慢性牙周炎患者中间普氏菌临床分离株中溶血基因的分布

目的：研究溶血基因在慢性成人牙周炎患者中间普氏菌临床分离株中的分布状况，探讨溶血基因与中间普氏菌致病性的关系。方法：收集符合纳入标准的57例慢性成人牙周炎病人的牙周袋内的朗下菌斑，采用厌氧培养技术分纯产黑色素的革兰氏阴性厌氧杆菌，用16SrRNA聚合酶链反应(PCR)方法鉴定为中间普氏菌的菌株，保存并用溶血基因的相应引物检测该基因。结果：57例慢性成人牙周炎患者中，20例捡出中间普氏菌，检出率为35．1％。35株中间普氏菌的临床分离株中，5株能够检测到溶血基因，另外30株不能检测到溶血基因。结论：溶血基因在中间普氏菌临床分离株中呈多态分布。     

中间普氏菌在慢性牙周炎发病中的作用

对中间普氏菌在慢性牙周炎发病中的作用机制进行了综述.中间普氏菌通过向牙周组织黏附和定植、直接侵袭口腔组织细胞、促进炎性细胞因子的释放、干扰宿主免疫反应、促进骨吸收、抑制骨形成等作用而参与牙周炎的发生发展.     

DGGE分析中间普雷沃菌在原发性根尖周感染中的基因多态性

目的：检测中间普雷沃菌在原发性根尖周感染中的基因多态性。方法：收集17例原发性根尖周感染根管内的细菌学样本,针对其16 S rDNA V3?V6区段进行PCR?DGGE,割胶测序,序列比对。结果：中间普雷沃菌在原发性根尖周感染中的检出率为47?06％。 DGGE图谱中显示14条不同位置的条带,割胶测序后发现有3个不同的基因型。结论：通过PCR?DGGE证实了中间普雷沃菌在原发性根尖周感染中存在基因多态性。     

中间普氏菌菌体蛋白抗原研究

中间普氏菌菌体蛋白抗原研究华西医科大学口腔医学院口腔内科（６１００４１）潘亚萍，张蕴惠，肖卓然　　　　　　　　　　华西医科大学口腔医学院中心实验室章锦才，张萍，肖晓蓉中间普氏菌（Ｐｒｅｖｏｔｅｌｌａｉｎｔｅｒｍｅｄｉａ）原称中间类杆菌（Ｂａｃｔｅｒｏ...     

中间普氏菌内毒素刺激人牙周膜细胞上调表达基因的研究

目的：用基因芯片研究中间普氏菌内毒素刺激前后，牙周膜细胞上调表达基因。方法：应用点样数512点的基因芯片分析中间普氏菌内毒素刺激人牙周膜细胞上调表达基因。结果：研究发现中间普氏菌内毒素刺激牙周膜细胞后有6条上调基因，其中部分基因与蛋白激酶和蛋白磷酸酶有关。结论：牙周致病菌内毒素刺激牙周膜细胞后，可引起部分基因表达上调，从而影响牙周膜细胞正常的生理功能。     

牙龈卟啉菌、中间普氏菌的分离、培养和鉴定

目的：采用厌氧培养和鉴定技术，分离牙周炎患者龈下菌斑中的牙龈卟啉菌和中间普氏菌。方法：采集牙周炎患者的龈下菌斑，厌氧培养，分离产黑色素菌。经长波长紫外灯观察，各种生化分析和间接免疫荧光染色，分离和鉴定牙龈卟啉菌，中间普氏菌。结果：在受检的33例牙周炎患者中，24例患者检出了牙龈卟啉菌，总检出率为72．77％，共分离了79株。18例患者检出了中间普氏菌，总检出率为54．54％。共分离了32株。结论：厌氧培养，生化反应鉴定技术的发展，使牙龈卟啉菌和中间普氏菌的分离与培养准确可靠，为今后在分子水平上了解各菌株的致病机理打下了基础。     

中间普氏菌内毒素刺激前后下调人牙周膜细胞表达基因的研究

目的 :研究中间普氏菌内毒素刺激前后牙周膜细胞下调表达基因。方法 :应用点样数 5 12点的基因芯片分析中间普氏菌内毒素刺激前后牙周膜细胞下调表达基因。结果 :研究发现中间普氏菌内毒素刺激牙周膜细胞后 8条下调基因 ,包括转录调控基因、凋亡相关基因和受体基因。结论 :中间普氏菌内毒素刺激牙周膜细胞后 ,可引起部分基因表达下调 ,从而影响牙周膜细胞正常的生理功能     

人工种植牙龈下中间普氏菌1年内数量变化研究

目的研究人工种植牙龈下中间普氏菌（Prevotella intermedia,Pi）1年内的数量变化情况,为种植体的定期维护提供理论基础。方法于2010年6月到2011年6月,对在山西医科大学第一医院口腔科门诊已完成固定义齿修复的38例患者的46颗人工种植牙进行为期1年的复诊检查,记录修复后1、3、6、12个月4个时段,所有种植牙的菌斑指数（PLI）、牙龈指数（GI）、牙龈出血指数（GBI）、探诊深度（PD）以及X线骨吸收情况,并采用只的选择性培养基对龈下菌斑标本进行分离培养,计算每毫升标本原液的菌落形成单位（CFU／mL）。结果随时间延长,种植牙周咸检出量不断增加,1个月到3个月时增加的趋势最为明显,到6个月左右趋于稳定,其统计值在1个月和3个月、3个月和6个月间比较,差异有统计学意义（P〈0．05）,但在6个月和12个月间比较,差异无统计学意义（P〉0．05）。PLI、GI和X线骨吸收值在修复后1、3、6、12个月比较,差异均有统计学意义（P〈0．05）。GBI和PD仅在1个月和3个月间比较,差异有统计学意义（P〈0．05）,其余各时段间比较,差异均无统计学意义（P〉0．05）。结论在临床工作中,应注意加强修复后3个月时种植患者的口腔维护,防止种植体周围炎的发生,提高种植成功率。     

种植体周围袋中中间普氏菌的检测及临床意义

目的:研究一年内人工种植牙龈下中间普氏菌的变化情况,为种植体的定期维护提供理论基础。方法:临床选取38名种植患者的46枚种植体,并将其分成A、B两组。A组是种植修复后1年时探诊种植牙周有出血的种植体20枚,B组是无探诊出血的种植体26枚。检查记录种植修复后1个月,修复后3个月,修复后6个月,修复后12个月四个时段,入选种植牙的菌斑指数(PL)I,牙龈指数(G)I,探诊深度(PD),X-线骨吸收值;采用中间普氏菌的选择性培养基对种植体龈下菌斑标本进行了分离培养。结果:横向比较A、B两组之间中间普氏菌计数、牙龈指数(G)I、探诊深度(PD)有统计学差异,其余检测指标无统计学差别。修复后3月时,A、B两组之间中间普氏菌计数值有统计学差异。纵向比较,随时间延长,A、B两组种植牙周中间普氏菌检出量不断增加,1月到3月时增加的趋势最为明显,到6个月左右趋于稳定。其统计值在1个月和3个月比较有统计学差异,但在6个月和12个月比较无统计学差异。各临床指标和X-线指标随时间的变化无统计学意义。结论:在临床工作中,应注意加强修复后3月时种植患者的口腔维护,防止种植体周围炎的发生,提高种植成功率。     

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目的应用聚合酶链式反应-变形梯度凝胶电泳（PCR—DGGE）技术检测慢性根尖周炎患牙根管内中间普菌和链球菌定植情况,分析根管内细菌与患牙症状间关系。方法2011年12月至2013年5月于北京大学深圳医院口腔科就诊的27例慢性根尖周炎患牙根管内细菌样本,提取DNA,利用16SrDNA引物进行PCR—DGGE技术分析。结果27例共检出细菌菌属17种。中间普菌在17例有症状组中检测出16例（94．1％）,在10例无症状组中检测出6例（60．0％）,两组检出率差异有统计学意义（P〈0．05）。链球菌在17例有症状组中均未检测出,在10例无症状组中检测出4例（40．0％）,两组检出率差异有统计学意义（P〈0．05）。结论慢性根尖周炎患牙以厌氧菌感染为主,根管内中间普菌、链球菌与临床症状相关。     

2种糊剂对慢性根尖炎患牙侧副根管封闭性的效果比较

目的:观察2 种根管充填糊剂充填慢性根尖炎侧副根管的治疗效果.方法:选择术后X 线片显示有侧副根管的慢性根尖炎病例54 例, 随机分为AH-plus 组和赛普敦组,每组27 例.开髓、揭顶、去除牙本质肩领,应用逐步后退法进行根管预备,氢氧化钙糊剂根管封药消毒,分别选用AH-plus 糊剂和赛普敦(SEPTODONT)美松根管充填糊剂, 热牙胶连续波垂直加压充填技术进行根管充填.比较根尖1/3侧副根管充填率、治疗一年后根管治疗成功率和感染控制率.结果:AH-plus 组根尖1/3 侧副根管充填率为62.96%,根管治疗成功率为88.89%,感染控制率为96.30%,分别高于赛普敦组的37.04%、66.67% 和74.07%,差异有统计学意义(P<0.05).结论:AH-plus 糊剂较赛普敦美松糊剂对侧副根管充填的疗效好.     

中间普里沃菌基因多态性及其在成人牙周炎患者龄下菌斑中的分布

目的：了解中间普里沃菌（P．i)的基因多态性及其在成人牙周炎（AP）病变部位与健康部位的分布特点。方法：采用随机引物多聚酶链扩增法（APPCR）用法引物OPA－03和OPA－13对来自25例AP的101株P．i作基因型分析。结果：用引物OPA－03对101株P.i的APPCR分析,得到7种不同的基因型,用引物OPA－13对97株P.i的APPCR分析,得到12种不同的的基因型;同一患者口内有1－4种基因型,以1种为主,同一患者病变部位与健康部位均可有1－3种基因型,以1种为主;P.i在病变部位的优势基因型与P.i在健康部位的优势基因型基本一致,未发现与牙周健康状态相关的特定基因型P.i。结论：中间普里沃菌里内源性致病菌,在适宜的条件下过度生长导致牙周炎。     

Effect of different root canal sealers on periapical repair of teeth with chronic periradicular periodontitis

Teeth with induced chronic periradicular periodontitis in dogs were root canal treated. After the biomechanical preparation, using K files and 5.25% sodium hypochlorite as the irrigant solution, all root canals were dressed with an antibacterial dressing based on calcium hydroxide, which was left in place for 7 days. After this time, the root canals were obturated with lateral condensation of cold gutta-percha with either a calcium hydroxide root canal filling material (Sealapex) or a zinc oxide-eugenol sealer (Fill Canal). After 270 days, histopathological analysis showed better apical and periapical repair in the teeth obturated with Sealapex ( P < 0.05).     

比塔派克斯糊剂治疗感染根管的临床观察

目的：观察比塔派克斯（Vitapex）糊剂在感染根管治疗中疗效。方法：选择患慢性根周炎的恒牙159个,随机分为3组,分别充填Vitapex糊剂、氧化锌丁香油糊剂及氧化锌丁香油加碘仿糊剂,观察其临床近期疗效。结果：Vitapex糊剂充填组术后反应明显轻于其它两组（P＜0.005）,超充糊剂3个月复查时吸收率高于其它两组（P＜0.01）,而一年愈合率3组间无明显差别（P＞0.05）。结论：Vitapex糊剂应用于感染根管的充填近期疗效优于氧化锌丁香油糊剂,而一年复查效果无明显差别。     

Detection of Prevotella intermedia in subgingival plaque of adult periodontitis patients by polymerase chain reaction

A PCR assay was developed that could specifically amplify DNA from the periodontal pathogen Prevotella intermedia. A pair of primers was selected from regions of the 16S rRNA gene of P. intermedia that were both divergent in sequence at their 3' ends with respect to the corresponding regions of the 16S rRNA gene of P. nigreseens , its most closely related species, and used in the PCR assay. Positivity was indicated by amplification of an 855 bp product. Using purified genomic DNA from these 2 species, assay conditions were determined under which only P. intermedia DNA and not P. nigrescens DNA was amplifiable. Absolute specificity of the assay was confirmed by the fact that no amplification products were obtained when using DNA from several other important periodontal organisms. The optimized PCR assay was used to identify P. intermedia in subgingival plaque samples of patients with adult periodontitis. Confirmation of amplification of P. intermedia DNA was achieved by digestion of PCR products with the restriction endonuclease R sal, which gives different restriction patterns for P. intermedia and P. nigrescens. Of the 97 samples analysed, 38 (39%) were positive for P. intermedia. The results obtained confirm P. intermedia as a possible aetiological agent of adult periodontitis. Additionally, PCR primers targeting the corresponding region of the 16S rRNA gene of P. nigrescens were shown to be specific for the organism when used in a PCR assay, although P. nigrescens was not detectable in any of the subgingival plaques analysed.     

Detection of Prevotella intermedia in subgingival plaque of adult periodontitis patients by polymerase chain reaction

A PCR assay was developed that could specifically amplify DNA from the periodontal pathogen Prevotella intermedia. A pair of primers was selected from regions of the 16S rRNA gene of P. intermedia that were both divergent in sequence at their 3′ ends with respect to the corresponding regions of the 16S rRNA gene of P. nigrescens, its most closely related species, and used in the PCR assay. Positivity was indicated by amplification of an 855 bp product. Using purified genomic DNA from these 2 species, assay conditions were determined under which only P. intermedia DNA and not P. nigrescens DNA was amplifiable. Absolute specificity of the assay was confirmed by the fact that no amplification products were obtained when using DNA from several other important periodontal organisms. The optimized PCR assay was used to identify P. intermedia in subgingival plaque samples of patients with adult periodontitis. Confirmation of amplification of P. intermedia DNA was achieved by digestion of PCR products with the restriction endonuclease Rsal, which gives different restriction patterns for P. intermedia and P. nigrescens. Of the 97 samples analysed, 38 (39%) were positive for P. intermedia. The results obtained confirm P. intermedia as a possible aetiological agent of adult periodontitis. Additionally, PCR primers targeting the corresponding region of the 16S rRNA gene of P. nigrescens were shown to be specific for the organism when used in a PCR assay, although P. nigrescens was not detectable in any of the subgingival plaques analysed.     

Investigation of the interleukin-1 gene cluster polymorphisms in Jordanian patients with chronic and aggressive periodontitis

Objective

Interleukin-1 (IL-1) is a proinflammatory cytokine that is highly elevated in response to bacterial biofilms and is a potential risk factor for periodontal diseases. IL-1 gene polymorphisms have been associated with the IL-1 level. The aim of this study was to investigate if IL-1 gene cluster polymorphisms are associated with chronic (CP) and aggressive (AgP) periodontitis in a Jordanian population.

Methods

A total of 100 CP, 80 AgP patients and 80 controls were genotyped using PCR for IL-1RN-86-bp VNTR and PCR-RFLP for IL-1A-889, IL-1B-511, -35, +3953, and IL-1RN +8006, +9589, +11100 SNPs. The distribution of alleles and genotypes between groups was compared using χ² analysis. Estimation of haplotype frequencies was carried out using the EH programme.

Results

The IL-1RN8006 SNP and the IL-1RN-VNTR were associated with CP but not with AgP. The C allele and TC genotype of IL-1RN8006 were increased in CP (P_corr = 0.002, 0.00026 respectively). The A1 allele and A1/A1 genotype of the IL1-RN-VNTR were significantly increased in CP (P_corr = 0.0007, <0.0001 respectively). The CA1 haplotype formed by both markers was present in 29 CP patients but not in any of the controls (P < 0.0001). No significant differences were found in the distribution of allele and genotype frequencies of the other markers between CP and AgP cases and controls.

Conclusions

IL-1RN 8006 and IL-1RN VNTR were associated with CP but not AgP in a Jordanian population, whilst other investigated markers in IL-1A, IL-1B and IL-1RN were not associated with either CP or AgP.     

中间普氏菌在家庭成员牙周菌斑中的分布调查

目的：调查了解中间普氏菌在牙周健康人群中的分布状况。方法：收集符合纳入标准的６０个家庭、１８１例受试者的牙周颈缘龈上菌斑和龈下菌班,采用厌氧菌培养获得２７９株产黑色素的Ｇ＾－厌氧杆菌,然后进行产黑色素的Ｇ＾－厌氧杆菌的纯化培养及微量生化鉴定。结果：中间普氏菌在牙周较健康的父母和儿童牙周菌班中均可检出,儿童群体中中间普氏菌阳性率达７０．４９％,而成人群体中中间普氏菌阳性率为４３．３３％,二者有显著性