Changes in phospholipid composition of mitochondria in the medulla oblongata and frontal lobes of the cerebral hemispheres in hemorrhagic shock in cats

Considerable regional differences in the phospholipid composition of mitochondrial membranes are found in the brain of cats in the terminal phase of hemorrhagic shock. The most prominent alteration is noted in the medulla oblongata and consists in a progressive elimination of phosphatidylcholine. Changes in the main phospholipids in mitochondrial membranes of the cerebral hemispheres are less pronounced and consist in a drop of phosphatidylinositol and phosphatidylethanolamine. Accumulation of lysophosphatidylcholine and lysophosphatidylethanolamine is a regular feature of the studied mitochondria. Accumulation of lysophosphatidylserine is found primarily in mitochondrial membranes of the medulla oblongata. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, No. 4, pp. 387–391, April, 1996     

Changes in the phospholipid composition of cat hepatocyte plasma membrane in hemorrhagic shock

Progressive elimination of phosphatidylcholine from the hepatocyte plasma membranes is observed during the development of hemorrhagic shock in cats. The phosphatidylinositol content decreases 30 min after the start of bleeding and then gradually increases. The phosphatidylethanolamine content is increased on the 30th and 60th min of bleeding, while the content of phosphatidylserine increases 1 h after the start of bleeding. At the peak of hemorrhagic shock the major shifts are observed in the phosphatidylcholine and phosphatidylinositol contents. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 3, pp. 261–263, March, 1997.     

Dose-dependent influence of buprenorphine on the phospholipid composition of cat hepatocyte plasma membranes in hemorrhagic shock

It is established that buprenorphine (0.3 mg/kg) induces considerable alterations in the phospholipid composition of hepatocyte plasma membranes as a result of phosphatidylserine accumulation and a considerable loss of sphingomyelin and lysophosphatidylserine. When administered in a dose of 0.03 mg/kg, buprenorphine facilitates normalization of the phosphoinositol turnover in hepatocyte plasma membranes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 8, pp. 164–165, August, 1994 Presented by G. N. Kryzhanovskii, Member of the Russian Academy of Medical Sciences     

Protective effect of phosphatidylcholine liposomes in cats with hemorrhagic shock

Experiments on cats indicated that the use of phosphatidylcholine liposomes in hemorrhagic shock may reduce the intensity of free-radical processes in the liver, stabilize the phospholipid bilayer of plasma membranes in hepatocytes, decrease the severity of pathomorphological changes in the target organs, and raise systemic arterial pressure with its stabilization at a subnormal level. The use of phosphatidylcholine liposomes in cats with hemorrhagic shock resulted in a considerable prolongation of their survival. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 5, pp. 480–484, May, 1995     

Changes in hemorheologic indices in the course of hemorrhagic shock

Relations between rheologic, hemodynamic, and metabolic indices during hemorrhagic shock were studied in experiments on dogs. The mechanical properties of the blood were found to change immediately after blood loss parallel with the macrohemodynamic disorders, and they preceded the development of metabolic acidosis. It is concluded that determination of the blood fluidity threshold can be used to establish the degree of the circulatory disturbance in shock.Department of Operative Surgery and Department of Theoretical and Experimental Physics, N. I. Pirogov Second Moscow Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR V. V. Kupriyanov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 81, No. 3, pp. 272–274, March, 1976.     

Effect of peptidergic correction of hemorrhagic shock on the behavior of rats in the posthemorrhagic period

Hemorrhagic shock is shown to disturb the behavior of rats in the open field test. Infusion of a mixture of thyrotropin releasing hormone and FMRFa (Phe-Met-Arg-Phe amide) in subthreshold doses led to a partial recovery of the neurological status. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 12, pp. 623–625, December, 1995 Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences     

Changes in rat liver mitochondria after bilateral subdiaphragmatic vagotomy

Changes in the liver mitochondria of rats after bilateral subdiaphragmatic vagotomy were studied. Two stages were distinguished in the dynamics of the response of the mitochondrial system to denervation. During the first stage (0.5–3 days after vagotomy) reversible functional disturbances due to postoperative stress took place in the mitochondria. The second stage (7–60 days after denervation) is characterized by more marked structural and functional changes with some common features with those observed in hypoxia and resulting from vagotomy itself.Department of Histology, Therapeutic Faculty, N. I. Pirogov Second Moscow Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR S. S. Debov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 4, pp. 420–422, April, 1977.     

Influence of hemorrhagic shock on learning activity in the late posthemorrhagic period

Anxiety and learning activity (acquisition of the conditioned behavior reaction trained with a bonus) were investigated in rats during 4 weeks after hemorrhagic shock. Some phasic behavioral alterations have been found in animals undergone hemorrhagic shock. Translated fromByulleten' Eksperimental'noi Biologii I Meditsiny, Vol. 125, No. 2, pp. 130–133, February, 1998     

Role of nitrogen metabolism of the brain in the mechanisms of the therapeutic action of hyperbaric oxygen in hemorrhagic shock

Hyperbaric oxygen therapy (3 atm O₂, 60 min) of hemorrhagic shock in cats (blood pressure 60/50 mm Hg) prevents the sharp increase, characteristic of hypoxia, in the concentration of ammonia, urea, alanine, and -aminobutyric acid in the brain and activates glutamine formation. In the sensomotor cortex the processes of ammonia detoxication via the pathway of increased synthesis of glutamate and glutamine take place more intensively than in the limbic structures of the brain.Department of Pathological Physiology, Voronezh Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR A. M. Chernukh.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 4, pp. 418–420, April, 1977.     

山莨菪碱对失血性休克肝线粒体内镁含量的影响

为探讨654-2抗休克的细胞机制,本工作在家兔晚期失血性休克模型上,观察654-2对休克动物肝细胞线粒体镁含量改变的影响,结果发现,失血性休克动物血浆镁浓度进行性升高与动物血压的降低呈负相关关系;休克动物肝线粒体镁含量明显减少;而用654-2治疗时,显著抑制失血性休克时血浆镁和肝线粒体镁含量的改变,明显改善休克动物的预后。实验结果提示654-2可能具有稳定休克动物肝线粒体的作用。     

Effect of intraperitoneal spermin on oxidative processes in isolated mitochondria of rat liver in hypothermia

Effect of intraperitoneal administration of spermin on oxidative phosphorylation and calcium capacity of isolated liver mitochondria was studied in normo- and hypothermic rats. Hypothermia stimulates mitochondrial respiration without decreasing the contingency and increases calcium capacity. Spermin suppresses mitochondrial respiration, the effect being stronger in hypothermia. In high doses spermin prevents stimulating effect of hypothermia on respiration and reduces the increase in calcium capacity. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 5, pp. 526–528, May, 1998     

Effects of apolipoproteins C on oxidative phosphorylation in rat liver mitochondria

It is shown that apoC-III, but not other apoC proteins, components of very low density lipoproteins (apoC-I, apoC-II, apoC-III), reduced the rate of mitochondrial respiration in various metabolic states. This effect depended on the dose of apoprotein, type of oxidized substrate, and the presence of Ca ions in the incubation medium. ApoC-III completely blocked oxidative phosphorylation during oxidation of palmitoyl carnitine by mitochondria, while the respiration rate in metabolic state 4 remained unchanged. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 8, pp. 184–186, August, 2000     

Phospholipid spectrum and lipid peroxidation in cell membranes of brain tissue in thymectomized rats

Lipid peroxidation (LPO) processes and the phospholipid (PL) content and composition in the homogenate and in the mitochondrial and supramitochondrial compartments of brain cells of adult male rats were studied 3 and 6 months after thymectomy. Thymectomy was found to markedly increase the rate of LPO and to reduce antiradical activity in all cellular compartments. A reliable increase of the level of total phospholipids in the homogenate and mitochondria was observed by the third month after thymectomy and a reduction of its control levels during the sixth month postoperation. On the other hand, a reduction of the content of total phospholipids was observed in the supramitochondrial fraction by the sixth month of the experiment. An increase of the phosphatidylethanolamine (PE) and phosphatidylserine (PS) contents was found to be mainly responsible for the changes in the level of total phospholipids. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 22–24, July, 1994 Presented by E. D. Gol'dberg, Member of the Russian Academy of Medical Sciences     

Functional state of the left ventricle in cardiocytotoxic shock

Changes in the cardiodynamics and contractile function of the myocardium were studied in experimental shock arising in 20 anesthetized dogs in response to intracoronary injection of anticardiac cytotoxic serum. Besides signs of a disturbance of left ventricular function (a fall in the systolic index, the working stroke index of the left ventricle, the rate of rise of intraventricular pressure, the indices of myocardial contractility, and the ejection fraction) a decrease was observed in the end-diastolic volume and pressure in the left ventricle. These results indicate that disturbances of the cardiodynamics are due to both cytotoxic injury to the heart and a disturbance of the blood flow to the left heart.Department of Experimental Cardiology, A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. (Presented by Academician of the Academy of Medical Sciences of the USSR N. N. Gorev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 82, No. 11, pp. 1291–1294, November, 1976.     

Effect of doxorubicin on oxidative phosphorylation in brain mitochondria

Inin vitro experiments, antineoplastic anthracycline antibiotic doxorubicine caused deenergization of rat brain mitochondria due to oxidation-phosphorylation uncoupling and inhibition of succinate oxidase system. Reduced production and oxidation of succinic acid is the most early effect of doxorubicin on mitochondrial oxidation. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 445–447, October, 1999     

Protective effect of adaptation to stress against the hemorrhagic shock-induced damage: the role of the antioxidant system

Modeling of hemorrhagic shock in rats adapted to immobilization stress required the removal of greater volumes of blood than that in control rats. The antioxidant system activation in adapted rats was accompanied by an increase in resistance to blood loss. The antishock effect of preliminary adaptation to stress was shown for the first time. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 9, pp. 270–273, September, 1998     

Mechanism of disturbances of energy formation in acute ischemia of the liver

Respiration of rat liver mitochondria after the addition of ischemic toxin to the incubation medium was compared with respiration of mitochondria isolated from ischemic rat liver. The changes in respiration in both cases could be prevented by preliminary addition of dithiothreitol and reduced by subsequent addition of cytochrome c or dithiothreitol to the incubation medium. The similarity between the mechanisms of disturbance of energy formation is postulated.Laboratory of Transplantation of Organs and Tissues, Academy of Medical Sciences of the USSR, Moscow. Institute of Clinical and Experimental Medicine, Academy of Medical Sciences of the USSR, Siberian Branch, Novosibirsk. (Presented by Academician of the Academy of Medical Sciences of the USSR V. V. Kovanov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 9, pp. 301–303, September, 1977.     

Mechanisms of reciprocal control of reactions and processes involved in energy production by mitochondria

Correlations between respiration rates in the mitochondria in different states are studied using various oxidation substrates. Specific features and integration between different functional cycles are substrate-dependent. It is suggested that variations of the mitochondrial function correspond to specific phases of pathological process. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 9, pp. 282–284, September, 1996     

Comparative structural analysis of the liver and kidneys in experimental renal failure

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 5, pp. 546–548, May, 1993     

Effect of dexamethasone on the composition of skin phospholipids in laboratory animals

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 378–380, April, 1993