夫西地酸乳膏治疗儿童细菌性皮肤病疗效分析

目的：观察夫西地酸乳膏治疗儿童细菌性皮肤病的疗效和安全性。方法：对34例儿童细菌性皮肤病采用夫西地酸乳膏局部外用治疗，并与34例莫匹罗星软膏组作对照，均每天2次，疗程7天。结果：夫西地酸乳膏治疗儿童细菌性皮肤病有效率为91．17％，与莫匹罗星软膏的有效率（88．2％）相当，无统计学差异（P〉0．05），两组在治疗中都无明显副作用。结论：夫西地酸乳膏治疗儿童细菌性皮肤病疗效肯定，安全性高。     

夫西地酸乳膏治疗化脓性皮肤病的临床疗效观察

目的评价夫西地酸治疗化脓性皮肤病的效果和安全性。方法对60例化脓性皮肤病采用夫西地酸乳膏局部外用治疗,并与60例莫匹罗星软膏组作对照,均3次/d,疗程7天。结果夫西地酸组与莫匹罗星组有效率分别为83.33%和85.00%,细菌清除率分别为87.1%和88.89%,差异均无显著性(P>0.05)。结论夫西地酸乳膏和莫匹罗星软膏治疗化脓性皮肤病的疗效相当,不良反应发生率低。     

0.1%阿达帕林凝胶和2%夫西地酸乳膏联合外用治疗寻常痤疮疗效观察

目的探讨痤疮的有效治疗方法。方法治疗组30例采用阿达帕林凝胶和夫西地酸乳膏联合治疗,对照组28例采用夫西地酸乳膏治疗,8周后结束观察并总结。结果治疗组有效率86.7%,对照组有效率64.3%,两组比较差异有显著性(P<0.05)。结论阿达帕林凝胶和夫西地酸乳膏联合治疗痤疮疗效满意。     

2%夫西地酸乳膏多中心治疗细菌性皮肤病的疗效和安全性观察

目的评价2%夫西地酸乳膏治疗细菌性皮肤病的临床疗效与安全性。方法多中心、开放性试验;外用2%夫西地酸乳膏,2次/d,疗程为7天。结果共有73例细菌性皮肤病纳入研究,其中41例(56.16%)痊愈,29例(39.73%)显效,3例(4.11%)好转,无效0例;有效率为95.89%;治疗结束时未见不良反应。结论2%夫西地酸乳膏外用治疗细菌性皮肤病时,具有良好的临床疗效和安全性。     

夫西地酸乳膏治疗寻常痤疮临床疗效观察

目的评价2％夫西地酸乳膏治疗寻常痤疮的临床疗效及安全性。方法将100例I～II级寻常痤疮患者随机分为两组，一组50例予2％夫西地酸乳膏2次／d涂患处，另一组50例采用红霉素软膏外用，2次／d，14d为1个疗程，共观察28d。结果2％夫西地酸乳膏治疗寻常痤疮治愈率46％，有效率86％，不良反应症状轻微且短暂。结论2％夫西地酸乳膏治疗寻常痤疮疗效肯定且较为安全。     

夫西地酸乳膏联合丙酸氟替卡松乳膏治疗湿疹52例临床观察

目的观察夫西地酸乳膏联合丙酸氟替卡松乳膏治疗湿疹的临床疗效及安全性。方法通过随机数字表法将入选的102例湿疹患者随机分为两组,治疗组52例,用夫西地酸乳膏和丙酸氟替卡松乳膏交替外涂于皮疹处,间隔时间约为1h,早、晚各一次。对照组50例,单用丙酸氟替卡松乳膏外搽,疗程14d。结果治疗组治疗第7天和第14天时有效率分别为50.00%为80.76%;对照组分别为46.00%和62.00%。仅治疗第14天时差异有统计学意义(P0.05)。治疗过程中均未出现严重的不良反应,未经特殊处理均于1~5d消失。结论夫西地酸乳膏联合丙酸氟替卡松乳膏治疗湿疹效果较好,且无明显不良反应。     

夫西地酸乳膏治疗儿童脓疱疮疗效观察

目的观察夫西地酸乳膏治疗儿童脓疱疮的疗效和安全性。方法将2007年6月～2008年6月就诊的100例脓疱疮患儿分为2组,治疗组50例,外搽2%夫西地酸乳膏3次/d,对照组50例,外搽2%莫匹罗星软膏3次/d,疗程均为7天。结果治疗组有效率为94.00%,对照组为92.00%,两组有效率比较,差异无显著性意义(P>0.05),治疗期间均未出现不良反应。结论2%夫西地酸乳膏治疗儿童脓疱疮有效,安全性高。     

2%夫西地酸乳膏治疗细菌感染性皮肤病临床观察

目的评价2%夫西地酸乳膏治疗细菌感染性皮肤病的疗效和安全性。方法60例细菌感染性皮肤病患者,治疗组30例外擦2%夫西地酸乳膏,2～3次/d;对照组30例外擦2%莫匹罗星软膏,3次/d,疗程7天。结果治疗组有效率86.6%;对照组有效率90.0%,两组疗效差异无显著性(P>0.05)。治疗期间未出现不良反应。结论2%夫西地酸乳膏与莫匹罗星软膏对各种感染性皮肤病均有较好疗效,安全性高,患者依从性好。     

夫西地酸乳膏联合卤米松乳膏治疗湿疹的疗效评价

目的观察夫西地酸乳膏联合卤米松乳膏治疗湿疹的临床疗效和安全性。方法治疗组交替使用夫西地酸乳膏和卤米松乳膏,2次/d,每种药物使用间隔时间超过2h以上。对照组单独使用卤米松乳膏,2次/d,两组疗程均为2周。结果治疗组有效率为89.09%,对照组有效率为68.63%,两组有效率比较差异有统计学意义(P0.05)。结论夫西地酸乳膏联合卤米松乳膏治疗湿疹疗效好,安全性高。     

湿疹伴细菌感染定植的检测和治疗

目的： 分析湿疹皮损处的细菌定植情况并评价地奈德乳膏与夫西地酸乳膏联合外用治疗的疗效。 方法： 84例湿疹患者分两组：A组外用地奈德乳膏（2次/天）和夫西地酸乳膏（2次/天）,B组外用地奈德乳膏（2次/天）,分别于治疗后7天和14天观察疗效,治疗前皮损处取材做细菌培养和药敏。结果： 治疗7天和14天后,A组患者的有效率为71.43%和88.09% 明显高于B组的42.86%和69.05%（P=0.008和P=0.033）。84例湿疹患者中 45例患者皮损处培养鉴定细菌阳性,包括金葡菌32株（38.09%）,表皮葡萄球菌10株（11.9%）,大肠埃希菌2株（2.38%）,溶血性链球菌1株（1.19%）。结论：湿疹皮损易伴发微生物感染,地奈德联合夫西地酸外用治疗伴发细菌感染的湿疹可提高疗效。     

Changes in fungal ultrastructure after short-course ciclopiroxolamine therapy in pityriasis versicolor

The clinical outcome and the effects on morphogenesis and cell ultrastructure induced by a 1% ciclopiroxolamine solution in six patients with proven pityriasis versicolor were studied. Treatment regimens consisting of a once-daily application for 1 day, 2 days (Days 1 and 4) or 3 days (Days 1, 4 and 8). Clinical evaluation, scanning (SEM) and transmission electron microscopy (TEM) were performed on skin scrapings before treatment, and at 3, 7, 15 and 21 days after the start of therapy. SEM techniques have shown severe changes in the surface ultrastructure of yeasts and hyphae of Pityrosporum spp. (Malassezia furfur) 15 days after the start of therapy. TEM techniques showed extensive internal disruption, mainly severe necrosis of the cytoplasm, 3 and 7 days after the start of treatment. However, KOH direct microscopy showed apparently normal morphology of Pityrosporum spp. (Malassezia furfur) at all assessment points. The final clinical cure in all the patients was achieved 21 days after the start of therapy.     

Efficacy of povidone-iodine vaginal suppositories in the treatment of bacterial vaginosis

A prospective, randomized clinical trial was performed to study the efficacy of povidone iodine (Betadine) suppositories for the treatment of bacterial vaginosis (BV) in comparison to capsules containing lactobacilli (D?derlein Med). Seventy patients with BV included in the study were randomly assigned to be either treated with povidone iodine suppositories or lactobacilli. Patients were treated once a day for 5 days. Initial examinations took place on the first day of the study with follow-up examinations on days 8 and 15. The examinations included clinical parameters, patient evaluation, secretion screens, and quantitative and qualitative microbiological tests of vaginal flora. Both treatment groups showed improvement of clinical parameters, condition of secretions and subjective state of health. At day 15 there was a trend towards a better efficacy of the treatment with povidone iodine but this was not significant. However, patients with acute BV treated with povidone iodine had significantly better scores after 15 days. Both treatments were well tolerated. The microbiological examinations showed an increase of the mean number of lactobacilli in the vagina on day 8 after initiation of treatment with lactobacilli, but a decrease on day 15. Contrary to that the lactobacilli counts from patients treated with povidone iodine suppositories decreased after the first week but increased in the second one. Potentially pathogenic germs, e.g. Gardnerella, Bacteroides and Enterobacteria were reduced in a higher extent and with a longer lasting effect after treatment with povidone iodine suppositories than with capsules containing lactobacilli. The results of this study show that native lactobacilli rapidly re-colonize after the antiseptic treatment with povidone iodine. Therefore, there is no need to use lactobacilli in addition.     

Methodologic aspects in cultivating human keratinocytes

The cultivation of human keratinocytes is an appropriate experimental model for a variety of cell biological, pharmacological and biochemical investigations and the production of in vitro epidermis suitable for grafting since Rheinwald and Green (1975) have established this method. A procedure is described to isolate and culture human keratinocytes using different culture conditions. About 1-2 million epidermal cells could be prepared by trypsinization over night at 4 degrees C from 1-2 cm2 skin. Primary cell cultures seeded on mitomycin C treated NIH 3T3 fibroblasts reached subconfluence after 15-20 days when Eagle MEM was used as culture medium and after 10-15 days of culture using a mixture of Dulbecco's MEM and Ham F12 (3:1). In each case the media were supplemented with different growth factors. Subcultures were seeded on NIN 3T3 feeder cells as well as in collagen or fibronectin coated dish. Multilayered epidermal sheets developed with in 12-15 days of culture.     

Investigations on the development and regression of corticosteroid-induced thinning of the skin in various parts of the human body during and after topical application of amcinonide

In 15 subjects 6 different parts of the body were treated with the potent topical corticosteroid (CS) amcinonide once a day for 21 days. The skin thickness of the treated areas was measured sonographically at intervals of 2 days. After completion of the CS application the regression of the CS-induced reduction in skin thickness was measured for a further 14 days. A clear thinning of the skin could be measured after only 4 days and continued up to the 21st day of treatment. This thinning regressed completely in all areas after 10-12 days. The thinning and regression curves were very similar for all body areas. Differences in intensity of thinning were evident. No tachyphylaxis was detected.     

1320nm激光对小鼠皮肤组织病理和生理特性的影响

目的:研究1320 nm激光对小鼠皮肤组织病理和部分皮肤生理指标的影响。方法:用该波长激光对小鼠背部皮肤照射,于照射前、照射后1 h3、d7、d、15 d、30 d取皮肤做病理,同时用MPA皮肤多功能测试系统测定其皮肤回声值、黑素含量、皮肤血红蛋白含量、经皮失水量、表皮含水量和皮脂含量。结果:激光照射后1 h,真皮纤维组织排列致密。照射后3 d,真皮炎症细胞和成纤维细胞增多。照射后15 d真皮层增厚、胶原增加。激光照射后短期内对于皮肤弹性、皮肤血红蛋白含量、经皮失水量、皮脂含量、表皮含水量,均有一定程度暂时性影响,对皮肤黑素含量无影响。结论:1320 nm激光可引起小鼠成纤维细胞增生和胶原蛋白合成增加,改善皮肤弹性,但在短期内对皮肤生理状态可产生一定的负面影响。     

表现为复发性水疱的肘管综合征一例

患者,女,15岁。左前臂持续青紫,反复水疱2年余。结合临床症状和神经电生理检查,诊断为肘管综合征。给予甲钴胺、维生素B1治疗25天后大部分糜烂面痊愈。     

Measurement of transepidermal water loss using an evaporimeter to follow the restitution of the barrier layer of human epidermis after stripping the stratum corneum

The rate of evaporation after stripping a test area with adhesive tape was followed in 10 women over a period of 15 days. The much increased transepidermal water loss after stripping diminished rapidly on the first 3 days. It then decreased more slowly, approaching but not fully reaching the normal rate by the end of test period. This can be taken to reflect the repair process of the damaged barrier layer. The instrument used makes possible instantaneous reading of the rate of evaporation, and is highly suited for assessing the function of the barrier layer.     

秋水仙碱联合小剂量强的松治疗结节性痒疹的疗效观察

目的：研究秋水仙碱联合小剂量强的松治疗结节性痒疹的疗效。方法：结节性痒疹患分成两组，每组30例；治疗组患服用秋水仙碱片0．5mg隔tid，强的松片15mg qa，艾洛松霜外用；对照组患服用强的松片15mg qa，艾洛松霜外用；两组疗程均为4周；每周随访记录每例患皮损变化情况、瘙痒减轻情况及不良反应，疗程结束后记录疗效。结果：治疗组总有效率为80％，而对照组为53．3％(P＜0．05)；治疗组和对照组的瘙痒减轻平均时间为6天和9天(P＜0．01)；两组结节开始消退平均时间为14天和20天(P＜0．01)。结论：秋水仙碱联合小剂量强的松治疗结节性痒疹具有起效快、有效率高、使用安全等特点，是一种较理想的治疗结节性痒疹的方法。     

Erythromycin stearate in treating chlamydial infection of the cervix.

A total of 157 women attending departments of genitourinary medicine were treated for chlamydial infection of the cervix with erythromycin stearate 500 mg twice a day. Chlamydiae were eradicated from the cervix in 64/80 women treated for seven days and in 51/77 women treated for 14 days. In 12 of those treated for seven days and 15 of those treated for 14 days, reinfection was the probable cause of reisolation after treatment. The possibility of latent infection with Chlamydia trachomatis could not be excluded in five women, but was not more likely to occur with the shorter treatment course. Erythromycin stearate 500 mg twice daily for seven days appears to be an effective regimen for the treatment of uncomplicated chlamydial infection of the cervix.     

Erythromycin stearate in treating chlamydial infection of the cervix

A total of 157 women attending departments of genitourinary medicine were treated for chlamydial infection of the cervix with erythromycin stearate 500 mg twice a day. Chlamydiae were eradicated from the cervix in 64/80 women treated for seven days and in 51/77 women treated for 14 days. In 12 of those treated for seven days and 15 of those treated for 14 days, reinfection was the probable cause of reisolation after treatment. The possibility of latent infection with Chlamydia trachomatis could not be excluded in five women, but was not more likely to occur with the shorter treatment course. Erythromycin stearate 500 mg twice daily for seven days appears to be an effective regimen for the treatment of uncomplicated chlamydial infection of the cervix.