共查询到19条相似文献,搜索用时 187 毫秒
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目的:了解谷胱甘肽过氧化物酶(GPx)在牙周病变过程中的变化及其临床意义。方法:选择23例成人牙周炎(AP)患者的44颗患牙,记录临床指标GI、PD、AL,并测定治疗前后GCF-GPx水平。结果:GCF-GPx与PD、AL负相关(P〈0.05,P,0.005),与GI无明显相关性。牙周治疗后临床指标均明显下降(P〈0.001),GCF-GPx水平明显上升(P〈0.001),但GCF-GPX水平的上 相似文献
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牙周炎患者龈沟液和龈组织PGE2水平相关性的研究 总被引:8,自引:1,他引:8
为探讨龈沟液(GCF)PGE2含量与龈组织含量的关系,用放射免疫法测定了15名成人牙周炎患者20个部位GCF和相应部位牙龈组织PGE2含量.结果表明PGE2含量:GCF中为69.25±17.22pg/μl,相应牙龈组织为227.47±35.22pg/mg湿重组织,GCFPGE2含量与相应龈组织PGE2含量呈明显的正相关(r=0.9196,P<0.05).提示GCF中PGE2含量可反映出龈组织PGE2水平,为PGE2在牙周病发病机制、病程进展、疗效判定等研究中提供了一敏感、无创伤、可反复取样、简单易行的可靠方法. 相似文献
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牙周病患者治疗前后龈沟液中碱性磷酸酶水平的变化 总被引:11,自引:1,他引:11
选择牙周健康者(H)及边缘性龈炎(MG)、慢性成人牙周炎(CAP),快速进展型牙周炎(RPP)患者共35人,204颗牙齿,测定其龈沟液(GCF)中碱性磷酸酶(ALP)的水平。发现牙周组织有破坏的患牙(CAP和RPP组),其龈沟液(GCF)-碱性磷酸酶(ALP)水平高于牙周无破坏者(H和MG组)GCF-ALP水平与牙周临床指标呈高度正相关,牙周治疗后临床指标及GCF-ALP水平均明显下降。提示GCF 相似文献
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三种牙周炎患者龈沟液中IL—8的检测 总被引:6,自引:1,他引:6
目的:探讨IL-8与不同种类牙周炎的关系,比较三种牙周炎,即青少年牙周炎(juvenileperi-odontitis,JP),快速进展型牙周炎(rapidprogressiveperiodontitis,RPP)和成人牙周炎(adultperiodontitis,AP)龈沟液(gingivalcrevicularfluid,GCF)中IL-8浓度和检出率。方法:采集JP、RPP和AP患者GCF,用ELISA法对GCF中IL-8进行检测。结果:AP患者GCF中IL-8检出率为67.85%,明显高于JP组、RPP组的检出率(分别为34.48%、25%);GCF中IL-8浓度无明显差别。结论:JP、RPP患牙GCF中IL-8检出率低可能是造成此种牙周炎局部中性粒细胞趋化异常的原因之一。 相似文献
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牙本质无机物在牙本质粘接过程中的作用 总被引:1,自引:0,他引:1
目的探讨牙本质无机物在牙本质粘接过程中的作用。方法采用几种不同的牙本质处理方法,测试分析了牙本质与4META/MMATBB树脂之间的抗张粘接强度(tensilebondingstrength,TBS)。结果选用10%柠檬酸和3%三氯化铁组成的酸盐体系预处理液(acidsaltpretreatingsolutionsystem,APSS)去除牙本质污染层,再用次氯酸钠溶解牙本质胶原。暴露牙本质无机物时,牙本质的抗张粘接强度为593MPa,明显高于用蒸馏水冲洗的对照组(371MPa)(P<005)。结论说明牙本质无机物在牙本质粘接过程中确实具有一定的作用 相似文献
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采用RIA对11例健康牙龈组织(H)、11名龈炎(G)患者的17个牙、26名成人牙周炎(AP)患者36个牙和14名青少年牙周炎(JP)患者的20个牙的牙龈组织同时测定PGE_2、TXB_2、6-K-PGF_(1α)的含量。结果以PGE_2最多,TXB_2次之,6-K-PGF_(1α)最少;3种前列腺素(PGs)均随病变严重程度而增加,除G组TXB_2和6-K-PGF_(1α)与H组相差显著(P<0.05)外,其余各病变组与H组比,3种PGs均相差非常显著(P<0.01),AP组和JP组比G组也有非常显著升高(P<0.001),而AP和JP组间相差不显著(P>0.05),表明3种PGs在牙周病发病中具有重要作用。 相似文献
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复发性口疮(RAU)与唾液免疫球蛋白、补体及溶菌酶活性关系的实验研究 总被引:2,自引:0,他引:2
本文对99例复发性口疮患者,分别在初诊治疗前(发作期)和治疗后3月未发作时进行唾液SIgA、IgG、IgM、C3、C4及溶菌酶含量和活性检测,并以45名健康学生唾液相应成份作为正常对照。实验结果表明:复发性口疮患者治疗前唾液SIgA、IgG、IgM、C3、C4及溶菌酶含量和活性,均远远低于正常人唾液相应成份(P<0.01);复发性口疮患者治疗后,其唾液SIgA、IgG、IgM、C3、C4及溶菌酶含量和活性,均较治疗前有显著提高(P<0.01),其中IgG、C4及溶菌酶含量和活性恢复较明显,与正常人唾液相应成份相似(P>0.05)。 相似文献
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TGF—β对人牙乳头间充质细胞增殖和细胞周期的影响 总被引:4,自引:2,他引:2
目的: 探讨TGF- β对人牙乳头间充质细胞增殖和细胞周期的影响。方法:采用MTT法和流式细胞仪观察TGF-β对人牙乳头细胞增殖和细胞周期的影响。结果:50μg/L 组显著刺激人牙乳头细胞增殖(P< 0.05),G1 % 显著降低,S% 明显升高(P< 0 .01),反映细胞增殖活力的增殖指数PrⅠ值(S+ G2 M) 也明显增高( P<0 .01)。结论:TGF-β可能通过促进人牙乳头细胞增殖参与牙胚发育和牙髓损伤修复。 相似文献
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用放射免疫方法测定了16名口腔粘膜鳞癌患者肿瘤组织,癌旁正常口腔粘膜组织,外周血浆及16名正常人外周血浆中cAMP和cGMP含量,并初步探讨了cAMP,cGMP与口腔鳞癌发病的关系。结果显示:与对照组相比,鳞癌患者癌组织中的cAMP水平显著降低)P<0.01),cGMP含量升高(P<0.05),cAMP/CGMP比例下降(P<0.05),而血浆中的cAMP,cGMP含量则无明显改变(P<0.05) 相似文献
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牙周炎患者治疗前后龈沟液中可溶性细胞间粘附分子-1的变化 总被引:7,自引:0,他引:7
了解成人牙周炎患者治疗前后龈沟液中可溶性细胞间粘附分子-1含量的变化。方法:采用常规滤纸条法收集正常成人以及成年人牙周炎患者治疗前后的GCF样本,用ELISA法检测其sICAM-1的含量。结果治疗后GFC中sICAM-1的水平显著下降。 相似文献
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Gonçalves D Correa FO Khalil NM de Faria Oliveira OM Orrico SR 《Journal of clinical periodontology》2008,35(9):799-806
Objective: To evaluate the effect of periodontal therapy on clinical parameters as well as on total salivary peroxidase (TSP) activity and myeloperoxidase (MPO) activity in the gingival crevicular fluid (GCF) of patients with type 2 diabetes mellitus (DM2) and of systemically healthy individuals.
Material and Methods: Twenty DM2 subjects with inadequate metabolic control (test group) and 20 systemically healthy individuals (control group), both groups with chronic periodontitis, were enrolled. Periodontal clinical parameters, namely periodontal probing depth (PD), clinical attachment level (CAL), visible plaque index (VPI), bleeding on probing (BOP), gingival bleeding index (GBI) and presence of suppuration (SUP), as well as TSP activity and GCF MPO activity, were assessed before and 3 months after non-surgical periodontal therapy.
Results: At baseline and 3 months post-treatment, the test group presented a higher percentage of sites with VPI and BOP ( p <0.01). MPO activity in the GCF presented lower values ( p <0.05) for the test group at both baseline and the post-treatment period. The periodontal treatment resulted in a significant improvement of most clinical and enzymatic parameters for both groups ( p <0.05).
Conclusions: In both groups, the periodontal therapy was effective in improving most clinical parameters and in reducing salivary and GCF enzymatic activity. The diabetic individuals presented lower MPO activity in the GCF. 相似文献
Material and Methods: Twenty DM2 subjects with inadequate metabolic control (test group) and 20 systemically healthy individuals (control group), both groups with chronic periodontitis, were enrolled. Periodontal clinical parameters, namely periodontal probing depth (PD), clinical attachment level (CAL), visible plaque index (VPI), bleeding on probing (BOP), gingival bleeding index (GBI) and presence of suppuration (SUP), as well as TSP activity and GCF MPO activity, were assessed before and 3 months after non-surgical periodontal therapy.
Results: At baseline and 3 months post-treatment, the test group presented a higher percentage of sites with VPI and BOP ( p <0.01). MPO activity in the GCF presented lower values ( p <0.05) for the test group at both baseline and the post-treatment period. The periodontal treatment resulted in a significant improvement of most clinical and enzymatic parameters for both groups ( p <0.05).
Conclusions: In both groups, the periodontal therapy was effective in improving most clinical parameters and in reducing salivary and GCF enzymatic activity. The diabetic individuals presented lower MPO activity in the GCF. 相似文献
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Effect of Non‐Surgical Periodontal Treatment on Visfatin Concentrations in Serum and Gingival Crevicular Fluid of Patients With Chronic Periodontitis and Type 2 Diabetes Mellitus
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Background : This study aims to assess visfatin concentrations in serum and gingival crevicular fluid (GCF) and investigate this relationship in patients with type 2 diabetes mellitus (T2DM) and chronic periodontitis (CP) before and after non‐surgical periodontal treatment. Methods: Fifty‐four patients with T2DM and CP were recruited. The patients were randomly divided into two groups: treatment and control. Serum and GCF visfatin concentrations and glycated hemoglobin (HbA1c) levels were measured by enzyme‐linked immunosorbent assay at different time points (at baseline and 3 and 6 months after non‐surgical periodontal treatment). Results: Serum and GCF visfatin concentrations showed no significant differences between the groups at baseline (t test, P >0.05). A significant decline of visfatin in the treatment group was found in serum and GCF 3 months after non‐surgical periodontal treatment (t test, P <0.01). Baseline and 3‐month HbA1c levels were not significantly different, but at 6 months, a statistically significant difference was detected (t test, P >0.05). Conclusions: The data suggest that non‐surgical periodontal treatment is helpful for glucose control, an effect that may be associated with reduced visfatin in patients with T2DM and periodontitis. Furthermore, the data suggest that visfatin may be considered an inflammatory marker for periodontal diseases. 相似文献
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BACKGROUND: Enhanced neutrophil responses play a critical role in the activation of the innate immune system and causation of aggressive periodontitis (AgP). The hypothesis that comprehensive periodontal treatment expedites resolution of amplified leukocyte activity and facilitates the reconstitution of periodontal health was tested. METHODS: Four different gingival crevicular fluid (GCF) markers from 14 patients were characterized prior to and at 3, 6, 12, 24, and 36 months after periodontal therapy. GCF myeloperoxidase (MPO), beta-N-acetyl-hexosaminidase (beta-NAH), and beta-glucuronidase (beta-G) were determined spectrophotometrically, and cathepsin D (CD) by liquid scintillation counting using [14C] hemoglobin as substrate. The primary outcome was long-term stability of periodontal health. RESULTS: In untreated AgP, GCF markers were significantly amplified (MPO: 1.9-fold; beta-NAH: 1.3-fold; beta-G: 1.7-fold; CD: 4.7-fold). Following periodontal therapy, the leukocyte activity was significantly dampened (0.3- to 0.5-fold), and paralleled with a sustained improvement of periodontal health (P < 0.05). Thereafter and at 3 years, GCF leukocyte responses remained on a physiologic low level compatible to normal immune function. CONCLUSIONS: Comprehensive treatment of AP induces a downregulation of amplified crevicular neutrophil activity. The release of the innate immune system from exacerbating damage elicits a successful reconstitution of long-term periodontal health with no setbacks seen after 3 years. 相似文献
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Exaggerated neutrophil responses are a critical component in the pathogenesis of periodontal disease. We investigated whether leukocyte activity in aggressive periodontitis (AP) is increased compared with that in chronic periodontitis (CP) by gingival crevicular fluid (GCF) analysis of myeloperoxidase (MPO), beta-N-acetyl-hexosaminidase (beta-NAH), cathepsin D (CD), and elastase-alpha-1-proteinase inhibitor complex (alpha-1-EPI) before and 6 months after therapy. Initial AP neutrophil responses were significantly amplified compared with those in CP (MPO, 3.2-fold; beta-NAH, 37.5-fold; CD, 2.2-fold; alpha-1-EPI, 1.4-fold; p < 0.05). Surgical therapy resulted in a significant reduction of GCF markers compared with non-surgical treatment. However, the changes in clinical parameters were not different between AP and CP (P > 0.05). Analysis of the results suggests that the local inflammatory response in AP is characterized by increased release of inflammatory mediators of neutrophil origin into the GCF. Analysis of the data further suggests that surgical therapy is a more predictable method for removal of the pro-inflammatory etiology. 相似文献
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目的 :了解聚维酮碘 (PVP -I)和生理盐水配合基础治疗后龈沟液中GPx的变化和临床意义。方法 :2 3名慢性成年性牙周炎 (AP)患者 ,每一测试者选取不同象限的 1~ 2个患牙 ,总共 44颗 2 2对牙在基础治疗完成后 ,一组用 10 % (PVP -I) ,另一组用生理盐水冲洗 ,记录临床指标GI、PD、AL ,并测定治疗前后龈沟液中GPx水平。结果 :两组治疗前后临床指标均明显下降 (p <0.001) ;GPx水平明显上升 (p <0.001) ;PVP - Ⅰ组和生理盐水组 ,各临床指标和GPx水平无显著差异。结论 :AP患者基础治疗后临床指标和生化指标GPx有明显改善 ,但配合使用PVP - Ⅰ和生理盐水冲洗对GCF -GPx水平无明显改善 ,GCF -PGx可能是反映牙周组织状态的一项有意义的指标。 相似文献
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Layik M Yamalik N Caglayan F Kilinç K Etikan I Eratalay K 《Journal of periodontology》2000,71(4):618-624
BACKGROUND: Beta-glucuronidase (betaG) is one of the enzymes involved in the destruction of non-collagenous components of the extracellular matrix. It is also considered an indicator or predictor of periodontal disease activity. The present study was conducted to determine the presence and the levels of betaG activity in gingival tissue and gingival crevicular fluid (GCF) in periodontal disease and health status. The validity of 2 expressions of data, total betaG activity versus betaG concentration, and the correlations between clinical periodontal status and betaG profile was also evaluated. METHODS: betaG activities in gingival tissues and GCF samples from 57 individuals, divided into 3 equal groups of adult periodontitis (AP), early-onset periodontitis (EOP), and periodontally healthy subjects were spectrophotometrically examined. RESULTS: Both patient groups had higher betaG levels in both gingiva and GCF than controls. Significant differences were observed among all groups when total GCF betaG activities were examined (P <0.05). However, the difference between AP and controls was not significant when concentration values were compared (P >0.05). The highest GCF betaG activity, with both expressions, was detected in EOP group. No absolute correlations between clinical parameters and betaG activity were observed, except for random correlations in the patient groups with mean total betaG activities. Also GCF/gingiva betaG levels and the 2 expressions did not show absolute correlations. CONCLUSIONS: The findings of the present study confirm the relationship between betaG activity and periodontal diseases. The differences in data concerning GCF total betaG activity and betaG concentration may suggest that they are not matching measures. Data presentation seems to be an important factor in GCF/enzyme profile studies. 相似文献
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吸烟对牙周基础治疗前后龈沟液量和弹性蛋白酶水平的影响 总被引:4,自引:0,他引:4
目的 评价吸烟是否影响牙周炎基础治疗前、后龈沟液 (gingivalcrevicularfluid ,GCF)量和龈沟液中弹性蛋白酶 (elastase ,EA)的水平。方法 将 37例男性慢性牙周炎患者分为吸烟组 (2 2例 ,12 2个牙位点 ,每日吸烟≥ 2 0支 )和非吸烟组 (15例 ,90个牙位点 )。牙周炎基础治疗前、后用滤纸条法收集GCF ,用Periotron 6 0 0 0龈沟液测量仪测定GCF量。对吸烟组 92个位点和非吸烟组 6 0个位点GCF样本 ,用底物分解法检测EA水平。结果 治疗前吸烟组GCF量 (139 2± 33 4 )U和EA水平(0 6 34± 0 5 87)明显低于非吸烟组 [GCF量 :(15 5 4± 39 7)U ,EA水平 :0 835± 0 5 72 ],P <0 0 1。治疗后 ,两组GCF量和EA水平均显著降低 (P <0 0 0 1)。但吸烟组 91个位点 (74 6 % )GCF和 70个位点(76 1% )的EA水平治疗后有改善 ;而非吸烟组高达 88个位点 (97 8% )GCF和 5 6个位点 (93 3% )的EA水平有改善 (P <0 0 1)。结论 治疗前探诊深度相同的情况下 ,吸烟组GCF量和EA水平均低于非吸烟组 ,治疗后吸烟组的GCF和EA的减少程度不如非吸烟组明显。 相似文献
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Henrique Aparecido Bueno da Silva Vanessa Tubero Euzebio Alves Luis Carlos Spolidório João Batista César Neto Rosangela Santos Eichler Maria Helena Catelli de Carvalho Marinella Holzhausen 《Journal of periodontology》2014,85(12):1763-1769
Background: Protease activated receptor‐1 (PAR1) activation by thrombin may play a role in repair and homeostasis of periodontal tissues. The main objective of this study is to investigate PAR1 expression in patients with periodontitis, before and after non‐surgical periodontal treatment, and to associate its expression with the presence of inflammatory biomarkers and PAR2 expression. Methods: Gingival crevicular fluid (GCF) samples and clinical parameters, including probing depth, clinical attachment level, bleeding on probing, and gingival and plaque indices, were collected from periodontally healthy individuals and patients with moderate chronic periodontitis (CP) before and 6 weeks after periodontal non‐surgical treatment. PAR1 and PAR2 messenger RNA (mRNA) at the GCF were evaluated by quantitative polymerase chain reaction (qPCR). Flow cytometry analysis identified the GCF PAR1‐expressing cells. GCF inflammatory biomarkers were also determined. Results: Clinical parameters were significantly improved after therapy (P <0.01). The qPCR analysis showed that, before therapy, PAR1 mRNA levels in CP were similar to controls. Periodontal treatment led to increased PAR1 expression in CP (P <0.05). PAR1 expression was inversely correlated to PAR2 expression and with interleukins 6 and 8, tumor necrosis factor‐α, interferon‐γ, and matrix metalloproteinase‐2 levels. Conclusions: Periodontal treatment results in PAR1 overexpression in the GCF, and PAR1 expression is associated with decreased expression of inflammatory biomarkers and inversely correlated to PAR2 expression in the GCF. Therefore, the data suggest the importance of PAR1 mediating the known anabolic actions of thrombin in the periodontium. 相似文献