镉对大鼠四种器官乳酸脱氢酶同工酶的影响

目的：观察镉对大鼠器官乳酸脱氢酶（LDH）同工酶的影响;方法：采用聚丙稀酰胺凝胶垂直板电泳法结合光密度扫描法,分析在四种镉中毒浓度（0．2mg／kg、0.4mg／kg、0．8mg／kg和1.6mg／kg）下的SD大鼠心、肝、肾和脑中LDH同工酶的变化。结果：随着镉中毒浓度的升高,心脏LDH各同工酶的活性显著升高,而肾脏LDH各同工酶的活性显著下降,脑中的LDH1和LDH2的活性出现先升高后下降现象。镉对肝脏中LDH各同工酶的影响不显著。结论：镉可能对不同器官中LDH同工酶的H、M亚基的基因表达影响不一。     

镉对大鼠四种器官乳酸脱氢酶同工酶的影响

目的：观察镉对大鼠器官乳酸脱氢酶（ＬＤＨ）同工酶的影响;方法：采用聚丙稀酰胺凝胶垂直板电泳法结合光密度扫描法,分析在四咱镉中毒浓度（０．２ｍｇ／ｋｇ、０．４ｍｇ／ｋｇ、０．８ｍｇ／ｋｇ和１．６ｍｇ／ｋｇ）下的ＳＤ大鼠心、肝、肾和脑中ＬＤＨ同工酶的变化。结果：随着镉中毒浓度的升高,心脏ＬＳＨ各同工酶的活性显著升高,而肾脏ＬＤＨ各同工酶的活性显著下降,脑中的ＬＤＨ１和ＬＤＨ２的活性出现选知高后下降现象     

血清保存时间对乳酸脱氢酶同工酶活性的影响

含M 亚基多的LDH 同工酶既不耐热,又不耐冷,保存易失活.我们观察36例血清标本,在4℃放置1～6天对结果的影响,报告如下.一、材料与方法1.材料取30例正常人和6例肝癌患者静脉     

乳酸脱氢酶及其同工酶在胸腔积液中的临床意义

目的 探讨乳酸脱氢酶（ＬＤＨ）及其同工酶对鉴别诊断胸腔积液中良、恶性疾病及监测其病程预后的临床价值。方法 将２０７例伴有胸水的肺癌、结核性胸膜炎及肺炎、心功能不全、心力衰竭患者分为三组,ＬＤＨ采用酶法检测,其同工酶则以醋纤琼脂糖电泳法进行酶谱分析。分别检测患者胸水和血清ＬＤＨ及其同工酶。结果 结核性胸膜炎患者胸水ＬＤＨ增加显著,为１２９４±２１２．９Ｕ／Ｌ与自身血清相比有显著差异（Ｐ〈０．００１）     

安宫牛黄丸对脑梗死大鼠血清乳酸脱氢酶同工酶的影响

背景：安宫牛黄丸因含有汞、砷成分，其安全性受到关注，有必要进行有效性和安全性评价研究。目的：研究生理、病理状态下安宫牛黄丸对机体作用的差异。设计：以实验动物为研究对象的随机对照实验。单位：一所大学的临床药理研究所。材料：实验于2001-03/04在广州中医药大学临床药理研究所完成，广东省医学实验动物中心提供体质量250～300g SD雄性大鼠24只。方法：SD大鼠随机分成4组：正常组；正常+安宫牛黄丸组；脑梗死模型组(光化学诱导大鼠大脑中动脉闭塞)；脑梗死模型+安宫牛黄丸组。每组6只。给药方法：每天胃饲1次，0．13g／kg，共7d。主要观察指标：血清乳酸脱氢酶同工酶LDH1-5含量。结果：正常+安宫牛黄丸组大鼠血清LDH1-3含量比正常组显著升高(P&;lt;0．01)，其中正常+安宫牛黄丸组LDH1，LDH2，LDH3的值分别为(17．02&;#177;0．46)，(14．70&;#177;0．18)，(15．47&;#177;013)％，正常组则为(11．25&;#177;0．70)，(8．26&;#177;0．90)，(12．86&;#177;0．90)％；模型+安宫牛黄丸组大鼠血清LDH，(15．51&;#177;2．60，％)比模型组(10．93&;#177;2．10，％)显著升高(P&;lt;0．01)，LDH4含量显著降低，其值分别为(22．62&;#177;3．00)％，(28．18&;#177;0．80)％(P&;lt;0．01)。结论：在局灶性脑梗死病理状态下安宫牛黄丸对机体的损伤作用比在正常生理状态下小，提示安宫牛黄丸在生理、病理状态下，对机体的作用方式存在差异。     

心梗和肝病血清乳酸脱氢酶同工酶图型变化的分析

作者测定了68例心梗(AMI)及172例肝脏疾病的血清LDH总活力和LDH同工酶图型并与90名正常人的图型进行了比较分析。68例AMI的图型为LDH_1↑、LDH_2↓、LDH_1/LDH_2比值>1.8;52例肝癌的典型图型倾向于LDH_1↓、LDH_2↓、LDH_3↑、LDH_5↑;54例急性肝炎的典型图型为LDH_1↓、LDH_2↓、LDH_5↑。66例肝硬化的典型图型为LDH_2↓、LDH_5↑。各同工酶仍保持正常的百分率、称为同型性图型。     

安宫牛黄丸对脑梗死大鼠血清乳酸脱氢酶同工酶的影响

背景安宫牛黄丸因含有汞、砷成分,其安全性受到关注,有必要进行有效性和安全性评价研究.目的研究生理、病理状态下安宫牛黄丸对机体作用的差异.设计以实验动物为研究对象的随机对照实验.单位一所大学的临床药理研究所.材料实验于2001-03/04在广州中医药大学临床药理研究所完成,广东省医学实验动物中心提供体质量250～300 g SD雄性大鼠24只.方法SD大鼠随机分成4组正常组;正常+安宫牛黄丸组;脑梗死模型组(光化学诱导大鼠大脑中动脉闭塞);脑梗死模型+安宫牛黄丸组.每组6只.给药方法每天胃饲1次,0.13 g/kg,共7 d.主要观察指标血清乳酸脱氢酶同工酶LDH1～5含量.结果正常+安宫牛黄丸组大鼠血清LDH1～3含量比正常组显著升高(P＜0.01),其中正常+安宫牛黄丸组LDH1,LDH2,LDH3的值分别为(17.02±0.46),(14.70±0.18),(15.47±0.13)%,正常组则为(11.25±0.70),(8.26±0.90),(12.86±0.90)%;模型+安宫牛黄丸组大鼠血清LDH3(15.51±2.60,%)比模型组(10.93±2.10,%)显著升高(P＜0.01),LDH4含量显著降低,其值分别为(22.62±3.00)%,(28.18±0.80)%(P＜0.01).结论在局灶性脑梗死病理状态下安宫牛黄丸对机体的损伤作用比在正常生理状态下小,提示安宫牛黄丸在生理、病理状态下,对机体的作用方式存在差异.     

乳酸脱氢酶同工酶,脂蛋白,尿蛋白同步琼脂糖凝胶电泳技术探讨

本文对脂蛋白、尿蛋白琼脂糖凝胶电泳中的泳动槽缓冲液和琼脂糖凝胶的配制及泳动条件进行了改进,使之与乳酸脱氢酶同工酶测定相同,可同步电泳,然后分别染色求出测定值。此法简便快速,可常规应用。     

乳酸脱氢酶同工酶的临床意义分析

乳酸脱氢酶(LDH)同工酶广泛存在于各组织器官中.测定LDH同工酶活性增高情况,在一定程度上可反映组织器官的病变.     

实验性心肌损伤对大鼠房室束LDH、SDH的影响

目的　观察心肌损伤时大鼠房室束乳酸脱氢酶 (LDH)、琥珀酸脱氢酶 (SDH )的变化 ,研究心肌损伤对心传导组织功能的影响。方法　SD大鼠 2 9只 ,随机分为对照组和不同时间心肌损伤 (0 5、1、3、6、12h)实验组。实验组皮下注射异丙肾上腺素 5mg/kg。NBT法染色显示LDH、SDH。将房室束分为未分叉部和分叉部 ,高倍镜 (× 40 0 )下测量各部组化反应的平均灰度值。结果　对照组和实验组房室束各部的LDH、SDH的染色均比普通心肌弱。心肌损伤时 ,房室束各部LDH的活性短时间 (0 5h)内明显增强 ,而SDH的活性则下降。从房室束的未分叉部到分叉部 ,LDH、SDH的活性减弱。结论　房室束的有氧代谢、无氧代谢均比普通心肌低 ;房室束各部的糖代谢不均衡 ;心肌损伤早期对大鼠房室束的脱氢酶有较明显的影响。     

Elevated level of serum LDH2 and LDH3 in sputum three positive TB patients of Sahariya tribe: a preliminary study

OBJECTIVES: The objective of this investigation was to find out if sputum-positive (AFB test) test, which is performed to assess mycobacterial infection status, is anyway correlated with any of the LDH isoforms. And if so, can it be used, either alone or together with sputum test, as a rapid on-the-spot marker for field diagnosis of tuberculosis. DESIGN AND METHODS: To analyze the relationship between sputum test results and the level of LDH isozyme (isoforms), 157 individuals were randomly selected from a Sahariya tribal population, with a known history of tuberculosis, for sputum and blood collection. The Ziehl-Neelsen's staining of sputum smear was done as per RNTCP (Revised National Tuberculosis Control Programme) protocol. In all the samples, serum LDH level was estimated spectrophotometrically while the levels of individual isoforms were assessed on native PAGE. RESULTS: The LDH content was significantly higher in blood sera of sputum-positive (three positive) individuals (444+/-270 IU) as compared to sputum-negative samples (242+/-125 IU). Analysis on the association of different LDH isoforms (LDH1, 2, 3, 4 and 5) with sputum test revealed significantly higher frequency of LDH2 and LDH3 in sputum-positive samples as compared to sputum-negative samples. In sputum three positive cases, however, the frequency of LDH3 appeared much higher (in 60% cases) than LDH2 (in 33% cases), which was found to be almost same in sputum negative but higher in two positive samples (44.4%). CONCLUSIONS: The present data suggests a strong association of LDH3 with sputum three positive or severe cases of mycobacterial infection, indicating a likely possibility of using LDH3 as a supporting diagnostic marker in, at least, cases of chronic tuberculosis.     

皮肌炎患者血清LDH、CK及其同工酶分析

目的 探讨LDH、CK及其同工酶对皮肌炎的诊断及病程判断价值。方法 用日本HTTACHI747型全自动生化分析仪及美国REP全自动快速电泳系统检测57例皮肌炎患者血清LDH、CK同工酶。结果 57例皮肌炎患者82．21％LDH总酶升高，91．22％CK升高。在总酶升高的病例中，同工酶均有不同程度的特征性改变。结论 LDH、CK同工酶的检测对皮肌炎的诊断及其病程判断有重要价值。     

STUDIES ON THE IN VIVO AND IN VITRO MULTIPLICATION OF THE LDH VIRUS OF MICE

In vivo analysis of the virus titer in various loci, 24 hr after infection, showed that a titer similar to that in the blood plasma was found in the ascitic fluid of Erlich ascites cancer-bearing mice, and in lymph nodes, spleen, and thymus, i.e. loci which contain macrophages as a common cell type. However, only in the lymph nodes and in the ascitic fluid did the increase in virus titer precede or parallel the increase in the plasma. The LDH virus titer in the plasma of X-irradiated mice was similar to that of control mice, eliminating radiation-sensitive cells but not macrophages as target cells of the virus. Electron microscopic observation of infected lymph node cells revealed the presence of two types of particles: one consisting of small densely stained annuli, about 25 mµ in diameter and one of similar dense annuli with a halo extending the diameter to about 50 mµ. Such particles were repeatedly observed within single or double membraned vesicles. In vitro, the LDH virus multiplied only in cultures of mouse peritoneal macrophages, maintained in medium 199 with 10% FBS. The virus titer could be maintained for at least 33 days, during eleven serial passages, involving an overall dilution factor of 10¹¹. These results corroborate the findings of Evans and Salaman, who used peritoneal macrophages maintained in Eagle's medium and 5 to 10% lamb serum. However, in the serial passage experiments reported here, the virus titer could only be maintained following trypsinization of each successive inoculum. The role of macrophages as the target cell for LDH virus multiplication in vivo is discussed.     

益智灵对“血瘀”大鼠血液流变性的影响

益智灵主要由黄芪、丹参、菖蒲、灵芝等中草药组成。本文观察了它对“血瘀”大鼠血液流变性的影响。大鼠ｉｇ益智灵 ０．３２和 ０．６４ｇ／ｋｇ，连续 ７天。第 ７天用肾上腺素加冷刺激造成血瘀模型，再次给药２小时后测定血液粘度、红细胞压积和体外血栓 结果表明该药能非常显著地降低血瘀大鼠的全血粘度、血浆粘度、红细胞压积、血栓长度及血栓重量。     

补体C3对神经病理性疼痛模型脊髓星形胶质细胞的影响

目的:观察补体C3对神经病理性疼痛模型脊髓星形胶质细胞的影响。方法:81只补体C3基因敲除小鼠随机分三组(n=27):A组:假手术组;B组:慢性坐骨神经结扎模型(CCI)组;C组:CCI模型补体C3干预组。测定小鼠的热痛阈值和机械痛阈值,并取腰5、6脊髓节段测定GFAP mRNA和GFAP表达。结果 :术前三个组小鼠热和机械痛阈无明显差异,术后1天A组热和机械痛阈下降,其后恢复。B组和C组继续下降,C组下降幅度更大(P<0.05)。术后第1天,B组和C组胶质细胞激活轻微,术后第3、7天B组和C组脊髓组织GFAP mRNA和GFAP表达量逐渐增加,且C组其表达量明显高于B组。结论 :补体C3的存在与神经病理性模型小鼠星形胶质细胞激活显著相关,并由此影响到慢性疼痛状态的出现和维持。     

降纤酶对大鼠血液流变性影响的研究

目的 旨在观察降纤酶对大鼠急性高粘血症血液流变性的影响。方法 将ＳＤ大鼠４０只分为５组,即正常对照组、高粘血症模型组、降纤酶大剂量组（０．８ｕ／ｋｇ）、降纤酶小剂量组（０．４ｕ／ｋｇ）、丹参阳性对照组（０．７２ｇ／ｋｇ）,用皮下注射肾上腺素加冷水浴刺激做高血症模型。结果 降纤酶能非常显著地降低全血低粘度（ηｂＬ）、高切粘度（ηｂＨ）、血浆纤维蛋白原浓度（ＰＦＣ）、血浆粘度（ηｐ）全血低切还原粘度（ηｒＬ）,并能显著降低红细胞压积（Ｈｃｔ）、全血高切还原粘度（ηｒＨ）、红细胞聚集指数（ＥＡＩ）,但对红细胞刚性指数（ＥＲＩ）无影响。而丹参仅能轻度降低ηｂＬ、ηｐ、ＨＣＴ及ＰＦＣ。结论 提示降纤酶能改善高粘血症血液流变学特性,可用来防治冠心病伴高纤维蛋白原血症。     

EFFECTS OF FIRES ON VEGETATION IN THE NORTHERN TERRITORY

Annual burning is practised over nearly the whole of that part of the Northern Territory which has an annual rainfall of more than thirty-five inches. The remainder of the Territory is burnt at greater intervals.

Before the arrival of aboriginals fires would have been lit by natural agencies, especially lightning. The aboriginals used fires extensively for hunting and access clearing. Europeans have adopted a burning regime similar to that established by the aboriginals, for fire has been found to be a cheap and valuable tool in the management of much of the Territory's range lands.

The vegetation of the Territory in the area of annual or frequent burning appears well suited to regular burning, and is probably dependent upon fire for its maintenance. The vegetation in arid and semi-arid areas is more fire-sensitive. The interval between fires in these areas may be considerable because unusually heavy rainfalls are required to produce enough grass to carry a fire. Fires rarely occur in the small areas of monsoon forest.