5-烯丙基-7-二氟亚甲基白杨素对人肺癌A549细胞裸鼠移植瘤生长的影响

目的 研究5-烯丙基-7-二氟亚甲基白杨素(ADFMChR)对人肺癌A549细胞裸鼠移植瘤生长的影响.方法 建立人肺癌A549细胞裸鼠移植瘤模型,测定荷人肺癌裸鼠移植瘤的大小和重量,应用免疫组化SP法检测移植瘤组织中PCNA、VEGF、CD31的表达.结果 ADFMChR对肺癌移植瘤生长有显著抑制作用(P<0.01),5.0、10.0、20.0 mg/(kg·bw)的ADFMChR对移植瘤的瘤重抑制率分别为42.98%,82.31%和89.91%.免疫组化检测结果表明:ADFMChR具有抑制肺腺癌裸鼠移植瘤细胞PCNA、VEGF及CD31蛋白表达作用.结论 ADFMChR抑制肺腺癌裸鼠移植瘤的生长作用与其抑制移植瘤细胞PCNA、VEGF以及CD31的蛋白表达相关.     

DADS抑制裸鼠肝癌HePG2细胞移植瘤的实验研究

目的：初步探讨二烯丙基二硫化物(DADS)对肝癌细胞的抑制效应及其相关机制。方法：以HepG2细胞为研究对象,进行裸鼠成瘤实验。观察DADS对裸鼠成瘤的影响并绘制生长曲线。应用免疫组织化学方法和Western 印迹技术检测细胞增殖和凋亡相关蛋白的表达,应用TUNEL检测细胞凋亡情况。结果：DADS可抑制裸鼠移植瘤的生长。TUNEL实验结果提示DADS可促进HepG2细胞凋亡。DADS也可促进促凋亡蛋白caspase-3表达并抑制抑凋亡蛋白bcl-2表达和PCNA的表达。结论：DADS可抑制肝癌HepG2细胞在裸鼠体内的生长,与促进细胞凋亡和抑制细胞增殖有关。     

聚焦超声对人宫颈癌裸鼠皮下移植瘤生物学特性的影响

目的:观察聚焦超声对人宫颈癌裸鼠皮下移植瘤治疗作用及对治疗靶区边缘肿瘤组织生物学特性的影响.方法:32例人宫颈癌裸鼠皮下移植瘤模型,不同剂量聚焦超声辐照后,不同时间观察荷瘤鼠的一般生命体征、瘤体生长情况.取辐照靶区及靶区边缘肿瘤组织,观察组织病理学及超微结构变化.原位末端标记法检测瘤细胞凋亡,免疫组化检测Bax、Bcl-2、PCNA及MMP-9的表达.结果:聚焦超声可明显抑制人宫颈癌裸鼠皮下移植瘤的生长,随辐照剂量增加其抑制作用亦增强.病理组织学观察可见各治疗组靶区变性、坏死.核结构破坏.靶区边缘肿瘤细胞肿胀、排列疏松,电镜示线粒体肿胀,胞质空化泡形成.核结构变化不显著.TUNEL检测靶区边缘瘤组织凋亡细胞数目增多,免疫组化检测Bax表达上调.Bcl-2表达下调,PCNA及MMP-9蛋白表达减弱.结论:聚焦超声可明显破坏人宫颈癌裸鼠皮下移植瘤细胞并抑制肿瘤生长,对辐照靶区边缘肿瘤细胞有促进凋亡作用.该过程可能与聚焦超声热效应激活线粒体介导的细胞凋亡信号通路有关.     

蛞蝓有效提取物抑制宫颈癌HeLa细胞裸鼠移植瘤生长

目的 观察蛞蝓有效提取物(EL-3)对宫颈癌HeLa细胞裸鼠移植瘤生长的影响。方法制备宫颈癌HeLa细胞裸鼠移植瘤模型,检查EL-3对瘤体积、瘤重及VEGF蛋白表达的影响,评估EL-3在体内治疗宫颈癌的有效性。结果EL-3(10、30和100 mg/kg)实验组的瘤体积显著低于生理盐水组(P<0.05),同时,其移植瘤的瘤重抑制率分别为42.0%、67.0%和83.0%,提示EL-3在体内抗宫颈癌作用较强,且呈剂量依赖性;采用免疫组化检测证实EL-3下调宫颈癌裸鼠移植瘤细胞VEGF蛋白表达。结论EL-3具有抑制宫颈癌HeLa细胞裸鼠移植瘤生长的作用,其机理可能与抑制肿瘤血管生成有关。     

6,8-二-三氟甲基-7-乙酰氧基白杨素抑制人宫颈癌裸鼠移植瘤生长

目的:研究6,8-二-三氟甲基-7-乙酰氧基白杨素(dFMAChR)的抗宫颈癌作用。方法:体外培养人宫颈癌(HeLa)细胞,采用MTT比色法测定不同浓度dFMAChR作用72 h对HeLa细胞增殖相对抑制率;建立人宫颈癌裸鼠移植瘤模型,评价dFMAChR在体人宫颈癌治疗的有效性。结果:MTT法测定结果显示,dFMAChR抑制体外培养HeLa细胞生长,呈浓度依赖性。人宫颈癌裸鼠移植瘤模型治疗实验结果表明,dFMAChR对人宫颈癌裸鼠移植瘤的生长具有抑制作用,呈剂量和时间依赖性。结论:dFMAChR能抑制人宫颈癌裸鼠移植瘤生长。     

二烯丙基二硫在RORα抑制人胃癌细胞上皮间质转化中的作用

目的探讨二烯丙基二硫（DADS）是否上调维甲酸相关孤核受体α（RORα）抑制人胃癌细胞株MGC803 细胞上皮- 间质转化（EMT）。方法相差显微镜观察MGC803 细胞形态的改变。逆转录PCR（RTPCR）、蛋白免疫印迹法（Western blot）、免疫荧光与免疫组织化学检测EMT 的相关分子表达。裸鼠实验检测DADS 与沉默RORα对移植瘤生长的影响。结果相差显微镜显示,沉默RORα细胞较MGC803 细胞大小与形状差异更明显。DADS作用后,细胞大小较一致,呈圆形或椭圆形,梭形细胞减少,异型性下降。RT-PCR与Western blot显示,DADS 处理后较处理前各组RORα mRNA 与蛋白表达上调（p <0.05）。DADS 作用对照组与空载体组较沉默组效果更为明显（p <0.05）。RORα沉默较对照组和空载体组细胞锌指转录因子（Snail）和波形蛋白（Vimentin）mRNA与蛋白表达上调,而E- 钙黏蛋白（E-cadherin）mRNA 与蛋白下调（p <0.05）。DADS处理后,各组Snail蛋白下调、Vimentin mRNA与蛋白下调和E-cadherin mRNA与蛋白上调（p <0.05）。免疫荧光显示,沉默组细胞Snail与Vimentin阳性表达较对照组增强,而E-cadherin阳性表达减弱。DADS作用后,结果相反。裸鼠移植瘤实验显示,RORα沉默组移植瘤较对照组生长加快和体重增加（p <0.05）,增殖细胞核抗原（Ki-67）、Snail、CD34及Vimentin 阳性表达较对照组增加,而E-cadherin阳性降低。DADS 处理各组移植瘤生长与体积减慢与减小,Ki-67、Snail、CD34与Vimentin阳性表达较对照组减弱,而E-cadherin阳性表达增强。结论DADS 通过上调RORα 可体内外抑制人胃癌MGC803 细胞EMT。     

hTERT启动子驱动的TRAIL联合放化疗对HeLa裸鼠移植瘤生长抑制的作用

目的:探讨hTERT启动子驱动的TRAIL联合放化疗对宫颈癌HeLa裸鼠皮下移植瘤生长抑制的作用. 方法:将含有hTERT启动子的TRAIL基因载体转染宫颈癌细胞HeLa,检测稳定转染细胞中GFP/TRAIL的表达;实验裸鼠分为基因组,化疗组,放疗组,综合组(基因 放化疗),对照组,每组5只,未转染HeLa组为对照组,不作任何处理;将稳定转染基因载体及未转染的HeLa细胞种植于裸鼠背部皮下,联合放化疗,来观察移植瘤成瘤情况,移植瘤的体积、质量以及质量抑制率的变化. 结果:转染hTERT-TRAIL细胞中GFP/TRAIL(绿色荧光蛋白)表达率高于对照组,有统计学意义 (P＜0.01);各组裸鼠在接种细胞第5～7日全部长出肿瘤小结节,结节出现的时间差异无统计学意义(P＞0.05);转染hTERT -TRAIL细胞与对照组HeLa细胞裸鼠的移植瘤质量相比差异有统计学意义(P＜0.05); hTERT -TRAIL细胞在裸鼠体内所形成的移植瘤生长较慢, 4 wk后肿瘤质量抑制率为43.08%,联合放化疗后肿瘤质量抑制率为61.63%,有统计学意义(P＜0.01). 结论:hTERT启动子驱动的TRAIL对人宫颈癌裸鼠移植瘤有明显的抑制作用,联合放化疗能抑制裸鼠移植瘤的生长. 基因治疗联合放化疗等多途径综合治疗为以后的肿瘤研究和临床治疗提供思路.     

消痰散结方对人胃癌MKN-45裸鼠移植瘤生长及细胞增殖相关蛋白表达的影响

目的:观察消痰散结方对裸鼠胃腺癌体内生长的抑制作用及其对胃癌组织中增殖细胞核抗原(proliferatingcell nuclear antigen,PCNA)和表皮生长因子受体(epidermal growth factor receptor,EGFR)表达的影响,探讨消痰散结方抑制胃癌细胞增殖的作用机制。方法:建立人胃腺癌MKN-45裸鼠原位种植模型,将45只模型鼠随机分为荷瘤对照组、中药组及化疗组,接种后次日分别给予生理盐水、中药灌胃及5-氟尿嘧啶(fluorouracil,5-FU)腹腔注射,6周实验结束后,观察移植瘤生长情况,免疫组化抗生物素蛋白链菌素-过氧化物酶(streptavidin peroxidase,SP)连接法检测胃癌组织PCNA及EGFR的阳性表达率,同时观察各组瘤组织的重量及病理学变化。结果:与对照组相比,消痰散结方可明显抑制裸鼠MKN-45胃腺癌移植瘤生长,下调裸鼠胃癌组织中PCNA和EGFR表达(P<0.05)。结论:消痰散结方可通过抑制胃癌细胞核中PCNA表达,干扰DNA合成并影响细胞表面生长因子受体表达,降低细胞增殖活性,抑制移植瘤生长。     

DADS对人结肠癌SW480细胞系增殖的影响

目的观察二烯丙基二硫(DADS)对人结肠癌SW 480细胞增殖的抑制作用。方法实验分DADS处理组和未处理组。MTT法、集落形成实验检测细胞增殖活性,光镜和电镜观察细胞形态学变化,流式细胞术检测周期分布,免疫细胞化学检测增殖细胞核(PCNA)表达改变。结果MTT和集落形成实验显示DADS作用SW 480细胞后,细胞生长抑制率呈浓度、时间依赖性增加,而集落形成率显著降低;组织学显示处理组细胞异型性降低,核质比下降,细胞器丰富;流式细胞仪分析显示DADS明显将细胞周期阻滞于G2/M期,且呈剂量依赖关系;免疫细胞化学显示处理组较未处理组细胞PCNA蛋白表达明显减弱(P<0.05)。结论DADS可体外抑制人结肠癌SW 480细胞增殖,使癌细胞阻滞于G2/M期,可能与下调PCNA蛋白的表达有关。     

抑制NF-κB的表达对人癌裸鼠移植瘤放疗敏感性的影响

目的探讨抑制NF-κB的表达对人癌裸鼠移植瘤放疗敏感性的影响。方法裸鼠皮下接种人宫颈癌HeLa细胞，建立人宫颈癌裸鼠模型。将成瘤的40只裸鼠分为实验组，瘤周注射特异性抑制剂吡咯二硫氨基甲酸脂（PDTC）50mg／kg；对照组，不加PDTC，两组均给予直线加速器照射，剂量分别为0、2、4、6Gy。应用免疫组化方法检测放疗及用药前后人癌裸鼠移植瘤组织NF—κBp65的表达，流式细胞仪检测细胞凋亡百分率，光镜下观察细胞凋亡情况。结果射线诱导的NF—κB表达的增加可以被PDTC抑制；抑制NF—κB的表达使细胞凋亡指数增加。结论放疗诱导的人癌裸鼠移植瘤细胞胞浆内NF-κB的高表达可以通过抑制剂PDTC得到抑制；抑制NF-κB的表达增加了人癌裸鼠移植瘤细胞的放疗敏感性。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.