股骨头坏死的介入治疗

目的：探讨股骨头缺血性坏死（ANFH）的介入治疗效果。方法：对12例ANFH患者的12髋先常规行股动脉DSA,后在股骨头的供血动脉旋股内、外侧动脉内灌注溶栓、扩血管药物,即在每支血管内注入稀释后的罂粟碱25mg、尿激酶25万u、低分子右旋糖酐80mL、血栓通0.15mg;术后每日静脉滴注低分子右旋糖酐500mL、血栓通0.3mg及肝素钠4500 u,连用5d,治疗后行股骨头X线平片及/或CT随访半年以评估疗效。结果：术后患者患髋疼痛症状明显减轻,关节功能不同程度改善,股骨头X线平片及/或CT随访1-6个月（平均4.5个月）示FicatⅠ、Ⅱ期患者介入治疗后股骨头修复明显。结论：股骨头供血动脉内的介入治疗是一种疗效肯定、安全的方法。     

介入治疗股骨头缺血性坏死疗效分析（附38例报告）

目的评价介入治疗股骨头缺血性坏死（ANFH）的疗效。方法对38例ANFH的患者采用介入插管的方法,先常规行股骨头的供血动脉旋股内、外动脉和闭孔动脉造影,观察血液供应情况后,每支血管分别灌注溶栓并给予扩血管药物及改善微循环药物,术后再次造影,观察对比股骨头供血改变情况,并于1～20个月摄X线平片或＼和CT及MRI随访以评估疗效。结果术后血管造影显示所有患者股骨头区血管增多,股骨头染色增强。随访1～20个月（平均10．5个月）影像检查示股骨头缺血性坏死Ⅱ期以内的患者介入治疗后股骨头得以修复。临床症状明显改善。结论介入治疗ANFH是一种安全有效的治疗方法。     

股骨头缺血坏死的介入疗效观察

目的:评价介入治疗股骨头缺血坏死的疗效.方法:对58例股骨头缺血坏死患者采用介入插管的方法,超选择进入到股骨头营养血管旋股内、外侧动脉和闭孔动脉,造影观察其血液供应情况后,注入溶栓药物、扩血管药物及改善微循环药物,术后再次造影观察对比股骨头供血改变情况,并于12～36个月摄X线、CT片与术前X线、CT片进行比较,对比观察股骨头骨质改变情况.结果:治疗前后血管造影对照显示治疗后血管增多,股骨头染色增强,12～36个月后X线、CT片显示骨密度增高者占96.56%,临床症状明显改善.结论:通过介入方法治疗股骨头缺血坏死是一种安全有效的治疗方法,能在临床上广泛应用.     

股骨头无菌性坏死的介入治疗

目的探讨股骨头无菌坏死介入治疗的临床价值。方法经临床、X线、CT或MRI证实的30例患者，采用Seledinger穿刺技术经股动脉插管超选择至旋股内、外动脉，注入溶栓、扩血管及骨营养药物。结果经临床6～48个月的随访，26例患者治疗后股骨头区域血管增加，血管造影改善率86．6％，28例疼痛缓解或消失，临床症状改善率93．3％。结论改善或重建股骨头的血液循环是治疗股骨头坏死的关键，介入导向靶血管溶栓治疗股骨头缺血性坏死治疗有明显疗效，具有积极的临床意义。     

股骨头缺血性坏死的介入治疗68例临床观察

目的探讨血管内介入治疗股骨头缺血性坏死的临床疗效和患者选择。方法对68例股骨头缺血性坏死患者采用Seldinger技术经股动脉穿刺,将导管超选入旋股内、外动脉和闭孔动脉内灌注5～6种扩血管、溶栓药物,利用X线、CT、MRI和血管造影对比治疗前后变化和临床症状的改变。结果本组68例患者临床症状明显好转,X线和CT显示部分病例有新骨形成,56例患者血管造影显示股骨头区血管增多、增粗,血管网增密。结论血管内介入治疗股骨头缺血性坏死是种创伤小、疗效高、见效快的新技术。     

介入法治疗股骨头缺血坏死20例

目的:探讨介入法治疗股骨头缺血性坏死(avascular necrosis of femur head,ANFH)的临床应用价值。方法:20例ANFH患者,全部病例均经X线、CT及MRI检查证实。经股动脉行Seldinger法穿刺插管,先行血管造影,再将导管超选择至病变股骨头供血动脉,灌注一定量溶栓及扩血管药物行介入治疗,术后血管造影观察病变股骨头供血情况,1周后所有病例行第2次介入治疗,方法同前,并随访临床疗效。结果:术后血管造影示股骨头供血动脉均不同程度增粗、增多,血供较术前丰富;随访发现患者关节疼痛缓解率达95%。结论:介入法是治疗ANFH的有效手段,具有重要的临床应用价值。     

股骨头无菌性坏死的X线平片和CT诊断及比较

探讨X线平片与CT平扫对股骨头无菌性坏死的诊断及分期,并结合35例患者X线平片及CT平扫进行图像分析对照。结果显示:CT平扫对股骨头无菌性坏死的诊断优于X线平片。     

股骨头缺血性坏死的介入治疗68例临床观察

目的 探讨血管内介入治疗股骨头缺血性坏死的临床疗效和患者选择.方法对68例股骨头缺血性坏死患者采用se1dinger技术经股动脉穿刺,将导管超选选入股内、外动脉和闭孔动脉内灌注5～6种扩张血管、溶栓药物,利用X线、CT、MRI和血管造影对比剂治疗前后变化和临床症状的改变.结果本组68例患者临床症状明显好转,X线和CT显示部分病例有新骨形成,56例患者血管造影显示股骨头区血管增多、增粗,血管网增密.结论血管内介入治疗股骨头缺血性坏死是种创伤小、疗效高见效快的新技术.     

介入治疗股骨头缺血性坏死的临床研究

目的 探讨通过血管内给药治疗股骨头缺血性坏死的临床效果及本方法的可行性。方法 对35例股骨头无菌坏死的患者，分别进行了经股动脉插管，将血管扩张药、溶栓药及抗凝药、及改善微循环药物直接注入缺血坏死股骨头的供血动脉：旋股内动脉，旋股外动脉及闭孔动脉，治疗后口服维生素D及钙片。结果 经治疗的35例46髋患者中疼痛消失或缓解占9r7．82％，X线、CT复查均有不同程度的骨质修复，新骨形成。血管造影显示股骨头供血动脉增粗，细小动脉侧支循环增多。结论 经股动脉血管内介入治疗股骨头缺血性坏死对改善局部血液循环，缓解临床症状，恢复髋关节功能，促进新骨形成及死骨修复起了重要作用，是一种安全有效的治疗方法。     

介入治疗股骨头无菌性坏死的疗效观察

目的 探讨介入治疗股骨头无菌性坏死的疗效.方法 采用经外周动脉穿刺插管的方法,经血管造影明确供血血管的位置,经导管向其内灌注扩张血管药、舒筋活血中药和溶栓药物,以改善股骨头供血状况;并追踪随访1-3年,根据杨白明评定标准从疼痛、功能、X片3个方面进行判定,观察其临床症状的改善.结果 I-III期股骨头无菌性坏死病人的临床症状减轻,甚至消失;痊愈50%、显效25%、有效15%,(P<0.05).结论 通过对介入治疗股骨头无菌性坏死的疗效观察,发现其介入治疗I-III期股骨头无菌性坏死的疗效显著.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.