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1.
Objective
To observe the effects of moxibustion at different times on prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and arginine vasopressin (AVP), in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention.Methods
Forty-three female Wistar rats were randomly divided into a blank control group (n=7), a model group (n=9), a pre-moxibustion group (n=9), an immediate-moxibustion group (n=9) and a pre-moxibustion plus immediatemoxibustion group (n=9). Rat models of primary dysmenorrhea due to cold-dampness retention were established using (0±1) °C ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11th day. Rats in the 3 intervention groups received moxibustion to Shenque (CV 8) and Guanyuan (CV 4), 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11th day during modeling, for 4 continuous days. The level of PGF2α in the rat uterine tissues was measured by enzyme-linked immunosorbent assay (ELISA), and the levels of PGE2 and AVP in rat uterine tissues were measured by radioimmunoassay.Results
Compared with the blank control group, the levels of PGF2α and AVP, the PGF2α/PGE2 ratio in the model group were significantly increased (P<0.01), and the PGE2 level was significantly decreased (P<0.01) in the rat uterine tissues in the model group. Compared with the model group, the writhing latency was significantly prolonged, the writhing number and the total writhing score were all decreased in the pre-moxibustion group, the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group (all P<0.01); the levels of PGF2α and AVP, and the PGF2α/PGE2 ratio were all significantly decreased (P<0.05, P<0.01), and the PGE2 level was significantly increased (P<0.01) in the rat uterine tissues of the 3 treatment groups. Compared with the pre-moxibustion group, the writhing number and the total writhing score were all decreased in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group (all P<0.01), the writhing latency was significantly prolonged in the pre-moxibustion plus immediate-moxibustion group(P<0.01); the levels of PGF2α and PGF2α/PGE2 ratio were significantly decreased (P<0.05, P<0.01), and the PGE2 level was significantly increased (P<0.01) in rat uterine tissues in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group. Compared with the immediate-moxibustion group, the writhing latency was significantly prolonged and the writhing number was decreased (all P<0.05), and the total writhing score was decreased (P<0.01) in the pre-moxibustion plus immediatemoxibustion group; the PGF2α level and the PGF2α/PGE2 ratio were significantly decreased (P<0.01), and the PGE2 level was significantly increased (P<0.01) in rat uterine tissues in the pre-moxibustion plus immediate-moxibustion group.Conclusion
Moxibustion at different times all can produce obvious analgesic effects on dysmenorrhea due to cold-dampness retention in rats, and pre-moxibustion plus immediate-moxibustion ranks the top. The mechanism of this analgesic effect may be via the regulation of abnormal PGF2α, PGE2 and AVP levels, to effectively inhibit the spastic contraction of uterine smooth muscle in dysmenorrhea rat, thereby improving the ischemia and hypoxia in uterus.2.
Qiong LIU Wei ZHU Mailan LIU Xia LIU Jianan CAO Shuning HU Xiaorong CHANG Xianghong JING 《世界针灸杂志》2018,28(4):257-262
Objective
To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells (MCs) and 5-hydroxytryptamine (5-HT) in local acupoint area of Guānyuan (关元 CV 4) and serum 5-HT in rats.Methods
Thirty-nine male C57BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5?min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30?min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method (5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay (ELISA) (8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared.Results
① After acupuncture stimulus or thermal stimulus on CV 4 of C57BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group (the acupuncture stimulus group 12.40?±?2.07 vs. the blank group 3.00?±?5.96; thermal stimulus group 26.20?±?10.85 vs. the blank group 12.40?±?2.07, both P?<?0.05), and MC aggregation and degranulation were observed (the acupuncture stimulus group 17.80?±?4.55 vs. the blank group 8.00?±?3.16; the thermal stimulus group 24.00?±?9.05 vs. the blank group 8.00?±?3.16, P?<?0.05, P?<?0.01). ② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels, and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group (the blank group 3.00?±?1.28 vs. the acupuncture stimulus group 10.02?±?3.21; the blank group 3.00?±?1.28 vs. the thermal stimulus group 14.00?±?3.94, both P?<?0.01). ③ Compared with blank group, both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum (the blank group 0.72?±?0.2372 vs. acupuncture stimulus group 0.43?±?0.21; the blank group 0.72?±?0.24 vs. thermal stimulus group 0.32?±?0.18, both P?<?0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group (P?<?0.05).Conclusion
Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation. 相似文献3.
Xin-hua LI Xiao-xue SUN Yu-lei LIANG Fei GAO Xiao-yi DU Xiao-hong ZHOU Yang CHEN Xue-na LI Li-hong SUN Xiao-shun MA 《世界针灸杂志》2017,27(2):29-34
Objective
To observe the effect of instant moxibustion on the levels of prostaglandin E2 (PGE2), prostaglandin F2α (PGE2α) and arginine vasopressin (AVP) in the uterine tissues of dismenorrhea rats with cold-damp congealing and stagnation type and to explore its possible mechanism.Methods
Female Wistar rats were randomly divided into blank group, model group, herble medicine group, pre-moxibustion group and instant moxibustion group, with 9 rats in each group. Cold-damp congealing and stagnation type primary dismenorrhea models were established by adopting (0±1)°C ice water-extraction method combined with estradiol benzoate injection method. After modeling on the 8th day, in herble medicine group, Tongjingbao granules was given to the rats by intragastric administration. In pre-moxibustion group, mild moxibustion was carried out at “Shénquè” (Results
Compared with the model group, the latent period of rats in herbal medicine group, pre-moxibustion group and instant moxibustion group obviously prolonged, the number of times of torsion reduced, and the total score of torsion decreased (P<0.01); compared with herbal medicine group, the latent period of rats in instant moxibustion group obviously prolonged, and the total score of torsion decreased (P<0.05 or P<0.01); compared with pre-moxibustion group, the number of times of torsion of rats in instant moxibustion group reduced, and the total score of torsion decreased (P<0.01). Compared with blank group, the levels of PGE2α and AVP and the ratio of PGE2α and PGE2 in the uterine tissues of rats in model group significantly increased (P<0.01), and the PGE2 level significantly reduced (P<0.01); compared with model group, the PGE2α level and the ratio of PGE2α and PGE2 in the uterine tissues of rats in herble medicine group, pre-moxibustion group and instant moxibustion group obviously reduced (P<0.05 or P<0.01), the PGE2 level obviously increased (P<0.01), and the AVP level in the uterine tissues of rats in pre-moxibustion group and instant moxibustion group obviously reduced (P<0.05 or P<0.01); compared with herbal medicine group, the levels of PGE2α and AVP and the ratio of PGE2α and PGE2 in the uterine tissues of rats in instant moxibustion group significantly reduced (P<0.05 or P<0.01); compared with pre-moxibustion group, the PGE2α level and the ratio of PGE2α and PGE2 in the uterine tissues of rats in instant moxibustion group obviously reduced (P<0.05), and the PGE2 level obviously increased (P<0.01).Conclusion
Both pre-moxibustion and instant moxibustion can obviously inhibit spasmodic uterine smooth muscle contraction of rats with dismenorrhea, regulate imbalanced levels of PGE2α and PGE2, reduce the AVP level, so as to improve the uterine hypoxia-ischemia, and play a role in alleviating pain. The efficacy of instant moxibustion was superior to that of pre-moxibustion. 相似文献4.
Objective
To observe the effect of thermosensitive moxibustion on anxiety and depression in the patients of insomnia differentiated as liver qi stagnation.Methods
From January 2015 to January 2017, 60 patients of insomnia differentiated as liver qi stagnation were collected in Shijiazhuang Municipal Chinese Medicine Hospital. According to the random number table, the patients were randomized into a moxibustion group (30 cases) and an estazolam group (30 cases). In the moxibustion group, the thermosensitive moxibustion was adopted alternatively to the bilateral yuan-source points of the liver and gallbladder meridians. In the estazolam group, estazolam, 1?mg was prescribed for oral administration before sleep every day. After 15-day treatments, the sleep quality, the severity of anxiety and depression and the therapeutic effects were observed before and after treatment in the two groups.Results
Before treatment, the differences were not significant in the scores of Pittsburgh sleep quality index (PSQI), the self-rating anxiety scale (SAS) and the self-rating depression scale (SDS) between the two groups (all P?>?0.05). After treatment, the scores of PSQI, SAS and SDS were all reduced remarkably as compared with those before treatment in the two groups (all P?<?0.05). PSQI score was (6.72?±?2.311) points in the moxibustion group and was (5.37?±?2.621) points in the estazolam group. SAS score was (31.76?±?6.511) points in the moxibustion group and was (39.62?±?4.371) points in the estazolam group. SDS score was (35.98?±?5.161) points in the moxibustion group and was (46.38?±?4.971) points in the estazolam group. After treatment, the scores of PSQI, SAS and SDS in the moxibustion group were reduced more remarkably as compared with the estazolam group, indicating the significant differences (all P?<?0.05). After treatment, the scores of sleep efficacy and TCM symptoms were (72.65?±?14.36) points and (69.36?±?4.28) points respectively in the moxibustion group, better than the estazolam group, indicating the significant differences (all P?<?0.05).Conclusion
The thermosensitive moxibustion at the yuan-source points of the liver and gallbladder meridians significantly improves the sleep quality, relieves the symptoms of anxiety and depression and enhances the therapeutic effects in the patients of insomnia differentiated as liver qi stagnation. Hence, this therapy deserves to be recommended in clinical practice. 相似文献5.
Objective
To observe the clinical efficacy of acupuncture–moxibustion therapy of "banking up original qi and consolidating the foundation" for prevention and treatment of seasonal allergic rhinitis.Methods
Sixty included patients were divided into two groups according to the random number table method with 30 patients in each group. In acupuncture group, warming-needle moxibustion was carried out at Zúsānl? (足三里ST 36), and cone moxibustion on Fùz? (附子Radix Aconiti Lateralis Praeparata) cake was conducted at Guānyuán (关元CV 4), Qìh?i (气海CV 6) and Dàzhuī (大椎GV 14). The treatment was conducted in May or June (2 months before the onset of rhinitis in this area). The treatment was carried out for once every other day and three times per week. Treatment for 24 times was considered as 1 course of treatment. No intervention treatment was conducted in control group. The score of rhinitis symptom, the visual analogue scale (VAS) score changes of total nasal symptom score (TNSS) and total non-nasal symptom score (TNNSS) of patients in 2015 and in 2016 were observed, and the clinical efficacy of the two groups was compared.Results
(1) The score of rhinitis symptom, the VAS score changes of TNSS and TNNSS in acupuncture group in 2016 declined when compared with the results in 2015, which were 4.57?±?0.82?vs 9.40?±?1.89, 13.37?±?4.01?vs 30.80?±?4.77 and 15.93?±?6.30?vs 46.13?±?9.79 (P?<?0.001), the results in acupuncture group after treatment were lower than that in control group at same time, which were 4.57?±?0.82?vs 7.53?±?2.06, 13.37?±?4.01?vs 27.17?±?6.84,15.93?±?6.30?vs 35.53?±?11.69(all P?<?0.001). According to comparison, there was no obvious difference in the results in control group at the two time points, which were 8.60?±?2.39?vs 7.53?±?2.06, 29.63?±?6.30?vs 27.17?±?6.84 and 40.90?±?12.31?vs 35.53?±?11.69 (all P?>?0.05). (2) The total effective rate was 96.7% (29/30) in acupuncture group and 10% (3/30) in control group, and the difference was statistically significant (P?<?0.001).Conclusion
Acupuncture–moxibustion therapy of "banking up original qi and consolidating the foundation" was effective on prevention and treatment of seasonal allergic rhinitis. 相似文献6.
Objective
To observe the effect of warming yang and supplementing kidney moxibustion therapy on the behavioristics and the expression levels of hippocampal IL-1β, TNF-α and NF-κB related genes and proteins of vascular dementia (VD) rats, and to explore the mechanism of warming yang and supplementing kidney moxibustion therapy in inhibiting VD inflammatory response. MethodsSeventy-eight SD rats, except 10 rats as sham operation group (group A), were established into VD models by applying the ischemia reperfusion method in bilateral common carotid arteries. 30 models were successful and were randomly divided into model group (group B), moxibustion group (group C) and western medication group (group D), with 10 rats in each group. After modeling, suspended moxibustion was conducted at “Dàzhuī (大椎GV 14)”, “Mìngmén (命门GV 4)” and “Guānyuán (关元CV 4)” of the rats in group C for 15?min/time. The intervention was performed for once a day, and intervention for 4 consecutive weeks was needed. Intragastric administration with nimodipine (2?mg?kg?1?d?1) was carried out in the rats in group D for 4 consecutive weeks. Morris water maze test was adopted for behavioral test in the rats in each group. HE staining was conducted in order to observe the pathological changes. RT-qPCR method and Western blot method were used for detecting the expression levels of hippocampal IL-1β, TNF-α and NF-κB related genes and proteins.Results
After modeling, the Morris water maze escape latency of rats in operation groups was obviously longer than that in group A (P?<?0.05). According to HE staining results, the arrangement of neurons in group B was incompact, the cell membranes were undefined, the cytoplasm was heterogeneous, karyopyknosis was found, the amount of necrosis increased, and the number of inflammatory cells significantly increased. Compared with group B, the number of inflammatory cells significantly reduced in group C and group D, and the amount of neuron necrosis also reduced. After intervention, compared with group B, the expression levels of TNF-α, p-IκB and NF-κBp65 mRNA in the hippocampal of the rats in group C and group D significantly decreased (all P?<?0.05), compared with group B, the expression levels of IL-1β, TNF-α and NF-κBp65 mRNA in the hippocampal of rats in group C and group D also significantly decreased (all P?<?0.05).Conclusion
Warming yang and supplementing kidney moxibustion therapy may inhibit the NF-κB signaling pathway and reduce the inflammatory response through lowering the expression levels of IL-1β, TNF-α and NF-κB mRNA in the brain, thus achieving the purpose of treating VD. 相似文献7.
Jiang-yun WU Yi-ni SUN Qing-chen ZHOU Lei WANG Zhi-hong WEN Xue-si HOU Shu-han QU Yi-fan JIA Ji-ping ZHAO 《世界针灸杂志》2018,28(3):204-208
Objective
To explore the tenderness response at the acupoints on the medial crus in the patients of pelvic inflammatory disease.Methods
A total of 30 patients of pelvic inflammation and 30 healthy people were included. WAGNER FDX body mechanics algometer was used to determine the score of the visual analogue scale (VAS) and tenderness threshold value at Yīnlíngquán (阴陵泉 SP 9), Sānyīnjiāo (三阴交SP 6) and Lígōu (蠡沟 LR 5). The changes in the tenderness on the body surface at the relevant acupoints were compared and analyzed in the patients of pelvic inflammation.Results
The occurrence rate of tenderness at LR 5 at the pelvic inflammation group was higher significantly than the health group (86% Vs 42%, P<0.01). In SP 9, SP 6 and LR 5,the VAS scores at the acupoints in the pelvic inflammation group were higher significantly than the health group (test 1:55.00?±?15.12 vs 27.25?±?10.31, 47.07?±?18.38 vs 29.75?±?14.30, 47.16?±?19.4 vs 20.16?±?10.76; test 2:53.40?±?17.23 vs 33.42?±?13.07, 45.95?±?15.74 vs 29.15?±?11.97, 42.50?±?21.67 vs 21.05?±?11.97; test 3: 48.50?±?14.97 vs 40.08?±?13.20, 38.24?±?15.29 vs 29.29?±?12.37, 37.93?±?19.17 vs 23.09?±?12.26) , P?<?0.01, P?<?0.05 .In SP 9, SP 6 and LR 5,the tenderness threshold values at the acupoints in the pelvic inflammation group were higher significantly than the health group (test 1:0.86?±?0.95 vs 0.53?±?0.39, 0.86?±?0.95 vs 0.53?±?0.39, 0.85?±?0.77 vs 0.47?±?0.47; test 2:0.88?±?0.81 vs 0.44?±?0.32, 0.98?±?0.83 vs 0. 44?±?0. 32, 0.85?±?0.77 vs 0.47?±?0.47; test 3:0.98?±?0.80 vs 0.36?±?0.26, 1.11?±?0.92 vs 0. 36?±?0. 26, 0.85?±?0.77 vs 0.47?±?0.47), P?<?0.01, P?<?0.05 (P?<?0.05).Conclusion
The VAS scores and tenderness threshold values at SP 9, SP 6 and LR 5 in the patients of pelvic inflammation are higher significantly than those in the healthy people. The occurrence rate of tenderness at LR 5 is higher significantly as compared with the healthy people. 相似文献8.
Objective
To evaluate and compare electroacupunctures (EA) with different parameters and moxibustion at different temperatures influencing the activation of mast cells (MC) in Tianshu (ST 25) regions of visceral hyperalgesia model rats.Methods
Rats (except for model group) respectively accepted 1 mA or 3 mA EA or moxibustion at 43 °C or 4 °C to stimulate Tianshu (ST 25) points after randomization of the fifty visceral hyperalgesia model rats, and then were compared with that in model and normal groups. Number, degranulation numbers, degranulation rates in Tianshu (ST 25) regions MC of rats in each group were observed using toluidine blue staining. Abdominal withdrawl reflex (AWR) score was used to evaluate the rat visceral hyperalgesia reactions.Results
Compared with the normal group and the model group, MC numbers (P<0.05, P<0.01, P<0.01, P<0.01), degranulation numbers and degranulation rates (P<0.01, P<0.01, P<0.05, P<0.01) of Tianshu (ST 25) MC in regions tissues in 43 °C and 4 °C moxibustion groups, and 1 mA and 3 mA EA groups all increased significantly. Compared with the model group, AWR scores were significantly lower in 43 °C and 4 °C moxibustion groups, and 1 mA and 3 mA EA groups under the stimulation of 20 mmHg, 40 mmHg, 0 mmHg or 80 mmHg colorectal distension (CRD) (P<0.05 in 1 mA and 3 mA EA groups under the stimulation of 20 mmHg, P<0.01 in the other groups). AWR scores in 43 °C and 4 °C oxibustion groups under the stimulation of 20 mmHg, 40 mmHg, 0 mmHg or 80 mmHg CRD were not significantly different from those in the normal group (all P>0.05); AWR scores in 1 mA EA group under the stimulation of 0 mmHg or 80 mmHg were significantly higher than that in the normal group (P<0.01); AWR score in 3 mA EA group under the stimulation of 0 mmHg was significantly higher than that in the normal group (P<0.01), and AWR scores in 3 mA EA group under the stimulation of 20 mmHg or 80 mmHg were also higher than that in the normal group (P<0.05). AWR scores were higher in 1 mA EA group under the stimulation of 40 mmHg or 80 mmHg than that in 4 °C moxibustion group (P<0.05); AWR score was higher in 3 mA EA group under the stimulation of 40 mmHg than that in 4 °C moxibustion group (P<0.05).Conclusion
There are differences among EA of different parameters and moxibustion of different temperatures in activating on Tianshu (ST 25) regions MC of visceral hyperalgesia model rats, as well as in improving the visceral hyperalgesia reaction. The effect of 4 °C moxibustion is the most significant.9.
Li-jia PAN XU Jing Xin ZHANG Xuan-ping ZHANG Rui JIA Yu-zhu DU Hai-jiao XING Chun-sheng JIA 《世界针灸杂志》2017,27(2):35-42
Objective
To explore the effects of different acupuncture-moxibustion methods on the plasma substance P (SP) content, and the expression of colonic SP mRNA of the rats with functional constipation.Methods
Sixty healthy male SD rats were divided into six groups according to the random number table, including blank control group (group B, n=8), model group (group M, n=11), drug control group (group D, n=8), filiform needle group (group F, n=11), electroacupuncture group (group E, n=11) and moxibustion group (group MB, n=11). The rats in group B and group M accepted no intervention, gavage with cisapride suspension was conducted in group D, and electroacupuncture, filiform needling and moxibustion therapies were carried out in group E, group F and group MB for 6 days. After treatment, the stool quantity and fecal moisture content during 24 h of the rats were observed in each group, colon tissue structure and the acidic mucus in mucosa layer were observed, plasma SP content was detected and the expression of colonic SP mRNA was detected.Results
Compared with group B, the stool quantity and fecal moisture content during 24 h of the rats in group M decreased significantly (P<0.01); compared with group M, the stool quantity during 24 h of the rats in each group increased (P<0.05 or 0.01), and the fecal moisture content during 24 h of the rats in each group also increased significantly except group MB (P<0.01); compared with group D, the fecal moisture content during 24 h of the rats in group MB decreased (P<0.01); according to the comparison among the groups with different acupuncture-moxibustion methods, there was no difference in the stool quantity during 24 h of the rats in each group (P>0.05), the fecal moisture contents during 24 h of the rats in group F and group E were higher than that of group MB (P<0.01), and there was no difference between group F and group E (P>0.05). HE staining of colon tissue of rats showed that the structure was normal and complete, and there was no difference among each group. PAS staining showed that the acidic mucus level of the rats in group M decreased significantly when compared with group B; compared with group M, the acidic mucus level of the rats in group D, fgroup F, group E and group MB increased in varying degrees. Compared with group B, the plasma SP content of the rats in group M decreased (P<0.05); compared with group M, the plasma SP contents of the rats in group F and group MB increased (P<0.01); the plasma SP contents of the rats in group F, group MB and group E were higher than that of group D (P<0.05 or 0.01); according to the comparison among the groups with different acupuncture-moxibustion methods, the plasma SP content of the rats in group F was higher than that of group MB and electro-acupuncture group (P<0.05), and there was no difference between group MB and group E. Compared with group B, the expression level of colonic SP mRNA of the rats in group M elevated (P<0.05); compared with group M, the expression level of colonic SP mRNA of the rats in the four intervention groups declined (P<0.05); compared with drug group D, there was no difference among group F, group E and group MB (P>0.1); according to the comparison among the groups with different intervention methods, the expression level of colonic SP mRNA of the rats in group MB was lower than that of group F (P<0.05).Conclusion
The different efficacy of different acupuncture-moxibustion methods in treatment of functional constipation may be related with the mechanism of regulation of plasma SP content and the expression level of colonic SP mRNA. 相似文献10.
Objective： To observe the effect of moxibustion on intestinal propulsion rate, content of serum D-xylose, content of adenosine triphosphate （ATP） and activities of adenosine triphosphatases （ATPase） in jejunum tissues of spleen-deficiency rats, and to explore the effect of moxibustion on the production of ATP and activities of membrane proteins, for analyzing the mechanism of moxibustion in reinforcing the spleen-stomach. Methods： Forty healthy Sprague Dawley rats were randomly divided into four groups, namely group A （blank control group）, group B （model control group）, group C （moxibustion group） and group D （herbs group）. The animal model of spleen-deficiency was established by intragastric administration with Da Huang （Radix et Rhizoma Rhei） infusion. The rats in group C received moxibustion at Zusanli （ST 36）, Zhongwan （CV 124 Guanyuan （CV 4）, Pishu （BL 20） and Weishu （BL 21）. The rats in group D received intragastric administration with 5i Jun Zi decoction. The intestinal propulsion was measured by toner method, and the content of D-xylose in serum was detected by phloroglucinol method. Colorimetry method was used to detect the content of ATP and activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in jejunum tissues. Results： Compared with group A, the intestinal propulsion rate, content of D-xylose in serum, content of ATP, activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATP in jejunum tissues decreased significantly in group B （P〈0.05 or P〈O.O1）. Compared with group B, the intestinal propulsion rate, content of D-xylose in serum, content of ATP, activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in jejunum tissues increased significantly in group C and D （P〈0.05 or P〈0.01）, while there were no significant differences between group C and group D. Conclusion： Moxibustion can increase the intestinal propulsion rate, content of D-xylose in serum and ATP in jejunum tissues, as well as the activities of Na~-K＋-ATPase and Ca^2＋-Mg^2＋-ATPase of spleen-deficiency rats, which suggests that moxibustion can enhance the motility and absorption functions of small intestine by promoting the production of ATP in intestinal epithelial cells, increasing the content of membrane proteins, and improving transmembrane transport. 相似文献
11.
Qin QI Lu-yi WU Huan-gan WU Hui-rong LIU Yuan-yuan WANG Ya-nan LIU Cun WANG Ru-de HUANG Xiao-mei WANG 《世界针灸杂志》2017,27(3):40-62
Objective
To explore the regulating effect of moxibustion on the enteric nervous system of rats with Crohn's disease.Methods
Ten SD rats were selected randomly from 40 rats as normal control (group A), and the other 30 rats were established into Crohn's disease rat models by adopting clysis method with TNBS. On the basis of modeling successfully, the model rats were randomly divided into model group (group B), herbs-partitioned moxibustion group (group C) and mild moxibustion group (group D) with 8 rats in each group (4 rats were dead during modeling. After modeling, 2 rats were selected from group A, and 2 rats were selected from models for determination, at last, 8 rats were included in each group). In group C and group D, herbs-partitioned moxibustion or mild moxibustion was applied on “Tiānshū (Results
Compared with group A, the expression levels of proteins of S-100, SP and its receptor NK1R, NPY and its receptors NPY1R and NPY2R in the intestinal tract of rats in model groups increased obviously, and the differences were statistically significant. (Ps-100<0.01, PSP<0.05, PNK1R<0.01, PNPY<0.05, PNPY1Y<0.05, PNPY2R<0.01), after treatment with drug-paste interposed moxibustion and mild moxibustion, the levels reduced significantly (PS-100<0.05, PSP<0.05, PNK1R<0.01, PNPY<0.05, PNPY1R<0.05, PNPY2R<0.01).Conclusion
Moxibustion treatment may regulate the expression levels of proteins of S-100, SP, NK1R, NPY, NPYIR and NPY2R through warm stimulation, alleviate inflammatory response of colonic tissue, and repair impaired colonic mucosa, thus achieving the goal of treating Crohn's disease. 相似文献12.
Objective
To observe the effect of acupuncture at Jǐngjiājǐ (颈夹脊) on the repair and regeneration of cochlear hair cells of guinea pigs with sensorineural deafness.Methods
Sixty healthy guinea pigs were selected, 20 guinea pigs were randomly assigned to the normal control group (group A), and other guinea pigs were randomly divided into model control group (group B) and acupuncture treatment group (group C) after injection with gentamicin sulfate in order to induce deafness. No intervention was given to the guinea pigs in group A and group B, and acupuncture at Jǐngjiājǐ was given to the guinea pigs in group C for 30 days. ABR threshold, DPOAE amplitudes and hair cells counting of guinea pigs in each group were recorded after intervention for 30 days.Results
After intervention for 30 days, ABR threshold in group C was significantly lower than that in group B (38.46?±?7.36?vs 82.94?±?6.47, P<0.01), and the DPOAE amplitudes in group C were obviously higher than that in group B (28.06?±?5.64?vs 25.23?±?5.38, P<0.01). The number of cochlear hair cells in group C increased significantly, over 50% of the hair cells survived, accounting for 66.67% of the observation cases. The number of cochlear hair cells in the 3rd and 4th gyri was close to the normal level, and plenty of proliferous sustentacular cells can be seen. Compared with group B, the number of cochlear outer hair cells in each gyrus in group C significantly increased (36.76?±?1.97?vs 28.59?±?2.24, P<0.01), indicating that acupuncture at Jǐngjiājǐ can promote the repair and regeneration of cochlear hair cells.Conclusion
Acupuncture at Jǐngjiājǐ can promote the repair and regeneration of cochlear hair cells, thus improving the hearing of guinea pigs with deafness. 相似文献13.
Baozhu Zheng Ling HuXiaoge Song Zijian WuRonglin Cai Lu HeCheng Zhang Qing Yu 《Journal of traditional Chinese medicine》2014
Objective
To observe the influence of different moxibustion durations on hypothalamic pro-opiomelanocortin (POMC) and prodynorphin (PDYN) mRNA expressions and plasma β-endorphin (β-EP) content in rheumatoid arthritis (RA) rats, to understand the mechanism of moxibustion analgesia and its dose-effect relationship.Methods
Twelve male Wistar rats were randomly selected from 48 male Wistar rats as a normal control group. The RA model was created by raising rats in a windy (blowing with electric fan), cold (6°C ± 2°C), and wet (80%-90% humidity) environment for 20 days, 12 h each day. This was followed by injection of Freund's complete adjuvant (0.15 mL) into the ankle. Then, rats were randomly divided into a model group, moxibustion group I, and moxibustion group II, with 12 rats in each group. In moxibustion groups I and II, moxibustion was given at Shenshu (BL 23) and Zusanli (ST 36) for 20 and 40 min, respectively, once daily for 15 days. Hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content were determined.Results
Compared with the normal group, the pressure pain threshold decreased, while the hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content increased in the moxibustion groups (P<0.01). Compared with the model group, the pressure pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in the moxibustion groups increased significantly (P<0.01). Compared the moxibustion group I, the pain threshold, hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in moxibustion group II significantly increased (P<0.01).Conclusion
Moxibustion has an analgesic effect and increases hypothalamic POMC and PDYN mRNA expression levels and plasma β-EP content in RA rats. The analgesic effect in moxibustion group II is better than that in moxibustion group I. 相似文献14.
Objective
To explore the therapeutic effects of scalp acupuncture on sleep disorder induced by pre-examination anxiety in the undergraduates.Methods
A total of 60 undergraduates with sleep disorder induced by the final examination anxiety were randomized into three groups, named a control group (20 cases), a traditional acupuncture group (20 cases) and a scalp acupuncture group (20 cases). In the control group, no any treatment was given. In the traditional acupuncture group, acupuncture was given at Sìshéncōng (四神聪EX-HN1), bilateral Shénmén (神门HT 7) and bilateral Sānyīnjiāo (三阴交SP 6). The needles were manipulated with the even-needling technique and retained for 30?min. In the scalp acupuncture group, acupuncture was applied to upper-middle line of occiput (枕上正中线MS 12), middle line of vertex (顶中线MS 5) and middle line of forehead (额中线MS 1). The needles were manipulated with the even-needling technique and retained for 30?min. The treatment was given once a day and 5 treatments made one course. There were 2 days at interval among the courses. The therapeutic effects were analyzed statistically after 4 courses of treatment. Before and after treatment, the Hamilton anxiety scale (HAMA) and the Pittsburgh sleep quality index (PSQI) were adopted to evaluate the conditions of pre-examination anxiety and sleep in the patients.Results
In comparison of PSQI score, compared with those before treatment, the score in the scalp acupuncture group (12.95?±?1.76?vs 15.95?±?1.82) and in the traditional acupuncture group (13.75?±?1.62?vs 15.75?±?1.86) after 1-week of treatment were reduced (both P?<?0.05). Compared with control group, the score in the scalp acupuncture group (12.95?±?1.76 vs15.78?±?2.02) and in the traditional acupuncture group (13.75?±?1.62?vs 15.78?±?2.02) were better (both P?<?0.05). The difference was not significant between the scalp acupuncture group and the traditional acupuncture group (P?>?0.05). In 2 and 4 weeks of treatment, as well as 1 week after treatment termination, compared with the control group at the same time point, the scores in the scalp acupuncture group (in 2-week treatment: 8.95?±?1.88?vs 15.94?±?1.89, in 4-week treatment: 5.95?±?1.05?vs 16.5?±?1.95, 1 week after treatment termination: 4.7?±?0.77?vs 9.78?±?2.10) and the traditional acupuncture group (in 2-week treatment: 11.15?±?1.31?vs 15.94?±?1.89, in 4-week treatment: 8.05?±?0.89?vs 16.5?±?1.95, 1 week after treatment termination: 6.25?±?0.85?vs 9.78?±?2.10) were all lower (all P?<?0.05) separately. The scores in the scalp acupuncture group were superior to the traditional acupuncture group (all P?<?0.05). In comparison of HAMD score, compared with those before treatment, the score in the scalp acupuncture group (22.4?±?5.31?vs 25.2?±?6.18) and in the traditional acupuncture group (22.4?±?5.31?vs 25?±?5.97) after 1-week of treatment were reduced (both P?<?0.05). Compared with control group, the score in the scalp acupuncture group (22.4?±?5.31?vs 23.28?±?5.53) and in the traditional acupuncture group (22.4?±?5.31?vs 23.28?±?5.53) were better (both P?<?0.05). The difference was not significant between the scalp acupuncture group and the traditional acupuncture group (P?>?0.05). In 2 and 4 weeks of treatment, as well as 1 week after treatment termination, compared with the control group at the same time point, the scores in the scalp acupuncture group (in 2-week treatment: 18.5?±?4.56?vs 24.22?±?5.22, in 4-week treatment: 5.8?±?3.52?vs 21.22?±?6.7,1 week after treatment termination: 4.55?±?2.72?vs 11.78?±?9.36) and the traditional acupuncture group (in 2-week treatment: 17.5?±?4.59?vs 24.22?±?5.22, in 4-week treatment: 6.95?±?3.33?vs 21.22?±?6.7, 1 week after treatment termination: 5.8?±?2.76?vs 11.78?±?9.36) were all lower (all P?<?0.05) separately. The scores in the scalp acupuncture group were superior to the traditional acupuncture group (all P?<?0.05). In 4-week treatment and 1 week after treatment termination, compared with control group, the total effective rates in the traditional acupuncture group (in 4-week treatment: 95% vs 11%, 1 week after treatment termination: 100% vs 83.33%, both P?<?0.05) and the scalp acupuncture group (in 4-week treatment: 95% vs 11%, 1 week after treatment termination: 100% vs 83.33%, both P?<?0.05) were better and the difference was not significant between the traditional acupuncture group and the scalp acupuncture group (both P?>?0.05).Conclusion
Both scalp acupuncture and traditional acupuncture therapies are effective on sleep disorder induced by pre-examination anxiety in the undergraduates. The scalp acupuncture therapy achieves the better effects on the instant relief of symptoms. 相似文献15.
ObjectiveTo explore the influence of scarring moxibustion on the changes of serum melatonin (MT) and neuropeptide Y (NPY) expressions in the insomnia model rats.MethodsA total of 40 SD rats were randomly divided into a blank group, a model group, a scarring moxibustion group, an embedding needling group and a mild moxibustion group, 8 rats in each group. The intraperitoneal injection of p-chlorophenylalanine (PCPA) was adopted to establish the insomnia model rats and the corresponding interventions were given, consecutively for 6 days. Separately, before modeling and the first intervention as well as before sample collection, the body weight was measured and the serum was collected in the insomnia model rats. The enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum MT and NPY.ResultsCompared with the blank group, the body weight of rat in the model group was lower (P < 0.01) and the levels of MT and NPY were lower in the model group (both P < 0.05). Compared with the model group, in the scarring moxibustion group, the embedding needling group and the mild moxibustion group, the body weight and the levels of MT and NPY of the insomnia model rats were all higher (all P < 0.05). Compared with the embedding needling group and the mild moxibustion group, the body weight and the levels of MT and NPY the insomnia model rats were all higher in the scarring moxibustion group (all P < 0.05). In comparison between the embedding needling group and the mild moxibustion group, there were no statistical differences in the body weight and the levels of MT and NPY (all P > 0.05).ConclusionThe regulation mechanism of sleep-wake circadian rhythm may be related to the levels of serum MT and NPY in the insomnia model rats. Scarring moxibustion increases the levels of MT and NPY more effectively and displays the effect in the intervention as compared with embedding needling and mild moxibustion in the insomnia model rats. 相似文献
16.
Objective
To explore influence of electroacupuncture (EA) therapy of tonifying the kidney and regulating governor vessel on amyloid beta (Aβ) related degradation enzymes in the hippocampus of a rat model of Alzheimer's disease (AD) induced by Aβ1-42.Methods
Forty Wistar male rats were randomly divided into 4 groups: a normal group, a sham operation group, a model group and an EA group, 10 rats in each one. The rats in normal group were normally fed. The rats in sham operation group were bilaterally injected in the hippocampus with 5 µL of saline and they were normally fed after the injection. The rats in the model group and the EA group were bilaterally injected in the hippocampus with 5 µL of Aβ1-42 on each side. Rats in the EA group received EA of 5?Hz continuous wave at the “B?ihuì (百会 GV20)” and bilateral “Shènshū (肾俞 BL23)” for a duration of 15 min per time every day and continuously for 15 days. After 15 days, the hippocampal expression levels of insulin degrading enzyme (IDE), lipoprotein (LPL), transthyretin (TTR), apolipoprotein E (APoE), α2 macroglobulin (α2M) and Aβ1-42 of the 4 groups were tested by Western blot.Results
Compared with the sham operation group, the expression levels of IDE, LPL, TTR, APoE and α2M in the hippocampus were significantly lower (P?<?0.05, P?<?0.01) and the expression of Aβ1-42 was significantly higher(P?<?0.01) in the model group. Compared with the model group, the expression levels of IDE, LPL, TTR, APoE and α2M in the hippocampus of these rats were significantly lower (P?<?0.05, P?<?0.01), the expression of Aβ1-42 was significantly higher(P?<?0.01) in the EA group.Conclusion
EA therapy of tonifying the kidney and regulating governor vessel can enhance the expression of IDE, LPL, TTR, APoE, and α2M in the hippocampus of AD rats injected by Aβ1-42, and may consequently promote the degradation of aβ1-42 to help improve the pathological manifestations of AD and therefore delay its progression. 相似文献17.
Objective
To observe the effect of herbal cake-partitioned moxibustion on the expressions of mitogen-activated protein kinase (MEK1/2) and extracellular regulatory protein kinase (ERK1/2) in gastric tissues of rats with spleen deficiency syndrome, and to explore the possible mechanisms of herbal cake-partitioned moxibustion in treating spleen deficiency syndrome.Methods
Sixty Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a ranitidine group (group C), and a herbal cake-partitioned moxibustion group (group D) by random digit, 15 rats in each group. Rat models of spleen deficiency syndrome were made by intragastric administration of 4 °C 200% concentrated Da Huang (Radix et Rhizoma Rhei). After successful modeling, the rats in group C were treated with 25 mg/(kg·bw) ranitidine by intragastric adminstration and rats in group D were treated with herbal cake-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12), for 8 d. Excepted for rats in group A, all the other rats were treated with indomethacin at 5 mg/(kg·bw) at 8:00 a.m. on the second day after finishing all the intervention and sacrificed 7 h later to isolate the stomach. Histopathological changes of the gastric tissues were observed under light microscope after hematoxylin-eosin (HE) staining. The protein expressions of MEK1/2 and ERK1/2 in the gastric tissues were detected by immunohistochemistry.Results
After intervention, the gastric mucosal injury in group B was significantly severer than that in group A, with large breakage and ablating; the damage of gastric mucosa was decreased in group C compared with group B; the gastric mucosal surface remained relatively complete, and the status of breakage and ablating was significantly improved. After intervention, compared with group A, the protein expressions of MEK1/2 and ERK1/2 in gastric tissues of the other groups were significantly higher (P<0.01). Compared with group B, the protein expressions of MEK1/2 and ERK1/2 in group C and D were significantly higher (all P<0.01). Compared with group C, the protein expressions of MEK1/2 and ERK1/2 in group D were significantly higher (P<0.01).Conclusion
Herbal cake-partitioned moxibustion promotes the repair of gastric mucosa in rats with spleen deficiency syndrome, via improving protein expressions of MEK1/2 and ERK1/2 in gastric tissues, as well as activating MEK/ERK signaling pathway.18.
ObjectiveTo explore the effect differences between moxibustion and donepezil hydrochloride on the attention network function of patients with mild cognitive impairment (MCI).MethodsA total of 64 patients of MCI were randomly divided into the moxibustion group and donepezil hydrochloride group, 32 cases in each one. On the basis of conventional treatment, the patients in the moxibustion group were given moxibustion, 6 times a week, and the patients in the donepezil hydrochloride group were given donepezil hydrochloride orally, 5 mg / day. The course of treatment was 60 days for both of the groups. Cognitive attention network function and activities of daily living (ADL) score were examined before and after treatment.ResultsThe differences of alerting reaction time (RT), executive control RT, overall mean RT and accuracy of the moxibustion group after treatment were significantly higher than those of the donepezil hydrochloride group [alert: (60.3 ± 3.3) ms vs (48.3 ± 3.7) ms, P < 0.05; executive control: (81.2 ± 3.2) ms vs (91.7 ± 4.2) ms, P < 0.05; total reaction time: (500.4 ± 17.2) ms vs (536.2 ± 20.1) ms, P < 0.05; accuracy: (83.7 ± 4.6)% vs (77.4 ± 4.3)%, P < 0.05]. After treatment, the ADL scores of the both groups were significantly higher than those before treatment [the moxibustion group: (56.47±4.02) points vs (41.53±4.06) points, P < 0.05; the donepezil hydrochloride group: (50.75±4.05) points vs (40.84±3.67) points, P < 0.05], and the ADL score of the moxibustion group was significantly higher than that of the donepezil hydrochloride group [(56.47±4.02) points vs (50.75±4.05) points, P < 0.05].ConclusionCompared with donepezil hydrochloride, moxibustion has a better effect on the cognitive function of MCI patients. 相似文献
19.
Objective
To observe the effect of moxibustion on learning and memory abilities, corticosterone and glucocorticoid receptor (GR) in subacute aging rats.Methods
Twenty four Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group and a moxibustion group, 8 rats in each group. Rats in the model group and the moxibustion group were subcutaneously injected with 25% D-galactose [125 mg/(kg·bw)] for 40 d continuous; rats in the normal group were injected with saline at the same position for 40 d continuous. Rats in the moxibustion group were given mild moxibustion at bilateral Shenshu (BL 23) at the same time of modeling; rats in the normal group and the model group were only identically grabbed without moxibustion for 40 d. The learning and memory abilities of rats were observed using the Morris water maze at the end of the experiment. Abdominal aorta blood and thymus were collected after water maze experiment. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum corticosterone level, and immunohistochemical method was used to detect the expression of thymus GR.Results
Compared with the normal group, rats in the model group showed that a significantly longer escape latency time (P<0.01) on the third and the fourth days; number of times crossing the platform in 70 s significantly reduced (P<0.01); activity times in the fourth quadrant significantly decreased (P<0.05); serum corticosterone levels increased (P<0.01); thymus GR expression decreased (P<0.05). Compared with the model group, rats in the moxibustion group showed that the escape latency times were significantly shorter on the third, the fourth and the fifth days (P<0.01, P<0.05); number of times crossing the platform in 70 s significantly increased (P<0.05); activity times in the fourth quadrant significantly increased (P<0.05); serum corticosterone levels decreased (P<0.05); thymus GR expression increased (P<0.05).Conclusion
Moxibustion could improve the learning and memory abilities of subacute aging rats, down-regulate serum corticosterone levels, and increase thymus GR content.20.