14个化合物,通过NMR波谱方法和理化性质考察分别鉴定为山柰酚(1),山柰酚-3-O-α-L-吡喃鼠李糖苷(2),山柰酚-3-O-β-D-吡喃半乳糖苷(3),山柰酚-3-O-β-D-吡喃葡萄糖苷(4),山柰酚-3-O-α-L-(4′′-E-p-香豆酰基)吡喃鼠李糖苷(5),青钱柳酸B(6),阿江榄仁酸(7),2α,3β,23-三羟基-12-烯-28-乌苏酸(8),2α-羟基乌苏酸(9),齐墩果酸(10),槲皮素(11),异槲皮苷(12),β-谷甾醇(13),胡萝卜苷(14)。化合物2、3、5和8为首次从青钱柳属中分离得到。     

苏木的化学成分研究

研究苏木Caesalpinia sappan L．的化学成分。利用溶剂萃取，反复硅胶、凝胶柱色谱和反相硅胶等手段进行分离纯化，根据理化性质、波谱数据进行结构鉴定。分离并鉴定了14个化合物，分别为巴西苏木素（1），苏木酮B（2），（E）-3-（3，4-dihydroxybenzylidene）-7-hydroxychroman-4-one（3），3-去氧苏木酮B（4），brazilideA（5），1，7-二羟基[口山]酮（6），槲皮素（7），鼠李素（8），苏木查耳酮（9），3-去氧苏木查耳酮（10），butein（11），2,4，5-三羟基苯甲醛（12），3,8，9-trihydroxy-6H-benzo[c]chromen-6-one（13），β-谷甾醇（14）。化合物12和13为两个新天然产物，首次报道了化合物13的碳谱数据。化合物6为首次从豆科植物中分离得到，也是云实属分离得到的第一个[口山]酮类化合物。     

维药蜀葵花化学成分的分离与结构鉴定(Ⅰ)

目的扩大维药蜀葵花(Althaea rosea(Linn.)Cavan.)的药用资源。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱手段对蜀葵花进行分离纯化,并通过理化性质与波谱分析方法鉴定化合物的结构。结果从蜀葵花体积分数95%乙醇提取物中分离得到14个单体成分,分别鉴定为东莨菪内酯(scopoletin,1)、3,5,7-三羟基色原酮(3,5,7-trihydroxychromone,2)、反式咖啡酸(trans-caffeic acid,3)、4-hydroxy-3-methoxycinnamyl-β-D-glucopyranoside(4)、3,4-dime-thoxycinnamyl-β-D-glucopyranoside(5)、对羟基苯甲醛(p-hydroxybenzaldehyde,6)、对羟基苯甲酸(p-hydroxybenzoic acid,7)、4-O-β-D-吡喃葡萄糖氧基苯甲酸(4-O-β-D-glucopyranosylbenzoate,8)、对羟基苯甲酸-β-D-吡喃葡萄糖酯苷(p-hydroxybenzoyl-β-D-glucopyranoside,9)、4-methoxybenzyl-β-D-glucopyranoside(10)、原儿茶酸(protocatechuic acid,11)、3,5-二甲氧基-4-羟基苯甲酸(3,5-di-methoxy-4-hydroxy benzoic acid,12)、腺苷(adenosine,13)和1H-吲哚-3-羧酸(1H-indole-3-carboxylic acid,14)。结论化合物2、4～6、8～10和14为首次从蜀葵属中分离得到,3、11和13为首次从蜀葵中分离得到,并首次报道了化合物5的核磁共振波谱数据。     

钩吻根脂溶性化学成分的分离与鉴定

目的对钩吻根脂溶性部位的化学成分进行研究。方法通过乙醇回流提取、溶剂分步萃取得到钩吻根粗提物,利用硅胶柱、ODS柱、Sephadex LH-20、Toyopearl HW-40F等多种色谱柱进行分离纯化,利用NMR等现代波谱技术对分离得到的化合物进行结构鉴定。结果分离得到10个化合物,分别鉴定为钩吻素子(koumine,1)、钩吻绿碱(gelsevirine,2)、胡蔓藤碱乙(humantenine,3)、钩吻内酰胺(gelsemamide,4)、12β-羟基-孕甾-4,16-二烯-3,20-二酮(12β-hydroxy-pregna-4,16-diene-3,20-dione,5)、正三十一烷醇(n-hentriacontyl alcohol,6)、十四碳酸甲酯(methyl myristate,7)、6,7-二甲氧基香豆素(6,7-dimethoxycoumarin,8)、3,4,5-三甲氧基苯甲醇(3,4,5-trimethoxybenzyl alcohol,9)、5-甲基间苯二酚(5-methylresorcinol,10)。结论化合物6-10为首次从胡蔓藤属中分离得到。     

Lipid peroxidation inhibitory activity of some constituents isolated from the stem bark ofEucalyptus globulus

Twelve compounds with lipid peroxidation inhibitory activity were isolated from the stem bark of E. globulus. Their structures were assigned as a new aromatic monoterpene (1) and eleven known compounds, pinoresinol (2), vomifoliol (3), 3,4,5-trimethoxyphenol 1-O-beta-D-(6'-O-galloyl)glucopyranoside (4), methyl gallate (5), rhamnazin (6), rhamnetin (7), eriodictyol (8), quercetin (9), taxifolin (10), engelitin (11), and catechin (12) on the basis of UV, mass, and NMR spectroscopic analyses. These compounds except vomifoliol significantly inhibited lipid peroxidation in rat liver microsome.     

Labdane diterpenoids and shikimic acid derivatives from Araucaria cunninghamii

Four new (1-4) and two known compounds (5, 6) were isolated from the aerial parts of Araucaria cunninghamii. By analysis of UV, IR, MS, and NMR, the new compounds were identified as ENT-19-(Z)-coumaroyloxylabda-8(17),13(16),14-triene (1), ENT-19-( E)-coumaroyloxylabda-8(17),13(16),14-triene (2), shikimic acid N-butyl ester (3), and 5-(Z)-coumaroyloxyquinic acid N-butyl ester (4). Compounds 1 and 6 exhibited inhibitory activity against Escherichia coli, and compound 1 also exhibited moderate activity against human leukemia cells (HL-60) and human hepatoma cells (SMMC-7721) with IC?? values of 8.90 and 11.53 μM, respectively.     

Two new phenolic acids from Drynariae rhizoma

To study the chemical constituents of Drynariae Rhizoma, nine phenolic acids were isolated from a 70% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. By spectroscopic techniques including 1H NMR, 13C NMR, 2D NMR, and HR-ESI-MS, these compounds were identified as 4, 4'-dihydroxy-3, 3'-imino-di-benzoic acid (1), protocatechuic acid (2), gallic acid (3), p-hydroxybenzoic acid (4), (E)-caffeic acid (5), ethyl trans-3, 4-dihydroxycinnamate (6), caffeic acid 4-O-beta-D-glucopyranoside (7), p-coumaric acid 4-O-beta-D-glucopyranoside (8), and 23(S)-12-O-caffeoyl-12-hydroxyllauric acid glycerol ester (9), separately. Among them, 1 and 9 are new compounds, and 3, 4, and 6 were isolated from Drynaria species for the first time.