高效液相色谱法测定连花清瘟胶囊中苦杏仁苷的含量

目的探讨连花清瘟胶囊中苦杏仁苷的高效液相色谱测定方法,为连花清瘟胶囊的质量控制提供参考。方法采用高效液相色谱法,色谱柱采用Diamonsil-C18色谱柱(4.6mm×250mm,5.0μm),流动相为:乙腈:0.1%磷酸溶液=5:95,检测波长为210nm,柱温为35℃,流速为1.0m L/min。结果 (1)连花清瘟胶囊中苦杏仁苷的线性关系方程为:A=0.0893×10~2C+0.0321×10~2,苦杏仁苷在35.17~215.85μg/m L(r=0.9998)范围内线性关系良好;(2)苦杏仁苷的平均加样回收率为99.76%,99.75%,99.83%;(3)检测限为0.3517μg/m L。结论高效液相色谱法用于对连花清瘟胶囊中有效成分苦杏仁苷含量进行测定,方法操作简单,对胶囊测定方法的重复性好,准确度高,为连花清瘟胶囊的质量控制提供了科学的参考数据。     

高效液相色谱法测定注射用辛芍中野黄芩苷含量

目的: 建立注射用辛芍(冻干粉针)中野黄芩苷的含量测定方法.方法: 采用反相高效液相色谱法,色谱柱:Diamonsil C18柱(4.6 mm×250 mm,5 μm);流动相:甲醇-0.1%磷酸溶液(50∶50);流速:1.0 ml/min;检测波长:335 nm;柱温:40 ℃;进样量10 μl.结果: 野黄芩苷的线性范围为0.030～0.11 mg/L(r =0.999 8),平均回收率为100.2%,RSD为2.3%(n=9).结论: 反相高效液相色谱法测定注射用辛芍,方法准确、简便快速,重复性好.     

HPLC-ELSD法测定苦杏仁中苦杏仁苷含量的研究

目的建立高效液相色谱法-蒸发光散射检测器(HPLC-ELSD)测定苦杏仁中苦杏仁苷含量的的分析方法。方法采用Hypersil-ODS(4.0mm×250mm,5μm)色谱柱;流动相为甲醇-水(30∶70),流速0.5ml/min;柱温25℃;利用ELSD检测器检测:漂移管温度98℃,载气流速3.2L/min。结果苦杏仁苷进样量在1.0￣15.1μg范围内,标准曲线呈良好的线性关系,r=0.9999,苦杏仁苷的平均回收率为99.0%,RSD为2.9%(n=5)。结论本方法简便快速,结果准确可靠,为苦杏仁苷的含量测定提供了一个新方法,可用于苦杏仁药材及含苦杏仁复方制剂的质量控制。     

养心草中槲皮苷的含量测定

目的建立养心草中槲皮苷含量的测定方法。方法用反相高效液相色谱法,色谱柱:AQ-C18(4.6 mm×250 mm,5μm);流动相:甲醇-乙腈-0.1%磷酸溶液梯度洗脱;检测波长:254nm;流速:0.8 ml/min;柱温:30℃。结果槲皮苷在8.16～816μg/mL有良好的线性关系。结论反相高效液相色谱法操作简便,检测灵敏,有良好的重复性和稳定性,可测定养心草中槲皮苷的含量。     

反相高效液相色谱法测定小儿百部止咳糖浆中的苦杏仁苷

目的 建立小儿百部止咳糖浆中苦杏仁苷的测定方法;方法 采用反相高效液相色谱法,色谱柱为Agilent Hypersil ODS, 流动相为甲醇-0.1%磷酸溶液(19∶81),检测波长210 nm。结果 苦杏仁苷在0.081 6～1.020 μg呈良好的线性关系,平均回收率为98.63%,RSD＝1.15%。结论 本方法简便、准确,重现性好。可作为小儿百部止咳糖浆的质量控制标准。     

HPLC法测定库拉索芦荟中芦荟苷的含量

目的: 建立用反相高效液相色谱测定库拉索芦荟中芦荟苷含量的方法.方法: 采用高效液相色谱法,以ZORBAX-C18(4.6 mm×150 mm,5 μm)为色谱柱,流动相为甲醇-1 g/L水-磷酸缓冲液(45∶55∶0.5),检测波长为356 nm,流速1.0 mL/min,柱温25℃.结果: 芦荟苷在0.596～11.9 mg范围内与峰面积呈良好线性关系,r=0.9996(P<0.05),芦荟苷平均加样回收率为97.8%,RSD为1.02%(n＝6).结论: 试验结果表明,本方法可靠性高,准确性和重现性好,适用于库拉索芦荟中芦荟苷的含量测定.     

反相高效液相色谱法测定通窍鼻炎颗粒中欧前胡素和黄芪甲苷的含量

目的建立通窍鼻炎颗粒中欧前胡素和黄芪甲苷的反相高效液相色谱法含量测定方法。方法使用高效液相色谱法进行测定,色谱柱:Kromasil C18柱(5μm,10cm×4.6mm),柱温:25℃,检测波长:欧前胡素260nm,黄芪甲苷210nm,流动相:欧前胡素甲醇-水(55:45),黄芪甲苷乙腈-水(30:70),流速:1.0m L/min。结果本文所建立的分析方法获得较高的理论塔板数。该分析方法准确灵敏,欧前胡素在4.45-44.5μg/m L、黄芪甲在1.24-12.4μg/m L的浓度范围内线性关系良好,回收率和精密度良好。结论使用反相高效液相色谱法测定通窍鼻炎颗粒中欧前胡素和黄芪甲苷的含量其检测方法灵敏,结果准确,重现性好,适用于通窍鼻炎颗粒的质量控制。     

亳菊中木犀草素和木犀草苷含量的反相高效液相色谱法测定

目的:建立毫菊中木犀草素和木犀草苷含量的反相高效液相色谱测定方法.方法:采用反相高效液相色谱法,色谱柱为Scienhome C18(4.6 mm×200 mm,5μm),检测波长350 nm,柱温30℃,流速l mL/min.结果:木犀草苷含量在0.004 8 ～0.038 4 μg内线性良好,Y=10 000 00...     

HPLC法测定柴香疏肝胶囊中芍药苷的含量

目的 采用高效液相色谱法测定柴香疏肝胶囊中芍药苷的含量。方法 采用高效液相色谱法,色谱柱为InertSustain C18柱(4.6mm×150 mm,5 μm),以乙腈-0.1%磷酸溶液(14∶86)为流动相,流速为1.0 mL/min,检测波长为230nm。结果 芍药苷的线性范围为0.1172～2.344 μg(r=0.9999),芍药苷平均加样回收率为99.05%,RSD为2.08%。结论 高效液相色谱法可以用于柴香疏肝胶囊中芍药苷的含量测定。     

枇杷仁中苦杏仁苷的HPLC分析

为建立枇杷仁中苦杏仁苷含量的分析方法,采用高效液相法对苦杏仁苷进行定量分析,色谱柱为Hypersil ODS(4.6mm×250 mm,10 μm),流动相为磷酸盐缓冲液(pH 5.0)-甲醇(8:2),流速为1 ml/min,检测波长为210 nm.苦杏仁苷在10～250μg/ml与峰面积呈良好线性关系,回归方程为Y=17120X 145.99(r=0.9974),平均回收率为99.7%,RSD为1.01%.本方法分离效果好,重现性高,操作简单.可用于枇杷仁中苦杏仁苷的含量测定.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.